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The map label for this gene is dapF

Identifier: 77461720

GI number: 77461720

Start: 6165723

End: 6166553

Strand: Direct

Name: dapF

Synonym: Pfl01_5499

Alternate gene names: 77461720

Gene position: 6165723-6166553 (Clockwise)

Preceding gene: 77461719

Following gene: 77461721

Centisome position: 95.76

GC content: 63.66

Gene sequence:

>831_bases
ATGCTGCTGCGTTTTACCAAGATGCATGGCCTGGGCAACGACTTCATGGTTCTCGACCTGGTCAGTCAGCACGCGCATAT
TCAGCCCAAGCACGCGAAAATGTGGGGCGACCGGCACACCGGCATCGGTTTCGACCAGTTGCTGATCGTCGAGGCGCCAA
GCAATCCGGACGTGGATTTCCGTTACCGGATCTTCAACTCCGACGGCTCCGAAGTGGAGCAGTGCGGCAACGGTGCACGC
TGCTTCGCCCGCTTCGTGCTTGATAAACGCCTGACCGCCAAACGGCAGATCCGCGTCGAGACCAAGGGCGGCATCATTGA
GCTGGACGTGCGTAACGACGGCCAGATCGGCGTGAACATGGGCGCCCCGCGCCTGGTGCCGGCGGACATCCCGTTCCAGG
CGCCGGAGCAGGCCCTGAGTTATCAGGTCGATGTCGACGGTACGCCGGTCGATCTGGCGGCGGTGTCCATGGGCAACCCC
CATGCGGTGCTGCGGGTCAGCGATATCAACACTGCACCGGTGCATGAACTGGGGCCGAAAATCGAACACCATCCGCGCTT
CCCGGCGCGGGTCAACGTCGGTTTTCTCCAGGTCATCGACCGCCATCGCGCGCAACTGCGCGTGTGGGAACGCGGCGCCG
GGGAAACCCAGGCCTGCGGCACCGGTGCCTGCGCTGCTGCTGTAGCGGCGATCAGTCAGGGGTGGATGGATTCGCCACTG
TTGATCGACCTGCCGGGCGGGCGTTTGTCCATAGAGTGGGCAGGCCCCGGCCAACCGGTGTTGATGACCGGCCCGGCAGT
GCGCGTATACGAAGGACAAGTGCGTCTTTGA

Upstream 100 bases:

>100_bases
GCCGAAGTGCTGGTGGATGGCGATCAGGCCTTTGAAGTGCGTCGCCGCGAAACCGTGGCCGAGCTGTTTGCCGGCGAAAG
CCTGCTGCCGGAGTGACCCC

Downstream 100 bases:

>100_bases
GTGAGCAGAAGCCATGACCGATAAGCCTCAGGTACCCGCCCGACAGTCCGGTGAATCAGCGTCCGAGAGCCTGGAGGCGG
CAGCAGTTGCCGCGTACCTG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MLLRFTKMHGLGNDFMVLDLVSQHAHIQPKHAKMWGDRHTGIGFDQLLIVEAPSNPDVDFRYRIFNSDGSEVEQCGNGAR
CFARFVLDKRLTAKRQIRVETKGGIIELDVRNDGQIGVNMGAPRLVPADIPFQAPEQALSYQVDVDGTPVDLAAVSMGNP
HAVLRVSDINTAPVHELGPKIEHHPRFPARVNVGFLQVIDRHRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
LIDLPGGRLSIEWAGPGQPVLMTGPAVRVYEGQVRL

Sequences:

>Translated_276_residues
MLLRFTKMHGLGNDFMVLDLVSQHAHIQPKHAKMWGDRHTGIGFDQLLIVEAPSNPDVDFRYRIFNSDGSEVEQCGNGAR
CFARFVLDKRLTAKRQIRVETKGGIIELDVRNDGQIGVNMGAPRLVPADIPFQAPEQALSYQVDVDGTPVDLAAVSMGNP
HAVLRVSDINTAPVHELGPKIEHHPRFPARVNVGFLQVIDRHRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
LIDLPGGRLSIEWAGPGQPVLMTGPAVRVYEGQVRL
>Mature_276_residues
MLLRFTKMHGLGNDFMVLDLVSQHAHIQPKHAKMWGDRHTGIGFDQLLIVEAPSNPDVDFRYRIFNSDGSEVEQCGNGAR
CFARFVLDKRLTAKRQIRVETKGGIIELDVRNDGQIGVNMGAPRLVPADIPFQAPEQALSYQVDVDGTPVDLAAVSMGNP
HAVLRVSDINTAPVHELGPKIEHHPRFPARVNVGFLQVIDRHRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
LIDLPGGRLSIEWAGPGQPVLMTGPAVRVYEGQVRL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=54.7445255474453, Blast_Score=319, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_PSEPF (Q3K4R8)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_351227.1
- HSSP:   P44859
- ProteinModelPortal:   Q3K4R8
- SMR:   Q3K4R8
- STRING:   Q3K4R8
- GeneID:   3713823
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5499
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   PFLU205922:PFL_5499-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30157; Mature: 30157

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRFTKMHGLGNDFMVLDLVSQHAHIQPKHAKMWGDRHTGIGFDQLLIVEAPSNPDVDF
CEEEEEECCCCCCCEEEEEEHHHCCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCEE
RYRIFNSDGSEVEQCGNGARCFARFVLDKRLTAKRQIRVETKGGIIELDVRNDGQIGVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCEEEEEC
GAPRLVPADIPFQAPEQALSYQVDVDGTPVDLAAVSMGNPHAVLRVSDINTAPVHELGPK
CCCEEEECCCCCCCCHHHHEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHCCC
IEHHPRFPARVNVGFLQVIDRHRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
CCCCCCCCCEECHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
LIDLPGGRLSIEWAGPGQPVLMTGPAVRVYEGQVRL
EEECCCCEEEEEECCCCCCEEEECCEEEEECCEECC
>Mature Secondary Structure
MLLRFTKMHGLGNDFMVLDLVSQHAHIQPKHAKMWGDRHTGIGFDQLLIVEAPSNPDVDF
CEEEEEECCCCCCCEEEEEEHHHCCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCEE
RYRIFNSDGSEVEQCGNGARCFARFVLDKRLTAKRQIRVETKGGIIELDVRNDGQIGVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCEEEEEC
GAPRLVPADIPFQAPEQALSYQVDVDGTPVDLAAVSMGNPHAVLRVSDINTAPVHELGPK
CCCEEEECCCCCCCCHHHHEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHCCC
IEHHPRFPARVNVGFLQVIDRHRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
CCCCCCCCCEECHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
LIDLPGGRLSIEWAGPGQPVLMTGPAVRVYEGQVRL
EEECCCCEEEEEECCCCCCEEEECCEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA