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The map label for this gene is yeiE [H]

Identifier: 77461591

GI number: 77461591

Start: 6031902

End: 6032765

Strand: Reverse

Name: yeiE [H]

Synonym: Pfl01_5370

Alternate gene names: 77461591

Gene position: 6032765-6031902 (Counterclockwise)

Preceding gene: 77461592

Following gene: 77461590

Centisome position: 93.7

GC content: 64.24

Gene sequence:

>864_bases
GTGCTGAGTGCCGAGCTGAAAGCGTTTTACATGGTGGCCCGCTTGGGCAGCATCACGCTGGCGGCGAAAAAGCTCGGCCT
CAGCCAACCGACCGTGACCACCCAGATCCGCAATCTGGAAAGCCAGTATTCGGTAGAGCTGTTCTACCGTGGCGGCCGGC
GCCTGAGTGTCAGCGACGAAGGTGCGCGGCTGTTGCCGATGGTCAAGGCGTTGTTGCAGCAGGAGGCCGACATCGAATTC
TTCCTGCGCAACAGTGGCCAGGTGCAGGGTGCGCTGCGCATCGCCGCCACCGCGCCGTATTACATCCTCGATCTGGTGAA
GACCTTCCGCGAGCGCCTGCCGCAGGTGGAGGTGTCGGTGGAAATCGGCAACTCGCAGCAGGTGCTCGAAGCGCTGGAGG
ATTATCGGGTGGATGTCGCGGCGTCGTCGCAATTGCTCGACGATGCCCGGCTGATCCGCCGGGTGCTCGGCAGCGACCCG
CTGGTGCTGGCGGTGCATCGCAATCATCCGCTGGCGGCGCACGACCATGTGACGCTGAGCGCGCTGGCCGGACATACCTT
GCTGATGCGCGAACCGGGTTCGACCACTCGACGGCTCACCGAAGAGTTGCTGGCCAGCGCCGGCGTCAGTTTTGGCCCGT
TGCTGGAGATAGGCAGCCGCGAATCGATCCGCGAGGCGGTGCTGCGCAACATCGGCATCAGCATCATCGCCCGCCAGGAA
GTGCCGCACGATCCGCAGTTGCGGGTGCTGACCCTGGAGAATGCGCCGCAGATTCCCGAATACCTCTACTGCCTCAAGGA
ACGCAAGAACGCCCGGTTGCCGGCGGCGTTTCTCGGACTGGCGCAGGAAATGTCCCCGGCCTGA

Upstream 100 bases:

>100_bases
GCCACCATGTGGATGGCGGTGTTTGCCGACATGGGCGTGAGCCTGCTGGTGGTGTTCAACGGTTTGCGCCTGCTGCGCAA
ATAGAGGAAGAGGGGCGGTT

Downstream 100 bases:

>100_bases
TCAACCAAAATCCCTGAATACCACTATCGGTCGTTTTTGCCTCACTGCCATATGACCGACGCATTACAACTCTAGGATTC
GTCTCATCTGCTTGATGAGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLSAELKAFYMVARLGSITLAAKKLGLSQPTVTTQIRNLESQYSVELFYRGGRRLSVSDEGARLLPMVKALLQQEADIEF
FLRNSGQVQGALRIAATAPYYILDLVKTFRERLPQVEVSVEIGNSQQVLEALEDYRVDVAASSQLLDDARLIRRVLGSDP
LVLAVHRNHPLAAHDHVTLSALAGHTLLMREPGSTTRRLTEELLASAGVSFGPLLEIGSRESIREAVLRNIGISIIARQE
VPHDPQLRVLTLENAPQIPEYLYCLKERKNARLPAAFLGLAQEMSPA

Sequences:

>Translated_287_residues
MLSAELKAFYMVARLGSITLAAKKLGLSQPTVTTQIRNLESQYSVELFYRGGRRLSVSDEGARLLPMVKALLQQEADIEF
FLRNSGQVQGALRIAATAPYYILDLVKTFRERLPQVEVSVEIGNSQQVLEALEDYRVDVAASSQLLDDARLIRRVLGSDP
LVLAVHRNHPLAAHDHVTLSALAGHTLLMREPGSTTRRLTEELLASAGVSFGPLLEIGSRESIREAVLRNIGISIIARQE
VPHDPQLRVLTLENAPQIPEYLYCLKERKNARLPAAFLGLAQEMSPA
>Mature_287_residues
MLSAELKAFYMVARLGSITLAAKKLGLSQPTVTTQIRNLESQYSVELFYRGGRRLSVSDEGARLLPMVKALLQQEADIEF
FLRNSGQVQGALRIAATAPYYILDLVKTFRERLPQVEVSVEIGNSQQVLEALEDYRVDVAASSQLLDDARLIRRVLGSDP
LVLAVHRNHPLAAHDHVTLSALAGHTLLMREPGSTTRRLTEELLASAGVSFGPLLEIGSRESIREAVLRNIGISIIARQE
VPHDPQLRVLTLENAPQIPEYLYCLKERKNARLPAAFLGLAQEMSPA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=273, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI145693105, Length=246, Percent_Identity=27.2357723577236, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082132, Length=226, Percent_Identity=29.646017699115, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=30.4794520547945, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788748, Length=279, Percent_Identity=27.5985663082437, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790399, Length=226, Percent_Identity=25.6637168141593, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786401, Length=251, Percent_Identity=29.8804780876494, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788296, Length=230, Percent_Identity=27.3913043478261, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787530, Length=237, Percent_Identity=25.3164556962025, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31726; Mature: 31726

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAELKAFYMVARLGSITLAAKKLGLSQPTVTTQIRNLESQYSVELFYRGGRRLSVSDE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCC
GARLLPMVKALLQQEADIEFFLRNSGQVQGALRIAATAPYYILDLVKTFRERLPQVEVSV
CCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEE
EIGNSQQVLEALEDYRVDVAASSQLLDDARLIRRVLGSDPLVLAVHRNHPLAAHDHVTLS
EECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEHH
ALAGHTLLMREPGSTTRRLTEELLASAGVSFGPLLEIGSRESIREAVLRNIGISIIARQE
HHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHCCEEEEEECC
VPHDPQLRVLTLENAPQIPEYLYCLKERKNARLPAAFLGLAQEMSPA
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLSAELKAFYMVARLGSITLAAKKLGLSQPTVTTQIRNLESQYSVELFYRGGRRLSVSDE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEEEECCCEEEECCC
GARLLPMVKALLQQEADIEFFLRNSGQVQGALRIAATAPYYILDLVKTFRERLPQVEVSV
CCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEE
EIGNSQQVLEALEDYRVDVAASSQLLDDARLIRRVLGSDPLVLAVHRNHPLAAHDHVTLS
EECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEHH
ALAGHTLLMREPGSTTRRLTEELLASAGVSFGPLLEIGSRESIREAVLRNIGISIIARQE
HHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHCCEEEEEECC
VPHDPQLRVLTLENAPQIPEYLYCLKERKNARLPAAFLGLAQEMSPA
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]