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The map label for this gene is potD [C]

Identifier: 77461588

GI number: 77461588

Start: 6027921

End: 6028946

Strand: Reverse

Name: potD [C]

Synonym: Pfl01_5367

Alternate gene names: 77461588

Gene position: 6028946-6027921 (Counterclockwise)

Preceding gene: 77461589

Following gene: 77461587

Centisome position: 93.64

GC content: 62.48

Gene sequence:

>1026_bases
ATGTTCAAGCCTATGGCCCTGGCCGCTGCTGTGCTCGCTGCTTTCAGCCTGAATGCCTTCGCGGCAAAAACCGAGTTGAC
GGTGTACACCGCCCTCGAAGCCGAGCAGTTGAAGTCCTACAAAGAGGCCTTCGAAAAGGCCAACCCGGACGTCGAGATCA
AGTGGGTGCGCGACTCCACCGGGATCATCACCGCCAAACTGCTGGCCGAAAAGGATCGCCCGCAGGCTGACGCGGTGTGG
GGCCTGGCAGCATCGAGCCTGGCGATCCTCGATCAACAAGGCATGCTGCAAAGCTATGCGCCGAAGGATCTGGGCAAGAT
CGGCGCGAACTACCGCGACGCCGCCAACCCGCCAGCCTGGGTCGGCATGGACGTCTGGGCCGCGACGATTTGCTTCAACA
CCGTCGAGGCCGAGAAACAGGGCCTGAGCAAACCGGTGAGCTGGCAGGACCTGACCAAGCCTGAGTACAAGGGCAAGATC
GTGATGCCGAACCCGGCTTCGTCCGGCACCGGTTTCCTCGACGTCAGCGCCTGGCTGCAAACCTTCGGCGAGAAACAGGG
CTGGGCCTACATGGACGGCCTGCACCAGAACATCGGCCAGTACGTTCACTCCGGTTCCAAGCCTTGCAAACTGGCGGCGG
CGGGCGAATTCCCGATCGGCATTTCCTTCGAATACCCGGCCGTTCAGCTCAAGCGTCAGGGCGCGCCGCTGGACATCATC
CTGCCGAAGGAAGGCCTGGGCTGGGAGATCGAAGCGACCGCCGTGATCAAAGGCACGCCGCATGAAGAGGCCGCGAAGAA
ACTGGCCGACTTCTCCGCCAGCGCCCCGGCGATGGACCTGTACAAGGAAAACTTCGCTGTCCTCGCCCAGCCGGGCATCG
CCAAGCCGCAGACCGAACTGCCGGCCGACTACGAGCAGCGCCTGATCAAGAACGACTTTGCCTGGGCCTCGAAGAACCGC
GACGAGATCCTGAGCGAATGGCGCAAGCGCTATGACGGCAAGTCCGAGAAAGTCGCGGCCAAGTAA

Upstream 100 bases:

>100_bases
GCGGCTTGCTGCGCCGTTCCCAGGCCTGGCGGCAAACGGCGCCCGGCCACTGATCCACGACTTATCCCTTCAGCCTCAAC
TCACCGACAGGAAAACGATC

Downstream 100 bases:

>100_bases
GTTCTTCATCGACTGACAGGGCCCCATCGCTGGCAAGCCAGCTCCCACAGGTTTGGCGGCGTTCGCACAATGTGTATTCC
GCACCGGGCAATGTGGGAGC

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MFKPMALAAAVLAAFSLNAFAAKTELTVYTALEAEQLKSYKEAFEKANPDVEIKWVRDSTGIITAKLLAEKDRPQADAVW
GLAASSLAILDQQGMLQSYAPKDLGKIGANYRDAANPPAWVGMDVWAATICFNTVEAEKQGLSKPVSWQDLTKPEYKGKI
VMPNPASSGTGFLDVSAWLQTFGEKQGWAYMDGLHQNIGQYVHSGSKPCKLAAAGEFPIGISFEYPAVQLKRQGAPLDII
LPKEGLGWEIEATAVIKGTPHEEAAKKLADFSASAPAMDLYKENFAVLAQPGIAKPQTELPADYEQRLIKNDFAWASKNR
DEILSEWRKRYDGKSEKVAAK

Sequences:

>Translated_341_residues
MFKPMALAAAVLAAFSLNAFAAKTELTVYTALEAEQLKSYKEAFEKANPDVEIKWVRDSTGIITAKLLAEKDRPQADAVW
GLAASSLAILDQQGMLQSYAPKDLGKIGANYRDAANPPAWVGMDVWAATICFNTVEAEKQGLSKPVSWQDLTKPEYKGKI
VMPNPASSGTGFLDVSAWLQTFGEKQGWAYMDGLHQNIGQYVHSGSKPCKLAAAGEFPIGISFEYPAVQLKRQGAPLDII
LPKEGLGWEIEATAVIKGTPHEEAAKKLADFSASAPAMDLYKENFAVLAQPGIAKPQTELPADYEQRLIKNDFAWASKNR
DEILSEWRKRYDGKSEKVAAK
>Mature_341_residues
MFKPMALAAAVLAAFSLNAFAAKTELTVYTALEAEQLKSYKEAFEKANPDVEIKWVRDSTGIITAKLLAEKDRPQADAVW
GLAASSLAILDQQGMLQSYAPKDLGKIGANYRDAANPPAWVGMDVWAATICFNTVEAEKQGLSKPVSWQDLTKPEYKGKI
VMPNPASSGTGFLDVSAWLQTFGEKQGWAYMDGLHQNIGQYVHSGSKPCKLAAAGEFPIGISFEYPAVQLKRQGAPLDII
LPKEGLGWEIEATAVIKGTPHEEAAKKLADFSASAPAMDLYKENFAVLAQPGIAKPQTELPADYEQRLIKNDFAWASKNR
DEILSEWRKRYDGKSEKVAAK

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: COG1840

COG function: function code P; ABC-type Fe3+ transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37273; Mature: 37273

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKPMALAAAVLAAFSLNAFAAKTELTVYTALEAEQLKSYKEAFEKANPDVEIKWVRDST
CCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCC
GIITAKLLAEKDRPQADAVWGLAASSLAILDQQGMLQSYAPKDLGKIGANYRDAANPPAW
CEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHCCCCCCCCCCCCE
VGMDVWAATICFNTVEAEKQGLSKPVSWQDLTKPEYKGKIVMPNPASSGTGFLDVSAWLQ
ECHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEECCCCCCCCCEEEHHHHHH
TFGEKQGWAYMDGLHQNIGQYVHSGSKPCKLAAAGEFPIGISFEYPAVQLKRQGAPLDII
HHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCHHHHCCCCCCEEEE
LPKEGLGWEIEATAVIKGTPHEEAAKKLADFSASAPAMDLYKENFAVLAQPGIAKPQTEL
ECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCCCCCC
PADYEQRLIKNDFAWASKNRDEILSEWRKRYDGKSEKVAAK
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFKPMALAAAVLAAFSLNAFAAKTELTVYTALEAEQLKSYKEAFEKANPDVEIKWVRDST
CCCHHHHHHHHHHHHHHHHHHHHCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCC
GIITAKLLAEKDRPQADAVWGLAASSLAILDQQGMLQSYAPKDLGKIGANYRDAANPPAW
CEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHCCCCCCCCCCCCE
VGMDVWAATICFNTVEAEKQGLSKPVSWQDLTKPEYKGKIVMPNPASSGTGFLDVSAWLQ
ECHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEECCCCCCCCCEEEHHHHHH
TFGEKQGWAYMDGLHQNIGQYVHSGSKPCKLAAAGEFPIGISFEYPAVQLKRQGAPLDII
HHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCHHHHCCCCCCEEEE
LPKEGLGWEIEATAVIKGTPHEEAAKKLADFSASAPAMDLYKENFAVLAQPGIAKPQTEL
ECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECCCCCCCCCCC
PADYEQRLIKNDFAWASKNRDEILSEWRKRYDGKSEKVAAK
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]