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The map label for this gene is rpoH [H]

Identifier: 77461555

GI number: 77461555

Start: 5993288

End: 5994142

Strand: Reverse

Name: rpoH [H]

Synonym: Pfl01_5334

Alternate gene names: 77461555

Gene position: 5994142-5993288 (Counterclockwise)

Preceding gene: 77461556

Following gene: 77461553

Centisome position: 93.1

GC content: 60.0

Gene sequence:

>855_bases
ATGTCCAATTCTTTGCAACCTGCGTATGCATTGGTTCCAGGCGCAAACCTGGAAGCCTACGTGCACACCGTCAACAGCAT
TCCATTGCTGACGCCGGAGCAGGAGCGTGAACTGGCCGAGAGTCTCTACTATGAGCAGGATCTGGGGGCGGCTCGGCAGA
TGGTGCTCGCCCACCTGCGGTTTGTCGTACACATCGCCCGTAGCTATTCCGGCTACGGTCTGGCTCAGGCTGACCTGATC
CAGGAAGGTAACGTCGGCCTGATGAAGGCCGTGAAGCGTTTCAACCCGGAAATGGGCGTGCGTCTGGTGTCCTTCGCGGT
GCACTGGATCAAGGCCGAGATTCACGAGTTCATCCTGCGCAACTGGCGGATCGTGAAAGTCGCGACCACCAAGGCCCAGC
GCAAGCTGTTCTTCAACCTGCGCAGCCAGAAGAAACGTCTGGCCTGGCTGAACAACGAGGAAGTCCACCGTGTGGCGGAA
AGCCTCGGCGTCGAGCCGCGTGAAGTGCGTGAGATGGAAAGTCGCCTGACCGGCCATGACATGGCCTTCGACCCGGCTGC
GGAAGCGGACGACGACAGTGCTTTCCAGTCGCCGGCCAACTACCTGGAAGACCACCGGTACGACCCGGCGCGTCAACTGG
AAGACGCCGACTGGAGCGACAACTCCAATCACAACCTGCACGAAGCGCTGGAAGTGCTGGACGAACGCAGCCGCGACATC
CTCTACCAGCGCTGGCTGGCAGAAGAGAAAGCCACGCTGCACGACCTGGCGCAGAAGTACAACGTCTCGGCCGAGCGGAT
CCGTCAGCTCGAGAAGAGTGCGATGAACAAGCTCAAGTTGTCGATTGCCGCATAA

Upstream 100 bases:

>100_bases
TGATTGACCTTTCAGTGGTTAAGGAAACTTTCATTTGAGTTTCCGGTCAATTTTTCGCAATGCTGAAAAGCATGAGTATG
TAAGTCGGAGGTTTTTCCGT

Downstream 100 bases:

>100_bases
CCGGTAGTCCGGCAATAAAAAACGCCCCGATCAGTGATGATCGGGGCGTTTTTGTTTGCCTGTCAGAACGTCATTGGGCC
CAGGGCGCCCGACGTGAATC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSNSLQPAYALVPGANLEAYVHTVNSIPLLTPEQERELAESLYYEQDLGAARQMVLAHLRFVVHIARSYSGYGLAQADLI
QEGNVGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEFILRNWRIVKVATTKAQRKLFFNLRSQKKRLAWLNNEEVHRVAE
SLGVEPREVREMESRLTGHDMAFDPAAEADDDSAFQSPANYLEDHRYDPARQLEDADWSDNSNHNLHEALEVLDERSRDI
LYQRWLAEEKATLHDLAQKYNVSAERIRQLEKSAMNKLKLSIAA

Sequences:

>Translated_284_residues
MSNSLQPAYALVPGANLEAYVHTVNSIPLLTPEQERELAESLYYEQDLGAARQMVLAHLRFVVHIARSYSGYGLAQADLI
QEGNVGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEFILRNWRIVKVATTKAQRKLFFNLRSQKKRLAWLNNEEVHRVAE
SLGVEPREVREMESRLTGHDMAFDPAAEADDDSAFQSPANYLEDHRYDPARQLEDADWSDNSNHNLHEALEVLDERSRDI
LYQRWLAEEKATLHDLAQKYNVSAERIRQLEKSAMNKLKLSIAA
>Mature_283_residues
SNSLQPAYALVPGANLEAYVHTVNSIPLLTPEQERELAESLYYEQDLGAARQMVLAHLRFVVHIARSYSGYGLAQADLIQ
EGNVGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEFILRNWRIVKVATTKAQRKLFFNLRSQKKRLAWLNNEEVHRVAES
LGVEPREVREMESRLTGHDMAFDPAAEADDDSAFQSPANYLEDHRYDPARQLEDADWSDNSNHNLHEALEVLDERSRDIL
YQRWLAEEKATLHDLAQKYNVSAERIRQLEKSAMNKLKLSIAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=285, Percent_Identity=58.9473684210526, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI1789098, Length=263, Percent_Identity=30.0380228136882, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32605; Mature: 32474

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSLQPAYALVPGANLEAYVHTVNSIPLLTPEQERELAESLYYEQDLGAARQMVLAHLR
CCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVVHIARSYSGYGLAQADLIQEGNVGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEFILR
HHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NWRIVKVATTKAQRKLFFNLRSQKKRLAWLNNEEVHRVAESLGVEPREVREMESRLTGHD
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCC
MAFDPAAEADDDSAFQSPANYLEDHRYDPARQLEDADWSDNSNHNLHEALEVLDERSRDI
CCCCCCCCCCCCHHHHCHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LYQRWLAEEKATLHDLAQKYNVSAERIRQLEKSAMNKLKLSIAA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNSLQPAYALVPGANLEAYVHTVNSIPLLTPEQERELAESLYYEQDLGAARQMVLAHLR
CCCCCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVVHIARSYSGYGLAQADLIQEGNVGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEFILR
HHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
NWRIVKVATTKAQRKLFFNLRSQKKRLAWLNNEEVHRVAESLGVEPREVREMESRLTGHD
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCC
MAFDPAAEADDDSAFQSPANYLEDHRYDPARQLEDADWSDNSNHNLHEALEVLDERSRDI
CCCCCCCCCCCCHHHHCHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LYQRWLAEEKATLHDLAQKYNVSAERIRQLEKSAMNKLKLSIAA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7728657; 7698670; 10984043 [H]