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The map label for this gene is opuAB [H]

Identifier: 77461460

GI number: 77461460

Start: 5895132

End: 5895995

Strand: Direct

Name: opuAB [H]

Synonym: Pfl01_5239

Alternate gene names: 77461460

Gene position: 5895132-5895995 (Clockwise)

Preceding gene: 77461459

Following gene: 77461461

Centisome position: 91.56

GC content: 61.11

Gene sequence:

>864_bases
ATGCGGACGTTCACTACCATGCTGATTGATCAGAAAATCCCTTTAGGCCAGTACATCGCGGGCTTCGTTGAATGGTTGAC
GCAACACGGCGCCAACACCTTCGACGCAATCGCCGTGACACTGGAAACGATGATCCACGGCGTGACGTTTGCGCTGACCT
GGTTCAACCCGCTGGTGCTGATCGGCCTCATCGCCCTGCTGGCCCACTTCATTCAACGCAAATGGGGCCTGACCGCGTTC
GTGGTCGCTTCCTTCCTGCTGATCCTCAACCTGGGGTACTGGCAGGAAACCATGGAAACCCTCGCCCAGGTCATGTTCGC
GACCCTGGTCTGCGTGGTCATCGGCGTGCCGCTGGGCATCGTCGCCGCGCACAAACCGATGTTCTACACTTTGATGAGGC
CGGTACTCGATCTGATGCAGACCGTACCGACCTTCGTTTACCTCATTCCTACCCTGACCCTCTTCGGGCTGGGCGTGGTG
CCGGGCCTGATCTCGACGGTGGTGTTCGCCATCGCCGCGCCGATCCGCCTGACCTACCTGGGCATCCGCGATGTCCCGCA
AGAACTGATGGACGCCGGCAAGGCCTTCGGCTGCTCGCGTCGCCAACTGCTTTCACGGATCGAACTGCCTCACGCCATGC
CGAGCATCGCGGCCGGCATCACCCAGTGCATCATGCTGTCGCTGTCGATGGTGGTGATCGCGGCACTGGTGGGCGCCGAC
GGACTCGGCAAACCGGTGGTCAACGCACTGAACACTGCTGATATCGCCCTGGGCTTCGAAGCGGGCCTGGCGATCGTACT
GCTGGCGATCATGCTCGACCGTATCTGCAAACAACCCGACGCCAAAGCAGGGGGTGACGCATGA

Upstream 100 bases:

>100_bases
ATTGACGGTAAACCTGGCCTGGAGGCCGTGAAAGCCAAGATTGCCCAGTAATCGCTTATGCCGGGCGGGCTAGTCCCGTC
CGGGCTGTTTATTTCCTTGC

Downstream 100 bases:

>100_bases
GCATAATTCGCTTCGATAACGTTGACGTGATCTTCACCAAGGATCCGCGCGAAGCACTGAAACTTCTCGATCAAGGCCTG
ACCCGCAGTGAAATCCTGAA

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRTFTTMLIDQKIPLGQYIAGFVEWLTQHGANTFDAIAVTLETMIHGVTFALTWFNPLVLIGLIALLAHFIQRKWGLTAF
VVASFLLILNLGYWQETMETLAQVMFATLVCVVIGVPLGIVAAHKPMFYTLMRPVLDLMQTVPTFVYLIPTLTLFGLGVV
PGLISTVVFAIAAPIRLTYLGIRDVPQELMDAGKAFGCSRRQLLSRIELPHAMPSIAAGITQCIMLSLSMVVIAALVGAD
GLGKPVVNALNTADIALGFEAGLAIVLLAIMLDRICKQPDAKAGGDA

Sequences:

>Translated_287_residues
MRTFTTMLIDQKIPLGQYIAGFVEWLTQHGANTFDAIAVTLETMIHGVTFALTWFNPLVLIGLIALLAHFIQRKWGLTAF
VVASFLLILNLGYWQETMETLAQVMFATLVCVVIGVPLGIVAAHKPMFYTLMRPVLDLMQTVPTFVYLIPTLTLFGLGVV
PGLISTVVFAIAAPIRLTYLGIRDVPQELMDAGKAFGCSRRQLLSRIELPHAMPSIAAGITQCIMLSLSMVVIAALVGAD
GLGKPVVNALNTADIALGFEAGLAIVLLAIMLDRICKQPDAKAGGDA
>Mature_287_residues
MRTFTTMLIDQKIPLGQYIAGFVEWLTQHGANTFDAIAVTLETMIHGVTFALTWFNPLVLIGLIALLAHFIQRKWGLTAF
VVASFLLILNLGYWQETMETLAQVMFATLVCVVIGVPLGIVAAHKPMFYTLMRPVLDLMQTVPTFVYLIPTLTLFGLGVV
PGLISTVVFAIAAPIRLTYLGIRDVPQELMDAGKAFGCSRRQLLSRIELPHAMPSIAAGITQCIMLSLSMVVIAALVGAD
GLGKPVVNALNTADIALGFEAGLAIVLLAIMLDRICKQPDAKAGGDA

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=276, Percent_Identity=40.5797101449275, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788449, Length=194, Percent_Identity=28.3505154639175, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788451, Length=183, Percent_Identity=30.0546448087432, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786564, Length=158, Percent_Identity=31.6455696202532, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30939; Mature: 30939

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]