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The map label for this gene is stcD [H]

Identifier: 77461444

GI number: 77461444

Start: 5878235

End: 5880295

Strand: Reverse

Name: stcD [H]

Synonym: Pfl01_5223

Alternate gene names: 77461444

Gene position: 5880295-5878235 (Counterclockwise)

Preceding gene: 77461445

Following gene: 77461443

Centisome position: 91.33

GC content: 62.01

Gene sequence:

>2061_bases
ATGGCTTTCGAAGCAATGTTTCAGCCAATTCAAATTGGCAAACTGACCATCCGCAACCGCGTGCTCAGCACCGCGCACGC
CGAGGTCTACGCGACCGACGGCGGCATGACCACCGAGCGGTACGTCAAGTATTACGAAGAGAAAGCCAAGGGCGGCATCG
GCCTGGCGATCTGTGGCGGTTCGTCCGTGGTGGCGATCGACAGCCCGCAGCAATGGTGGAGTTCGGTGAACCTGTCCACC
GACCGGATCATCCCGCACTTCCAGAATCTCGCCGACGCCATGCACAAGCACGGCGCCAAGATCATGATCCAGATTACCCA
CATGGGCCGTCGCTCCCGTTGGGACGGTTTCCACTGGCCGACCCTGATGTCGCCGTCGGGTATCCGCGAACCGGTGCACC
GCGCCACCTGCAAGACCATCGAGCCGGAAGAAATCTGGCGGGTGATCGGTAACTACGCGCAAGCCGCGCGCCGCGCCAAG
GCCGGTGGCCTGGACGGCGTCGAACTGTCGGCCGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCGCGCGTCAACAA
GCGGACCGACGAATGGGGCGGCACTTTCGAGGGCCGCATGAAGTTCGGTCTGGAAGTCCTGAAAGCCGTACGCGCCGAGG
TAGGTGACGACTTCTGCGTCGGCATGCGCATCTGCGGTGACGAGTTCCACCCGGACGGTCTGTCCCACGAAGACATGAAG
CAGATCGCCAAATATTACGACGACACCGGCATGCTCGATTTCATCGGCGTCGTCGGCTCGGGTTGCGACACCCACAACAC
CCTGGCCAACGTGATTCCGAACATGAGTTATCCGCCGGAGCCGTTCCTGCACCTGGCGGCCGGAATCAAGGAAGTGGTCA
AGGTTCCGGTGCTGCACGCGCAGAACATCAAGGACCCGAACCAGGCCACGCGGATTCTCGAGGGCGGTTACGTCGACATG
GTCGGCATGACCCGCGCCCACATCGCCGACCCGCACCTGATCGCCAAGATCAAGATGGGCCAGATCGACCAGATCAAACA
GTGCGTCGGCGCCAACTACTGCATCGACCGTCAGTATCAAGGTCTGGACGTGCTGTGCATCCAGAACGCCGCGACCTCCC
GTGAATACATGGGCGTGCCGCACATCATCGAGAAATCCACCGGGCCGAAACGCAAGGTGGTGATCGTCGGTGCCGGCCCG
GCGGGGATGGAAGCTGCCCGTGTGGCGGCCGAACGTGGCCACGACGTGACCCTGTTCGAGAAGAAGGAATTCATCGGCGG
CCAGATCACTACGGCCTCGAAAGCGCCGCAGCGTGACCAGATCGCCGGCATCACCCGCTGGTTCCAGCTGGAGCTGGCAC
GGCTGAAAGTCGACCTGCGCCTGGGCACTGCCGCAGACGCCGACACCATCATGGACCTGCGCCCGGACATCGTGGTGCTG
GCGGTTGGCGGGCATCCATACCTTGAACAGAACGAACACTGGGGCGCCGCCGAAGGGCTGGTGGTCAGCAGCTGGGACGT
GCTCGACGGCAAGGTCGCGCCGGGCAAGAACGTGCTGGTCTACGACACCATCTGCGAATTCACCGGGATGTCGGTGGCCG
ACTTCCTTGCCGACAAGGGCAGCCAGGTCGAGATCGTCACCGACGACATCAAACCGGGCGTGGCCATCGGTGGTACGTCG
TTCCCGACCTACTACCGCAGCATGTACCCGAAAGAAGTGATCATGACCGGCGACATGATGCTGGAGAAGGTTTACCGCGA
AGGCGACAAACTCGTCGCCGTGCTGGAGAACGAATACACCGGCGCCAAAGAGGAGCGGGTGGTGGATCAGGTGGTCGTCG
AGAACGGCGTGCGCCCGGACGAAGAAATCTACTACGCCCTCAAGGACGGCTCGCGTAACAAGGGCCAGATCGATGTCGAG
GCACTGTTTGCGATCCAGCCACAACCTTCGTTGAGCAGCACCGGCGACGGTTACTTGCTGTTCCGCATCGGTGACTGCGT
GGCGCAGCGTAATACTCACGCTGCGATCTATGACGCCCTGCGGTTGTGCAAAGACTTCTAA

Upstream 100 bases:

>100_bases
CCAGATCCGCACCGTGGCCGAGCAGGTTTACGGTGGCTCCGAAGAAGGCCACGACGACGGCCTGTTCACCGTCAAGCCGT
TGTAAGTCGAGGAACCCGCC

Downstream 100 bases:

>100_bases
AGGCTTGCACATGACCCTGTGGGAGTGGGCTTGCCCGCGAATGCATGCTGTCAGTCACCGCTGCATCGACTGACCCGGCG
CTTTCGCGAGCAAGCTCGCT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MAFEAMFQPIQIGKLTIRNRVLSTAHAEVYATDGGMTTERYVKYYEEKAKGGIGLAICGGSSVVAIDSPQQWWSSVNLST
DRIIPHFQNLADAMHKHGAKIMIQITHMGRRSRWDGFHWPTLMSPSGIREPVHRATCKTIEPEEIWRVIGNYAQAARRAK
AGGLDGVELSAVHQHMIDQFWSPRVNKRTDEWGGTFEGRMKFGLEVLKAVRAEVGDDFCVGMRICGDEFHPDGLSHEDMK
QIAKYYDDTGMLDFIGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGIKEVVKVPVLHAQNIKDPNQATRILEGGYVDM
VGMTRAHIADPHLIAKIKMGQIDQIKQCVGANYCIDRQYQGLDVLCIQNAATSREYMGVPHIIEKSTGPKRKVVIVGAGP
AGMEAARVAAERGHDVTLFEKKEFIGGQITTASKAPQRDQIAGITRWFQLELARLKVDLRLGTAADADTIMDLRPDIVVL
AVGGHPYLEQNEHWGAAEGLVVSSWDVLDGKVAPGKNVLVYDTICEFTGMSVADFLADKGSQVEIVTDDIKPGVAIGGTS
FPTYYRSMYPKEVIMTGDMMLEKVYREGDKLVAVLENEYTGAKEERVVDQVVVENGVRPDEEIYYALKDGSRNKGQIDVE
ALFAIQPQPSLSSTGDGYLLFRIGDCVAQRNTHAAIYDALRLCKDF

Sequences:

>Translated_686_residues
MAFEAMFQPIQIGKLTIRNRVLSTAHAEVYATDGGMTTERYVKYYEEKAKGGIGLAICGGSSVVAIDSPQQWWSSVNLST
DRIIPHFQNLADAMHKHGAKIMIQITHMGRRSRWDGFHWPTLMSPSGIREPVHRATCKTIEPEEIWRVIGNYAQAARRAK
AGGLDGVELSAVHQHMIDQFWSPRVNKRTDEWGGTFEGRMKFGLEVLKAVRAEVGDDFCVGMRICGDEFHPDGLSHEDMK
QIAKYYDDTGMLDFIGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGIKEVVKVPVLHAQNIKDPNQATRILEGGYVDM
VGMTRAHIADPHLIAKIKMGQIDQIKQCVGANYCIDRQYQGLDVLCIQNAATSREYMGVPHIIEKSTGPKRKVVIVGAGP
AGMEAARVAAERGHDVTLFEKKEFIGGQITTASKAPQRDQIAGITRWFQLELARLKVDLRLGTAADADTIMDLRPDIVVL
AVGGHPYLEQNEHWGAAEGLVVSSWDVLDGKVAPGKNVLVYDTICEFTGMSVADFLADKGSQVEIVTDDIKPGVAIGGTS
FPTYYRSMYPKEVIMTGDMMLEKVYREGDKLVAVLENEYTGAKEERVVDQVVVENGVRPDEEIYYALKDGSRNKGQIDVE
ALFAIQPQPSLSSTGDGYLLFRIGDCVAQRNTHAAIYDALRLCKDF
>Mature_685_residues
AFEAMFQPIQIGKLTIRNRVLSTAHAEVYATDGGMTTERYVKYYEEKAKGGIGLAICGGSSVVAIDSPQQWWSSVNLSTD
RIIPHFQNLADAMHKHGAKIMIQITHMGRRSRWDGFHWPTLMSPSGIREPVHRATCKTIEPEEIWRVIGNYAQAARRAKA
GGLDGVELSAVHQHMIDQFWSPRVNKRTDEWGGTFEGRMKFGLEVLKAVRAEVGDDFCVGMRICGDEFHPDGLSHEDMKQ
IAKYYDDTGMLDFIGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGIKEVVKVPVLHAQNIKDPNQATRILEGGYVDMV
GMTRAHIADPHLIAKIKMGQIDQIKQCVGANYCIDRQYQGLDVLCIQNAATSREYMGVPHIIEKSTGPKRKVVIVGAGPA
GMEAARVAAERGHDVTLFEKKEFIGGQITTASKAPQRDQIAGITRWFQLELARLKVDLRLGTAADADTIMDLRPDIVVLA
VGGHPYLEQNEHWGAAEGLVVSSWDVLDGKVAPGKNVLVYDTICEFTGMSVADFLADKGSQVEIVTDDIKPGVAIGGTSF
PTYYRSMYPKEVIMTGDMMLEKVYREGDKLVAVLENEYTGAKEERVVDQVVVENGVRPDEEIYYALKDGSRNKGQIDVEA
LFAIQPQPSLSSTGDGYLLFRIGDCVAQRNTHAAIYDALRLCKDF

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=674, Percent_Identity=28.486646884273, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1787939, Length=370, Percent_Identity=24.3243243243243, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=367, Percent_Identity=27.5204359673025, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=393, Percent_Identity=24.1730279898219, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559804, Length=273, Percent_Identity=28.9377289377289, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=364, Percent_Identity=26.3736263736264, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=357, Percent_Identity=25.4901960784314, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=385, Percent_Identity=24.1558441558442, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=352, Percent_Identity=23.8636363636364, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321973, Length=241, Percent_Identity=27.3858921161826, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=241, Percent_Identity=26.9709543568465, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 75761; Mature: 75629

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEAMFQPIQIGKLTIRNRVLSTAHAEVYATDGGMTTERYVKYYEEKAKGGIGLAICGG
CCCHHHCCCHHHCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECC
SSVVAIDSPQQWWSSVNLSTDRIIPHFQNLADAMHKHGAKIMIQITHMGRRSRWDGFHWP
CEEEEECCHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
TLMSPSGIREPVHRATCKTIEPEEIWRVIGNYAQAARRAKAGGLDGVELSAVHQHMIDQF
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
WSPRVNKRTDEWGGTFEGRMKFGLEVLKAVRAEVGDDFCVGMRICGDEFHPDGLSHEDMK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHEEECCCCCCCCCCHHHHH
QIAKYYDDTGMLDFIGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGIKEVVKVPVLHA
HHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
QNIKDPNQATRILEGGYVDMVGMTRAHIADPHLIAKIKMGQIDQIKQCVGANYCIDRQYQ
CCCCCHHHHHHHHCCCCEEEECCCHHHCCCCCEEEEECCCCHHHHHHHHCCCCEECCCCC
GLDVLCIQNAATSREYMGVPHIIEKSTGPKRKVVIVGAGPAGMEAARVAAERGHDVTLFE
CEEEEEECCCCCCHHHCCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEE
KKEFIGGQITTASKAPQRDQIAGITRWFQLELARLKVDLRLGTAADADTIMDLRPDIVVL
CHHHCCCEEEECCCCCCHHHHHHHHHHHHEEEEHEEEEEEECCCCCCHHHHCCCCCEEEE
AVGGHPYLEQNEHWGAAEGLVVSSWDVLDGKVAPGKNVLVYDTICEFTGMSVADFLADKG
EECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECHHHHCCCHHHHHHHCCC
SQVEIVTDDIKPGVAIGGTSFPTYYRSMYPKEVIMTGDMMLEKVYREGDKLVAVLENEYT
CEEEEEECCCCCCEEECCCCCHHHHHHCCCCHHEEEHHHHHHHHHHCCCEEEEEEECCCC
GAKEERVVDQVVVENGVRPDEEIYYALKDGSRNKGQIDVEALFAIQPQPSLSSTGDGYLL
CCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEE
FRIGDCVAQRNTHAAIYDALRLCKDF
EEECHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFEAMFQPIQIGKLTIRNRVLSTAHAEVYATDGGMTTERYVKYYEEKAKGGIGLAICGG
CCHHHCCCHHHCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECC
SSVVAIDSPQQWWSSVNLSTDRIIPHFQNLADAMHKHGAKIMIQITHMGRRSRWDGFHWP
CEEEEECCHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
TLMSPSGIREPVHRATCKTIEPEEIWRVIGNYAQAARRAKAGGLDGVELSAVHQHMIDQF
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
WSPRVNKRTDEWGGTFEGRMKFGLEVLKAVRAEVGDDFCVGMRICGDEFHPDGLSHEDMK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHEEECCCCCCCCCCHHHHH
QIAKYYDDTGMLDFIGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGIKEVVKVPVLHA
HHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
QNIKDPNQATRILEGGYVDMVGMTRAHIADPHLIAKIKMGQIDQIKQCVGANYCIDRQYQ
CCCCCHHHHHHHHCCCCEEEECCCHHHCCCCCEEEEECCCCHHHHHHHHCCCCEECCCCC
GLDVLCIQNAATSREYMGVPHIIEKSTGPKRKVVIVGAGPAGMEAARVAAERGHDVTLFE
CEEEEEECCCCCCHHHCCCCHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEE
KKEFIGGQITTASKAPQRDQIAGITRWFQLELARLKVDLRLGTAADADTIMDLRPDIVVL
CHHHCCCEEEECCCCCCHHHHHHHHHHHHEEEEHEEEEEEECCCCCCHHHHCCCCCEEEE
AVGGHPYLEQNEHWGAAEGLVVSSWDVLDGKVAPGKNVLVYDTICEFTGMSVADFLADKG
EECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECHHHHCCCHHHHHHHCCC
SQVEIVTDDIKPGVAIGGTSFPTYYRSMYPKEVIMTGDMMLEKVYREGDKLVAVLENEYT
CEEEEEECCCCCCEEECCCCCHHHHHHCCCCHHEEEHHHHHHHHHHCCCEEEEEEECCCC
GAKEERVVDQVVVENGVRPDEEIYYALKDGSRNKGQIDVEALFAIQPQPSLSSTGDGYLL
CCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCCEEE
FRIGDCVAQRNTHAAIYDALRLCKDF
EEECHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]