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The map label for this gene is purU [H]

Identifier: 77461424

GI number: 77461424

Start: 5850776

End: 5851633

Strand: Reverse

Name: purU [H]

Synonym: Pfl01_5203

Alternate gene names: 77461424

Gene position: 5851633-5850776 (Counterclockwise)

Preceding gene: 77461425

Following gene: 77461423

Centisome position: 90.89

GC content: 61.89

Gene sequence:

>858_bases
ATGAGCCGCGCCCCGGATACATGGATTCTGACCGCCGACTGCCCCAGCGTTCTCGGCACCGTGGACGCGGTGACGCGTTT
TCTGTTCGAGCAAGGTTGCTACGTGACCGAGCATCACTCGTTCGATGACCGGCTCTCGGGCCGCTTCTTTATTCGGGTGG
AGTTCCGTCAGCCCGACGGTTTTGACGAACAGTCCTTCCGCGCCGGCCTCGCCGAGCGTGGTCAGGCGTTCGGCATGATC
TTCGAGCTGACTGCGCCGAACTACCGGCCAAAAGTGGTGATCATGGTCTCCAAGGCCGATCACTGCCTCAACGACTTGCT
CTATCGCCAGCGCATCGGCCAGTTGTCGATGGACGTGGCGGCGGTGGTGTCCAACCATCCGGACCTCAAGCCGCTGGCCG
ACTGGCACCAGATTCCCTACTACCATTTCCCCCTCGACCCCAACGACAAACCGGCCCAGGAGCGGCAGGTGTGGCAGGTG
ATCGAAGAGGCCGGCGCTGAACTGGTGATCCTCGCCCGCTACATGCAGGTGCTGTCGCCGGAACTGTGCCGCAAGCTCGA
TGGCAAGGCGATCAACATTCACCACTCGCTGCTGCCGGGCTTCAAGGGCGCCAAGCCGTATCACCAGGCCTACAACAAGG
GCGTGAAACTGGTAGGCGCCACCGCGCACTACATCAATAACGACCTGGACGAGGGGCCGATCATCGCCCAAGGCGTCGAG
GCGGTGGATCACAGTCATTACCCCGAAGACCTGATTGCCAAGGGCCGCGACATCGAAGGCCTGACCCTGGCCCGGGCCGT
GGGGTATCACATTGAGCGGAGGGTGTTTTTGAACGCCAATCGCACCGTCGTTCTTTGA

Upstream 100 bases:

>100_bases
CTGCTGATTCGCCGCAGCTTCTCGGATTACTGGTGGTTGTGGTTGCAGGATGCGTCCGCCGAATACGGCCTTAGCGTTCA
GGCCTGATCAGGAGAAAACG

Downstream 100 bases:

>100_bases
GTCACAAGCAACGCAAAACCAACAAGCAATAACCCGAGCACTTAACGCGCTACCTGCCTGGGCGGCGCGGTTCCATAAAA
ACAACAGCGAGGTGAAAGCA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSRAPDTWILTADCPSVLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPDGFDEQSFRAGLAERGQAFGMI
FELTAPNYRPKVVIMVSKADHCLNDLLYRQRIGQLSMDVAAVVSNHPDLKPLADWHQIPYYHFPLDPNDKPAQERQVWQV
IEEAGAELVILARYMQVLSPELCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
AVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL

Sequences:

>Translated_285_residues
MSRAPDTWILTADCPSVLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPDGFDEQSFRAGLAERGQAFGMI
FELTAPNYRPKVVIMVSKADHCLNDLLYRQRIGQLSMDVAAVVSNHPDLKPLADWHQIPYYHFPLDPNDKPAQERQVWQV
IEEAGAELVILARYMQVLSPELCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
AVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL
>Mature_284_residues
SRAPDTWILTADCPSVLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPDGFDEQSFRAGLAERGQAFGMIF
ELTAPNYRPKVVIMVSKADHCLNDLLYRQRIGQLSMDVAAVVSNHPDLKPLADWHQIPYYHFPLDPNDKPAQERQVWQVI
EEAGAELVILARYMQVLSPELCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVEA
VDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=156, Percent_Identity=32.6923076923077, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI209869995, Length=156, Percent_Identity=32.6923076923077, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI209869993, Length=156, Percent_Identity=32.6923076923077, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=38.9891696750903, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=24.8704663212435, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=156, Percent_Identity=37.1794871794872, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32188; Mature: 32057

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAPDTWILTADCPSVLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPDG
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEEECCCC
FDEQSFRAGLAERGQAFGMIFELTAPNYRPKVVIMVSKADHCLNDLLYRQRIGQLSMDVA
CCHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
AVVSNHPDLKPLADWHQIPYYHFPLDPNDKPAQERQVWQVIEEAGAELVILARYMQVLSP
HHHCCCCCCCCCHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCH
ELCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
HHHHHCCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHH
AVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL
HHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHEEEEEEECCCEEEC
>Mature Secondary Structure 
SRAPDTWILTADCPSVLGTVDAVTRFLFEQGCYVTEHHSFDDRLSGRFFIRVEFRQPDG
CCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEEECCCC
FDEQSFRAGLAERGQAFGMIFELTAPNYRPKVVIMVSKADHCLNDLLYRQRIGQLSMDVA
CCHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHH
AVVSNHPDLKPLADWHQIPYYHFPLDPNDKPAQERQVWQVIEEAGAELVILARYMQVLSP
HHHCCCCCCCCCHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCH
ELCRKLDGKAINIHHSLLPGFKGAKPYHQAYNKGVKLVGATAHYINNDLDEGPIIAQGVE
HHHHHCCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHH
AVDHSHYPEDLIAKGRDIEGLTLARAVGYHIERRVFLNANRTVVL
HHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHEEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]