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The map label for this gene is yafV [C]

Identifier: 77461376

GI number: 77461376

Start: 5796196

End: 5796990

Strand: Direct

Name: yafV [C]

Synonym: Pfl01_5155

Alternate gene names: 77461376

Gene position: 5796196-5796990 (Clockwise)

Preceding gene: 77461375

Following gene: 77461377

Centisome position: 90.03

GC content: 61.13

Gene sequence:

>795_bases
ATGCGTGTAGCCCTTTACCAATGTCCACCGCTGCCACTGGAACCCGCCGCCAACCTTCAGCGCCTGCACCAACTGGCGAT
GGAGGCCAAAGGCGCCGACCTGCTGGTGGTGCCGGAGATGTTCCTGACCGGCTACAACATCGGCAAGGAGGCGGTCGCGA
CATTGGCCGAGGTCTACAACGGTGAGTGGGCGCAGCAAGTCGGACGGATCGCCAAGGCTGCCGGTCTGGCGATCCTTTAC
GGTTACCCTGAGCGCACCGCCGAAGGGCAGATCTATAACGCCGTGCAGTTGATCGATTCGAGCGGCGAGCGCCTGTGCAA
TTACCGCAAGACGCATCTGTTCGGCGATCTGGATCGTTCGATGTTCAGCCCCGGCGATGGCGAGTTTCCCATCGTCGAGC
TCAACGGCTGGAAGCTCGGTTTCCTGATCTGCTACGACCTGGAGTTCCCGGAGAATGCCCGGCGCCTGGCCCTTGAGGGC
GCCGAGCTGATTCTGGTGCCGACCGCCAACATGATTCCTTTCGATTTCATTGCCGATGTTTCCGTGCGCTCCCGTGCCTT
CGAAAACCAGTGCTACGTGGCTTACGCCAACTACTGCGGCCACGAAGGCGACATCCATTACTGCGGACAAAGCAGCATTG
CCGCGCCGGATGGCAGCCGCATTGCTCAGGCCGGGCTGGATGAAGCCTTGATCGTCGGCGAGCTGGATCGCCAGTTGATG
GTCGACTCGCGCAGCGCCAACCGTTACTTCAACGACCGCCGTCCCGAACTTTACGACGCGCTCAACAAGCGCTAA

Upstream 100 bases:

>100_bases
GAAGCACTTCGGCGGGTCCACACATAAAGAGAACCCTGGCCCGGGTGATGTGTTCAACGACATCGGCCCGATCGCCCTGC
CCGAGTAAGAGGAATCTTCA

Downstream 100 bases:

>100_bases
TCCGCTAGCATTGGCACTTCACTGTTCTGGAAGTGCCCATGCCTGCGCCGACCCATCCCCGTCCCCACACCGAAACCCTG
GCCAACGGCTTGCGCGTGAC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRVALYQCPPLPLEPAANLQRLHQLAMEAKGADLLVVPEMFLTGYNIGKEAVATLAEVYNGEWAQQVGRIAKAAGLAILY
GYPERTAEGQIYNAVQLIDSSGERLCNYRKTHLFGDLDRSMFSPGDGEFPIVELNGWKLGFLICYDLEFPENARRLALEG
AELILVPTANMIPFDFIADVSVRSRAFENQCYVAYANYCGHEGDIHYCGQSSIAAPDGSRIAQAGLDEALIVGELDRQLM
VDSRSANRYFNDRRPELYDALNKR

Sequences:

>Translated_264_residues
MRVALYQCPPLPLEPAANLQRLHQLAMEAKGADLLVVPEMFLTGYNIGKEAVATLAEVYNGEWAQQVGRIAKAAGLAILY
GYPERTAEGQIYNAVQLIDSSGERLCNYRKTHLFGDLDRSMFSPGDGEFPIVELNGWKLGFLICYDLEFPENARRLALEG
AELILVPTANMIPFDFIADVSVRSRAFENQCYVAYANYCGHEGDIHYCGQSSIAAPDGSRIAQAGLDEALIVGELDRQLM
VDSRSANRYFNDRRPELYDALNKR
>Mature_264_residues
MRVALYQCPPLPLEPAANLQRLHQLAMEAKGADLLVVPEMFLTGYNIGKEAVATLAEVYNGEWAQQVGRIAKAAGLAILY
GYPERTAEGQIYNAVQLIDSSGERLCNYRKTHLFGDLDRSMFSPGDGEFPIVELNGWKLGFLICYDLEFPENARRLALEG
AELILVPTANMIPFDFIADVSVRSRAFENQCYVAYANYCGHEGDIHYCGQSSIAAPDGSRIAQAGLDEALIVGELDRQLM
VDSRSANRYFNDRRPELYDALNKR

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=261, Percent_Identity=30.6513409961686, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI5031947, Length=265, Percent_Identity=32.0754716981132, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI297632350, Length=265, Percent_Identity=32.0754716981132, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI297632348, Length=265, Percent_Identity=32.0754716981132, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1786412, Length=265, Percent_Identity=29.811320754717, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556280, Length=244, Percent_Identity=28.6885245901639, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17533173, Length=224, Percent_Identity=27.2321428571429, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323383, Length=283, Percent_Identity=29.3286219081272, Blast_Score=89, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6322335, Length=201, Percent_Identity=30.8457711442786, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355835, Length=276, Percent_Identity=28.2608695652174, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21358471, Length=206, Percent_Identity=30.0970873786408, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17933642, Length=264, Percent_Identity=29.5454545454545, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29378; Mature: 29378

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALYQCPPLPLEPAANLQRLHQLAMEAKGADLLVVPEMFLTGYNIGKEAVATLAEVYN
CEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCCHHHHHHHHHHHC
GEWAQQVGRIAKAAGLAILYGYPERTAEGQIYNAVQLIDSSGERLCNYRKTHLFGDLDRS
CHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MFSPGDGEFPIVELNGWKLGFLICYDLEFPENARRLALEGAELILVPTANMIPFDFIADV
HCCCCCCCEEEEEECCEEEEEEEEEECCCCCCHHEEEECCCCEEEEECCCCCCHHHHHCC
SVRSRAFENQCYVAYANYCGHEGDIHYCGQSSIAAPDGSRIAQAGLDEALIVGELDRQLM
HHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCEEEEEHHCHHHH
VDSRSANRYFNDRRPELYDALNKR
HCCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MRVALYQCPPLPLEPAANLQRLHQLAMEAKGADLLVVPEMFLTGYNIGKEAVATLAEVYN
CEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCCHHHHHHHHHHHC
GEWAQQVGRIAKAAGLAILYGYPERTAEGQIYNAVQLIDSSGERLCNYRKTHLFGDLDRS
CHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MFSPGDGEFPIVELNGWKLGFLICYDLEFPENARRLALEGAELILVPTANMIPFDFIADV
HCCCCCCCEEEEEECCEEEEEEEEEECCCCCCHHEEEECCCCEEEEECCCCCCHHHHHCC
SVRSRAFENQCYVAYANYCGHEGDIHYCGQSSIAAPDGSRIAQAGLDEALIVGELDRQLM
HHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHCCCCEEEEEHHCHHHH
VDSRSANRYFNDRRPELYDALNKR
HCCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8526497 [H]