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The map label for this gene is yjfC [H]

Identifier: 77461269

GI number: 77461269

Start: 5683378

End: 5684535

Strand: Direct

Name: yjfC [H]

Synonym: Pfl01_5048

Alternate gene names: 77461269

Gene position: 5683378-5684535 (Clockwise)

Preceding gene: 77461268

Following gene: 77461272

Centisome position: 88.27

GC content: 59.84

Gene sequence:

>1158_bases
ATGAAGAAGATCCACTGCGCCGAGCGCCACGACTGGAAGCAGACCGCCGAAAGCCTCGGCTTTCTGTTTCATACCATCGA
CGACGAGCCGTACTGGGACGAGCGCGCCTATTACCAGTTCACGCTCGCACAGATCGAAAACGATCTGGAGGATCCAACCA
CCGAGCTGCACGAGATGTGCATGGACCTGGTCGACCGCGTCGTGCACAGCGAAGAACTGCTGGGTCGCCTGAGCATTCCG
GCGTCGTACTACGACATGATCCGAACGTCCTGGCTGGAAGGTCATCCGCATCTGTACGGGCGCATGGACTTCTCCTACAG
CGGCAATGGCCCGGCGAAGCTGCTCGAGCTCAACTACGACACGCCCACGAGTCTTTACGAAGCGTCGGCGTTCCAGTGGG
GCTGGCTGGAGCAATGCATCGAGCGCGGGATGCTGCCGCGCCATGCCGACCAGTTCAACAGCATCGACACCCGGCTGCAC
GAAGCCTTTGCCCACCTGAAACTCACGCGGCCGTTTTACTTCGCCTCGATGAAAGACTCGGTCGAAGACAAGGGCACCAC
GGATTACCTGCGGCTGATCGCAGAAAAGGTCGGCATCGAATCGCGGCACATCGATATCGAAGACATTGGCCTGAGCGCTG
ACGGTCGTTTCGTGGATCTCGAGGATCGCTGGATCCCGCACCTGTTCAAACTGCACGCCTGGGAGTTCATCTTCCACGAA
CCGTTCGGCGCGGCGATCGCCGAATGCGACACCCAGTTTTTCGAACCGGCGTGGAAATCCATTCTCTCCAACAAGGGCGC
CTTGCCGTTGCTTTGGGAACTGCACAAGGGTCATCCGAACCTGCTCGCCGCACATCTCGATCCGAATCCGGGCAGCGCGG
TGCCCAAGGGCTGGGTACGCAAGCCGTACTTCTCCCGGGAAGGTGCCAACATCGAGTTGCAGACAGCCGAAGGCCTGATC
GTCAAAGAGGATGGGCCCTACACCGACGCGCCCTTCATCCTTCAGGAACTTGCCCCGTTGCCGAAGTTCGACGACAGCTA
CACGCTGATCGGCTCCTGGGTGATCGGCGATCAGGCGGCGGGGATTGGCGTGCGCGAAGACAACAGCCTGATCACCAAGG
ATTCGAGTCGGTTCCTGCCGCATCTGATTCTTGACTGA

Upstream 100 bases:

>100_bases
AACCGGTCAACGTCGCTGCGTCCACCTCCCGTGGCGGCTTCGGCAGCCAGTCCAGCGCACGCAGTGGTTGGGGCGGCTGG
GGCCGTTCGAGCAGCTGACC

Downstream 100 bases:

>100_bases
AAACCCGCGCACATGAAAAAGGCCCGTCGCATTCAGCGACGGGCCTTTTTTATTTAGCGCGGATACAGCAGCGAATCAGA
ACGGAATGTCGTCATCGAAG

Product: glutathionylspermidine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKKIHCAERHDWKQTAESLGFLFHTIDDEPYWDERAYYQFTLAQIENDLEDPTTELHEMCMDLVDRVVHSEELLGRLSIP
ASYYDMIRTSWLEGHPHLYGRMDFSYSGNGPAKLLELNYDTPTSLYEASAFQWGWLEQCIERGMLPRHADQFNSIDTRLH
EAFAHLKLTRPFYFASMKDSVEDKGTTDYLRLIAEKVGIESRHIDIEDIGLSADGRFVDLEDRWIPHLFKLHAWEFIFHE
PFGAAIAECDTQFFEPAWKSILSNKGALPLLWELHKGHPNLLAAHLDPNPGSAVPKGWVRKPYFSREGANIELQTAEGLI
VKEDGPYTDAPFILQELAPLPKFDDSYTLIGSWVIGDQAAGIGVREDNSLITKDSSRFLPHLILD

Sequences:

>Translated_385_residues
MKKIHCAERHDWKQTAESLGFLFHTIDDEPYWDERAYYQFTLAQIENDLEDPTTELHEMCMDLVDRVVHSEELLGRLSIP
ASYYDMIRTSWLEGHPHLYGRMDFSYSGNGPAKLLELNYDTPTSLYEASAFQWGWLEQCIERGMLPRHADQFNSIDTRLH
EAFAHLKLTRPFYFASMKDSVEDKGTTDYLRLIAEKVGIESRHIDIEDIGLSADGRFVDLEDRWIPHLFKLHAWEFIFHE
PFGAAIAECDTQFFEPAWKSILSNKGALPLLWELHKGHPNLLAAHLDPNPGSAVPKGWVRKPYFSREGANIELQTAEGLI
VKEDGPYTDAPFILQELAPLPKFDDSYTLIGSWVIGDQAAGIGVREDNSLITKDSSRFLPHLILD
>Mature_385_residues
MKKIHCAERHDWKQTAESLGFLFHTIDDEPYWDERAYYQFTLAQIENDLEDPTTELHEMCMDLVDRVVHSEELLGRLSIP
ASYYDMIRTSWLEGHPHLYGRMDFSYSGNGPAKLLELNYDTPTSLYEASAFQWGWLEQCIERGMLPRHADQFNSIDTRLH
EAFAHLKLTRPFYFASMKDSVEDKGTTDYLRLIAEKVGIESRHIDIEDIGLSADGRFVDLEDRWIPHLFKLHAWEFIFHE
PFGAAIAECDTQFFEPAWKSILSNKGALPLLWELHKGHPNLLAAHLDPNPGSAVPKGWVRKPYFSREGANIELQTAEGLI
VKEDGPYTDAPFILQELAPLPKFDDSYTLIGSWVIGDQAAGIGVREDNSLITKDSSRFLPHLILD

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli ygiC [H]

Homologues:

Organism=Escherichia coli, GI1790629, Length=385, Percent_Identity=50.3896103896104, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1789416, Length=388, Percent_Identity=46.9072164948454, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789361, Length=390, Percent_Identity=27.6923076923077, Blast_Score=127, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: NA

Molecular weight: Translated: 44166; Mature: 44166

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIHCAERHDWKQTAESLGFLFHTIDDEPYWDERAYYQFTLAQIENDLEDPTTELHEMC
CCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCHHHEEEHHHHHHHCCCHHHHHHHHH
MDLVDRVVHSEELLGRLSIPASYYDMIRTSWLEGHPHLYGRMDFSYSGNGPAKLLELNYD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCC
TPTSLYEASAFQWGWLEQCIERGMLPRHADQFNSIDTRLHEAFAHLKLTRPFYFASMKDS
CCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCEEECCCHHH
VEDKGTTDYLRLIAEKVGIESRHIDIEDIGLSADGRFVDLEDRWIPHLFKLHAWEFIFHE
HCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCC
PFGAAIAECDTQFFEPAWKSILSNKGALPLLWELHKGHPNLLAAHLDPNPGSAVPKGWVR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHEECCCCCCEEEEEECCCCCCCCCCCCCC
KPYFSREGANIELQTAEGLIVKEDGPYTDAPFILQELAPLPKFDDSYTLIGSWVIGDQAA
CCCCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEECCCCC
GIGVREDNSLITKDSSRFLPHLILD
CCCEECCCCEEECCCHHCCCHHHCC
>Mature Secondary Structure
MKKIHCAERHDWKQTAESLGFLFHTIDDEPYWDERAYYQFTLAQIENDLEDPTTELHEMC
CCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCHHHEEEHHHHHHHCCCHHHHHHHHH
MDLVDRVVHSEELLGRLSIPASYYDMIRTSWLEGHPHLYGRMDFSYSGNGPAKLLELNYD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCC
TPTSLYEASAFQWGWLEQCIERGMLPRHADQFNSIDTRLHEAFAHLKLTRPFYFASMKDS
CCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEECCCEEECCCHHH
VEDKGTTDYLRLIAEKVGIESRHIDIEDIGLSADGRFVDLEDRWIPHLFKLHAWEFIFHE
HCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCC
PFGAAIAECDTQFFEPAWKSILSNKGALPLLWELHKGHPNLLAAHLDPNPGSAVPKGWVR
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHEECCCCCCEEEEEECCCCCCCCCCCCCC
KPYFSREGANIELQTAEGLIVKEDGPYTDAPFILQELAPLPKFDDSYTLIGSWVIGDQAA
CCCCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEECCCCC
GIGVREDNSLITKDSSRFLPHLILD
CCCEECCCCEEECCCHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7961409 [H]