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The map label for this gene is mpl [H]

Identifier: 77461208

GI number: 77461208

Start: 5616357

End: 5617706

Strand: Reverse

Name: mpl [H]

Synonym: Pfl01_4987

Alternate gene names: 77461208

Gene position: 5617706-5616357 (Counterclockwise)

Preceding gene: 77461209

Following gene: 77461207

Centisome position: 87.25

GC content: 62.59

Gene sequence:

>1350_bases
ATGCACATTCATATTCTGGGTATCTGCGGGACTTTCATGGGCTCGATGGCGGTTCTGGCCAAAGAGCTGGGCCATCACGT
GACTGGCTCCGATGCCAACGTCTATCCGCCGATGAGCACCCAGCTCGAAGCCCAGGGCATTCAGTTGACCCAGGGCTACG
ACCCGGCGCAGCTTGATCCGGCACCGGATCTGGTGGTGATCGGCAACGCCATGTCCCGCGGCAATCCGGCAGTGGAATAT
GTGCTGAACAAAGGCCTGCCGTACGTTTCCGGCCCGCAATGGCTGGCCGACCATGTGCTGCAAGGTCGCTGGGTGCTGGC
GGTTGCCGGTACGCACGGCAAGACCACCACCAGCAGCATGCTCGCCTGGGTACTGGAACACGCCGGCATGAGCCCGGGCT
TCCTGATCGGCGGCGTGCCGCAGAACTTCTCGGTGTCGGCACGTCTGGGTGGTACGCCGTTCTTCGTGATCGAGGCTGAC
GAATACGACAGCGCGTTCTTCGACAAGCGATCGAAGTTTGTCCACTACCGTCCGCGCACGGCGATCCTCAACAACCTTGA
GTTCGATCATGCCGACATCTTCCCTGATCTGCCAGCCATCGAGCGGCAGTTCCACCACTTGGTGCGCACCATCCCGAGCG
AAGGCCTGGTGATTCACCCGACCACCGAACCGGCCTTGCAGCGTGTGATCGAGATGGGCTGCTGGACCCCGGTGCAGACC
ACCGGTGCCGGCGGTCAGTGGCAGGTCAAGTTGCTGAAGGACGACGGCTCGGCGTTTGAAGTGATGTTCGAAGGCGTGTC
CCAAGGCGTGGTCGAGTGGGAGTTGACCGGTCAGCACAACGTTGCCAACGCCCTGGCCACTCTGGCCGCGGCGCGTCATG
TCGGCGTTGTACCGTCGATGGGCATCGCCGGGTTGAGCGCGTTCAAGAGCGTGAAACGCCGGATGGAGAAAGTCGCGGAA
GTTCGGGGAATCACCATCTACGATGACTTCGCCCACCACCCGACCGCGATTGCCACCACCCTTGATGGCCTGCGCAAACG
TATCGGCGATGCGCCGCTGATCGCGATCATCGAGCCGCGCTCCAACTCGATGAAGCTCGGCGCCCACCGTGACGGTCTGC
CGGACAGCGTGGTCGATGCCGATCAAGTGATCTGGTACGCCCCGGCCAACCTCGGTTGGGATCTGGCAGGCACCGCCGCG
TTGTGCACCGTGCCGTCGATCGTCAGTGATTCGCTGGAAGGCATCATTGAGCGCGTGAAGAGCCAGGCTCAGCCCGGCAC
TCACGTGGTGATCATGAGCAACGGCGGTTTCGGCGGCCTGCACGGCAAACTCGCCGAGGCGTTGAAATGA

Upstream 100 bases:

>100_bases
CGGAGCGTGGGAACGATCAAGGCCTCCTCCCTCAATCAGCTAAAGAGTGCGATTTCCCCCTCCCTGCGCTACCCTGCGGC
CATGTTTTACGAGGTCGACT

Downstream 100 bases:

>100_bases
ACAACAACGGCCCAGAACGCATCACACTGGCGATGACCGGCGCGTCCGGCGCCCAGTACGGCTTGCGCCTGCTGGATTGC
CTGGTGCGCGAGGATCGCGA

Product: L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MHIHILGICGTFMGSMAVLAKELGHHVTGSDANVYPPMSTQLEAQGIQLTQGYDPAQLDPAPDLVVIGNAMSRGNPAVEY
VLNKGLPYVSGPQWLADHVLQGRWVLAVAGTHGKTTTSSMLAWVLEHAGMSPGFLIGGVPQNFSVSARLGGTPFFVIEAD
EYDSAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLPAIERQFHHLVRTIPSEGLVIHPTTEPALQRVIEMGCWTPVQT
TGAGGQWQVKLLKDDGSAFEVMFEGVSQGVVEWELTGQHNVANALATLAAARHVGVVPSMGIAGLSAFKSVKRRMEKVAE
VRGITIYDDFAHHPTAIATTLDGLRKRIGDAPLIAIIEPRSNSMKLGAHRDGLPDSVVDADQVIWYAPANLGWDLAGTAA
LCTVPSIVSDSLEGIIERVKSQAQPGTHVVIMSNGGFGGLHGKLAEALK

Sequences:

>Translated_449_residues
MHIHILGICGTFMGSMAVLAKELGHHVTGSDANVYPPMSTQLEAQGIQLTQGYDPAQLDPAPDLVVIGNAMSRGNPAVEY
VLNKGLPYVSGPQWLADHVLQGRWVLAVAGTHGKTTTSSMLAWVLEHAGMSPGFLIGGVPQNFSVSARLGGTPFFVIEAD
EYDSAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLPAIERQFHHLVRTIPSEGLVIHPTTEPALQRVIEMGCWTPVQT
TGAGGQWQVKLLKDDGSAFEVMFEGVSQGVVEWELTGQHNVANALATLAAARHVGVVPSMGIAGLSAFKSVKRRMEKVAE
VRGITIYDDFAHHPTAIATTLDGLRKRIGDAPLIAIIEPRSNSMKLGAHRDGLPDSVVDADQVIWYAPANLGWDLAGTAA
LCTVPSIVSDSLEGIIERVKSQAQPGTHVVIMSNGGFGGLHGKLAEALK
>Mature_449_residues
MHIHILGICGTFMGSMAVLAKELGHHVTGSDANVYPPMSTQLEAQGIQLTQGYDPAQLDPAPDLVVIGNAMSRGNPAVEY
VLNKGLPYVSGPQWLADHVLQGRWVLAVAGTHGKTTTSSMLAWVLEHAGMSPGFLIGGVPQNFSVSARLGGTPFFVIEAD
EYDSAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLPAIERQFHHLVRTIPSEGLVIHPTTEPALQRVIEMGCWTPVQT
TGAGGQWQVKLLKDDGSAFEVMFEGVSQGVVEWELTGQHNVANALATLAAARHVGVVPSMGIAGLSAFKSVKRRMEKVAE
VRGITIYDDFAHHPTAIATTLDGLRKRIGDAPLIAIIEPRSNSMKLGAHRDGLPDSVVDADQVIWYAPANLGWDLAGTAA
LCTVPSIVSDSLEGIIERVKSQAQPGTHVVIMSNGGFGGLHGKLAEALK

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=449, Percent_Identity=58.7973273942094, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1786279, Length=356, Percent_Identity=28.0898876404494, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48202; Mature: 48202

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGSMAVLAKELGHHVTGSDANVYPPMSTQLEAQGIQLTQGYDPAQLDP
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
APDLVVIGNAMSRGNPAVEYVLNKGLPYVSGPQWLADHVLQGRWVLAVAGTHGKTTTSSM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWVLEHAGMSPGFLIGGVPQNFSVSARLGGTPFFVIEADEYDSAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCEEEEEECCCCCHHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDLPAIERQFHHLVRTIPSEGLVIHPTTEPALQRVIEMGCWTPVQT
HHHCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCCEE
TGAGGQWQVKLLKDDGSAFEVMFEGVSQGVVEWELTGQHNVANALATLAAARHVGVVPSM
CCCCCEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
GIAGLSAFKSVKRRMEKVAEVRGITIYDDFAHHPTAIATTLDGLRKRIGDAPLIAIIEPR
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
SNSMKLGAHRDGLPDSVVDADQVIWYAPANLGWDLAGTAALCTVPSIVSDSLEGIIERVK
CCCEEECCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SQAQPGTHVVIMSNGGFGGLHGKLAEALK
HHCCCCCEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MHIHILGICGTFMGSMAVLAKELGHHVTGSDANVYPPMSTQLEAQGIQLTQGYDPAQLDP
CEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
APDLVVIGNAMSRGNPAVEYVLNKGLPYVSGPQWLADHVLQGRWVLAVAGTHGKTTTSSM
CCCEEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWVLEHAGMSPGFLIGGVPQNFSVSARLGGTPFFVIEADEYDSAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEECCCCCCCEEEEEECCCEEEEEECCCCCHHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDLPAIERQFHHLVRTIPSEGLVIHPTTEPALQRVIEMGCWTPVQT
HHHCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCCEE
TGAGGQWQVKLLKDDGSAFEVMFEGVSQGVVEWELTGQHNVANALATLAAARHVGVVPSM
CCCCCEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCC
GIAGLSAFKSVKRRMEKVAEVRGITIYDDFAHHPTAIATTLDGLRKRIGDAPLIAIIEPR
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
SNSMKLGAHRDGLPDSVVDADQVIWYAPANLGWDLAGTAALCTVPSIVSDSLEGIIERVK
CCCEEECCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SQAQPGTHVVIMSNGGFGGLHGKLAEALK
HHCCCCCEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]