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The map label for this gene is proB [H]

Identifier: 77461078

GI number: 77461078

Start: 5476666

End: 5477787

Strand: Reverse

Name: proB [H]

Synonym: Pfl01_4857

Alternate gene names: 77461078

Gene position: 5477787-5476666 (Counterclockwise)

Preceding gene: 77461079

Following gene: 77461077

Centisome position: 85.08

GC content: 63.1

Gene sequence:

>1122_bases
ATGATGCGGAGCAAGGTGACAGGTGCGCAGCGTTGGGTCGTGAAGATCGGCAGCGCTTTGCTGACAGCGGATGGCAAAGG
GCTGGATCGCACGGCAATGGGTGTCTGGGTCGAGCAGATGGTGGCTCTGCATGAGGCTGGCGTCGAGCTGGTGCTGGTGT
CCTCCGGGGCGGTGGCGGCAGGCATGAGTCGCCTGGGCTGGACTGCGCGACCCAGTGCGATGCATGAGCTCCAGGCTGCC
GCTGCAATCGGTCAGATGGGTCTGGTGCAAGCCTGGGAATCGAGCTTTGCCGAGCATGGCCGGCACACCGCGCAGATTCT
TCTGACTCACGACGACCTGTCCGATCGCAAGCGTTACCTGAACGCCCGCAGCACCTTGCGTGCGCTGGTCGAGCTGAAAG
TCATCCCAGTGATCAACGAGAACGACACCGTGGTCACTGACGAAATCCGCTTCGGCGACAACGACACGCTCGCGGCGCTG
GTGGCCAACCTGGTCGAGGCTGATCTGCTGGTGATCCTGACGGATCGCGACGGCATGTTCGACGCCGATCCGCGCAGTAA
CCCTGACGCCCAACTGATTTACGAAGCGCGCGCCGATGATCCGACACTGGATGCGGTGGCGGGCGGTACTGGCGGTGCAT
TGGGTCGTGGCGGCATGCAGACCAAGCTGCGCGCGGCGCGGCTGGCGGCACGTTCCGGGGCGCATACCATCATTGTTGGT
GGGCGTCTGGAGCGCGTGCTTGATCGTCTGAAGGCGGGCGAGCGCATCGGTACGCTGCTGTCGCCTGAGCGCGGCATGCT
CGCGGCGCGCAAGCAGTGGCTGGCCGGGCATCTGCAAACCCGTGGCACGCTGGTGCTGGATGATGGCGCGGTTTCTGCGT
TGTCCCAGGGCAACAAAAGTCTGCTGCCGGTCGGCGTCAAACTGGTTCAGGGCAGTTTCCGTCGCGGCGAAATGGTGGTT
TGCGTGGCGCCGGACGGTCGTGAGATCGCCCGCGGCCTGGCGAACTACAGTGCTCTGGAAGCACAAAAAATCATCGGTCA
GTCGTCAGATGCGATTGTCGGTTTGCTGGGTTACATGGCTGAGCCGGAACTGGTTCATCGTGACAACCTGATTCTGGTGT
AA

Upstream 100 bases:

>100_bases
GCGGCGTTTTAGTATCTGGAAATCGATTGTTGGTCACGAAGAATCACGAATAACGTCACGGGCAGAGCCAGGTCGCGCTG
CCCTCAAGCTAAGGTTGAAG

Downstream 100 bases:

>100_bases
GGAAAACGCGATGTGCATGGCAAAAGGATTGTTGGGCTTGCTGATGGCGCTGCCATTGCTGGCCTCGGCCGAGGAAATCG
GTCAGGTGTCGACGGTGTTC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MMRSKVTGAQRWVVKIGSALLTADGKGLDRTAMGVWVEQMVALHEAGVELVLVSSGAVAAGMSRLGWTARPSAMHELQAA
AAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLNARSTLRALVELKVIPVINENDTVVTDEIRFGDNDTLAAL
VANLVEADLLVILTDRDGMFDADPRSNPDAQLIYEARADDPTLDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVG
GRLERVLDRLKAGERIGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVSALSQGNKSLLPVGVKLVQGSFRRGEMVV
CVAPDGREIARGLANYSALEAQKIIGQSSDAIVGLLGYMAEPELVHRDNLILV

Sequences:

>Translated_373_residues
MMRSKVTGAQRWVVKIGSALLTADGKGLDRTAMGVWVEQMVALHEAGVELVLVSSGAVAAGMSRLGWTARPSAMHELQAA
AAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLNARSTLRALVELKVIPVINENDTVVTDEIRFGDNDTLAAL
VANLVEADLLVILTDRDGMFDADPRSNPDAQLIYEARADDPTLDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVG
GRLERVLDRLKAGERIGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVSALSQGNKSLLPVGVKLVQGSFRRGEMVV
CVAPDGREIARGLANYSALEAQKIIGQSSDAIVGLLGYMAEPELVHRDNLILV
>Mature_373_residues
MMRSKVTGAQRWVVKIGSALLTADGKGLDRTAMGVWVEQMVALHEAGVELVLVSSGAVAAGMSRLGWTARPSAMHELQAA
AAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLNARSTLRALVELKVIPVINENDTVVTDEIRFGDNDTLAAL
VANLVEADLLVILTDRDGMFDADPRSNPDAQLIYEARADDPTLDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVG
GRLERVLDRLKAGERIGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVSALSQGNKSLLPVGVKLVQGSFRRGEMVV
CVAPDGREIARGLANYSALEAQKIIGQSSDAIVGLLGYMAEPELVHRDNLILV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=295, Percent_Identity=34.5762711864407, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI62912457, Length=293, Percent_Identity=34.8122866894198, Blast_Score=138, Evalue=7e-33,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=43.7158469945355, Blast_Score=293, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=34.0501792114695, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=34.0501792114695, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=411, Percent_Identity=35.7664233576642, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=421, Percent_Identity=33.2541567695962, Blast_Score=204, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39754; Mature: 39754

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRSKVTGAQRWVVKIGSALLTADGKGLDRTAMGVWVEQMVALHEAGVELVLVSSGAVAA
CCCCCCCHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHH
GMSRLGWTARPSAMHELQAAAAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHH
NARSTLRALVELKVIPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMF
HHHHHHHHHHHEEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
DADPRSNPDAQLIYEARADDPTLDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVG
CCCCCCCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEC
GRLERVLDRLKAGERIGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVSALSQGNKS
CHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCC
LLPVGVKLVQGSFRRGEMVVCVAPDGREIARGLANYSALEAQKIIGQSSDAIVGLLGYMA
CCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
EPELVHRDNLILV
CCCEEECCCEEEC
>Mature Secondary Structure
MMRSKVTGAQRWVVKIGSALLTADGKGLDRTAMGVWVEQMVALHEAGVELVLVSSGAVAA
CCCCCCCHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHH
GMSRLGWTARPSAMHELQAAAAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYL
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHH
NARSTLRALVELKVIPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMF
HHHHHHHHHHHEEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
DADPRSNPDAQLIYEARADDPTLDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVG
CCCCCCCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEC
GRLERVLDRLKAGERIGTLLSPERGMLAARKQWLAGHLQTRGTLVLDDGAVSALSQGNKS
CHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCC
LLPVGVKLVQGSFRRGEMVVCVAPDGREIARGLANYSALEAQKIIGQSSDAIVGLLGYMA
CCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
EPELVHRDNLILV
CCCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA