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The map label for this gene is panB

Identifier: 77461032

GI number: 77461032

Start: 5427974

End: 5428774

Strand: Direct

Name: panB

Synonym: Pfl01_4811

Alternate gene names: 77461032

Gene position: 5427974-5428774 (Clockwise)

Preceding gene: 77461031

Following gene: 77461033

Centisome position: 84.31

GC content: 62.8

Gene sequence:

>801_bases
ATGCCAGCCATCACCCTGACCACGCTCCAGAGCCTCAAGCAGAAAGGTGAAAAGATCACCATGCTGACCTGCTATGACGC
GACCTTCGCCCACGCCTGCAATGAGGCCGGTGTCGAAGTGCTGCTGGTGGGCGACTCCCTCGGCATGGTCCTGCAGGGTC
ACGACAGCACCCTGCCGGTGACCACCGCAGAAATGGCCTACCACGTTGCCAGCGTCAAACGCGGCAACTCTGATGCCCTG
ATCCTGGCCGACCTGCCGTTCATGGCCAACGCCACCCTCGAGCAAACCATGACCAACAGTGCCCTGCTGATGCAGGCCGG
CGCGCACATGGTCAAGGTCGAAGGCGCCTTGTGGCTGGCGGACTCGATCCGCCTGCTGGCCGAACGCGGCGTGCCGGTCT
GCGCTCACATGGGCCTGACCCCGCAAGCCGTGAACATTCTCGGTGGCTATAAAGTGCAGGGTCGCAACGAGAACCAGGCA
CGCCAGATGCGCGCCGACGCGATCTCCCTGGAGCAGGCCGGCGCGGCCATGCTGCTGCTCGAATGCGTGCCGAGCGAACT
GGCGGCTGAAATCACCCAGGCGGTGAAGATTCCGGTGATCGGCATTGGCGCCGGCAGCGACACCGACGGCCAGGTGCTGG
TACTGCACGACATGCTCGGCCTGTCGATCAGCGGCCGTGTGCCAAAATTCGTGAAGAACTTCATGCAGGGTCAGGACAGC
ATCCAGTCCGCGCTGAAGGCTTACGTCAGTGAAGTCAAAGCCACCACTTTCCCCGGGATCGAACACGGATTCTCTGCATG
A

Upstream 100 bases:

>100_bases
GGCCTTATAAGCACGACAAAAGAGCGTGCGCCTGAATAGATGACGAATCACGCGCGTTACTCGCAGTAGTTTCCAGAGCG
CCTGAACGAGGATTTCTTAC

Downstream 100 bases:

>100_bases
ACACCGTCAAAACCGTACGTGAACTGCGCGCCGCCGTGGCGCGCGCCCGCAGTGAAGGCAAGCGCATCGGCTTCGTGCCG
ACCATGGGCAACCTGCACAG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPAITLTTLQSLKQKGEKITMLTCYDATFAHACNEAGVEVLLVGDSLGMVLQGHDSTLPVTTAEMAYHVASVKRGNSDAL
ILADLPFMANATLEQTMTNSALLMQAGAHMVKVEGALWLADSIRLLAERGVPVCAHMGLTPQAVNILGGYKVQGRNENQA
RQMRADAISLEQAGAAMLLLECVPSELAAEITQAVKIPVIGIGAGSDTDGQVLVLHDMLGLSISGRVPKFVKNFMQGQDS
IQSALKAYVSEVKATTFPGIEHGFSA

Sequences:

>Translated_266_residues
MPAITLTTLQSLKQKGEKITMLTCYDATFAHACNEAGVEVLLVGDSLGMVLQGHDSTLPVTTAEMAYHVASVKRGNSDAL
ILADLPFMANATLEQTMTNSALLMQAGAHMVKVEGALWLADSIRLLAERGVPVCAHMGLTPQAVNILGGYKVQGRNENQA
RQMRADAISLEQAGAAMLLLECVPSELAAEITQAVKIPVIGIGAGSDTDGQVLVLHDMLGLSISGRVPKFVKNFMQGQDS
IQSALKAYVSEVKATTFPGIEHGFSA
>Mature_265_residues
PAITLTTLQSLKQKGEKITMLTCYDATFAHACNEAGVEVLLVGDSLGMVLQGHDSTLPVTTAEMAYHVASVKRGNSDALI
LADLPFMANATLEQTMTNSALLMQAGAHMVKVEGALWLADSIRLLAERGVPVCAHMGLTPQAVNILGGYKVQGRNENQAR
QMRADAISLEQAGAAMLLLECVPSELAAEITQAVKIPVIGIGAGSDTDGQVLVLHDMLGLSISGRVPKFVKNFMQGQDSI
QSALKAYVSEVKATTFPGIEHGFSA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=54.5454545454545, Blast_Score=313, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6319653, Length=267, Percent_Identity=39.3258426966292, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_PSEPF (Q3K6Q6)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350539.1
- ProteinModelPortal:   Q3K6Q6
- SMR:   Q3K6Q6
- STRING:   Q3K6Q6
- GeneID:   3717247
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4811
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   PFLU205922:PFL_4811-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28105; Mature: 27974

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 84-84 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAITLTTLQSLKQKGEKITMLTCYDATFAHACNEAGVEVLLVGDSLGMVLQGHDSTLPV
CCCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCE
TTAEMAYHVASVKRGNSDALILADLPFMANATLEQTMTNSALLMQAGAHMVKVEGALWLA
EHHHHHHHHHHHCCCCCCEEEEEECCCHHCCHHHHHHCCCHHHHHCCCEEEEECCEEEHH
DSIRLLAERGVPVCAHMGLTPQAVNILGGYKVQGRNENQARQMRADAISLEQAGAAMLLL
HHHHHHHHCCCCEEEECCCCHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
ECVPSELAAEITQAVKIPVIGIGAGSDTDGQVLVLHDMLGLSISGRVPKFVKNFMQGQDS
HHCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCHHH
IQSALKAYVSEVKATTFPGIEHGFSA
HHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PAITLTTLQSLKQKGEKITMLTCYDATFAHACNEAGVEVLLVGDSLGMVLQGHDSTLPV
CCCHHHHHHHHHHCCCEEEEEEEECCHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCE
TTAEMAYHVASVKRGNSDALILADLPFMANATLEQTMTNSALLMQAGAHMVKVEGALWLA
EHHHHHHHHHHHCCCCCCEEEEEECCCHHCCHHHHHHCCCHHHHHCCCEEEEECCEEEHH
DSIRLLAERGVPVCAHMGLTPQAVNILGGYKVQGRNENQARQMRADAISLEQAGAAMLLL
HHHHHHHHCCCCEEEECCCCHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHH
ECVPSELAAEITQAVKIPVIGIGAGSDTDGQVLVLHDMLGLSISGRVPKFVKNFMQGQDS
HHCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCHHH
IQSALKAYVSEVKATTFPGIEHGFSA
HHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA