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The map label for this gene is mrcB [H]

Identifier: 77461013

GI number: 77461013

Start: 5406750

End: 5409074

Strand: Reverse

Name: mrcB [H]

Synonym: Pfl01_4792

Alternate gene names: 77461013

Gene position: 5409074-5406750 (Counterclockwise)

Preceding gene: 77461018

Following gene: 77461012

Centisome position: 84.01

GC content: 61.51

Gene sequence:

>2325_bases
ATGACTCGTACTCGATCCCCCCGTACCCCTAAAAAACCAGCTTCCCGGGGCCTGCGCCCATGGTTGGGCTGGGCCCTTAA
ACTCAGTCTGGTCGGCCTTGTGGTGCTGGCCGGCTTCGCCGTTTACCTCGATGCGGTGGTGCAGGAGAAGTTCTCCGGCA
AGCGCTGGACCATCCCGGCCAAGGTATACGCGCGTCCGCTCGAGCTGTTCGTCGGACAAAAGCTGAGCAAGGACGATTTC
CTCACCGAACTCGATGCCTTGGGCTATCGCCGCGAAGCTGTGAGCAACGGTCCCGGCGCGGCGGCGGTCAATGGCAACAC
GGTTGATCTGAACACCCGTGGCTTCCAGTTCTATGAAGGCCTCGAGAAACCGCAGCCGGTGCGCGTGCGTTTCTCCGGCG
ATTACGTGGCCGAACTCTCGGCGACCAACGGTTCGAAGCTTTCCGTGGTGCGCCTGGAGCCGCTGATGATCGGCGGTATC
TACCCGAAAAACCTTGAAGACCGGATTCTGATCAAGCTCGATCAGGTGCCGCCGTATCTGCTGGAAACCCTGGTCGCCGT
GGAAGACCGGGATTTCTACAGCCACTGGGGCGTATCGCCGAAATCGATTGCCCGCGCCGTCTGGGTCAACACTTCGGGCG
GCAAGATGACTCAGGGCGGCAGTACGCTGACCCAGCAACTGGTCAAGAACTTCTACCTCACCAACGAACGCAGCCTGACC
CGCAAGCTCACCGAAGCGATGATGGCGATGCTGCTCGAGCTGCATTACGACAAAAAGGAAATCCTCGAGGCTTACCTCAA
CGAAGTCTTTGTCGGTCAGGACGGTCAGCGTGCGGTGCACGGTTTCGGTCTGGCCAGCCAGTTCTTCTTTGCTCAGCCTT
TGTCCGAGCTGAAGCTGCACCAGGTCGCGCTGTTGGTCGGGATGGTCAAAGGCCCGTCCTATTACAACCCGCGCCGCAAT
CCGGAGCGGGCGCTGGAGCGACGCAATCTGGTGCTCGACGTGCTTGAACAGCAGGGCGTCGCTTCCGCCGAACAAGTGGC
TGCCGCGAAGAAAATGCCGCTGGGCGTAACCACGCGCGGCAAGCTGGCCGACAGTTCGTTTCCGGGTTTCATCGATCTGG
TCAAGCGCCAGTTGCGTGAAGACTACCGCGACGAAGACTTGACCGAAGAAGGCCTGCGGATTTTCACCAGCTTCGATCCG
ATTCTGCAGATGAAAGCCGAAGCGTCGGTCAACGACACTTTCAAGCGCCTGTCCGGTCGCAAGGGTTCCGACGACGTGGA
AGCGGCGATGGTCGTTACCAACCCGGAAACCGGCGAAGTCCAGGCCATGATCGGCAGCCGTCAGGCGAGTTTTGCCGGGT
TCAACCGGGCACTGGATGCGGTGCGGCCGATTGGCTCGCTGATCAAACCGGCGGTTTATCTGACGGCGCTGGAGAAGCCG
AGCCAGTACACGTTGACCAGTTGGCTGTCGGACGAGCCGCTTTCAATCAAGGGTGCGGATGGTCAGGTCTGGAAACCGCA
GAACTATGACCGCCGCTCCCACGGTACGGTGTTCCTGTATCAGGGGCTGGCACATTCCTACAACCTGTCGACCTCGCGCC
TCGGTCTGGCGGTTGGCGTTCCGAATGTCCTCAAGACGCTGGGTCGCCTGGGCGTGACCCGCGAGTTTCCGGCGTTCCCG
TCGATGCTGCTCGGTGCGGGCGGAATGACCCCGATTGAAGTCGCGACCATGTACCAAACGCTTGCCAACGGTGGTTTCAA
TACGCCGATGCGCGGGATTCGCAGCGTGCTGACTGCCGACGGCGAACCGCTCAAGCGTTATCCGTTCCAGATCGAGCAGC
GTTTCGATCCGGCCTCGATCTATCTGATCCAGAACGCCATGCAGCGGGTCATGCGTGAAGGCACCGGCAGTTCGGTTTAT
AACGTGTTGCCAAGAACCCTGACGCTGGCGGGCAAGACCGGCACCAGTAACGATTCGCGCGACAGCTGGTTCGCCGGCTT
CAGCCAGGATCTGCTGGCGGTGGTCTGGCTCGGCCGCGACGACAACGGCAAGACGCCGTTCACCGGGGCCACCGGTGCGT
TGCAGGTCTGGACCAGCTTCATGCGCAAGGCCGATCCGCTGCCGCTGGACATGCCGCAACCGGATAACGTGGTGCAGGCC
TGGGTCGATTCGCGTACCGGCCAGGGATCTGATGCCAACTGTCCGGGCGCCGTGCAGATGCCGTATATTCGCGGCAGCGA
ACCGCCACCCGGCGCCTCCTGCGCGGGCGAAAGCCCGGCCTCGGGCGAGTCGGTGATGGATTGGGTCAAGGGCTGGATGA
ATTAA

Upstream 100 bases:

>100_bases
TGGGGGAAGTTCAAGGCGCTCATTATGGCCGCTCGCCAGCCCGACGCAAACCTCGGAGCGCTCCTGTTGAGTATGAATAA
AGTGCGTATAATCCGCCGCC

Downstream 100 bases:

>100_bases
GCAAAGAGGGTTTCAAGTGAACAAGTGGTTGATTCCAGCGGTGACCGCCGTGGCTTTGCTCAGCGGCTGCTCCACCGTAC
AGCGTGGTTCGATCCCGGTT

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 774; Mature: 773

Protein sequence:

>774_residues
MTRTRSPRTPKKPASRGLRPWLGWALKLSLVGLVVLAGFAVYLDAVVQEKFSGKRWTIPAKVYARPLELFVGQKLSKDDF
LTELDALGYRREAVSNGPGAAAVNGNTVDLNTRGFQFYEGLEKPQPVRVRFSGDYVAELSATNGSKLSVVRLEPLMIGGI
YPKNLEDRILIKLDQVPPYLLETLVAVEDRDFYSHWGVSPKSIARAVWVNTSGGKMTQGGSTLTQQLVKNFYLTNERSLT
RKLTEAMMAMLLELHYDKKEILEAYLNEVFVGQDGQRAVHGFGLASQFFFAQPLSELKLHQVALLVGMVKGPSYYNPRRN
PERALERRNLVLDVLEQQGVASAEQVAAAKKMPLGVTTRGKLADSSFPGFIDLVKRQLREDYRDEDLTEEGLRIFTSFDP
ILQMKAEASVNDTFKRLSGRKGSDDVEAAMVVTNPETGEVQAMIGSRQASFAGFNRALDAVRPIGSLIKPAVYLTALEKP
SQYTLTSWLSDEPLSIKGADGQVWKPQNYDRRSHGTVFLYQGLAHSYNLSTSRLGLAVGVPNVLKTLGRLGVTREFPAFP
SMLLGAGGMTPIEVATMYQTLANGGFNTPMRGIRSVLTADGEPLKRYPFQIEQRFDPASIYLIQNAMQRVMREGTGSSVY
NVLPRTLTLAGKTGTSNDSRDSWFAGFSQDLLAVVWLGRDDNGKTPFTGATGALQVWTSFMRKADPLPLDMPQPDNVVQA
WVDSRTGQGSDANCPGAVQMPYIRGSEPPPGASCAGESPASGESVMDWVKGWMN

Sequences:

>Translated_774_residues
MTRTRSPRTPKKPASRGLRPWLGWALKLSLVGLVVLAGFAVYLDAVVQEKFSGKRWTIPAKVYARPLELFVGQKLSKDDF
LTELDALGYRREAVSNGPGAAAVNGNTVDLNTRGFQFYEGLEKPQPVRVRFSGDYVAELSATNGSKLSVVRLEPLMIGGI
YPKNLEDRILIKLDQVPPYLLETLVAVEDRDFYSHWGVSPKSIARAVWVNTSGGKMTQGGSTLTQQLVKNFYLTNERSLT
RKLTEAMMAMLLELHYDKKEILEAYLNEVFVGQDGQRAVHGFGLASQFFFAQPLSELKLHQVALLVGMVKGPSYYNPRRN
PERALERRNLVLDVLEQQGVASAEQVAAAKKMPLGVTTRGKLADSSFPGFIDLVKRQLREDYRDEDLTEEGLRIFTSFDP
ILQMKAEASVNDTFKRLSGRKGSDDVEAAMVVTNPETGEVQAMIGSRQASFAGFNRALDAVRPIGSLIKPAVYLTALEKP
SQYTLTSWLSDEPLSIKGADGQVWKPQNYDRRSHGTVFLYQGLAHSYNLSTSRLGLAVGVPNVLKTLGRLGVTREFPAFP
SMLLGAGGMTPIEVATMYQTLANGGFNTPMRGIRSVLTADGEPLKRYPFQIEQRFDPASIYLIQNAMQRVMREGTGSSVY
NVLPRTLTLAGKTGTSNDSRDSWFAGFSQDLLAVVWLGRDDNGKTPFTGATGALQVWTSFMRKADPLPLDMPQPDNVVQA
WVDSRTGQGSDANCPGAVQMPYIRGSEPPPGASCAGESPASGESVMDWVKGWMN
>Mature_773_residues
TRTRSPRTPKKPASRGLRPWLGWALKLSLVGLVVLAGFAVYLDAVVQEKFSGKRWTIPAKVYARPLELFVGQKLSKDDFL
TELDALGYRREAVSNGPGAAAVNGNTVDLNTRGFQFYEGLEKPQPVRVRFSGDYVAELSATNGSKLSVVRLEPLMIGGIY
PKNLEDRILIKLDQVPPYLLETLVAVEDRDFYSHWGVSPKSIARAVWVNTSGGKMTQGGSTLTQQLVKNFYLTNERSLTR
KLTEAMMAMLLELHYDKKEILEAYLNEVFVGQDGQRAVHGFGLASQFFFAQPLSELKLHQVALLVGMVKGPSYYNPRRNP
ERALERRNLVLDVLEQQGVASAEQVAAAKKMPLGVTTRGKLADSSFPGFIDLVKRQLREDYRDEDLTEEGLRIFTSFDPI
LQMKAEASVNDTFKRLSGRKGSDDVEAAMVVTNPETGEVQAMIGSRQASFAGFNRALDAVRPIGSLIKPAVYLTALEKPS
QYTLTSWLSDEPLSIKGADGQVWKPQNYDRRSHGTVFLYQGLAHSYNLSTSRLGLAVGVPNVLKTLGRLGVTREFPAFPS
MLLGAGGMTPIEVATMYQTLANGGFNTPMRGIRSVLTADGEPLKRYPFQIEQRFDPASIYLIQNAMQRVMREGTGSSVYN
VLPRTLTLAGKTGTSNDSRDSWFAGFSQDLLAVVWLGRDDNGKTPFTGATGALQVWTSFMRKADPLPLDMPQPDNVVQAW
VDSRTGQGSDANCPGAVQMPYIRGSEPPPGASCAGESPASGESVMDWVKGWMN

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=734, Percent_Identity=41.008174386921, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1788867, Length=597, Percent_Identity=29.3132328308208, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87082258, Length=255, Percent_Identity=34.9019607843137, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789601, Length=169, Percent_Identity=32.5443786982249, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 85148; Mature: 85017

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTRSPRTPKKPASRGLRPWLGWALKLSLVGLVVLAGFAVYLDAVVQEKFSGKRWTIPA
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCH
KVYARPLELFVGQKLSKDDFLTELDALGYRREAVSNGPGAAAVNGNTVDLNTRGFQFYEG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCHHHHHC
LEKPQPVRVRFSGDYVAELSATNGSKLSVVRLEPLMIGGIYPKNLEDRILIKLDQVPPYL
CCCCCCEEEEECCCEEEEEECCCCCEEEEEEECCEEEECCCCCCCCCEEEEEECCCCHHH
LETLVAVEDRDFYSHWGVSPKSIARAVWVNTSGGKMTQGGSTLTQQLVKNFYLTNERSLT
HHHHHHHCCCCHHHHCCCCHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
RKLTEAMMAMLLELHYDKKEILEAYLNEVFVGQDGQRAVHGFGLASQFFFAQPLSELKLH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHH
QVALLVGMVKGPSYYNPRRNPERALERRNLVLDVLEQQGVASAEQVAAAKKMPLGVTTRG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
KLADSSFPGFIDLVKRQLREDYRDEDLTEEGLRIFTSFDPILQMKAEASVNDTFKRLSGR
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHEEHHCCCHHHHHHHCCCHHHHHHHHCCC
KGSDDVEAAMVVTNPETGEVQAMIGSRQASFAGFNRALDAVRPIGSLIKPAVYLTALEKP
CCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SQYTLTSWLSDEPLSIKGADGQVWKPQNYDRRSHGTVFLYQGLAHSYNLSTSRLGLAVGV
CCEEHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCEEEECC
PNVLKTLGRLGVTREFPAFPSMLLGAGGMTPIEVATMYQTLANGGFNTPMRGIRSVLTAD
HHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
GEPLKRYPFQIEQRFDPASIYLIQNAMQRVMREGTGSSVYNVLPRTLTLAGKTGTSNDSR
CCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCHHEEEECCCCCCCCCC
DSWFAGFSQDLLAVVWLGRDDNGKTPFTGATGALQVWTSFMRKADPLPLDMPQPDNVVQA
CHHHHCCHHCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
WVDSRTGQGSDANCPGAVQMPYIRGSEPPPGASCAGESPASGESVMDWVKGWMN
HHHCCCCCCCCCCCCCCEECCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TRTRSPRTPKKPASRGLRPWLGWALKLSLVGLVVLAGFAVYLDAVVQEKFSGKRWTIPA
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCH
KVYARPLELFVGQKLSKDDFLTELDALGYRREAVSNGPGAAAVNGNTVDLNTRGFQFYEG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCHHHHHC
LEKPQPVRVRFSGDYVAELSATNGSKLSVVRLEPLMIGGIYPKNLEDRILIKLDQVPPYL
CCCCCCEEEEECCCEEEEEECCCCCEEEEEEECCEEEECCCCCCCCCEEEEEECCCCHHH
LETLVAVEDRDFYSHWGVSPKSIARAVWVNTSGGKMTQGGSTLTQQLVKNFYLTNERSLT
HHHHHHHCCCCHHHHCCCCHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
RKLTEAMMAMLLELHYDKKEILEAYLNEVFVGQDGQRAVHGFGLASQFFFAQPLSELKLH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHH
QVALLVGMVKGPSYYNPRRNPERALERRNLVLDVLEQQGVASAEQVAAAKKMPLGVTTRG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
KLADSSFPGFIDLVKRQLREDYRDEDLTEEGLRIFTSFDPILQMKAEASVNDTFKRLSGR
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHEEHHCCCHHHHHHHCCCHHHHHHHHCCC
KGSDDVEAAMVVTNPETGEVQAMIGSRQASFAGFNRALDAVRPIGSLIKPAVYLTALEKP
CCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SQYTLTSWLSDEPLSIKGADGQVWKPQNYDRRSHGTVFLYQGLAHSYNLSTSRLGLAVGV
CCEEHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHCEEEECC
PNVLKTLGRLGVTREFPAFPSMLLGAGGMTPIEVATMYQTLANGGFNTPMRGIRSVLTAD
HHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
GEPLKRYPFQIEQRFDPASIYLIQNAMQRVMREGTGSSVYNVLPRTLTLAGKTGTSNDSR
CCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCHHEEEECCCCCCCCCC
DSWFAGFSQDLLAVVWLGRDDNGKTPFTGATGALQVWTSFMRKADPLPLDMPQPDNVVQA
CHHHHCCHHCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
WVDSRTGQGSDANCPGAVQMPYIRGSEPPPGASCAGESPASGESVMDWVKGWMN
HHHCCCCCCCCCCCCCCEECCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]