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The map label for this gene is ilvI [H]

Identifier: 77461009

GI number: 77461009

Start: 5403092

End: 5404816

Strand: Reverse

Name: ilvI [H]

Synonym: Pfl01_4788

Alternate gene names: 77461009

Gene position: 5404816-5403092 (Counterclockwise)

Preceding gene: 77461011

Following gene: 77461008

Centisome position: 83.95

GC content: 57.68

Gene sequence:

>1725_bases
GTGGAGCTTTTATCTGGCGGTGAGATGCTCGTCCGCTTTTTGCGTGACGAAGGCGTCAAATATATCTACGGGTACCCGGG
TGGTGCTCTCCTTCATGTTTACGATGCCCTGTTCAAAGAGCCGGAAGTGACTCACATCCTGGTTCGCCACGAACAAGCGG
CTACCCATATGGCTGACGGCTACGCCCGTGCCACCGGTAAAGCCGGCGTGGTATTGGTGACTTCCGGTCCAGGCGCCACA
AACGCCATCACCGGTATCGCCACCGCCTATATGGACTCGATTCCAATGGTGATCATTTCCGGTCAGGTGCCAAGCACCAT
GGTCGGCACCGATGCGTTCCAGGAAACCGACATGATCGGTATCTCACGGCCGATCGTGAAGCACAGCTTCATGATCAAGC
ACGCTTCGGAAATCCCGGAAGTCATGAAGAAAGCCTTCTACCTGGCGCAATCCGGTCGTCCGGGCCCGGTCGTGGTCGAT
ATCCCGAAAGACATGACCAACCCGGCCGAGAAGTTCGAATACATCTTCCCGAAGAAAGCCAAACTGCGTTCCTACAGCCC
GGCCGTTCGCGGTCACTCCGGGCAAATCCGCAAAGCGGCAGAAATGCTCCTGGCGGCCAAGCGTCCCGTGCTGTACTCCG
GCGGTGGCGTGATCCTCGGCAACGGCTCCGCGCCGCTGACCGAACTGGCAAAAATGCTCAACCTGCCGGTTACCAATACC
TTGATGGGCCTGGGTGCCTATCCTGGCACTGATCGTCAGTTCATCGGCATGCTCGGCATGCACGGCAGCTATACCGCCAA
CCTGGCGATGCACCATGCCGACGTGATCCTGGCGGTCGGTGCGCGTTTTGACGATCGCGTGATCAACGGCGCGCCGAAGT
TTTGCCCGAACGCCAAGATCATCCATATCGACATCGACCCGGCTTCGATTTCCAAGACCATCAAGGCCGACGTGCCAATC
GTTGGGCCGGTGGAGAGCGTCCTGACCGAAATGGTCGCGATCCTCAAGGAAATCGGCGAAACCCCGAACAAGGAAGCCGT
TGCCAGCTGGTGGAAGCAGGTTGATGAATGGCGCGGTGATCGTGGCCTGTTCCCTTACGACAAGGGCGACGGCAGCAAGA
TCAAGCCACAGACCGTGATCGAGACCCTGTGCGAAGTGACCAAGGGCGATGCCTTTGTGACCTCGGACGTGGGCCAGCAC
CAGATGTTTGCTGCGCAGTACTACAAGTTCAACAAGCCTAACCGCTGGATCAACTCCGGTGGCCTGGGCACGATGGGCTT
TGGTCTGCCGGCCGCGATGGGTATCGCTTTGAGCTTCCCTGACACCGATGTTGCGTGCGTCACCGGTGAAGGCAGTATCC
AGATGAACATCCAGGAACTGTCGACCTGCCTGCAATATGGTTTGCCGGTCAAGATCGTCATCCTGAACAACGGTGTTCTG
GGCATGGTTCGTCAGTGGCAGGACATGAGTTACGGCAGCCGTCACTCGCACTCCTACATGGAATCATTGCCTGACTTCGT
CAAGCTGGCTGAAGCCTATGGCCACGTCGGCGTGCGCATCACCGAATCGAAAGATTTGAAGTCGAAGATGGAAGAGGCGT
TCGCCATGAAAGATCGCCTGGTGGTGATCGACATTTCGGTCGACACCAGCGAGCACGTCTATCCGATGCAGATCAAAGAC
GGCTCCATGCGCGATATGTGGCTGAGCAAGACGGAGCGTACCTAA

Upstream 100 bases:

>100_bases
GTCGGCGACCACCGTCGCTCATGCTCTGCCGAGAAGTAAACCTATTAAGACCCGTTCGCTTTTTTGTGGACGGTATTCTG
GCGTTTTAGAGGTGAACAAC

Downstream 100 bases:

>100_bases
TCATGCGGCACATTATTTCCTTGCTTCTGGAAAACGAACCCGGTGCTTTGTCTCGCGTAGTCGGCCTGTTCTCGCAGCGC
AACTACAACATCGAAAGCCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MELLSGGEMLVRFLRDEGVKYIYGYPGGALLHVYDALFKEPEVTHILVRHEQAATHMADGYARATGKAGVVLVTSGPGAT
NAITGIATAYMDSIPMVIISGQVPSTMVGTDAFQETDMIGISRPIVKHSFMIKHASEIPEVMKKAFYLAQSGRPGPVVVD
IPKDMTNPAEKFEYIFPKKAKLRSYSPAVRGHSGQIRKAAEMLLAAKRPVLYSGGGVILGNGSAPLTELAKMLNLPVTNT
LMGLGAYPGTDRQFIGMLGMHGSYTANLAMHHADVILAVGARFDDRVINGAPKFCPNAKIIHIDIDPASISKTIKADVPI
VGPVESVLTEMVAILKEIGETPNKEAVASWWKQVDEWRGDRGLFPYDKGDGSKIKPQTVIETLCEVTKGDAFVTSDVGQH
QMFAAQYYKFNKPNRWINSGGLGTMGFGLPAAMGIALSFPDTDVACVTGEGSIQMNIQELSTCLQYGLPVKIVILNNGVL
GMVRQWQDMSYGSRHSHSYMESLPDFVKLAEAYGHVGVRITESKDLKSKMEEAFAMKDRLVVIDISVDTSEHVYPMQIKD
GSMRDMWLSKTERT

Sequences:

>Translated_574_residues
MELLSGGEMLVRFLRDEGVKYIYGYPGGALLHVYDALFKEPEVTHILVRHEQAATHMADGYARATGKAGVVLVTSGPGAT
NAITGIATAYMDSIPMVIISGQVPSTMVGTDAFQETDMIGISRPIVKHSFMIKHASEIPEVMKKAFYLAQSGRPGPVVVD
IPKDMTNPAEKFEYIFPKKAKLRSYSPAVRGHSGQIRKAAEMLLAAKRPVLYSGGGVILGNGSAPLTELAKMLNLPVTNT
LMGLGAYPGTDRQFIGMLGMHGSYTANLAMHHADVILAVGARFDDRVINGAPKFCPNAKIIHIDIDPASISKTIKADVPI
VGPVESVLTEMVAILKEIGETPNKEAVASWWKQVDEWRGDRGLFPYDKGDGSKIKPQTVIETLCEVTKGDAFVTSDVGQH
QMFAAQYYKFNKPNRWINSGGLGTMGFGLPAAMGIALSFPDTDVACVTGEGSIQMNIQELSTCLQYGLPVKIVILNNGVL
GMVRQWQDMSYGSRHSHSYMESLPDFVKLAEAYGHVGVRITESKDLKSKMEEAFAMKDRLVVIDISVDTSEHVYPMQIKD
GSMRDMWLSKTERT
>Mature_574_residues
MELLSGGEMLVRFLRDEGVKYIYGYPGGALLHVYDALFKEPEVTHILVRHEQAATHMADGYARATGKAGVVLVTSGPGAT
NAITGIATAYMDSIPMVIISGQVPSTMVGTDAFQETDMIGISRPIVKHSFMIKHASEIPEVMKKAFYLAQSGRPGPVVVD
IPKDMTNPAEKFEYIFPKKAKLRSYSPAVRGHSGQIRKAAEMLLAAKRPVLYSGGGVILGNGSAPLTELAKMLNLPVTNT
LMGLGAYPGTDRQFIGMLGMHGSYTANLAMHHADVILAVGARFDDRVINGAPKFCPNAKIIHIDIDPASISKTIKADVPI
VGPVESVLTEMVAILKEIGETPNKEAVASWWKQVDEWRGDRGLFPYDKGDGSKIKPQTVIETLCEVTKGDAFVTSDVGQH
QMFAAQYYKFNKPNRWINSGGLGTMGFGLPAAMGIALSFPDTDVACVTGEGSIQMNIQELSTCLQYGLPVKIVILNNGVL
GMVRQWQDMSYGSRHSHSYMESLPDFVKLAEAYGHVGVRITESKDLKSKMEEAFAMKDRLVVIDISVDTSEHVYPMQIKD
GSMRDMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=25.9325044404973, Blast_Score=172, Evalue=9e-43,
Organism=Homo sapiens, GI21361361, Length=563, Percent_Identity=25.044404973357, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI87081685, Length=576, Percent_Identity=63.0208333333333, Blast_Score=779, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=42.4187725631769, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=34.0823970037453, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=28.2882882882883, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=24.0143369175627, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=590, Percent_Identity=27.1186440677966, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=590, Percent_Identity=27.1186440677966, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=574, Percent_Identity=26.3066202090592, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=590, Percent_Identity=43.3898305084746, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320816, Length=487, Percent_Identity=23.4086242299795, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321524, Length=501, Percent_Identity=22.3552894211577, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922626, Length=586, Percent_Identity=26.7918088737201, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62567; Mature: 62567

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLSGGEMLVRFLRDEGVKYIYGYPGGALLHVYDALFKEPEVTHILVRHEQAATHMADG
CCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCC
YARATGKAGVVLVTSGPGATNAITGIATAYMDSIPMVIISGQVPSTMVGTDAFQETDMIG
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHCCCHHHHCCCCC
ISRPIVKHSFMIKHASEIPEVMKKAFYLAQSGRPGPVVVDIPKDMTNPAEKFEYIFPKKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHCCCCCCH
KLRSYSPAVRGHSGQIRKAAEMLLAAKRPVLYSGGGVILGNGSAPLTELAKMLNLPVTNT
HHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCEEEEECCCCCHHHHHHHHCCCHHHH
LMGLGAYPGTDRQFIGMLGMHGSYTANLAMHHADVILAVGARFDDRVINGAPKFCPNAKI
HHHCCCCCCCCHHHHHHHCCCCCEEEEEHHHHCEEEEEECCCCCCHHHCCCCCCCCCCEE
IHIDIDPASISKTIKADVPIVGPVESVLTEMVAILKEIGETPNKEAVASWWKQVDEWRGD
EEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
RGLFPYDKGDGSKIKPQTVIETLCEVTKGDAFVTSDVGQHQMFAAQYYKFNKPNRWINSG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCEECCC
GLGTMGFGLPAAMGIALSFPDTDVACVTGEGSIQMNIQELSTCLQYGLPVKIVILNNGVL
CCCCCCCCCHHHHCEEEECCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCHH
GMVRQWQDMSYGSRHSHSYMESLPDFVKLAEAYGHVGVRITESKDLKSKMEEAFAMKDRL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCE
VVIDISVDTSEHVYPMQIKDGSMRDMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCHHHHHHCCCCC
>Mature Secondary Structure
MELLSGGEMLVRFLRDEGVKYIYGYPGGALLHVYDALFKEPEVTHILVRHEQAATHMADG
CCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCC
YARATGKAGVVLVTSGPGATNAITGIATAYMDSIPMVIISGQVPSTMVGTDAFQETDMIG
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHCCCHHHHCCCCC
ISRPIVKHSFMIKHASEIPEVMKKAFYLAQSGRPGPVVVDIPKDMTNPAEKFEYIFPKKA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHCCCCCCH
KLRSYSPAVRGHSGQIRKAAEMLLAAKRPVLYSGGGVILGNGSAPLTELAKMLNLPVTNT
HHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCEEEEECCCCCHHHHHHHHCCCHHHH
LMGLGAYPGTDRQFIGMLGMHGSYTANLAMHHADVILAVGARFDDRVINGAPKFCPNAKI
HHHCCCCCCCCHHHHHHHCCCCCEEEEEHHHHCEEEEEECCCCCCHHHCCCCCCCCCCEE
IHIDIDPASISKTIKADVPIVGPVESVLTEMVAILKEIGETPNKEAVASWWKQVDEWRGD
EEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
RGLFPYDKGDGSKIKPQTVIETLCEVTKGDAFVTSDVGQHQMFAAQYYKFNKPNRWINSG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCEECCC
GLGTMGFGLPAAMGIALSFPDTDVACVTGEGSIQMNIQELSTCLQYGLPVKIVILNNGVL
CCCCCCCCCHHHHCEEEECCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCHH
GMVRQWQDMSYGSRHSHSYMESLPDFVKLAEAYGHVGVRITESKDLKSKMEEAFAMKDRL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCE
VVIDISVDTSEHVYPMQIKDGSMRDMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]