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The map label for this gene is algI [H]

Identifier: 77460860

GI number: 77460860

Start: 5237584

End: 5239065

Strand: Direct

Name: algI [H]

Synonym: Pfl01_4639

Alternate gene names: 77460860

Gene position: 5237584-5239065 (Clockwise)

Preceding gene: 77460859

Following gene: 77460861

Centisome position: 81.35

GC content: 53.24

Gene sequence:

>1482_bases
ATGACCTTCAGCTCACCCAAGTTCTTCCTGATCTACCTGCCCATTGTCTTTTTTGTGTACTTCTTCCTGAATCGTCAGCG
GCTGGTCACAGCTGGAAAAGTCTGGCTTGTCATTGCCAGTCTGATTTTCTATGGGCGCTGGAGCGTCGCTTACATTCCCT
TGCTCATGTTCTCCATCGGCTTCAACTTCATAGTCGGAAAAGCCCTCGCCGCCCCCGACAACACCCTGCCGTCAGGCCTG
TCGAAAAGGGCCATTCTGGGCATTGGAATAGTCACTGACCTGGCCTTGCTCGGGTATTTCAAATACACCAATTTCCTGGT
CAACAACATCAACTCGGTGCTCGGAACCGAGTTGTTTTTCTACCAGGTCATTTTGCCTCTGGGCATCAGCTTCTACACGT
TTACGCAAATCGCCTTTCTGGTGGACTGCTACAAGAACCTCGCGAAAGAGTACAGCTTCGTCAACTATGCGCTGTTCGTA
ACGTTCTTCCCTCACCTGATCGCCGGGCCGATTCTTCACCATAAAGAAATGATGAGTCAGTTCCAGTCACGCTGGACGCT
CGTCATTCGCCACAGAAACGTGGTCATGGGCCTGTTCATTTTCAGCATCGGTCTGTTCAAGAAAGTGATGATTGCCGACA
CCTTTGCCATTTGGGCGGATGCCGGATTCGCTCAGGGTGTCAGTCATGATTTTTTCAGCGCGTGGACGACCAGTCTTTCC
TACACCTTTCAGCTCTACTTCGACTTCAGCGGTTACTGCGACATGGCAATAGGCGCCGCGTTGCTGTTCAACATATGGCT
GCCGCTGAACTTCAACTCACCTTACAAGGCGCTGGACATCCAGGACTTCTGGCGGCGCTGGCACATGACGTTGAGCCGTT
ATCTTCGCGACTACCTATACGTACCGCTGGGCGGTAACCGGTGCGGCCGGCAGCGCTTGTATTTCAATCTGATGGTGACT
TTCGTTCTCGGCGGGCTCTGGCATGGTGCGAGCTGGATGTTCATCATCTGGGGGGCATTGCATGGCGGCGCCATTGTCAT
GCACCGGATCTGGAAACAAGTGGGCCTGTCGCTGCCCGACCCTGTCGGATGGTTCGTCACGTTCATGTTCGTGAACATCA
CATGGGTGTTTTTCAGAGCCGATTCCGTTGACGACGCACTGCGCATATTGCACGGGATGATTGATTTCGACTCTATCGAT
CAGCTCTCCGTAAACACCGTCCCGACTGCCAACCTTGCCTGGGGAGGGACGCTATCCGATCGCTTGCTCGAGACCCTTCC
CACAGGCCTGACAGCCAATCTTGCTCCGACATTGATGATTGCCGTCGCGCTGTTGATCATCCGCTGTAAAAACGCTTTCG
AACTCACCGCTCACACTGATTTTGGCAAGGCGAAGACGCTCGCCATGTCCACGCTGTTTTGTCTTTCGCTGTACGCCAGC
GTGCAAAGCAAAAGCCCCGTTTTCCTCTACTTCAACTTCTAG

Upstream 100 bases:

>100_bases
GTAGGAACCGTCTCGACCTGCATTTTCGGCATTCGCACACTCATCGAGCCTGAATACAATCCGCCGACTTCTGAAAGCGC
GGACGCATTCAAGGCCCCTC

Downstream 100 bases:

>100_bases
GGGCTCGCCTGTGAAAAGACAGTTATATGCGGCACTCACCCTGACTGCATTGATCATGTGCACTGTTCCGACCGTCAATC
TGTACCAGGTGTTTACGACA

Product: membrane bound O-acyl transferase, MBOAT

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTFSSPKFFLIYLPIVFFVYFFLNRQRLVTAGKVWLVIASLIFYGRWSVAYIPLLMFSIGFNFIVGKALAAPDNTLPSGL
SKRAILGIGIVTDLALLGYFKYTNFLVNNINSVLGTELFFYQVILPLGISFYTFTQIAFLVDCYKNLAKEYSFVNYALFV
TFFPHLIAGPILHHKEMMSQFQSRWTLVIRHRNVVMGLFIFSIGLFKKVMIADTFAIWADAGFAQGVSHDFFSAWTTSLS
YTFQLYFDFSGYCDMAIGAALLFNIWLPLNFNSPYKALDIQDFWRRWHMTLSRYLRDYLYVPLGGNRCGRQRLYFNLMVT
FVLGGLWHGASWMFIIWGALHGGAIVMHRIWKQVGLSLPDPVGWFVTFMFVNITWVFFRADSVDDALRILHGMIDFDSID
QLSVNTVPTANLAWGGTLSDRLLETLPTGLTANLAPTLMIAVALLIIRCKNAFELTAHTDFGKAKTLAMSTLFCLSLYAS
VQSKSPVFLYFNF

Sequences:

>Translated_493_residues
MTFSSPKFFLIYLPIVFFVYFFLNRQRLVTAGKVWLVIASLIFYGRWSVAYIPLLMFSIGFNFIVGKALAAPDNTLPSGL
SKRAILGIGIVTDLALLGYFKYTNFLVNNINSVLGTELFFYQVILPLGISFYTFTQIAFLVDCYKNLAKEYSFVNYALFV
TFFPHLIAGPILHHKEMMSQFQSRWTLVIRHRNVVMGLFIFSIGLFKKVMIADTFAIWADAGFAQGVSHDFFSAWTTSLS
YTFQLYFDFSGYCDMAIGAALLFNIWLPLNFNSPYKALDIQDFWRRWHMTLSRYLRDYLYVPLGGNRCGRQRLYFNLMVT
FVLGGLWHGASWMFIIWGALHGGAIVMHRIWKQVGLSLPDPVGWFVTFMFVNITWVFFRADSVDDALRILHGMIDFDSID
QLSVNTVPTANLAWGGTLSDRLLETLPTGLTANLAPTLMIAVALLIIRCKNAFELTAHTDFGKAKTLAMSTLFCLSLYAS
VQSKSPVFLYFNF
>Mature_492_residues
TFSSPKFFLIYLPIVFFVYFFLNRQRLVTAGKVWLVIASLIFYGRWSVAYIPLLMFSIGFNFIVGKALAAPDNTLPSGLS
KRAILGIGIVTDLALLGYFKYTNFLVNNINSVLGTELFFYQVILPLGISFYTFTQIAFLVDCYKNLAKEYSFVNYALFVT
FFPHLIAGPILHHKEMMSQFQSRWTLVIRHRNVVMGLFIFSIGLFKKVMIADTFAIWADAGFAQGVSHDFFSAWTTSLSY
TFQLYFDFSGYCDMAIGAALLFNIWLPLNFNSPYKALDIQDFWRRWHMTLSRYLRDYLYVPLGGNRCGRQRLYFNLMVTF
VLGGLWHGASWMFIIWGALHGGAIVMHRIWKQVGLSLPDPVGWFVTFMFVNITWVFFRADSVDDALRILHGMIDFDSIDQ
LSVNTVPTANLAWGGTLSDRLLETLPTGLTANLAPTLMIAVALLIIRCKNAFELTAHTDFGKAKTLAMSTLFCLSLYASV
QSKSPVFLYFNF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 56189; Mature: 56058

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCEEEEEEC
>Mature Secondary Structure 
TFSSPKFFLIYLPIVFFVYFFLNRQRLVTAGKVWLVIASLIFYGRWSVAYIPLLMFSIG
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
FNFIVGKALAAPDNTLPSGLSKRAILGIGIVTDLALLGYFKYTNFLVNNINSVLGTELFF
HHHHHHHHHCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQVILPLGISFYTFTQIAFLVDCYKNLAKEYSFVNYALFVTFFPHLIAGPILHHKEMMSQ
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQSRWTLVIRHRNVVMGLFIFSIGLFKKVMIADTFAIWADAGFAQGVSHDFFSAWTTSLS
HHHCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHE
YTFQLYFDFSGYCDMAIGAALLFNIWLPLNFNSPYKALDIQDFWRRWHMTLSRYLRDYLY
EEEEEEEECCCCHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHEE
VPLGGNRCGRQRLYFNLMVTFVLGGLWHGASWMFIIWGALHGGAIVMHRIWKQVGLSLPD
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
PVGWFVTFMFVNITWVFFRADSVDDALRILHGMIDFDSIDQLSVNTVPTANLAWGGTLSD
CHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
RLLETLPTGLTANLAPTLMIAVALLIIRCKNAFELTAHTDFGKAKTLAMSTLFCLSLYAS
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
VQSKSPVFLYFNF
HCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]