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The map label for this gene is iscS [H]

Identifier: 77460833

GI number: 77460833

Start: 5211089

End: 5212303

Strand: Reverse

Name: iscS [H]

Synonym: Pfl01_4612

Alternate gene names: 77460833

Gene position: 5212303-5211089 (Counterclockwise)

Preceding gene: 77460834

Following gene: 77460832

Centisome position: 80.96

GC content: 59.34

Gene sequence:

>1215_bases
ATGAAATTGCCGATTTACCTTGATTACTCTGCGACCACCCCGGTCGATCCGCGCGTTGCGCAAAAAATGAGTGAATGCCT
GCTGGTCGACGGAAACTTCGGTAACCCGGCGTCCCGTTCCCACGTGTTCGGCTGGAAAGCCGAAGAGTCCGTCGAAAACG
CCCGTCGTCAGGTGGCCGATCTGGTCAACGCCGACCCGCGTGAAATCGTCTGGACCTCCGGTGCCACCGAGTCCGACAAC
CTGGCAATCAAGGGTGCGGCACATTTCTATGCCTCCAAGGGCAAGCACCTGATCACCTCCAAGATCGAACACAAGGCTGT
TCTCGACACCATGCGCCAGCTGGAGCGTGAAGGTTTCGAAGTCACCTACCTCGACCCGACCGAAGATGGCCTGATCACCC
CGGCCATGATCGAAGCCGCGCTGCGCGAAGACACCATCCTGGTGTCGGTAATGCACGTGAACAACGAAATCGGCACCGTC
AACGACATCGCCGCCATCGGCGAGTTGCTTCGTTCCAAAGGCATCCTGTTCCACGTTGACGCCGCTCAGTCCACTGGCAA
GGTCGAAATCGACCTGCAGAAACTGAAAGTCGACATGATGTCGTTCTCCGCCCACAAAACCTACGGCCCTAAAGGTATCG
GCGCGCTGTACGTTAGCCGCAAGCCGCGTGTGCGCATCGAAGCGACCATGCACGGTGGCGGTCACGAGCGTGGCATGCGT
TCTGGCACCCTGGCGACTCACCAGATCGTCGGCATGGGCGAAGCGTTCCGTGTGGCCAAGGAAGACATGGCTGCCGAAAA
CGTCCGTATCAAGGCATTGAGCGACCGCTTCTACAAGCAGGTCGAGCACCTGGAAGAGCTGTACGTCAACGGCAGCCTGA
CCGCCCGCGTTCCGCACAACCTGAACCTGAGCTTCAACTACGTTGAAGGCGAGTCGCTGATCATGGCGCTCAAGGATCTG
GCGGTTTCGTCCGGTTCGGCCTGCACCTCGGCGTCGCTTGAGCCTTCGTACGTACTGCGCGCCCTGGGCCGCAACGACGA
ACTGGCACACAGCTCGATCCGTTTCACCTTCGGCCGTTTCACCACCGAAGAAGAAATCGACTACGCCGCGCAGAAAGTCT
GCGAGGCCGTTACCAAGCTGCGCGCTCTGTCGCCGCTGTGGGACATGTACAAAGACGGCGTCGATATCTCGAAGATCGAG
TGGGCGGCACACTAA

Upstream 100 bases:

>100_bases
ATGGCAAGGCGCCGCGCCTGGACAAGATTGAAGCGTCCGCCGTCGAATGACAGCCAGAGAGCTAGCGGCACGCCAGCCAG
CCTGATTTAGGAGATAGTCC

Downstream 100 bases:

>100_bases
ATATAGAAGCCGCCGGATACAGATCCTGTAGCGACGCGGCGGTTTACGCAGCGCAGTTGCAGGGTCTCAAGAGCGGCCCT
GATGAGTGAGGATTGAGAAT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYSATTPVDPRVAQKMSECLLVDGNFGNPASRSHVFGWKAEESVENARRQVADLVNADPREIVWTSGATESDN
LAIKGAAHFYASKGKHLITSKIEHKAVLDTMRQLEREGFEVTYLDPTEDGLITPAMIEAALREDTILVSVMHVNNEIGTV
NDIAAIGELLRSKGILFHVDAAQSTGKVEIDLQKLKVDMMSFSAHKTYGPKGIGALYVSRKPRVRIEATMHGGGHERGMR
SGTLATHQIVGMGEAFRVAKEDMAAENVRIKALSDRFYKQVEHLEELYVNGSLTARVPHNLNLSFNYVEGESLIMALKDL
AVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRFTTEEEIDYAAQKVCEAVTKLRALSPLWDMYKDGVDISKIE
WAAH

Sequences:

>Translated_404_residues
MKLPIYLDYSATTPVDPRVAQKMSECLLVDGNFGNPASRSHVFGWKAEESVENARRQVADLVNADPREIVWTSGATESDN
LAIKGAAHFYASKGKHLITSKIEHKAVLDTMRQLEREGFEVTYLDPTEDGLITPAMIEAALREDTILVSVMHVNNEIGTV
NDIAAIGELLRSKGILFHVDAAQSTGKVEIDLQKLKVDMMSFSAHKTYGPKGIGALYVSRKPRVRIEATMHGGGHERGMR
SGTLATHQIVGMGEAFRVAKEDMAAENVRIKALSDRFYKQVEHLEELYVNGSLTARVPHNLNLSFNYVEGESLIMALKDL
AVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRFTTEEEIDYAAQKVCEAVTKLRALSPLWDMYKDGVDISKIE
WAAH
>Mature_404_residues
MKLPIYLDYSATTPVDPRVAQKMSECLLVDGNFGNPASRSHVFGWKAEESVENARRQVADLVNADPREIVWTSGATESDN
LAIKGAAHFYASKGKHLITSKIEHKAVLDTMRQLEREGFEVTYLDPTEDGLITPAMIEAALREDTILVSVMHVNNEIGTV
NDIAAIGELLRSKGILFHVDAAQSTGKVEIDLQKLKVDMMSFSAHKTYGPKGIGALYVSRKPRVRIEATMHGGGHERGMR
SGTLATHQIVGMGEAFRVAKEDMAAENVRIKALSDRFYKQVEHLEELYVNGSLTARVPHNLNLSFNYVEGESLIMALKDL
AVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRFTTEEEIDYAAQKVCEAVTKLRALSPLWDMYKDGVDISKIE
WAAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=55.7213930348259, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=31.6425120772947, Blast_Score=185, Evalue=5e-47,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=73.7623762376238, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=224, Percent_Identity=32.5892857142857, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1789175, Length=168, Percent_Identity=32.7380952380952, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=55.3349875930521, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=32.4242424242424, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=54.1147132169576, Blast_Score=458, Evalue=1e-130,
Organism=Drosophila melanogaster, GI20129463, Length=403, Percent_Identity=53.5980148883375, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44599; Mature: 44599

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYSATTPVDPRVAQKMSECLLVDGNFGNPASRSHVFGWKAEESVENARRQVAD
CCCEEEEECCCCCCCCHHHHHHHHCEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHH
LVNADPREIVWTSGATESDNLAIKGAAHFYASKGKHLITSKIEHKAVLDTMRQLEREGFE
HHCCCCCCEEEECCCCCCCCEEEEEHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCE
VTYLDPTEDGLITPAMIEAALREDTILVSVMHVNNEIGTVNDIAAIGELLRSKGILFHVD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEE
AAQSTGKVEIDLQKLKVDMMSFSAHKTYGPKGIGALYVSRKPRVRIEATMHGGGHERGMR
CCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCC
SGTLATHQIVGMGEAFRVAKEDMAAENVRIKALSDRFYKQVEHLEELYVNGSLTARVPHN
CCCCHHHHHHCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCC
LNLSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCEEEEEECCC
TTEEEIDYAAQKVCEAVTKLRALSPLWDMYKDGVDISKIEWAAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC
>Mature Secondary Structure
MKLPIYLDYSATTPVDPRVAQKMSECLLVDGNFGNPASRSHVFGWKAEESVENARRQVAD
CCCEEEEECCCCCCCCHHHHHHHHCEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHHHH
LVNADPREIVWTSGATESDNLAIKGAAHFYASKGKHLITSKIEHKAVLDTMRQLEREGFE
HHCCCCCCEEEECCCCCCCCEEEEEHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCE
VTYLDPTEDGLITPAMIEAALREDTILVSVMHVNNEIGTVNDIAAIGELLRSKGILFHVD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEE
AAQSTGKVEIDLQKLKVDMMSFSAHKTYGPKGIGALYVSRKPRVRIEATMHGGGHERGMR
CCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCC
SGTLATHQIVGMGEAFRVAKEDMAAENVRIKALSDRFYKQVEHLEELYVNGSLTARVPHN
CCCCHHHHHHCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEECCCC
LNLSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCEEEEEECCC
TTEEEIDYAAQKVCEAVTKLRALSPLWDMYKDGVDISKIEWAAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA