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The map label for this gene is hisS

Identifier: 77460821

GI number: 77460821

Start: 5201295

End: 5202584

Strand: Reverse

Name: hisS

Synonym: Pfl01_4600

Alternate gene names: 77460821

Gene position: 5202584-5201295 (Counterclockwise)

Preceding gene: 77460822

Following gene: 77460820

Centisome position: 80.81

GC content: 60.39

Gene sequence:

>1290_bases
GTGAGCAAGTCTCTGCAAGCCATTCGTGGCATGAACGACATCCTGCCGGAACAGACGCCGGTATGGCGTTATTTCGAAGG
CACCGTTTCGCGTCTGCTGGATAATTACGGTTACAAGCAGATCCGCATGCCAATCGTCGAGTTCACCGAGCTGTTCAAAC
GCTCGATCGGTGAAGTGACCGACATCGTCGAAAAAGAGATGTACACCTTCGAAGACCGCAACGGCGACTCGCTGACCCTG
CGTCCGGAAGGCACGGCCGCGTGCGTACGTGCGGTGCTCGAACACGGCATCACCGGCGGTGGCCAGGTACAGAAACTCTG
GTACATCGGCCCGATGTTCCGTCACGAGCGTCCACAGAAAGGCCGTTATCGCCAGTTCCACCAGATCGGTTTGGAGGTGT
TCAACCTCGACGGTCCGGACATCGACGCCGAACTGATCATCATGACCTGGCGCCTGTGGGGCGAGCTGGGCATCCGCGAT
GCGGTCAAGCTTGAACTCAACAGCCTCGGCACCAGCGAATCCCGCGGCCGTTATCGTGAAGCGCTGGTCGAGTACCTCTC
GGCGCACCACGACAAACTGGACGAAGACAGCCAGCGTCGCCTGAAAACCAACCCGCTGCGCGTGCTCGACACCAAGAACG
CCGACACTCAAGCGGTGTTGGTCGATGCGCCGAAAATGGCCGACTACCTGGATGACGAATCCCGTGCCCACTTCAAAGGC
CTGAAGGCCCGTCTGGATGCCGTCGGTATTCCTTACGTGCTGAACCCGAAGCTGGTGCGTGGTCTGGATTACTACAGCAA
AACCGTATTCGAATGGGTCACCGACAAGCTCGGCGCCCAGGGCACCGTTTGCGCGGGTGGGCGCTATGACGGTCTGGTCG
AGCAGATGGGCGGCAAGCCGACCCCGGGCGTCGGTTTCGCCATGGGCATTGAGCGTCTGGTACTGCTACTGGAAACCCTG
GAGCAGATCCCGGAAGAAATCTCCCGTCAGGTCGACGTCTATCTCTGCGCCTTCGGCGAAGAAGCAGAGCTGGCCGGTCT
GGCCCTGGCCGAGCGTGTACGCGACCAACTGCCCAACCTGCGCCTGCAAGTCAATGCCGGCGCCGGCAGCTTCAAGAGCC
AGTTCAAGAAAGCCGACAAGAGCGGTGCGCTGTACGCACTGATCCTCGGTGACGACGAAATGGCCCAGCAAGTGGTAGGT
TTCAAACCCCTGCGTGGCCAGGGCGAACAACAAAGCATTGCCTGGGATGCGCTCGCTGCACACCTGGCCACCTGCGTCGT
GCAGGGTTGA

Upstream 100 bases:

>100_bases
GACGAGCTGGAAAAGCTGATCCGCGAGAAAGCGGCCGAGAAGGTCGAAGCTGACGCAGCGGTTATCGCGCGCGGCTGATC
GAACGAATTAAGGAATTTCT

Downstream 100 bases:

>100_bases
AGCTGTCCAAACAGCCGATTTAGCGATTAAGGAGTATTGGGGTGTCGAGTACCGAAGACGAACAGTTGGCGGATTTGAAG
GACTGGTGGACACGCAACGG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSKSLQAIRGMNDILPEQTPVWRYFEGTVSRLLDNYGYKQIRMPIVEFTELFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQKGRYRQFHQIGLEVFNLDGPDIDAELIIMTWRLWGELGIRD
AVKLELNSLGTSESRGRYREALVEYLSAHHDKLDEDSQRRLKTNPLRVLDTKNADTQAVLVDAPKMADYLDDESRAHFKG
LKARLDAVGIPYVLNPKLVRGLDYYSKTVFEWVTDKLGAQGTVCAGGRYDGLVEQMGGKPTPGVGFAMGIERLVLLLETL
EQIPEEISRQVDVYLCAFGEEAELAGLALAERVRDQLPNLRLQVNAGAGSFKSQFKKADKSGALYALILGDDEMAQQVVG
FKPLRGQGEQQSIAWDALAAHLATCVVQG

Sequences:

>Translated_429_residues
MSKSLQAIRGMNDILPEQTPVWRYFEGTVSRLLDNYGYKQIRMPIVEFTELFKRSIGEVTDIVEKEMYTFEDRNGDSLTL
RPEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQKGRYRQFHQIGLEVFNLDGPDIDAELIIMTWRLWGELGIRD
AVKLELNSLGTSESRGRYREALVEYLSAHHDKLDEDSQRRLKTNPLRVLDTKNADTQAVLVDAPKMADYLDDESRAHFKG
LKARLDAVGIPYVLNPKLVRGLDYYSKTVFEWVTDKLGAQGTVCAGGRYDGLVEQMGGKPTPGVGFAMGIERLVLLLETL
EQIPEEISRQVDVYLCAFGEEAELAGLALAERVRDQLPNLRLQVNAGAGSFKSQFKKADKSGALYALILGDDEMAQQVVG
FKPLRGQGEQQSIAWDALAAHLATCVVQG
>Mature_428_residues
SKSLQAIRGMNDILPEQTPVWRYFEGTVSRLLDNYGYKQIRMPIVEFTELFKRSIGEVTDIVEKEMYTFEDRNGDSLTLR
PEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQKGRYRQFHQIGLEVFNLDGPDIDAELIIMTWRLWGELGIRDA
VKLELNSLGTSESRGRYREALVEYLSAHHDKLDEDSQRRLKTNPLRVLDTKNADTQAVLVDAPKMADYLDDESRAHFKGL
KARLDAVGIPYVLNPKLVRGLDYYSKTVFEWVTDKLGAQGTVCAGGRYDGLVEQMGGKPTPGVGFAMGIERLVLLLETLE
QIPEEISRQVDVYLCAFGEEAELAGLALAERVRDQLPNLRLQVNAGAGSFKSQFKKADKSGALYALILGDDEMAQQVVGF
KPLRGQGEQQSIAWDALAAHLATCVVQG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=427, Percent_Identity=62.5292740046838, Blast_Score=548, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_PSEPF (Q3K7B7)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_350328.1
- ProteinModelPortal:   Q3K7B7
- SMR:   Q3K7B7
- STRING:   Q3K7B7
- GeneID:   3713599
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_4600
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   PFLU205922:PFL_4600-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47997; Mature: 47866

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLQAIRGMNDILPEQTPVWRYFEGTVSRLLDNYGYKQIRMPIVEFTELFKRSIGEVT
CCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQK
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCC
GRYRQFHQIGLEVFNLDGPDIDAELIIMTWRLWGELGIRDAVKLELNSLGTSESRGRYRE
CHHHHHHHHCCEEEECCCCCCCCEEEEEEHHHHHHCCCCHHHEEEHHHCCCCCHHHHHHH
ALVEYLSAHHDKLDEDSQRRLKTNPLRVLDTKNADTQAVLVDAPKMADYLDDESRAHFKG
HHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEECCHHHHHCCCCHHHHHHH
LKARLDAVGIPYVLNPKLVRGLDYYSKTVFEWVTDKLGAQGTVCAGGRYDGLVEQMGGKP
HHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCC
TPGVGFAMGIERLVLLLETLEQIPEEISRQVDVYLCAFGEEAELAGLALAERVRDQLPNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHCCCE
RLQVNAGAGSFKSQFKKADKSGALYALILGDDEMAQQVVGFKPLRGQGEQQSIAWDALAA
EEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
HLATCVVQG
HHHHHHCCC
>Mature Secondary Structure 
SKSLQAIRGMNDILPEQTPVWRYFEGTVSRLLDNYGYKQIRMPIVEFTELFKRSIGEVT
CCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
DIVEKEMYTFEDRNGDSLTLRPEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQK
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCC
GRYRQFHQIGLEVFNLDGPDIDAELIIMTWRLWGELGIRDAVKLELNSLGTSESRGRYRE
CHHHHHHHHCCEEEECCCCCCCCEEEEEEHHHHHHCCCCHHHEEEHHHCCCCCHHHHHHH
ALVEYLSAHHDKLDEDSQRRLKTNPLRVLDTKNADTQAVLVDAPKMADYLDDESRAHFKG
HHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEECCHHHHHCCCCHHHHHHH
LKARLDAVGIPYVLNPKLVRGLDYYSKTVFEWVTDKLGAQGTVCAGGRYDGLVEQMGGKP
HHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHCCCC
TPGVGFAMGIERLVLLLETLEQIPEEISRQVDVYLCAFGEEAELAGLALAERVRDQLPNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHCCCE
RLQVNAGAGSFKSQFKKADKSGALYALILGDDEMAQQVVGFKPLRGQGEQQSIAWDALAA
EEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
HLATCVVQG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA