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The map label for this gene is yafV [H]

Identifier: 77460816

GI number: 77460816

Start: 5195812

End: 5196594

Strand: Reverse

Name: yafV [H]

Synonym: Pfl01_4595

Alternate gene names: 77460816

Gene position: 5196594-5195812 (Counterclockwise)

Preceding gene: 77460817

Following gene: 77460811

Centisome position: 80.71

GC content: 63.6

Gene sequence:

>783_bases
TTGAGTGCGTTGCCCGATCTCAATCTGGCGCTGATCCAGACCAGCCTGGCCTGGCATGACCGTCAGGCCAACCTCGAGCA
TTTCGACGCCTTGCTGGAGCAGGCGCGCGGTGCGGATCTGATCATCTTGCCGGAAATGTTCACCACCGGATTCTCCATGG
CGTCCGAAACCCTGGCCGAATCCGAAAACGGCCCGACCAGCAAATGGCTGCGGATCCAGGCGGCGAAACTGAACGCGGTC
GTCACCGGCAGCGTGATCATCCAGGCCGCCGACGGCAGTCATCGCAACCGCTTGCTGTGGGCGCGGCCGGACGGCGAAGT
GCTGCATTACGATAAACGTCACCTGTTCCGCATGGCCGGCGAGCACAATCACTACACCCCCGGCGAGCGTCAGGTGCAGT
TCGAGCTCAAGGGCTGGCGGGTGCGTCCGCTGATTTGCTACGACTTGCGCTTCCCGGTGTGGAGCCGCGACGCTCAGGAC
ACCGATCTGTTGCTGTACACGGCCAACTGGCCGGGCGCGCGGCGTCAGCACTGGAACCGCTTGCTGCCGGCCCGGGCCAT
CGAAAACCTGTGCTATGTGGCGGCGGTCAACCGGGTTGGCACCGATGGCAAGGGCTTTGCCTATACCGGCGACAGTCAGG
TGCTGGATTTTCAGGGCGAGACGTTGCTGGCGGCGGGAGAGGCTGACGGGGTGTTCAAGGTGGTTCTGGAAGCGGCTTCA
CTGGCGGCTTACCGCGAGCGGTTCCCGGCGAATCTGGATGCGGACACCTTCGAGTTCACCTGA

Upstream 100 bases:

>100_bases
TGTTCTACCAGCATCCACCCGAAGGCCAGCGCTTGGTGCGCCTGTGCTTTGCCAAACGTGAGGAGACGCTGCGGGAAGCG
GCGGCCAAACTATGCGTGAT

Downstream 100 bases:

>100_bases
TAGTCACGAGGCGCCTGATCGTTCCCATGCTCTGCGTGGGAACGATCAGAAGAAGCAATAAAAAGGCCCCGAGGTGCAAA
CCCCGGGGCCTTTTGCATTG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSALPDLNLALIQTSLAWHDRQANLEHFDALLEQARGADLIILPEMFTTGFSMASETLAESENGPTSKWLRIQAAKLNAV
VTGSVIIQAADGSHRNRLLWARPDGEVLHYDKRHLFRMAGEHNHYTPGERQVQFELKGWRVRPLICYDLRFPVWSRDAQD
TDLLLYTANWPGARRQHWNRLLPARAIENLCYVAAVNRVGTDGKGFAYTGDSQVLDFQGETLLAAGEADGVFKVVLEAAS
LAAYRERFPANLDADTFEFT

Sequences:

>Translated_260_residues
MSALPDLNLALIQTSLAWHDRQANLEHFDALLEQARGADLIILPEMFTTGFSMASETLAESENGPTSKWLRIQAAKLNAV
VTGSVIIQAADGSHRNRLLWARPDGEVLHYDKRHLFRMAGEHNHYTPGERQVQFELKGWRVRPLICYDLRFPVWSRDAQD
TDLLLYTANWPGARRQHWNRLLPARAIENLCYVAAVNRVGTDGKGFAYTGDSQVLDFQGETLLAAGEADGVFKVVLEAAS
LAAYRERFPANLDADTFEFT
>Mature_259_residues
SALPDLNLALIQTSLAWHDRQANLEHFDALLEQARGADLIILPEMFTTGFSMASETLAESENGPTSKWLRIQAAKLNAVV
TGSVIIQAADGSHRNRLLWARPDGEVLHYDKRHLFRMAGEHNHYTPGERQVQFELKGWRVRPLICYDLRFPVWSRDAQDT
DLLLYTANWPGARRQHWNRLLPARAIENLCYVAAVNRVGTDGKGFAYTGDSQVLDFQGETLLAAGEADGVFKVVLEAASL
AAYRERFPANLDADTFEFT

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=264, Percent_Identity=26.8939393939394, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1786412, Length=260, Percent_Identity=50.7692307692308, Blast_Score=251, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6323383, Length=274, Percent_Identity=24.0875912408759, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29212; Mature: 29081

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALPDLNLALIQTSLAWHDRQANLEHFDALLEQARGADLIILPEMFTTGFSMASETLAE
CCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
SENGPTSKWLRIQAAKLNAVVTGSVIIQAADGSHRNRLLWARPDGEVLHYDKRHLFRMAG
CCCCCCCCEEEEEEEEEEEEEEEEEEEEECCCCCCCCEEEECCCCCEEEECHHHHHHHCC
EHNHYTPGERQVQFELKGWRVRPLICYDLRFPVWSRDAQDTDLLLYTANWPGARRQHWNR
CCCCCCCCCEEEEEEECCEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHH
LLPARAIENLCYVAAVNRVGTDGKGFAYTGDSQVLDFQGETLLAAGEADGVFKVVLEAAS
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCEEEEECCCCCHHHHHHHHHH
LAAYRERFPANLDADTFEFT
HHHHHHHCCCCCCCCCEECC
>Mature Secondary Structure 
SALPDLNLALIQTSLAWHDRQANLEHFDALLEQARGADLIILPEMFTTGFSMASETLAE
CCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
SENGPTSKWLRIQAAKLNAVVTGSVIIQAADGSHRNRLLWARPDGEVLHYDKRHLFRMAG
CCCCCCCCEEEEEEEEEEEEEEEEEEEEECCCCCCCCEEEECCCCCEEEECHHHHHHHCC
EHNHYTPGERQVQFELKGWRVRPLICYDLRFPVWSRDAQDTDLLLYTANWPGARRQHWNR
CCCCCCCCCEEEEEEECCEEEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHH
LLPARAIENLCYVAAVNRVGTDGKGFAYTGDSQVLDFQGETLLAAGEADGVFKVVLEAAS
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCEEEEECCCCCHHHHHHHHHH
LAAYRERFPANLDADTFEFT
HHHHHHHCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]