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The map label for this gene is ramA [H]

Identifier: 77460778

GI number: 77460778

Start: 5140991

End: 5141812

Strand: Reverse

Name: ramA [H]

Synonym: Pfl01_4557

Alternate gene names: 77460778

Gene position: 5141812-5140991 (Counterclockwise)

Preceding gene: 77460781

Following gene: 77460777

Centisome position: 79.86

GC content: 64.6

Gene sequence:

>822_bases
ATGAAAGTCGAACTCGCCCAGTTGGCGGGCCGTGACAAAGACACGGCGTACAACCTCGAACGCGCCCTCGCGGCGATGGC
GGCCTGCGCCGCCGACACGCAATTGATCGTGTTCCCCGAGACCCACCTGATGGGCTTTCCGACCGCCGACACCGTGGCGC
AGATCGCCGAACCGCTGGACGGCCCGACCGTCAGCACCGTGCAGGCTGCCGCCCGTGAGCGCAACATTGCCGTGGTGATC
GGCATGGCCGAAAACGACAACGGCCGCTACTACAACACCACGCTGCTGATTACTCCCGAAGGCATTGCCCTGAAATACCG
CAAGACCCACCTCTGGGCCTCTGATCGCGGTGTGTTCGAGGCAGGCGACCGTTACGCCACCTGCCTGTGGAACGGCGTGC
GCGTCGGTCTGCTGATCTGCTACGACATTGAGTTCCCCGAGTCGGCCCGCGCCCTGGCGCAACTGGGCGCCGAATTGCTG
ATCGTGACCAACGGCAACATGGACCCCTACGGCCCGACCCACCGCACCGCGATCATGGCCCGGGCCCAGGAAAACCAGGC
GTTTGCGCTGATGGTCAACCGGGTGGAAGCCGGGGATGACGGGTTGATGTTTGCCGGCGGCAGTGCGCTGGTGGATCCGC
TGGGCACGCTGCTGTTCGAAGCCGGGCGTGAGGAAGGGCGGTTCAGTGTCGAGCTGGATTTCGGCCAGCTGGAGCTGGCA
CGCAAGGATTATCGGTATCTGGATGATCAGCGGTTGAGGTTGCCGGGGGAGGTGGTTGAACACGCCTGCGGAGTACGTGA
GCTGCTGATCCCCTCGCGCTGA

Upstream 100 bases:

>100_bases
GGCCGCGTAATTCCCGGGCCGGTCGAATGCGAAGCATACTGGCCCACAGACAGCCGCCGGGCAATCTTTCGGCGCCCGGC
GCTCATAACAAGGACTACCC

Downstream 100 bases:

>100_bases
TCCTGATCCTGTGGCGCCACGGCTGGCGCCATCGCGAGCAAGCTCGCTCCCACAGGGGAACGCGGTCACTGTTGGAGCGA
GCTTGCTCGCGATGGGGCCC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKVELAQLAGRDKDTAYNLERALAAMAACAADTQLIVFPETHLMGFPTADTVAQIAEPLDGPTVSTVQAAARERNIAVVI
GMAENDNGRYYNTTLLITPEGIALKYRKTHLWASDRGVFEAGDRYATCLWNGVRVGLLICYDIEFPESARALAQLGAELL
IVTNGNMDPYGPTHRTAIMARAQENQAFALMVNRVEAGDDGLMFAGGSALVDPLGTLLFEAGREEGRFSVELDFGQLELA
RKDYRYLDDQRLRLPGEVVEHACGVRELLIPSR

Sequences:

>Translated_273_residues
MKVELAQLAGRDKDTAYNLERALAAMAACAADTQLIVFPETHLMGFPTADTVAQIAEPLDGPTVSTVQAAARERNIAVVI
GMAENDNGRYYNTTLLITPEGIALKYRKTHLWASDRGVFEAGDRYATCLWNGVRVGLLICYDIEFPESARALAQLGAELL
IVTNGNMDPYGPTHRTAIMARAQENQAFALMVNRVEAGDDGLMFAGGSALVDPLGTLLFEAGREEGRFSVELDFGQLELA
RKDYRYLDDQRLRLPGEVVEHACGVRELLIPSR
>Mature_273_residues
MKVELAQLAGRDKDTAYNLERALAAMAACAADTQLIVFPETHLMGFPTADTVAQIAEPLDGPTVSTVQAAARERNIAVVI
GMAENDNGRYYNTTLLITPEGIALKYRKTHLWASDRGVFEAGDRYATCLWNGVRVGLLICYDIEFPESARALAQLGAELL
IVTNGNMDPYGPTHRTAIMARAQENQAFALMVNRVEAGDDGLMFAGGSALVDPLGTLLFEAGREEGRFSVELDFGQLELA
RKDYRYLDDQRLRLPGEVVEHACGVRELLIPSR

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=250, Percent_Identity=28.4, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1786412, Length=219, Percent_Identity=27.8538812785388, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=281, Percent_Identity=27.7580071174377, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355835, Length=272, Percent_Identity=27.5735294117647, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 29878; Mature: 29878

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVELAQLAGRDKDTAYNLERALAAMAACAADTQLIVFPETHLMGFPTADTVAQIAEPLD
CCEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHEECCCCHHHHHHHHCCCC
GPTVSTVQAAARERNIAVVIGMAENDNGRYYNTTLLITPEGIALKYRKTHLWASDRGVFE
CCCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCEEEEEEEEEEEECCCCCCC
AGDRYATCLWNGVRVGLLICYDIEFPESARALAQLGAELLIVTNGNMDPYGPTHRTAIMA
CCCCEEEEEECCCEEEEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHEEEE
RAQENQAFALMVNRVEAGDDGLMFAGGSALVDPLGTLLFEAGREEGRFSVELDFGQLELA
ECCCCCEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHH
RKDYRYLDDQRLRLPGEVVEHACGVRELLIPSR
HHHHHHCCCCCCCCCHHHHHHHCCHHHHCCCCC
>Mature Secondary Structure
MKVELAQLAGRDKDTAYNLERALAAMAACAADTQLIVFPETHLMGFPTADTVAQIAEPLD
CCEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHEECCCCHHHHHHHHCCCC
GPTVSTVQAAARERNIAVVIGMAENDNGRYYNTTLLITPEGIALKYRKTHLWASDRGVFE
CCCHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCEEEEEEEEEEEECCCCCCC
AGDRYATCLWNGVRVGLLICYDIEFPESARALAQLGAELLIVTNGNMDPYGPTHRTAIMA
CCCCEEEEEECCCEEEEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHEEEE
RAQENQAFALMVNRVEAGDDGLMFAGGSALVDPLGTLLFEAGREEGRFSVELDFGQLELA
ECCCCCEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHH
RKDYRYLDDQRLRLPGEVVEHACGVRELLIPSR
HHHHHHCCCCCCCCCHHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA