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The map label for this gene is hupR1 [H]

Identifier: 77460771

GI number: 77460771

Start: 5129543

End: 5130898

Strand: Reverse

Name: hupR1 [H]

Synonym: Pfl01_4550

Alternate gene names: 77460771

Gene position: 5130898-5129543 (Counterclockwise)

Preceding gene: 77460777

Following gene: 77460770

Centisome position: 79.69

GC content: 61.14

Gene sequence:

>1356_bases
ATGGAAGAGCCGTCCGCCCCGATTTCGCCGCCCCGACCCAAAGTGCTGCTGGTCGATGACGAGGAACCGATCCTCAACAG
CCTGCGCCGTCTGCTGCGCGGCCAGCCTTATGACGTGTTGCTGGCCACCAGCGGTGCCCAGGCTCTGGAAATCCTCGAGC
AGCAACCGGTGAATCTGGTGATGAGCGATGCGCGCATGCCCAATATGGATGGCGCCACGCTGCTGGCTCGCGTTCATGAG
CTGTACCCGGCGACCGCGCGGATCATGCTCACCGGCTACGCCGAACCTTCTGCAATCATCAAGGCCATCAACGAAGGGCA
GATCGAGCGCTACATCAGCAAACCCTGGAACGACGAAGAAATGCTCCTGACCTTGCGTCAGGCCCTCGAGCATCAGCATC
TGGCACGCGAGCGCGAGCGTCTGGAACAACTGACGCGAGCGCAAAACGATCAGCTGAAGTTGCTCAACAGCACTCTGGAA
AAGCGCGTCGCCGCCCGCACCGCCGAGCTGCAGCAGACCGCCGACATGCTCGATCTGGCCTACGAGGAGCTCAAGCGCAG
TTTCCTGACAGGCACTGAAATGTTCTCGTTGCTGGCCAACCTGCGCCTGCCGCCGACCAAGCAGACCAACCGGCAGATCA
TCGAACTGGTGCGCTGCTATTGCCGCGAACATCGGTTTGATGAAGCCGCCAGCCGCGACCTGACCATGGCCGCCGCGCTC
TACAACATCGGCAAGCTGAGCTGGACTGACAGCATGATGACCACGCCCTCGGACCTGCTGCACTCCACAGAGCGCGACCG
CTACCGTGCCTATCCGAAACAGAGCGAATCGCTATTGATGACCCTCGACCCGATGAAGGACGCGGCGCGGCTGATCCTGC
ACCATCAGGAACACTGGGACGGCACAGGCTTCCCGGACCGGCTCAAGGGCGAGGCCATTCCCCTGGGCTCGCGGCTGTTG
AAACTGGCGGTGGATTTCGTCGAGTTGCAGCGCGGGCTGATTCTCGAACGGCAGATGAACAGCGATGAAGCGCTGTTGTA
CCTGCGCCAGTACGCCGGCAAAGTCTATGATCCTGAGCTGATCGAGGACTTCGTCAAGGTGTGTGCGACGGTCAGCGATA
TCACGCTGATCGATCCGAGCGTCAGGGTGCTGGGCACCCGGGAGCTGGCAGCGGGGATGATCCTGGCGCGCAATCTCAAC
GCCGACAACGGCATGTTGCTGCTCAATGCCGGCAAGGTGCTGAGTGCTTTGCTGGTGGACAAACTGATCAGTTTCGAAGC
CATGGAGGGCGCTAAATACAAGGTGTTCGTGAAAGTACCGGAGGAGGGGGAAAGCGTGCTTCTGGCCCAGGGGTGA

Upstream 100 bases:

>100_bases
GGCCCCGGCGAAAATCATCTAGCCTTTGGTTCAGGCGCGGGCAAGCGGGGAGGTCTGTCGGCGCGTCAGTCATGTCCAAC
GTTTTTCACGAGGCTTCGCC

Downstream 100 bases:

>100_bases
TGACGCTGACGCGACCGGCATGGGATCCTTGCGGTTTTTCTCAAGGCCGGTGGCTGCCCCATGACCTCTGTATCCGCTGT
TTCCCCTCGCATCATTCGTA

Product: response regulator receiver domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MEEPSAPISPPRPKVLLVDDEEPILNSLRRLLRGQPYDVLLATSGAQALEILEQQPVNLVMSDARMPNMDGATLLARVHE
LYPATARIMLTGYAEPSAIIKAINEGQIERYISKPWNDEEMLLTLRQALEHQHLARERERLEQLTRAQNDQLKLLNSTLE
KRVAARTAELQQTADMLDLAYEELKRSFLTGTEMFSLLANLRLPPTKQTNRQIIELVRCYCREHRFDEAASRDLTMAAAL
YNIGKLSWTDSMMTTPSDLLHSTERDRYRAYPKQSESLLMTLDPMKDAARLILHHQEHWDGTGFPDRLKGEAIPLGSRLL
KLAVDFVELQRGLILERQMNSDEALLYLRQYAGKVYDPELIEDFVKVCATVSDITLIDPSVRVLGTRELAAGMILARNLN
ADNGMLLLNAGKVLSALLVDKLISFEAMEGAKYKVFVKVPEEGESVLLAQG

Sequences:

>Translated_451_residues
MEEPSAPISPPRPKVLLVDDEEPILNSLRRLLRGQPYDVLLATSGAQALEILEQQPVNLVMSDARMPNMDGATLLARVHE
LYPATARIMLTGYAEPSAIIKAINEGQIERYISKPWNDEEMLLTLRQALEHQHLARERERLEQLTRAQNDQLKLLNSTLE
KRVAARTAELQQTADMLDLAYEELKRSFLTGTEMFSLLANLRLPPTKQTNRQIIELVRCYCREHRFDEAASRDLTMAAAL
YNIGKLSWTDSMMTTPSDLLHSTERDRYRAYPKQSESLLMTLDPMKDAARLILHHQEHWDGTGFPDRLKGEAIPLGSRLL
KLAVDFVELQRGLILERQMNSDEALLYLRQYAGKVYDPELIEDFVKVCATVSDITLIDPSVRVLGTRELAAGMILARNLN
ADNGMLLLNAGKVLSALLVDKLISFEAMEGAKYKVFVKVPEEGESVLLAQG
>Mature_451_residues
MEEPSAPISPPRPKVLLVDDEEPILNSLRRLLRGQPYDVLLATSGAQALEILEQQPVNLVMSDARMPNMDGATLLARVHE
LYPATARIMLTGYAEPSAIIKAINEGQIERYISKPWNDEEMLLTLRQALEHQHLARERERLEQLTRAQNDQLKLLNSTLE
KRVAARTAELQQTADMLDLAYEELKRSFLTGTEMFSLLANLRLPPTKQTNRQIIELVRCYCREHRFDEAASRDLTMAAAL
YNIGKLSWTDSMMTTPSDLLHSTERDRYRAYPKQSESLLMTLDPMKDAARLILHHQEHWDGTGFPDRLKGEAIPLGSRLL
KLAVDFVELQRGLILERQMNSDEALLYLRQYAGKVYDPELIEDFVKVCATVSDITLIDPSVRVLGTRELAAGMILARNLN
ADNGMLLLNAGKVLSALLVDKLISFEAMEGAKYKVFVKVPEEGESVLLAQG

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=119, Percent_Identity=37.8151260504202, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790299, Length=120, Percent_Identity=30.8333333333333, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788905, Length=134, Percent_Identity=33.5820895522388, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=30, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50902; Mature: 50902

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEPSAPISPPRPKVLLVDDEEPILNSLRRLLRGQPYDVLLATSGAQALEILEQQPVNLV
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEE
MSDARMPNMDGATLLARVHELYPATARIMLTGYAEPSAIIKAINEGQIERYISKPWNDEE
ECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHH
MLLTLRQALEHQHLARERERLEQLTRAQNDQLKLLNSTLEKRVAARTAELQQTADMLDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEELKRSFLTGTEMFSLLANLRLPPTKQTNRQIIELVRCYCREHRFDEAASRDLTMAAAL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHH
YNIGKLSWTDSMMTTPSDLLHSTERDRYRAYPKQSESLLMTLDPMKDAARLILHHQEHWD
HHHCCCCCCCCHHCCCHHHHHCCHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCC
GTGFPDRLKGEAIPLGSRLLKLAVDFVELQRGLILERQMNSDEALLYLRQYAGKVYDPEL
CCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHCCCCCCHHH
IEDFVKVCATVSDITLIDPSVRVLGTRELAAGMILARNLNADNGMLLLNAGKVLSALLVD
HHHHHHHHHHHHCCEEECCCHHEEEHHHHHHHHHHEECCCCCCCEEEEEHHHHHHHHHHH
KLISFEAMEGAKYKVFVKVPEEGESVLLAQG
HHHHHHHCCCCEEEEEEEECCCCCEEEEECC
>Mature Secondary Structure
MEEPSAPISPPRPKVLLVDDEEPILNSLRRLLRGQPYDVLLATSGAQALEILEQQPVNLV
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEE
MSDARMPNMDGATLLARVHELYPATARIMLTGYAEPSAIIKAINEGQIERYISKPWNDEE
ECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHH
MLLTLRQALEHQHLARERERLEQLTRAQNDQLKLLNSTLEKRVAARTAELQQTADMLDLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEELKRSFLTGTEMFSLLANLRLPPTKQTNRQIIELVRCYCREHRFDEAASRDLTMAAAL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHH
YNIGKLSWTDSMMTTPSDLLHSTERDRYRAYPKQSESLLMTLDPMKDAARLILHHQEHWD
HHHCCCCCCCCHHCCCHHHHHCCHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCC
GTGFPDRLKGEAIPLGSRLLKLAVDFVELQRGLILERQMNSDEALLYLRQYAGKVYDPEL
CCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHCCCCCCHHH
IEDFVKVCATVSDITLIDPSVRVLGTRELAAGMILARNLNADNGMLLLNAGKVLSALLVD
HHHHHHHHHHHHCCEEECCCHHEEEHHHHHHHHHHEECCCCCCCEEEEEHHHHHHHHHHH
KLISFEAMEGAKYKVFVKVPEEGESVLLAQG
HHHHHHHCCCCEEEEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]