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The map label for this gene is iciA [H]

Identifier: 77460704

GI number: 77460704

Start: 5055133

End: 5056026

Strand: Reverse

Name: iciA [H]

Synonym: Pfl01_4483

Alternate gene names: 77460704

Gene position: 5056026-5055133 (Counterclockwise)

Preceding gene: 77460715

Following gene: 77460703

Centisome position: 78.53

GC content: 64.54

Gene sequence:

>894_bases
ATGTTCGACTACAAATTGCTTTCCGCTCTGGCCGCCGTGGTCGAGCAGGCCGGCTTCGAACGGGCGGCGCAGGTGCTGGG
CCTTTCGCAATCGGCGATATCCCAGCGGATCAAACTGCTGGAAGCACGGGTCGGCCAGCCGGTTTTGGTGCGCGGCACAC
CGCCGTCGCCGACCGAAATCGGCCGGCGCCTGCTCAACCATGTGCAGCAGGTGCGTCTGCTTGAGCGGGATTTGCAGACG
CTGGTGCCGGCGCTGGACGAAGAGGGCCTGCCGGAACGCCTGCGCATCGCCCTGAATGCCGACAGCCTCGCCACCTGGTG
GGCCGAAGCGGTGGGTGATTTCTGCGCCGAACAACATCTGTTGCTCGATCTGATCGTCGAAGACCAGACCGTCGGCCTCA
AGCGCATGCGCGCCGGTGAAGTGGCCGGTTGCCTGTGCGCCAGCGAACGGCCGGTAGCGGGTGCACGCAGCGTGCTGCTC
GGAGCGATGCGCTATCGGGCGCTGGCCAGCCCGGCGTTCATCAAGCGGCATTTTCCCGACGGCGTGCGGGCCGAACAATT
GCCGCGCACCCCCGCATTGGTCTTCGGCCCGGACGATTTTCTCCAGCATCGATACCTCGCGTCCCTCGGCGTCGATGGCG
GTTTCGAACATCACCTGTGCCCGTCGTCCGAAGGTTTTATTCGCCTGACCGAAGCCGGGCTCGGCTGGGGACTGGTGCCG
GAATTACAGGTGCGCGAGCAACTGGAGCGCGGCGTACTGCGCGAACTGTTGCCAGATAAACCGATCGATGTGCCCTTGTA
CTGGCATCATTGGCGCAATGGCGGTCAGTTGCTCGGGCTGTTGACCGAGCAATTGGTGCGCTCGTCGGCGCAATGGCTGG
TGCCGTTGGACTGA

Upstream 100 bases:

>100_bases
CCATTGTGGGAGTCCTTGATAAAGAGTGCTTGCTGGCTATTCTCCCGGTTGACGCTGTATAAGAAAAACCAATAGTGCTG
ATCGCTCATTAGGAAAACTG

Downstream 100 bases:

>100_bases
CGGCCAGCGTCAAGACTCACGAATCAGGCAAAACGAAAGACAGCGGAGCTCTACATGAAAATTCTGGTCACCGGCGCAAG
CGGCTTCATTGGCGGACGCT

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MFDYKLLSALAAVVEQAGFERAAQVLGLSQSAISQRIKLLEARVGQPVLVRGTPPSPTEIGRRLLNHVQQVRLLERDLQT
LVPALDEEGLPERLRIALNADSLATWWAEAVGDFCAEQHLLLDLIVEDQTVGLKRMRAGEVAGCLCASERPVAGARSVLL
GAMRYRALASPAFIKRHFPDGVRAEQLPRTPALVFGPDDFLQHRYLASLGVDGGFEHHLCPSSEGFIRLTEAGLGWGLVP
ELQVREQLERGVLRELLPDKPIDVPLYWHHWRNGGQLLGLLTEQLVRSSAQWLVPLD

Sequences:

>Translated_297_residues
MFDYKLLSALAAVVEQAGFERAAQVLGLSQSAISQRIKLLEARVGQPVLVRGTPPSPTEIGRRLLNHVQQVRLLERDLQT
LVPALDEEGLPERLRIALNADSLATWWAEAVGDFCAEQHLLLDLIVEDQTVGLKRMRAGEVAGCLCASERPVAGARSVLL
GAMRYRALASPAFIKRHFPDGVRAEQLPRTPALVFGPDDFLQHRYLASLGVDGGFEHHLCPSSEGFIRLTEAGLGWGLVP
ELQVREQLERGVLRELLPDKPIDVPLYWHHWRNGGQLLGLLTEQLVRSSAQWLVPLD
>Mature_297_residues
MFDYKLLSALAAVVEQAGFERAAQVLGLSQSAISQRIKLLEARVGQPVLVRGTPPSPTEIGRRLLNHVQQVRLLERDLQT
LVPALDEEGLPERLRIALNADSLATWWAEAVGDFCAEQHLLLDLIVEDQTVGLKRMRAGEVAGCLCASERPVAGARSVLL
GAMRYRALASPAFIKRHFPDGVRAEQLPRTPALVFGPDDFLQHRYLASLGVDGGFEHHLCPSSEGFIRLTEAGLGWGLVP
ELQVREQLERGVLRELLPDKPIDVPLYWHHWRNGGQLLGLLTEQLVRSSAQWLVPLD

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=291, Percent_Identity=37.8006872852234, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32896; Mature: 32896

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYKLLSALAAVVEQAGFERAAQVLGLSQSAISQRIKLLEARVGQPVLVRGTPPSPTEI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
GRRLLNHVQQVRLLERDLQTLVPALDEEGLPERLRIALNADSLATWWAEAVGDFCAEQHL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECCCHHHHHHHHHHHHHHHHHHH
LLDLIVEDQTVGLKRMRAGEVAGCLCASERPVAGARSVLLGAMRYRALASPAFIKRHFPD
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCC
GVRAEQLPRTPALVFGPDDFLQHRYLASLGVDGGFEHHLCPSSEGFIRLTEAGLGWGLVP
CCCHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
ELQVREQLERGVLRELLPDKPIDVPLYWHHWRNGGQLLGLLTEQLVRSSAQWLVPLD
CHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MFDYKLLSALAAVVEQAGFERAAQVLGLSQSAISQRIKLLEARVGQPVLVRGTPPSPTEI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
GRRLLNHVQQVRLLERDLQTLVPALDEEGLPERLRIALNADSLATWWAEAVGDFCAEQHL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEECCCHHHHHHHHHHHHHHHHHHH
LLDLIVEDQTVGLKRMRAGEVAGCLCASERPVAGARSVLLGAMRYRALASPAFIKRHFPD
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCC
GVRAEQLPRTPALVFGPDDFLQHRYLASLGVDGGFEHHLCPSSEGFIRLTEAGLGWGLVP
CCCHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
ELQVREQLERGVLRELLPDKPIDVPLYWHHWRNGGQLLGLLTEQLVRSSAQWLVPLD
CHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA