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The map label for this gene is gcvA [H]

Identifier: 77460693

GI number: 77460693

Start: 5044271

End: 5045149

Strand: Direct

Name: gcvA [H]

Synonym: Pfl01_4472

Alternate gene names: 77460693

Gene position: 5044271-5045149 (Clockwise)

Preceding gene: 77460686

Following gene: 77460696

Centisome position: 78.35

GC content: 63.03

Gene sequence:

>879_bases
ATGAAACGACTGCCTCCCCTGCCCGCTCTGCATACGTTTCTGATCACCGCGCAGTGCTGCAACTTCACCCGGGCCGCCGA
GCAGTTGCACATCACCCAGGGCGCGGTGAGCCGGCAGATCGCCGGGCTTGAGGAACATCTGGGTTATGAGCTGTTCATTC
GCCTGGCCCGGGGTTTGGAGCTGACGGCCGAAGGTCGCGAATGGCTGCCACGGGTGCAGCAGGTGTTCAGCCTGATCGGC
GACTCGGTGGAGCAGATCGGCGCCAAACGCGAAACCCTGCAACTCAAGGCGCCGAGCTGCGTGATGCGCTGGTTGTTGCC
ACGCCTGCTGCAATGGCAGCGCGAGCGCCCGGACGTGCCGGTGAAACTCACCGCGTCGTTGCAACACGGGGTGGACTTTC
ATCGCGAGCAGTTCGACGCGGCGGTGATCTACGGCAGCGCGCCCCACGATTCGCCTGCGACCCTGCATCTGTTCGATGAA
CAACTGACGCCGGTCTGTTCGCCGACACTGCTCAACGGAGGGCCGGCCCTGCATACACCGGCAGATCTGCAACAGCATCT
GCTGCTGCACCCGACTCATACGGATGACGACTGGACGCTCTGGCTCAAAGCCGCCGAACTGCACTTGAGCAATATCGCCA
GCGGTCAGCATTTCGAGACGCTGGATCAGGCGATGTCGATGGCGTCCCACGGGACGGGGGTGGCGATCGGCGACTGGTCA
CTGATCGGCGACGACTTGAACGCCGGGCGACTGGTGATGCCGTTCGATCTGAAGGTGAAGACGGGATTGGGCTATTACAT
CGTGGCGCCGGCGTCGGAGCCGTCGGCGAAATTGCAGGAATTGATGGGGTGGTTGGTGGAGCAGGCTCATACGCGGTGA

Upstream 100 bases:

>100_bases
AGGAACCGGTGATTGGCCTCATTGAAACGGGCGACCCGTAGCCGCAACAATCGATAAAAGCTCATACTAGCCATGTCCTC
ACGTCATACCTTGGATCCAC

Downstream 100 bases:

>100_bases
CGCCTTGCCCTCACCCTAACCCTCTCCCAGGGGGAGAGGGAACTGACCGAAGTGTCTTACGTAATACATCGACCTGAAAA
ATCTGGGCGATTATGGATTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKRLPPLPALHTFLITAQCCNFTRAAEQLHITQGAVSRQIAGLEEHLGYELFIRLARGLELTAEGREWLPRVQQVFSLIG
DSVEQIGAKRETLQLKAPSCVMRWLLPRLLQWQRERPDVPVKLTASLQHGVDFHREQFDAAVIYGSAPHDSPATLHLFDE
QLTPVCSPTLLNGGPALHTPADLQQHLLLHPTHTDDDWTLWLKAAELHLSNIASGQHFETLDQAMSMASHGTGVAIGDWS
LIGDDLNAGRLVMPFDLKVKTGLGYYIVAPASEPSAKLQELMGWLVEQAHTR

Sequences:

>Translated_292_residues
MKRLPPLPALHTFLITAQCCNFTRAAEQLHITQGAVSRQIAGLEEHLGYELFIRLARGLELTAEGREWLPRVQQVFSLIG
DSVEQIGAKRETLQLKAPSCVMRWLLPRLLQWQRERPDVPVKLTASLQHGVDFHREQFDAAVIYGSAPHDSPATLHLFDE
QLTPVCSPTLLNGGPALHTPADLQQHLLLHPTHTDDDWTLWLKAAELHLSNIASGQHFETLDQAMSMASHGTGVAIGDWS
LIGDDLNAGRLVMPFDLKVKTGLGYYIVAPASEPSAKLQELMGWLVEQAHTR
>Mature_292_residues
MKRLPPLPALHTFLITAQCCNFTRAAEQLHITQGAVSRQIAGLEEHLGYELFIRLARGLELTAEGREWLPRVQQVFSLIG
DSVEQIGAKRETLQLKAPSCVMRWLLPRLLQWQRERPDVPVKLTASLQHGVDFHREQFDAAVIYGSAPHDSPATLHLFDE
QLTPVCSPTLLNGGPALHTPADLQQHLLLHPTHTDDDWTLWLKAAELHLSNIASGQHFETLDQAMSMASHGTGVAIGDWS
LIGDDLNAGRLVMPFDLKVKTGLGYYIVAPASEPSAKLQELMGWLVEQAHTR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=34.5890410958904, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=31.1864406779661, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1786448, Length=270, Percent_Identity=31.4814814814815, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786401, Length=251, Percent_Identity=27.8884462151394, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693193, Length=189, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790262, Length=208, Percent_Identity=26.4423076923077, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32453; Mature: 32453

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLPPLPALHTFLITAQCCNFTRAAEQLHITQGAVSRQIAGLEEHLGYELFIRLARGLE
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
LTAEGREWLPRVQQVFSLIGDSVEQIGAKRETLQLKAPSCVMRWLLPRLLQWQRERPDVP
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCC
VKLTASLQHGVDFHREQFDAAVIYGSAPHDSPATLHLFDEQLTPVCSPTLLNGGPALHTP
EEEECCHHHCHHHHHHHCCEEEEECCCCCCCCCEEEEEHHHCCCCCCCCEECCCCCCCCH
ADLQQHLLLHPTHTDDDWTLWLKAAELHLSNIASGQHFETLDQAMSMASHGTGVAIGDWS
HHHHHCEEECCCCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCE
LIGDDLNAGRLVMPFDLKVKTGLGYYIVAPASEPSAKLQELMGWLVEQAHTR
EECCCCCCCEEEEEECEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRLPPLPALHTFLITAQCCNFTRAAEQLHITQGAVSRQIAGLEEHLGYELFIRLARGLE
CCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
LTAEGREWLPRVQQVFSLIGDSVEQIGAKRETLQLKAPSCVMRWLLPRLLQWQRERPDVP
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCC
VKLTASLQHGVDFHREQFDAAVIYGSAPHDSPATLHLFDEQLTPVCSPTLLNGGPALHTP
EEEECCHHHCHHHHHHHCCEEEEECCCCCCCCCEEEEEHHHCCCCCCCCEECCCCCCCCH
ADLQQHLLLHPTHTDDDWTLWLKAAELHLSNIASGQHFETLDQAMSMASHGTGVAIGDWS
HHHHHCEEECCCCCCCCEEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCE
LIGDDLNAGRLVMPFDLKVKTGLGYYIVAPASEPSAKLQELMGWLVEQAHTR
EECCCCCCCEEEEEECEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]