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The map label for this gene is potB [H]

Identifier: 77460689

GI number: 77460689

Start: 5038746

End: 5039654

Strand: Reverse

Name: potB [H]

Synonym: Pfl01_4468

Alternate gene names: 77460689

Gene position: 5039654-5038746 (Counterclockwise)

Preceding gene: 77460690

Following gene: 77460688

Centisome position: 78.27

GC content: 64.8

Gene sequence:

>909_bases
ATGGAACATCAATCCCTGACCCAACCGGTCGGCACGGGTGACGTGCGCCCGGCGCGCGGTGTTTCGCCGACCGCGCGGGC
GTGGTTTTTCCTCACGCCGTCGATGCTGTTTCTCGGCGTGCTGATTGCCGCCAGCCTGCTGGTGCTGCGCATGAGCGTCG
GCACCAAGGGGGCGGAGTGGTCCGGTTTCAGCCTGGCCAGTTACGCGCAGTTGCTCGAACCCTACTACCTCAAATCGTTG
CTGCTGACCTTGCGTCTGGCGCTGATCAGCGCGGTGGTCGCGGTGGTGCTGGCGATCCCGGTGGCGTACACCATGTCGCG
CCTGACCTCGCCATTCGTGCGGCGGATTTTTCTGGCGGCGGTGCTGTTGCCGCTGCTGGTCAACCTGTTGCTGCAAAGCT
ACGGCTGGCTGGTGATCCTCGGCCCCGGCGGCATGCTCAACCAGACCCTGATGGGCCTGGGTCTGATCAAGCGGCCGATC
ATGTTGCTGTACAACCAGAATGGCGTGTTGATGGGCCTGGTGCAGACCGCGTTCCCGCTGGCCGTGCTGCCGATTGCCAG
CGCCATGCGCGGTGTCGCCCGCAGCTACGAAGAAGCTGCCGCCACCCTCGGTGCCAGTCGTTTTCAGGTGTTCCGCCAGG
TGGTGTTGCCAATGAGTTTGCCGGGAATCATCACCGGCGCGACCCTGGTGTTCGCCTACAACGCCAGCAGCTTCGTGGTG
CCGCTGTTGCTCGGTGGTCGGCGCGTGCCGATGCTGGCGGTGATGGTGCATGACCAGATCGCCCCGCTGATGAACTGGCC
TGCCGCGTCCGCCGCCGGGGTGGTGCTGATCGTCACTACGCTCGCCATCATGACCTTGTCCGAATACATCACCGGCCGCC
GTCGCCGTCTGCTGGAGGCTTCGCAATGA

Upstream 100 bases:

>100_bases
GTCGCTACACGGAAATCTTTTCTTCCTGACGCCGACCTTCCGGGGCACCGCATGATCGGGGTGCCCGGGAGATTCGTTTT
CCATTGACGAGATCATTGCT

Downstream 100 bases:

>100_bases
GTGCCCTGATCAAGAAGCGTCAGTCGCTGTTGCCGGGCGACACCGGCAAGTTCGCCGGCATCCTGTCCGGGATCATTTTG
TTCCTGGCCGTGTTGCCGAT

Product: binding-protein dependent transport system inner membrane protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MEHQSLTQPVGTGDVRPARGVSPTARAWFFLTPSMLFLGVLIAASLLVLRMSVGTKGAEWSGFSLASYAQLLEPYYLKSL
LLTLRLALISAVVAVVLAIPVAYTMSRLTSPFVRRIFLAAVLLPLLVNLLLQSYGWLVILGPGGMLNQTLMGLGLIKRPI
MLLYNQNGVLMGLVQTAFPLAVLPIASAMRGVARSYEEAAATLGASRFQVFRQVVLPMSLPGIITGATLVFAYNASSFVV
PLLLGGRRVPMLAVMVHDQIAPLMNWPAASAAGVVLIVTTLAIMTLSEYITGRRRRLLEASQ

Sequences:

>Translated_302_residues
MEHQSLTQPVGTGDVRPARGVSPTARAWFFLTPSMLFLGVLIAASLLVLRMSVGTKGAEWSGFSLASYAQLLEPYYLKSL
LLTLRLALISAVVAVVLAIPVAYTMSRLTSPFVRRIFLAAVLLPLLVNLLLQSYGWLVILGPGGMLNQTLMGLGLIKRPI
MLLYNQNGVLMGLVQTAFPLAVLPIASAMRGVARSYEEAAATLGASRFQVFRQVVLPMSLPGIITGATLVFAYNASSFVV
PLLLGGRRVPMLAVMVHDQIAPLMNWPAASAAGVVLIVTTLAIMTLSEYITGRRRRLLEASQ
>Mature_302_residues
MEHQSLTQPVGTGDVRPARGVSPTARAWFFLTPSMLFLGVLIAASLLVLRMSVGTKGAEWSGFSLASYAQLLEPYYLKSL
LLTLRLALISAVVAVVLAIPVAYTMSRLTSPFVRRIFLAAVLLPLLVNLLLQSYGWLVILGPGGMLNQTLMGLGLIKRPI
MLLYNQNGVLMGLVQTAFPLAVLPIASAMRGVARSYEEAAATLGASRFQVFRQVVLPMSLPGIITGATLVFAYNASSFVV
PLLLGGRRVPMLAVMVHDQIAPLMNWPAASAAGVVLIVTTLAIMTLSEYITGRRRRLLEASQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=239, Percent_Identity=32.2175732217573, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787080, Length=231, Percent_Identity=32.4675324675325, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787713, Length=228, Percent_Identity=29.3859649122807, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786980, Length=168, Percent_Identity=30.3571428571429, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32493; Mature: 32493

Theoretical pI: Translated: 11.33; Mature: 11.33

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHQSLTQPVGTGDVRPARGVSPTARAWFFLTPSMLFLGVLIAASLLVLRMSVGTKGAEW
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGFSLASYAQLLEPYYLKSLLLTLRLALISAVVAVVLAIPVAYTMSRLTSPFVRRIFLAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLPLLVNLLLQSYGWLVILGPGGMLNQTLMGLGLIKRPIMLLYNQNGVLMGLVQTAFPL
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
AVLPIASAMRGVARSYEEAAATLGASRFQVFRQVVLPMSLPGIITGATLVFAYNASSFVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEECCCHHHH
PLLLGGRRVPMLAVMVHDQIAPLMNWPAASAAGVVLIVTTLAIMTLSEYITGRRRRLLEA
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SQ
CC
>Mature Secondary Structure
MEHQSLTQPVGTGDVRPARGVSPTARAWFFLTPSMLFLGVLIAASLLVLRMSVGTKGAEW
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SGFSLASYAQLLEPYYLKSLLLTLRLALISAVVAVVLAIPVAYTMSRLTSPFVRRIFLAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLPLLVNLLLQSYGWLVILGPGGMLNQTLMGLGLIKRPIMLLYNQNGVLMGLVQTAFPL
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHH
AVLPIASAMRGVARSYEEAAATLGASRFQVFRQVVLPMSLPGIITGATLVFAYNASSFVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEECCCHHHH
PLLLGGRRVPMLAVMVHDQIAPLMNWPAASAAGVVLIVTTLAIMTLSEYITGRRRRLLEA
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]