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The map label for this gene is purU [H]

Identifier: 77460657

GI number: 77460657

Start: 4995915

End: 4996763

Strand: Reverse

Name: purU [H]

Synonym: Pfl01_4436

Alternate gene names: 77460657

Gene position: 4996763-4995915 (Counterclockwise)

Preceding gene: 77460659

Following gene: 77460656

Centisome position: 77.61

GC content: 60.31

Gene sequence:

>849_bases
ATGCGCACTTTTCGGCTGGTGATTTCTTGCCCGGACCGCGTTGGCATCGTTGCCAAAGTCAGTAACTTTCTGGCGGCCCA
CAATGGCTGGATCACCGAAGCGAGCCACCACTCGGATAACCAGGTCGGCTGGTTCTTCATGCGTCACGAAATCCGTGCCG
ATTCCCTGCCTTTTGGTATCGAGGTGTTGCGCGAGAAGTTTGCACCGATCGCCGAAGAGTTCTCGATGGACTGGCGCATC
ACCGACACCGAGCAGAAAAAACGCGTGGTGCTGATGGCCAGCCGCGAATCCCACTGCCTGGCCGACCTGCTGCACCGCTG
GCACAGCGACGAGCTCGATTGCGAGATCTCCTGCGTGATTTCCAACCACGACGACCTGCGCAGCATGGTCGAGTGGCACG
GCATTCCTTATTACCACGTGCCGGTCAATCCGCAGGACAAGCAACCGGCCTTCGATGAAGTGTCGCGCCTGGTCAAGCAG
CACGACGCCGAAGTCGTGGTGCTGGCGCGCTACATGCAGATCCTGCCGCCGGACATGTGCCGCGAATACGCGCACAAGGT
CATCAACATTCACCACAGCTTCCTGCCGTCGTTCGTCGGTGCCAAGCCGTACCACCAGGCTTCGATGCGCGGCGTGAAGC
TGATCGGTGCGACTTGCCATTACGTGACCGAAGAGCTGGACGCCGGCCCGATCATCGAGCAGGACGTGGTACGTGTCAGC
CACAGCGACAGCATCGAAGACATGGTGCGTTTCGGCCGTGACGTCGAGAAGATGGTGCTGGCCCGTGGCCTGCGCTATCA
CCTGGAAGACCGCGTGCTGGTGCACGGCAACAAGACCGTGGTGTTCTGA

Upstream 100 bases:

>100_bases
GCGACGGGGTGCCGTAAGCCTCCGAGGGTCATCAATACTGCAGTTTTCCCGCGCAGTCGCTAGAATGGCCGCCTTTGCGA
AGTTCTGGAGTTTCCCCCTC

Downstream 100 bases:

>100_bases
TCAGACCTTTCGGTTGAAAAACAAAGGGCCTGGGCGTTCAATCGCCTCAGGCCCTTTTTTTATGACTTGTTGCAGCCCTG
TAAGGAAGCGTTCATGAGCG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRTFRLVISCPDRVGIVAKVSNFLAAHNGWITEASHHSDNQVGWFFMRHEIRADSLPFGIEVLREKFAPIAEEFSMDWRI
TDTEQKKRVVLMASRESHCLADLLHRWHSDELDCEISCVISNHDDLRSMVEWHGIPYYHVPVNPQDKQPAFDEVSRLVKQ
HDAEVVVLARYMQILPPDMCREYAHKVINIHHSFLPSFVGAKPYHQASMRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HSDSIEDMVRFGRDVEKMVLARGLRYHLEDRVLVHGNKTVVF

Sequences:

>Translated_282_residues
MRTFRLVISCPDRVGIVAKVSNFLAAHNGWITEASHHSDNQVGWFFMRHEIRADSLPFGIEVLREKFAPIAEEFSMDWRI
TDTEQKKRVVLMASRESHCLADLLHRWHSDELDCEISCVISNHDDLRSMVEWHGIPYYHVPVNPQDKQPAFDEVSRLVKQ
HDAEVVVLARYMQILPPDMCREYAHKVINIHHSFLPSFVGAKPYHQASMRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HSDSIEDMVRFGRDVEKMVLARGLRYHLEDRVLVHGNKTVVF
>Mature_282_residues
MRTFRLVISCPDRVGIVAKVSNFLAAHNGWITEASHHSDNQVGWFFMRHEIRADSLPFGIEVLREKFAPIAEEFSMDWRI
TDTEQKKRVVLMASRESHCLADLLHRWHSDELDCEISCVISNHDDLRSMVEWHGIPYYHVPVNPQDKQPAFDEVSRLVKQ
HDAEVVVLARYMQILPPDMCREYAHKVINIHHSFLPSFVGAKPYHQASMRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HSDSIEDMVRFGRDVEKMVLARGLRYHLEDRVLVHGNKTVVF

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=168, Percent_Identity=31.547619047619, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI209869995, Length=168, Percent_Identity=31.547619047619, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI209869993, Length=168, Percent_Identity=31.547619047619, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787483, Length=273, Percent_Identity=42.1245421245421, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788846, Length=193, Percent_Identity=27.979274611399, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=170, Percent_Identity=31.7647058823529, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=26.7379679144385, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32544; Mature: 32544

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTFRLVISCPDRVGIVAKVSNFLAAHNGWITEASHHSDNQVGWFFMRHEIRADSLPFGI
CCEEEEEEECCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEEEHHCCCCCCHHH
EVLREKFAPIAEEFSMDWRITDTEQKKRVVLMASRESHCLADLLHRWHSDELDCEISCVI
HHHHHHHCCHHHHHCCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEE
SNHDDLRSMVEWHGIPYYHVPVNPQDKQPAFDEVSRLVKQHDAEVVVLARYMQILPPDMC
CCCHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHH
REYAHKVINIHHSFLPSFVGAKPYHQASMRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHC
HSDSIEDMVRFGRDVEKMVLARGLRYHLEDRVLVHGNKTVVF
CCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCEEEEC
>Mature Secondary Structure
MRTFRLVISCPDRVGIVAKVSNFLAAHNGWITEASHHSDNQVGWFFMRHEIRADSLPFGI
CCEEEEEEECCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEEEHHCCCCCCHHH
EVLREKFAPIAEEFSMDWRITDTEQKKRVVLMASRESHCLADLLHRWHSDELDCEISCVI
HHHHHHHCCHHHHHCCCEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEE
SNHDDLRSMVEWHGIPYYHVPVNPQDKQPAFDEVSRLVKQHDAEVVVLARYMQILPPDMC
CCCHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHH
REYAHKVINIHHSFLPSFVGAKPYHQASMRGVKLIGATCHYVTEELDAGPIIEQDVVRVS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHC
HSDSIEDMVRFGRDVEKMVLARGLRYHLEDRVLVHGNKTVVF
CCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]