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The map label for this gene is tapB [H]

Identifier: 77460616

GI number: 77460616

Start: 4957352

End: 4957564

Strand: Direct

Name: tapB [H]

Synonym: Pfl01_4395

Alternate gene names: 77460616

Gene position: 4957352-4957564 (Clockwise)

Preceding gene: 77460615

Following gene: 77460617

Centisome position: 77.0

GC content: 52.11

Gene sequence:

>213_bases
ATGTCCCAACGTCAGAGCGGTACCGTCAAGTGGTTTAACGACGAGAAAGGTTTTGGTTTTATCACTCCAGAAAGCGGTCC
GGATCTGTTCGTGCATTTCCGCGCCATTCAGGGCAACGGCTTCAAGAGCCTGAAAGAAGGCCAGAAAGTGACCTTCGTTG
CAGTGCAAGGCCAGAAAGGCATGCAAGCTGACGAAGTCATCGCAGAAGCCTGA

Upstream 100 bases:

>100_bases
TACATCCCGTTCGACGTCTTCTTACTCTCCTGCAACCAGCCCCAGTACTCTTTCATGAGGAAGAGACTGTCATCAATTTT
TGCGTCAAAGGAAATAAGAA

Downstream 100 bases:

>100_bases
TTTTCTGTAACGAAAAAGCCCCTGATATTGATATCAGGGGCTTTTTTGTGGGCGCAAATCCGTAAAATGGCGCTTCACTT
TCCGTCCAGAGGCTGCCATG

Product: cold-shock DNA-binding protein

Products: NA

Alternate protein names: E8.0 [H]

Number of amino acids: Translated: 70; Mature: 69

Protein sequence:

>70_residues
MSQRQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKGMQADEVIAEA

Sequences:

>Translated_70_residues
MSQRQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKGMQADEVIAEA
>Mature_69_residues
SQRQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKGMQADEVIAEA

Specific function: Affects cell viability at low temperatures [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=69, Percent_Identity=57.9710144927536, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1786841, Length=63, Percent_Identity=65.0793650793651, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI2367114, Length=69, Percent_Identity=56.5217391304348, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1787834, Length=69, Percent_Identity=57.9710144927536, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1788126, Length=63, Percent_Identity=65.0793650793651, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=55.8823529411765, Blast_Score=76, Evalue=4e-16,
Organism=Escherichia coli, GI1787107, Length=64, Percent_Identity=51.5625, Blast_Score=70, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7707; Mature: 7576

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKG
CCCCCCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCC
MQADEVIAEA
CCCHHHEECC
>Mature Secondary Structure 
SQRQSGTVKWFNDEKGFGFITPESGPDLFVHFRAIQGNGFKSLKEGQKVTFVAVQGQKG
CCCCCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCCC
MQADEVIAEA
CCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9393697 [H]