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The map label for this gene is nrdB [H]

Identifier: 77460516

GI number: 77460516

Start: 4852604

End: 4853854

Strand: Direct

Name: nrdB [H]

Synonym: Pfl01_4295

Alternate gene names: 77460516

Gene position: 4852604-4853854 (Clockwise)

Preceding gene: 77460515

Following gene: 77460517

Centisome position: 75.37

GC content: 58.99

Gene sequence:

>1251_bases
ATGCTGAGCTGGGACGAATTCGACAAAGAAGACAGTGAAGTAGCAGCAGTGAAAGGCGCCAACGCCGGCCACGCTAGCGA
AGCCAACATGGACCGCCTCGACAGCGCCGGCGGTGCCGCCGCCCTCGAAGCCCGCGCCGTGACCGCCGAAGACTCGGCCG
CCGTGGCCCGCGCCAAGGCTGCACTGGATTCCCTCGACGTCGCCGAAGGCCTCGCCGAACTCGAAGGCTCCGCCGCCCGT
GTCGCCGTTGACGAAAAGCGCATGATCAACTGCCGCGCCGACCTCAACCAGCTCGTGCCATTCAAGTACGACTGGGCCTG
GCAGAAATACCTGGACGGCTGCGCAAACCACTGGATGCCGCAAGAAGTCAACATGACCGCCGACATCGCCCTCTGGAAAA
ACCCGGAAGGCCTGACCGACGACGAGCGCCGCATCGTGATGCGCAACCTCGGCTTCTTCTCCACCGCCGACTCCCTGGTT
GCCAACAACCTGGCCCTGGCCGTGTACCGCCTGATCACCAACCCGGAATGCCGCCAGTACATCCTGCGCCAGGCCTTCGA
AGAGGCGATCCACACCCACGCCTACCAGTACTGCATCGAATCGCTGGGCATGGATGAAGGCGAGATCTTCAACATGTACC
ACGAGATCCCGTCGGTCGCGAAAAAAGCCGCCTGGGGCCTGAAATACACCCGCGCCATCTCCGACCCGGAATTCAACACC
GGCACCGTAGAGACCGACAAAGAACTGCTGCGCAACCTGATCGCCTACTACTGCGTTCTGGAAGGCATCTTCTTCTACTG
CGGCTTCACCCAGATCCTCTCCATGGGCCGCCGCAACAAAATGACCGGCGTCGCCGAGCAGTTCCAGTACATCCTGCGCG
ACGAATCCATGCACCTGAACTTCGGCATCGACGTGATCAACCAGATCAAAATCGAAAACCCACACCTGTGGGATGCTGAA
ATGAAGGAAGAAGCTTCGCAGATGATCCTGCAAGGCACTCAGCTGGAAATCGAATACGCGCGTGACACCATGCCTCGCGG
CGTACTGGGCATGAACGCGGCGATGATGGAGGACTACCTGAAGTTCATCGCTAACCGTCGTCTGTCGCAGATCGGTTTGA
AGGAAGAGTACCCAGGGACGACTAACCCGTTCCCTTGGATGAGCGAGATTATGGACTTGAAGAAAGAGAAGAATTTCTTT
GAGACTCGTGTGATCGAATACCAAACTGGTGGTGCTCTGAGCTGGGACTAA

Upstream 100 bases:

>100_bases
CTGCAAAAAACGACATCCAACGCGGCCTGTACGGCCGTGTGAAAAACCAACCAATCAGTTTTCACACGGGCACAGGCCCT
CACGCAGGAGACGACACGTC

Downstream 100 bases:

>100_bases
TCGTCTCGCTCGCCACGATTTGACGATTGCTGCTTGAGTCGTCGAATTGAGAAAGCAAAAAGCCCCGATTTGATCGGGGC
TTTTTTATGGGCGGCTGGTA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MLSWDEFDKEDSEVAAVKGANAGHASEANMDRLDSAGGAAALEARAVTAEDSAAVARAKAALDSLDVAEGLAELEGSAAR
VAVDEKRMINCRADLNQLVPFKYDWAWQKYLDGCANHWMPQEVNMTADIALWKNPEGLTDDERRIVMRNLGFFSTADSLV
ANNLALAVYRLITNPECRQYILRQAFEEAIHTHAYQYCIESLGMDEGEIFNMYHEIPSVAKKAAWGLKYTRAISDPEFNT
GTVETDKELLRNLIAYYCVLEGIFFYCGFTQILSMGRRNKMTGVAEQFQYILRDESMHLNFGIDVINQIKIENPHLWDAE
MKEEASQMILQGTQLEIEYARDTMPRGVLGMNAAMMEDYLKFIANRRLSQIGLKEEYPGTTNPFPWMSEIMDLKKEKNFF
ETRVIEYQTGGALSWD

Sequences:

>Translated_416_residues
MLSWDEFDKEDSEVAAVKGANAGHASEANMDRLDSAGGAAALEARAVTAEDSAAVARAKAALDSLDVAEGLAELEGSAAR
VAVDEKRMINCRADLNQLVPFKYDWAWQKYLDGCANHWMPQEVNMTADIALWKNPEGLTDDERRIVMRNLGFFSTADSLV
ANNLALAVYRLITNPECRQYILRQAFEEAIHTHAYQYCIESLGMDEGEIFNMYHEIPSVAKKAAWGLKYTRAISDPEFNT
GTVETDKELLRNLIAYYCVLEGIFFYCGFTQILSMGRRNKMTGVAEQFQYILRDESMHLNFGIDVINQIKIENPHLWDAE
MKEEASQMILQGTQLEIEYARDTMPRGVLGMNAAMMEDYLKFIANRRLSQIGLKEEYPGTTNPFPWMSEIMDLKKEKNFF
ETRVIEYQTGGALSWD
>Mature_416_residues
MLSWDEFDKEDSEVAAVKGANAGHASEANMDRLDSAGGAAALEARAVTAEDSAAVARAKAALDSLDVAEGLAELEGSAAR
VAVDEKRMINCRADLNQLVPFKYDWAWQKYLDGCANHWMPQEVNMTADIALWKNPEGLTDDERRIVMRNLGFFSTADSLV
ANNLALAVYRLITNPECRQYILRQAFEEAIHTHAYQYCIESLGMDEGEIFNMYHEIPSVAKKAAWGLKYTRAISDPEFNT
GTVETDKELLRNLIAYYCVLEGIFFYCGFTQILSMGRRNKMTGVAEQFQYILRDESMHLNFGIDVINQIKIENPHLWDAE
MKEEASQMILQGTQLEIEYARDTMPRGVLGMNAAMMEDYLKFIANRRLSQIGLKEEYPGTTNPFPWMSEIMDLKKEKNFF
ETRVIEYQTGGALSWD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI260064013, Length=362, Percent_Identity=27.9005524861878, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI4557845, Length=362, Percent_Identity=27.9005524861878, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI289177074, Length=320, Percent_Identity=27.8125, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI42544136, Length=320, Percent_Identity=27.8125, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI289177076, Length=296, Percent_Identity=28.0405405405405, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI1788567, Length=293, Percent_Identity=24.9146757679181, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17551968, Length=321, Percent_Identity=29.595015576324, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI193206379, Length=297, Percent_Identity=24.5791245791246, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17567153, Length=224, Percent_Identity=25.8928571428571, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322434, Length=334, Percent_Identity=29.6407185628743, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321619, Length=320, Percent_Identity=23.125, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI18079279, Length=320, Percent_Identity=30.625, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 47101; Mature: 47101

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSWDEFDKEDSEVAAVKGANAGHASEANMDRLDSAGGAAALEARAVTAEDSAAVARAKA
CCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHEECCCHHHHHHHHHH
ALDSLDVAEGLAELEGSAARVAVDEKRMINCRADLNQLVPFKYDWAWQKYLDGCANHWMP
HHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
QEVNMTADIALWKNPEGLTDDERRIVMRNLGFFSTADSLVANNLALAVYRLITNPECRQY
CCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ILRQAFEEAIHTHAYQYCIESLGMDEGEIFNMYHEIPSVAKKAAWGLKYTRAISDPEFNT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCC
GTVETDKELLRNLIAYYCVLEGIFFYCGFTQILSMGRRNKMTGVAEQFQYILRDESMHLN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEE
FGIDVINQIKIENPHLWDAEMKEEASQMILQGTQLEIEYARDTMPRGVLGMNAAMMEDYL
ECHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEHHCCCCCCHHHCHHHHHHHHH
KFIANRRLSQIGLKEEYPGTTNPFPWMSEIMDLKKEKNFFETRVIEYQTGGALSWD
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCC
>Mature Secondary Structure
MLSWDEFDKEDSEVAAVKGANAGHASEANMDRLDSAGGAAALEARAVTAEDSAAVARAKA
CCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHEECCCHHHHHHHHHH
ALDSLDVAEGLAELEGSAARVAVDEKRMINCRADLNQLVPFKYDWAWQKYLDGCANHWMP
HHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
QEVNMTADIALWKNPEGLTDDERRIVMRNLGFFSTADSLVANNLALAVYRLITNPECRQY
CCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ILRQAFEEAIHTHAYQYCIESLGMDEGEIFNMYHEIPSVAKKAAWGLKYTRAISDPEFNT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCC
GTVETDKELLRNLIAYYCVLEGIFFYCGFTQILSMGRRNKMTGVAEQFQYILRDESMHLN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEE
FGIDVINQIKIENPHLWDAEMKEEASQMILQGTQLEIEYARDTMPRGVLGMNAAMMEDYL
ECHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEHHCCCCCCHHHCHHHHHHHHH
KFIANRRLSQIGLKEEYPGTTNPFPWMSEIMDLKKEKNFFETRVIEYQTGGALSWD
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]