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The map label for this gene is usf [H]

Identifier: 77460463

GI number: 77460463

Start: 4784106

End: 4784897

Strand: Direct

Name: usf [H]

Synonym: Pfl01_4242

Alternate gene names: 77460463

Gene position: 4784106-4784897 (Clockwise)

Preceding gene: 77460458

Following gene: 77460464

Centisome position: 74.31

GC content: 62.37

Gene sequence:

>792_bases
ATGCGCCTGCTTCTAACCCTCGCCCTCCTTGTCTTCAGCGGCCTCAGCCAGGCTGCGATCAAGACCGAGGAAATCCCCTA
TCAGAGTGCTGACGGCACCCAGCTGATCGGTTACTACGCCTACGACGACGCGATCAAGGGCCCGCGCCCCGGCGTGGTGG
TGGTGCATGAATGGTGGGGCCTGAACGACTACGCCAAGCGTCGCGCCCGGGATCTCGCCGGGCTCGGCTACAGCGCGCTG
GCCATCGACATGTACGGTGAAGGCAAGAACACCGAGCACCCGAAAGACGCGATGGCCTTCATGCAGGCGGCCACGCAGAA
CGCCGATGCAGCGAGCAAGCGCTTTCAGGCCGGGCTGGATCTGTTGAAGAAACAGCCGCAGACCGACAAGGATAAAATCG
CCGCCATCGGTTACTGCTTCGGCGGTGGCGTAGTGCTGAATGCCGCGCGTCAGGGTGTGCCGCTGGCCGGCGTGGTGAGC
TTTCACGGTGCGCTGGCCACCAAGACTCCGGCTGCGCCGGGCAGCGTGAAGGCGAAAATTCTCGTCGAACACGGCGCGTT
GGATAGCATGGTCACCCCGGACAACGTCGCGGCGTTCAAGTCGGAAATGGACAAGGCCGGCGCCGACTATCAATTCGTCA
GCCTCGACGGTGCCAAGCACGGCTTCAGCAATCCTGACGCGGATCGCCTGAGCCACGGCGATCACGGTGGCCCGGATATC
GGCTACAACAAGGCCGCCGATGAAAAATCCTGGTCGGACATGCAGGCGTTCTTCAAGAAAATCTTTGGCTGA

Upstream 100 bases:

>100_bases
TTACTGCCCCTCCCCTGTACCGCCCCTGAACGCTCTCTGAACCTGGCCTGAATGAAATGAACTAGGCTGTTTTGACCCTT
TATTGTCAGGAGACCCGATC

Downstream 100 bases:

>100_bases
TTCCAGTTAACCGATCGTTCCTGCGCTCTGCGTAGGAACGATCACTTCCTCTGCACTATCCAGCCTGAAGACAACCCGGC
AAAATGCCCGACATGACTGC

Product: dienelactone hydrolase

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRLLLTLALLVFSGLSQAAIKTEEIPYQSADGTQLIGYYAYDDAIKGPRPGVVVVHEWWGLNDYAKRRARDLAGLGYSAL
AIDMYGEGKNTEHPKDAMAFMQAATQNADAASKRFQAGLDLLKKQPQTDKDKIAAIGYCFGGGVVLNAARQGVPLAGVVS
FHGALATKTPAAPGSVKAKILVEHGALDSMVTPDNVAAFKSEMDKAGADYQFVSLDGAKHGFSNPDADRLSHGDHGGPDI
GYNKAADEKSWSDMQAFFKKIFG

Sequences:

>Translated_263_residues
MRLLLTLALLVFSGLSQAAIKTEEIPYQSADGTQLIGYYAYDDAIKGPRPGVVVVHEWWGLNDYAKRRARDLAGLGYSAL
AIDMYGEGKNTEHPKDAMAFMQAATQNADAASKRFQAGLDLLKKQPQTDKDKIAAIGYCFGGGVVLNAARQGVPLAGVVS
FHGALATKTPAAPGSVKAKILVEHGALDSMVTPDNVAAFKSEMDKAGADYQFVSLDGAKHGFSNPDADRLSHGDHGGPDI
GYNKAADEKSWSDMQAFFKKIFG
>Mature_263_residues
MRLLLTLALLVFSGLSQAAIKTEEIPYQSADGTQLIGYYAYDDAIKGPRPGVVVVHEWWGLNDYAKRRARDLAGLGYSAL
AIDMYGEGKNTEHPKDAMAFMQAATQNADAASKRFQAGLDLLKKQPQTDKDKIAAIGYCFGGGVVLNAARQGVPLAGVVS
FHGALATKTPAAPGSVKAKILVEHGALDSMVTPDNVAAFKSEMDKAGADYQFVSLDGAKHGFSNPDADRLSHGDHGGPDI
GYNKAADEKSWSDMQAFFKKIFG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI17532655, Length=247, Percent_Identity=34.412955465587, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17536419, Length=245, Percent_Identity=33.469387755102, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI193204305, Length=215, Percent_Identity=34.4186046511628, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17532653, Length=248, Percent_Identity=31.4516129032258, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17536415, Length=244, Percent_Identity=27.4590163934426, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17534829, Length=152, Percent_Identity=33.5526315789474, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 28070; Mature: 28070

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLLTLALLVFSGLSQAAIKTEEIPYQSADGTQLIGYYAYDDAIKGPRPGVVVVHEWWG
CHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCC
LNDYAKRRARDLAGLGYSALAIDMYGEGKNTEHPKDAMAFMQAATQNADAASKRFQAGLD
CCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLKKQPQTDKDKIAAIGYCFGGGVVLNAARQGVPLAGVVSFHGALATKTPAAPGSVKAKI
HHHCCCCCCHHHEEEEEEEECCCEEEEHHHCCCCEEHHHHHHHHHHCCCCCCCCCCEEEE
LVEHGALDSMVTPDNVAAFKSEMDKAGADYQFVSLDGAKHGFSNPDADRLSHGDHGGPDI
EEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCCC
GYNKAADEKSWSDMQAFFKKIFG
CCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRLLLTLALLVFSGLSQAAIKTEEIPYQSADGTQLIGYYAYDDAIKGPRPGVVVVHEWWG
CHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEECCCC
LNDYAKRRARDLAGLGYSALAIDMYGEGKNTEHPKDAMAFMQAATQNADAASKRFQAGLD
CCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLKKQPQTDKDKIAAIGYCFGGGVVLNAARQGVPLAGVVSFHGALATKTPAAPGSVKAKI
HHHCCCCCCHHHEEEEEEEECCCEEEEHHHCCCCEEHHHHHHHHHHCCCCCCCCCCEEEE
LVEHGALDSMVTPDNVAAFKSEMDKAGADYQFVSLDGAKHGFSNPDADRLSHGDHGGPDI
EEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCCC
GYNKAADEKSWSDMQAFFKKIFG
CCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]