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The map label for this gene is ddh [H]

Identifier: 77460444

GI number: 77460444

Start: 4768383

End: 4769372

Strand: Direct

Name: ddh [H]

Synonym: Pfl01_4223

Alternate gene names: 77460444

Gene position: 4768383-4769372 (Clockwise)

Preceding gene: 77460443

Following gene: 77460446

Centisome position: 74.06

GC content: 64.85

Gene sequence:

>990_bases
ATGCGCACGATCGTTTTCAGCAGCCAGTCCTACGACCGCGACAGTTTCCTCGCCGCCGACTGCCCGGCCGGGATCGAACT
GCATTTCCAGCCGGCCCGGCTCAGCCTCGACACGGCCGCGCTGGCCGAACATCACGAGGTGGTCTGCGCGTTCATCAATG
ATGACCTCAGCGCCCCGGTGCTCGAACGCCTGGCTGCGGGCGGGACACGCCTGATTGCCTTGCGTTCGGCCGGCTACAAC
CATGTGGACCTGGCCGTCGCCAAACGCTTGGAGCTGGCCGTAGTGCGGGTACCGGCCTACTCGCCCCATGCGGTGGCCGA
GCACGCGGTGGCGTTGATCCTCGCCCTCAACCGACGCCTGCACCGTGCCTACAACCGCACCCGCGAAGGCGATTTCAGCC
TGCACGGCCTCACCGGTTTCGATCTGGTGGGCAAAACGGTCGGCATCGTCGGCACCGGACAGATCGGCGCAACCTTCGCG
AAGATCATGCACGGCTTCGGCTGTGAATTGCTGGCCTACGACCCGTATCCAAATCCTGCCGTCGAAGCGTTGGGCGCACG
CTACCTGAGCCTGCCGGATCTGTTGGCGCAGTCGCGGATCATCAGCCTGCACTGCCCGCTCAACGAACAGAGCAGACACC
TGATCAACCGCGATTCGCTGGCGCACATGCAACCGGGGGCGATGCTGATCAACACCGGGCGCGGCGGTCTGGTGGACACG
CCGGCGCTGATCGACGCCCTGAAAGACGGTCAGCTCGGTTATCTGGGACTGGATGTGTATGAAGAGGAAGCGCAGCTGTT
TTTCGAGGACCGCTCCGACCTGCCGCTACAGGACGACGTGCTGGCGCGGCTGCTGACGTTCCCGAATGTGATCGTCACCG
CGCATCAGGCGTTCCTGACCCATGAGGCGCTGGGCGCGATTGCCGCGACCACCTTGCACAACATCGCGACCTGGGCGACG
GGCACGCCGCAGAACCAGGTCGAAGGCTGA

Upstream 100 bases:

>100_bases
CGAAGATCAGCGCCTGATCCAGCGCAATGCCGGTCAACGTCGACGCAGCTACAGTTGTTGCGGGTGATTTGCGCCCGCGT
CGATGCTCCAGGAGGATTTC

Downstream 100 bases:

>100_bases
ACGAACGGACTCAGACCGGAGGCGGCGTCGGGGTAATCGCCACGCACAGGCTCAACAGCGCCAGCCAGCCGAATCCCAGC
AAACGTTGCTTGAAGGAGTG

Product: D-lactate dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MRTIVFSSQSYDRDSFLAADCPAGIELHFQPARLSLDTAALAEHHEVVCAFINDDLSAPVLERLAAGGTRLIALRSAGYN
HVDLAVAKRLELAVVRVPAYSPHAVAEHAVALILALNRRLHRAYNRTREGDFSLHGLTGFDLVGKTVGIVGTGQIGATFA
KIMHGFGCELLAYDPYPNPAVEALGARYLSLPDLLAQSRIISLHCPLNEQSRHLINRDSLAHMQPGAMLINTGRGGLVDT
PALIDALKDGQLGYLGLDVYEEEAQLFFEDRSDLPLQDDVLARLLTFPNVIVTAHQAFLTHEALGAIAATTLHNIATWAT
GTPQNQVEG

Sequences:

>Translated_329_residues
MRTIVFSSQSYDRDSFLAADCPAGIELHFQPARLSLDTAALAEHHEVVCAFINDDLSAPVLERLAAGGTRLIALRSAGYN
HVDLAVAKRLELAVVRVPAYSPHAVAEHAVALILALNRRLHRAYNRTREGDFSLHGLTGFDLVGKTVGIVGTGQIGATFA
KIMHGFGCELLAYDPYPNPAVEALGARYLSLPDLLAQSRIISLHCPLNEQSRHLINRDSLAHMQPGAMLINTGRGGLVDT
PALIDALKDGQLGYLGLDVYEEEAQLFFEDRSDLPLQDDVLARLLTFPNVIVTAHQAFLTHEALGAIAATTLHNIATWAT
GTPQNQVEG
>Mature_329_residues
MRTIVFSSQSYDRDSFLAADCPAGIELHFQPARLSLDTAALAEHHEVVCAFINDDLSAPVLERLAAGGTRLIALRSAGYN
HVDLAVAKRLELAVVRVPAYSPHAVAEHAVALILALNRRLHRAYNRTREGDFSLHGLTGFDLVGKTVGIVGTGQIGATFA
KIMHGFGCELLAYDPYPNPAVEALGARYLSLPDLLAQSRIISLHCPLNEQSRHLINRDSLAHMQPGAMLINTGRGGLVDT
PALIDALKDGQLGYLGLDVYEEEAQLFFEDRSDLPLQDDVLARLLTFPNVIVTAHQAFLTHEALGAIAATTLHNIATWAT
GTPQNQVEG

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=252, Percent_Identity=38.0952380952381, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4557497, Length=252, Percent_Identity=38.0952380952381, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI145580578, Length=252, Percent_Identity=36.5079365079365, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4557499, Length=252, Percent_Identity=36.5079365079365, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI145580575, Length=252, Percent_Identity=36.5079365079365, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI23308577, Length=240, Percent_Identity=31.6666666666667, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI6912396, Length=313, Percent_Identity=29.3929712460064, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1787645, Length=321, Percent_Identity=51.0903426791277, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI87082289, Length=269, Percent_Identity=31.9702602230483, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789279, Length=243, Percent_Identity=31.2757201646091, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788660, Length=210, Percent_Identity=30.4761904761905, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=300, Percent_Identity=27, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=258, Percent_Identity=29.0697674418605, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=199, Percent_Identity=34.1708542713568, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6320925, Length=232, Percent_Identity=32.7586206896552, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322116, Length=232, Percent_Identity=32.3275862068966, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=29.7169811320755, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=30.7142857142857, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321253, Length=135, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325144, Length=127, Percent_Identity=31.496062992126, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24646446, Length=272, Percent_Identity=37.1323529411765, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24646448, Length=272, Percent_Identity=37.1323529411765, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24646452, Length=272, Percent_Identity=37.1323529411765, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24646450, Length=272, Percent_Identity=37.1323529411765, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI62472511, Length=253, Percent_Identity=38.3399209486166, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19921140, Length=245, Percent_Identity=29.3877551020408, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45552429, Length=270, Percent_Identity=29.2592592592593, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585514, Length=270, Percent_Identity=29.2592592592593, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28574282, Length=270, Percent_Identity=29.2592592592593, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28574284, Length=270, Percent_Identity=29.2592592592593, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI45551003, Length=270, Percent_Identity=29.2592592592593, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28574286, Length=270, Percent_Identity=29.6296296296296, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28571528, Length=274, Percent_Identity=31.021897810219, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585516, Length=267, Percent_Identity=25.0936329588015, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 35578; Mature: 35578

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIVFSSQSYDRDSFLAADCPAGIELHFQPARLSLDTAALAEHHEVVCAFINDDLSAPV
CCEEEECCCCCCCCCEEEECCCCCCEEEEECCCEECHHHHHHHHHEEEEEEECCCCCHHH
LERLAAGGTRLIALRSAGYNHVDLAVAKRLELAVVRVPAYSPHAVAEHAVALILALNRRL
HHHHHCCCEEEEEEECCCCCCHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRAYNRTREGDFSLHGLTGFDLVGKTVGIVGTGQIGATFAKIMHGFGCELLAYDPYPNPA
HHHHHCCCCCCEEEECCCCHHHHCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHH
VEALGARYLSLPDLLAQSRIISLHCPLNEQSRHLINRDSLAHMQPGAMLINTGRGGLVDT
HHHHHHHHCCCHHHHHCCCEEEEECCCCHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCC
PALIDALKDGQLGYLGLDVYEEEAQLFFEDRSDLPLQDDVLARLLTFPNVIVTAHQAFLT
HHHHHHHCCCCEEEEEEEEEHHHHHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
HEALGAIAATTLHNIATWATGTPQNQVEG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRTIVFSSQSYDRDSFLAADCPAGIELHFQPARLSLDTAALAEHHEVVCAFINDDLSAPV
CCEEEECCCCCCCCCEEEECCCCCCEEEEECCCEECHHHHHHHHHEEEEEEECCCCCHHH
LERLAAGGTRLIALRSAGYNHVDLAVAKRLELAVVRVPAYSPHAVAEHAVALILALNRRL
HHHHHCCCEEEEEEECCCCCCHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
HRAYNRTREGDFSLHGLTGFDLVGKTVGIVGTGQIGATFAKIMHGFGCELLAYDPYPNPA
HHHHHCCCCCCEEEECCCCHHHHCCEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHH
VEALGARYLSLPDLLAQSRIISLHCPLNEQSRHLINRDSLAHMQPGAMLINTGRGGLVDT
HHHHHHHHCCCHHHHHCCCEEEEECCCCHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCC
PALIDALKDGQLGYLGLDVYEEEAQLFFEDRSDLPLQDDVLARLLTFPNVIVTAHQAFLT
HHHHHHHCCCCEEEEEEEEEHHHHHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHH
HEALGAIAATTLHNIATWATGTPQNQVEG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]