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The map label for this gene is kdsB [H]

Identifier: 77460394

GI number: 77460394

Start: 4711200

End: 4711964

Strand: Reverse

Name: kdsB [H]

Synonym: Pfl01_4173

Alternate gene names: 77460394

Gene position: 4711964-4711200 (Counterclockwise)

Preceding gene: 77460395

Following gene: 77460393

Centisome position: 73.19

GC content: 65.49

Gene sequence:

>765_bases
ATGACCACCGCCTTCACCGTTGTCATCCCGTCGCGTTACGCCTCGACCCGTCTGCCGGGCAAGCCGCTGCTGGACATCGC
CGGCAAGCCGATGATCCAGCACGTCTGGGAGCAGGCGAGCAAAAGCAGCGCCAGCCGTGTGGTGGTGGCGACCGACGATG
CGCGGATTGTCGAGGCCTGCAAGGCCTTCGGCGCCGAAGTGGTGCTGACCCGTGAAGATCACAACTCCGGCACCGATCGT
CTGGCTGAAGTGGCTGCCAAGCTGGGTCTGGAGCCGGATGCCATCGTGGTCAACGTGCAGGGTGACGAACCGCTGATCCC
GCCGAGCGTGATCGATCAGGTCGCCGCCAACCTCGCGGCCCACACCGAAGCGCGCATGGCCACCTTGGCCGAGCCGATCG
AAGACGTGGAAACCTTGTTCAACCCGAACGTGGTCAAGGTCGTCAGCGACCTCAACGGTCTGGCGCTGACCTTCAGTCGC
GCGACCCTGCCATTGGCTCGCGATGCGTTCGCCAAGCGCCGCGACGTGCTGCCGGAAGGCGTACCGTATCGTCGCCACAT
CGGCATCTATGCCTACCGCGCCGGTTTCCTTCAGGACTTTGTGAGCTGGGGCCCGTGCTGGCTGGAAAACACCGAATCCC
TCGAACAACTGCGTGCCCTGTGGCACGGCGTGCGGATTCATGTGGCGGACGCGTTGATCGCGCCGCCGACCGGGGTCGAC
ACCGCCGAAGACCTCGAGCGCGTCCGTCGCCTGCTGGAGGCCTGA

Upstream 100 bases:

>100_bases
TCAGCAAGGGCGCCGGTCTGGCGTACCCGATCCGCGACGGCATCCCGGTCATGCTGGAAAGCGAAGCCCGTACCCTGACC
ACCGACGAGCGTCTGGATAA

Downstream 100 bases:

>100_bases
TGCGCGTTCTGTTCGTGTGCCTGGGCAACATCTGCCGTTCGCCCACCGCCGAAGGCGTGTTGCGCCACAAGTTGCGCGAA
GCGGGGCTGGCGGATCAGGT

Product: 3-deoxy-manno-octulosonate cytidylyltransferase

Products: NA

Alternate protein names: CMP-2-keto-3-deoxyoctulosonic acid synthase; CKS; CMP-KDO synthase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTTAFTVVIPSRYASTRLPGKPLLDIAGKPMIQHVWEQASKSSASRVVVATDDARIVEACKAFGAEVVLTREDHNSGTDR
LAEVAAKLGLEPDAIVVNVQGDEPLIPPSVIDQVAANLAAHTEARMATLAEPIEDVETLFNPNVVKVVSDLNGLALTFSR
ATLPLARDAFAKRRDVLPEGVPYRRHIGIYAYRAGFLQDFVSWGPCWLENTESLEQLRALWHGVRIHVADALIAPPTGVD
TAEDLERVRRLLEA

Sequences:

>Translated_254_residues
MTTAFTVVIPSRYASTRLPGKPLLDIAGKPMIQHVWEQASKSSASRVVVATDDARIVEACKAFGAEVVLTREDHNSGTDR
LAEVAAKLGLEPDAIVVNVQGDEPLIPPSVIDQVAANLAAHTEARMATLAEPIEDVETLFNPNVVKVVSDLNGLALTFSR
ATLPLARDAFAKRRDVLPEGVPYRRHIGIYAYRAGFLQDFVSWGPCWLENTESLEQLRALWHGVRIHVADALIAPPTGVD
TAEDLERVRRLLEA
>Mature_253_residues
TTAFTVVIPSRYASTRLPGKPLLDIAGKPMIQHVWEQASKSSASRVVVATDDARIVEACKAFGAEVVLTREDHNSGTDRL
AEVAAKLGLEPDAIVVNVQGDEPLIPPSVIDQVAANLAAHTEARMATLAEPIEDVETLFNPNVVKVVSDLNGLALTFSRA
TLPLARDAFAKRRDVLPEGVPYRRHIGIYAYRAGFLQDFVSWGPCWLENTESLEQLRALWHGVRIHVADALIAPPTGVDT
AEDLERVRRLLEA

Specific function: Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria [H]

COG id: COG1212

COG function: function code M; CMP-2-keto-3-deoxyoctulosonic acid synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsB family [H]

Homologues:

Organism=Escherichia coli, GI1787147, Length=246, Percent_Identity=58.130081300813, Blast_Score=278, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329
- InterPro:   IPR004528 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: =2.7.7.38 [H]

Molecular weight: Translated: 27717; Mature: 27586

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAFTVVIPSRYASTRLPGKPLLDIAGKPMIQHVWEQASKSSASRVVVATDDARIVEAC
CCCEEEEEECCCHHCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
KAFGAEVVLTREDHNSGTDRLAEVAAKLGLEPDAIVVNVQGDEPLIPPSVIDQVAANLAA
HHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
HTEARMATLAEPIEDVETLFNPNVVKVVSDLNGLALTFSRATLPLARDAFAKRRDVLPEG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCC
VPYRRHIGIYAYRAGFLQDFVSWGPCWLENTESLEQLRALWHGVRIHVADALIAPPTGVD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEHHHCCCCCCCC
TAEDLERVRRLLEA
CHHHHHHHHHHHCC
>Mature Secondary Structure 
TTAFTVVIPSRYASTRLPGKPLLDIAGKPMIQHVWEQASKSSASRVVVATDDARIVEAC
CCEEEEEECCCHHCCCCCCCCHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
KAFGAEVVLTREDHNSGTDRLAEVAAKLGLEPDAIVVNVQGDEPLIPPSVIDQVAANLAA
HHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
HTEARMATLAEPIEDVETLFNPNVVKVVSDLNGLALTFSRATLPLARDAFAKRRDVLPEG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCC
VPYRRHIGIYAYRAGFLQDFVSWGPCWLENTESLEQLRALWHGVRIHVADALIAPPTGVD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEHHHCCCCCCCC
TAEDLERVRRLLEA
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA