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The map label for this gene is capD [H]

Identifier: 77460272

GI number: 77460272

Start: 4575106

End: 4577100

Strand: Reverse

Name: capD [H]

Synonym: Pfl01_4051

Alternate gene names: 77460272

Gene position: 4577100-4575106 (Counterclockwise)

Preceding gene: 77460274

Following gene: 77460271

Centisome position: 71.09

GC content: 56.19

Gene sequence:

>1995_bases
ATGGATAAAATGCGAGCATTTCTGCTGGGTTTGCCGCGTCGTCAAAAACGACTGCTGCAGGTGGCCACCGACGTCGTTCT
CGTCTGGGGGGCGCTGTGGCTGGCATTCATCGTTCGCCTCGGAATCGATGATATGTACAACCCCATCAAAGTGCATGCCT
GGCTATTCGTCTGTGCCCCGCTTGTTGCAATACCGCTGTTTATTCGATTTGGCATGTATCGCGCGGTCATGCGCTACTTC
GGCAATGATGCACTAATTGCCATCATCAAGGCGGTCAGCCTTTCTTCACTGATACTCGCAGTAGTTGTCTACTGGTACAG
CAATCATGAAAGTGTTGTCCCCAGATCCATCATTTTCAACTACTGGTGGCTGAGTCTGGTGATCATTGGCGGATTGCGCC
TGTGCATGCGTCAGTATTTCATGGGTGACTGGTTCACCGCAGCCCAGCACGTACCGTTCACCAGTCGCGACGATGGCCTG
ACCAAGGTGGCGATCTATGGTGCCGGAGTCGCCGGTAATCAGTTGGTCGCGGCTCTGCGAATGGGGCGAGTCATGCGCCC
GGTGGCGTTCATCGATGATGATGCGAGTATCGCTGATCGGGCCATTTCGGGTCTGCAGGTCTATAAGCCCAAGCACATTC
AGCAAATGATCGACGTGACGGGAGCGCAGGAAATTCTCCTGGCGCTGCCTTCATCCACTCGTGCCCGTCGCCGGGAAATC
CTCAATTTGCTGGAAGGATTCCCACTGCATATTCGCAGTGTTCCAAGTTTTACCGACCTGGCCAGCGGCCGGGTGAAGGT
CGATGACATTCAGGAAGTCGACATCGCGGACCTGCTGGGGCGCGACTCGGTGCCGGCCCAATCCGATCTGCTGGCTCACT
GCATTACCGGCAAAGTGGTGATGGTGACAGGCGCGGGCGGGTCGATCGGTTCGGAGCTCTGCCGGCAAATCTTTCTGCTT
GGGCCGATAACGCTTCTGCTGTTCGATCACAGTGAGTTCAATCTATACAGCATTCTGTCCGAGCTCGAACAGCGCATCGC
GCGCGAGTCTGTCTCCATTCGTCTACTGCCGATTCTCGGTTCCATCCGGCATCCTCACAAGCTCCTGGATGTCATGAAGA
CGTGGGGCGTGGACACTGTCTATCACGCGGCCGCCTATAAACATGTGCCCATGGTTGAACACAATATTGCCGAAGGTGTG
CTGAATAACGTGATCGGCACCCTCAATACGGCGCAGGCAGCACTGCAGGCAGGTGTTTCCAACTTTGTTCTGATTTCCAC
CGACAAGGCTGTGCGCCCCACCAATATCATGGGCAGCACCAAGCGACTTGCCGAGCTGACCCTGCAGGCGCTGAGTCGTG
AGCTGGCCCCTGTACTGTTCGGCGACAAGGCCAATGTGTCTCGCGTCAACAAGACGCGATTCACCATGGTGCGTTTCGGA
AATGTGCTGGGTTCGTCGGGGTCGGTGATTCCGCTGTTCCATAGCCAGATCAAATCCGGCGGTCCGCTGACGGTCACTCA
TCCCAAGATCACCCGCTATTTCATGACGATCCCGGAAGCCGCGCAGTTGGTGATCCAGGCCGGCTCCATGGGGCAGGGGG
GAGATGTCTTTGTGCTGGACATGGGAGAGCCGGTCAAAATTGTCGAGCTCGCTGAAAAGATGATTCACCTGTCCGGCCTC
AGTGTGCGTTCGGAAAAGAATGTGCACGGCGATATTGCAATCGAATTCACCGGGTTGCGGCCAGGGGAGAAGCTCTATGA
AGAACTCCTGATCGGTGACAATGTTTCGGCGACGCCTCACCCCATGATCATGAGTGCCAACGAGGACCTCATTCCCTGGG
ACGTGCTCAAGGTTCGGCTGTCGGAATTGCTGGCTGCAGTCGAAAAGGACGACTACTCCCGCGTTCGGCAGATTTTGCGC
GAAACCGTCAGTGGCTATGCGCCTGATGGAGAAATTGTCGACTGGATCTATCAGCAGCGTCGCCTCGACCCTTGA

Upstream 100 bases:

>100_bases
GCGCCGGCCGGTCACTTGGCAGTCGTATTCTCTGGTTGAAGGAGGATACAATGCAGGTTGGTTTATAGTTCATGGAGTGG
GACGGTGATCGGGCGATTCA

Downstream 100 bases:

>100_bases
ACGTGCGCGTGTGAGTAAAGAACGCGTTGCGAGTGATAGCTGATCCGGCCTGTAAGCGTCATTGCCAGCCGCCCGGTTGG
TGGAGCCCCAACCGGGCGGC

Product: polysaccharide biosynthesis protein CapD

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MDKMRAFLLGLPRRQKRLLQVATDVVLVWGALWLAFIVRLGIDDMYNPIKVHAWLFVCAPLVAIPLFIRFGMYRAVMRYF
GNDALIAIIKAVSLSSLILAVVVYWYSNHESVVPRSIIFNYWWLSLVIIGGLRLCMRQYFMGDWFTAAQHVPFTSRDDGL
TKVAIYGAGVAGNQLVAALRMGRVMRPVAFIDDDASIADRAISGLQVYKPKHIQQMIDVTGAQEILLALPSSTRARRREI
LNLLEGFPLHIRSVPSFTDLASGRVKVDDIQEVDIADLLGRDSVPAQSDLLAHCITGKVVMVTGAGGSIGSELCRQIFLL
GPITLLLFDHSEFNLYSILSELEQRIARESVSIRLLPILGSIRHPHKLLDVMKTWGVDTVYHAAAYKHVPMVEHNIAEGV
LNNVIGTLNTAQAALQAGVSNFVLISTDKAVRPTNIMGSTKRLAELTLQALSRELAPVLFGDKANVSRVNKTRFTMVRFG
NVLGSSGSVIPLFHSQIKSGGPLTVTHPKITRYFMTIPEAAQLVIQAGSMGQGGDVFVLDMGEPVKIVELAEKMIHLSGL
SVRSEKNVHGDIAIEFTGLRPGEKLYEELLIGDNVSATPHPMIMSANEDLIPWDVLKVRLSELLAAVEKDDYSRVRQILR
ETVSGYAPDGEIVDWIYQQRRLDP

Sequences:

>Translated_664_residues
MDKMRAFLLGLPRRQKRLLQVATDVVLVWGALWLAFIVRLGIDDMYNPIKVHAWLFVCAPLVAIPLFIRFGMYRAVMRYF
GNDALIAIIKAVSLSSLILAVVVYWYSNHESVVPRSIIFNYWWLSLVIIGGLRLCMRQYFMGDWFTAAQHVPFTSRDDGL
TKVAIYGAGVAGNQLVAALRMGRVMRPVAFIDDDASIADRAISGLQVYKPKHIQQMIDVTGAQEILLALPSSTRARRREI
LNLLEGFPLHIRSVPSFTDLASGRVKVDDIQEVDIADLLGRDSVPAQSDLLAHCITGKVVMVTGAGGSIGSELCRQIFLL
GPITLLLFDHSEFNLYSILSELEQRIARESVSIRLLPILGSIRHPHKLLDVMKTWGVDTVYHAAAYKHVPMVEHNIAEGV
LNNVIGTLNTAQAALQAGVSNFVLISTDKAVRPTNIMGSTKRLAELTLQALSRELAPVLFGDKANVSRVNKTRFTMVRFG
NVLGSSGSVIPLFHSQIKSGGPLTVTHPKITRYFMTIPEAAQLVIQAGSMGQGGDVFVLDMGEPVKIVELAEKMIHLSGL
SVRSEKNVHGDIAIEFTGLRPGEKLYEELLIGDNVSATPHPMIMSANEDLIPWDVLKVRLSELLAAVEKDDYSRVRQILR
ETVSGYAPDGEIVDWIYQQRRLDP
>Mature_664_residues
MDKMRAFLLGLPRRQKRLLQVATDVVLVWGALWLAFIVRLGIDDMYNPIKVHAWLFVCAPLVAIPLFIRFGMYRAVMRYF
GNDALIAIIKAVSLSSLILAVVVYWYSNHESVVPRSIIFNYWWLSLVIIGGLRLCMRQYFMGDWFTAAQHVPFTSRDDGL
TKVAIYGAGVAGNQLVAALRMGRVMRPVAFIDDDASIADRAISGLQVYKPKHIQQMIDVTGAQEILLALPSSTRARRREI
LNLLEGFPLHIRSVPSFTDLASGRVKVDDIQEVDIADLLGRDSVPAQSDLLAHCITGKVVMVTGAGGSIGSELCRQIFLL
GPITLLLFDHSEFNLYSILSELEQRIARESVSIRLLPILGSIRHPHKLLDVMKTWGVDTVYHAAAYKHVPMVEHNIAEGV
LNNVIGTLNTAQAALQAGVSNFVLISTDKAVRPTNIMGSTKRLAELTLQALSRELAPVLFGDKANVSRVNKTRFTMVRFG
NVLGSSGSVIPLFHSQIKSGGPLTVTHPKITRYFMTIPEAAQLVIQAGSMGQGGDVFVLDMGEPVKIVELAEKMIHLSGL
SVRSEKNVHGDIAIEFTGLRPGEKLYEELLIGDNVSATPHPMIMSANEDLIPWDVLKVRLSELLAAVEKDDYSRVRQILR
ETVSGYAPDGEIVDWIYQQRRLDP

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 73546; Mature: 73546

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKMRAFLLGLPRRQKRLLQVATDVVLVWGALWLAFIVRLGIDDMYNPIKVHAWLFVCAP
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHH
LVAIPLFIRFGMYRAVMRYFGNDALIAIIKAVSLSSLILAVVVYWYSNHESVVPRSIIFN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
YWWLSLVIIGGLRLCMRQYFMGDWFTAAQHVPFTSRDDGLTKVAIYGAGVAGNQLVAALR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
MGRVMRPVAFIDDDASIADRAISGLQVYKPKHIQQMIDVTGAQEILLALPSSTRARRREI
HHHHHCCCEEECCCHHHHHHHHCCCHHCCHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHH
LNLLEGFPLHIRSVPSFTDLASGRVKVDDIQEVDIADLLGRDSVPAQSDLLAHCITGKVV
HHHHCCCCEEEECCCCHHHHCCCCEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCEE
MVTGAGGSIGSELCRQIFLLGPITLLLFDHSEFNLYSILSELEQRIARESVSIRLLPILG
EEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEHHC
SIRHPHKLLDVMKTWGVDTVYHAAAYKHVPMVEHNIAEGVLNNVIGTLNTAQAALQAGVS
CCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NFVLISTDKAVRPTNIMGSTKRLAELTLQALSRELAPVLFGDKANVSRVNKTRFTMVRFG
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHH
NVLGSSGSVIPLFHSQIKSGGPLTVTHPKITRYFMTIPEAAQLVIQAGSMGQGGDVFVLD
HHHCCCCCEEHHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEE
MGEPVKIVELAEKMIHLSGLSVRSEKNVHGDIAIEFTGLRPGEKLYEELLIGDNVSATPH
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCC
PMIMSANEDLIPWDVLKVRLSELLAAVEKDDYSRVRQILRETVSGYAPDGEIVDWIYQQR
CEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RLDP
CCCC
>Mature Secondary Structure
MDKMRAFLLGLPRRQKRLLQVATDVVLVWGALWLAFIVRLGIDDMYNPIKVHAWLFVCAP
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHH
LVAIPLFIRFGMYRAVMRYFGNDALIAIIKAVSLSSLILAVVVYWYSNHESVVPRSIIFN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
YWWLSLVIIGGLRLCMRQYFMGDWFTAAQHVPFTSRDDGLTKVAIYGAGVAGNQLVAALR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
MGRVMRPVAFIDDDASIADRAISGLQVYKPKHIQQMIDVTGAQEILLALPSSTRARRREI
HHHHHCCCEEECCCHHHHHHHHCCCHHCCHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHH
LNLLEGFPLHIRSVPSFTDLASGRVKVDDIQEVDIADLLGRDSVPAQSDLLAHCITGKVV
HHHHCCCCEEEECCCCHHHHCCCCEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCEE
MVTGAGGSIGSELCRQIFLLGPITLLLFDHSEFNLYSILSELEQRIARESVSIRLLPILG
EEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEHHC
SIRHPHKLLDVMKTWGVDTVYHAAAYKHVPMVEHNIAEGVLNNVIGTLNTAQAALQAGVS
CCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NFVLISTDKAVRPTNIMGSTKRLAELTLQALSRELAPVLFGDKANVSRVNKTRFTMVRFG
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHH
NVLGSSGSVIPLFHSQIKSGGPLTVTHPKITRYFMTIPEAAQLVIQAGSMGQGGDVFVLD
HHHCCCCCEEHHHHHHHCCCCCEEEECHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEE
MGEPVKIVELAEKMIHLSGLSVRSEKNVHGDIAIEFTGLRPGEKLYEELLIGDNVSATPH
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCC
PMIMSANEDLIPWDVLKVRLSELLAAVEKDDYSRVRQILRETVSGYAPDGEIVDWIYQQR
CEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RLDP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]