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The map label for this gene is epsM [H]

Identifier: 77460266

GI number: 77460266

Start: 4569822

End: 4570490

Strand: Reverse

Name: epsM [H]

Synonym: Pfl01_4045

Alternate gene names: 77460266

Gene position: 4570490-4569822 (Counterclockwise)

Preceding gene: 77460267

Following gene: 77460265

Centisome position: 70.99

GC content: 58.59

Gene sequence:

>669_bases
ATGAAAAAGTATTACGGAATCATCGGTGCAGGAGGTTATGGCAGAGAGGTCATTCCCCTGGCGCGTGCTTCTCTTTCAGC
ATCGCTTGCAGGCGGTGACGCGGAGCTGTTTTTCGTTGTTGAGGGCGAGGGTTCGGCCGAAACGGTCAATGGCTTTCCGG
TGGTGAGCATGGATGCGTTCCTGGCGCTGTCCGGGGAGCGTTATTTCAGTATCGCTATTGGTGCTTCCGATGTCAGGGAG
CGTATCGCCCGGCTGTGCGAAGAACAGGGCGCGATGCCTTTTTCCGTGATTGCTCAAAACGTCGTGATCCTCGATGCCAA
TGAAATCGGTGAGGGGGCGGTGCTGTCCCCGTTCGTGACCGTGACGTCGAACGCGAAGATCGGCAAACATTTTCACGCCA
ACCTCTATTCCTATGTGGCGCACGACTGTGTGGTCGGCGACTTCGTGACCTTTGCCCCTGGCGTCAAATGTAACGGCAAT
GTCGTCATCGAAGATCATGCCTACATCGGTACTGGCGCCGTGCTCAAGCAAGGCACGTCCAAGCGACCCTTGACGATCGG
GCGTGGGGCCATTGTCGGGATGGGGGCCGTGGTGACCAAGAGTGTCGCGCCAGGCGACACGGTCATCGGCAACCCCGCAC
GGCCTCTGCGCGAGGCGCTGCGCAAATGA

Upstream 100 bases:

>100_bases
CGCTCTACCACACCCTGTCGCGGGAAGAGATCGACATGATCTGCCGACTCATCAAGCGCTCTGTCAGGTATTGAGTGAAT
TTTCGGATCAGGAGATTTTG

Downstream 100 bases:

>100_bases
CAACGCTGAATGATTTTCTGAGCGGGCTCTCCATCGAGCAGATTGAAGTCGGGATGCAGGTTTCCTATTCCCAGACCATC
ACGGATGCCGATGTGAAAGC

Product: hexapaptide repeat-containing transferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKKYYGIIGAGGYGREVIPLARASLSASLAGGDAELFFVVEGEGSAETVNGFPVVSMDAFLALSGERYFSIAIGASDVRE
RIARLCEEQGAMPFSVIAQNVVILDANEIGEGAVLSPFVTVTSNAKIGKHFHANLYSYVAHDCVVGDFVTFAPGVKCNGN
VVIEDHAYIGTGAVLKQGTSKRPLTIGRGAIVGMGAVVTKSVAPGDTVIGNPARPLREALRK

Sequences:

>Translated_222_residues
MKKYYGIIGAGGYGREVIPLARASLSASLAGGDAELFFVVEGEGSAETVNGFPVVSMDAFLALSGERYFSIAIGASDVRE
RIARLCEEQGAMPFSVIAQNVVILDANEIGEGAVLSPFVTVTSNAKIGKHFHANLYSYVAHDCVVGDFVTFAPGVKCNGN
VVIEDHAYIGTGAVLKQGTSKRPLTIGRGAIVGMGAVVTKSVAPGDTVIGNPARPLREALRK
>Mature_222_residues
MKKYYGIIGAGGYGREVIPLARASLSASLAGGDAELFFVVEGEGSAETVNGFPVVSMDAFLALSGERYFSIAIGASDVRE
RIARLCEEQGAMPFSVIAQNVVILDANEIGEGAVLSPFVTVTSNAKIGKHFHANLYSYVAHDCVVGDFVTFAPGVKCNGN
VVIEDHAYIGTGAVLKQGTSKRPLTIGRGAIVGMGAVVTKSVAPGDTVIGNPARPLREALRK

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 23162; Mature: 23162

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYYGIIGAGGYGREVIPLARASLSASLAGGDAELFFVVEGEGSAETVNGFPVVSMDAF
CCCEEEEEECCCCCCEEHHHHHHHHHHEECCCCEEEEEEEECCCCCEEECCCEEEEEEEE
LALSGERYFSIAIGASDVRERIARLCEEQGAMPFSVIAQNVVILDANEIGEGAVLSPFVT
EEECCCEEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHCEEEEEECCCCCCCCEECCEEE
VTSNAKIGKHFHANLYSYVAHDCVVGDFVTFAPGVKCNGNVVIEDHAYIGTGAVLKQGTS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEECCCHHHHCCCC
KRPLTIGRGAIVGMGAVVTKSVAPGDTVIGNPARPLREALRK
CCCEEECCCCEEECCHHHCCCCCCCCCEECCCCHHHHHHHCC
>Mature Secondary Structure
MKKYYGIIGAGGYGREVIPLARASLSASLAGGDAELFFVVEGEGSAETVNGFPVVSMDAF
CCCEEEEEECCCCCCEEHHHHHHHHHHEECCCCEEEEEEEECCCCCEEECCCEEEEEEEE
LALSGERYFSIAIGASDVRERIARLCEEQGAMPFSVIAQNVVILDANEIGEGAVLSPFVT
EEECCCEEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHCEEEEEECCCCCCCCEECCEEE
VTSNAKIGKHFHANLYSYVAHDCVVGDFVTFAPGVKCNGNVVIEDHAYIGTGAVLKQGTS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEECCCHHHHCCCC
KRPLTIGRGAIVGMGAVVTKSVAPGDTVIGNPARPLREALRK
CCCEEECCCCEEECCHHHCCCCCCCCCEECCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]