Click here to switch to the map view.

The map label for this gene is mltF [H]

Identifier: 77460241

GI number: 77460241

Start: 4544241

End: 4545662

Strand: Reverse

Name: mltF [H]

Synonym: Pfl01_4020

Alternate gene names: 77460241

Gene position: 4545662-4544241 (Counterclockwise)

Preceding gene: 77460245

Following gene: 77460240

Centisome position: 70.6

GC content: 62.94

Gene sequence:

>1422_bases
ATGGTTCGTCCCTCGGTTTTGCTCCTGCTGTGTGGTTCGCTGCTGCTGCCGATGACGGCGGTCGCGCGGCTGCCCGGACC
GCTGCAAGCCGTGCCGGCCGCCAAGGTCCGCGACCTGACCGAGATCCGCAGCAGCCGCGTGTTGCGGGTGCTGGTCAACC
AGAGCCGCAACAGCTCCGGCGAAGTCCAGGGCCAGGCCATCGGCATCGAATACCACCGTCTGCGCGCCTTCGAGCAATAC
CTCAATGGCCACGCTCGCGACGGCCAGGAAATCACCCTCAAGATCATCCCCAAAGCCAAGGATCAATTGCTCGGTGCGTT
GCAGCGCGGCGAAGGCGATCTTGTGGCGCCGGGCGAACTGCTCGAGCTGCAACCGGGCCACGCGGTTGCCAGCAGCGAGC
CGATCGCCAGCAACGTGCCGCTGGTGCTGGTTGGCATCAAGGGTGAAAAGCGCTACACCAAGGTCGAGCAACTGGCCGGC
AAAACCCTGGCGCTGCCCACCGGCAGTGCGGCGGGGGAGGCGGTCAGTCAGCTCAACCAGAAGCTGGCGCTGCACAAACT
GGCGCCGATCAGGATCGAATGGGTCGATCCGACCCTTGCGGTCGAAGACGTGCTGGAGATGGTTCAGGGCGGGATTTTCC
ACCTGACCATTGTCGAGCAACCGATTGCCGAGCGGTGGGGCAAGATCCTGCCCAAGCTGCGTTTCGACCGTCAGGTGATG
ATCAGCGAACCGGGCGAGGAATATTGGTTCGTGCGCCGTGATGCGTCGATGTTGCGGGCCAGCATCGACCGCTTCCTGAC
CGGCTACAAAAAACCATCGAACGAAGATGCGGCGTTCCTGCGGATCTACCGCCGCCTCTATCAAGTGCACTATCCGTTGG
CCAAGGCTGACCGTCAGCGTCTGGAAAAACTGCGCCCGACCCTGCAGAAACACGCCGAAGCGCAAAACATGGACTGGCTC
AACCTGGCGGCGCTGGCCTTCAAGGAGTCGGCGCTGCAGCCCAACGCGCGCAGCGGCAGCGGGCCGACCGGGCTGATGCA
GATCACCCCGTCCGCGGCACAGCGGGTTGGCGTCAACAACATCCAGAATCTCGATGCGAATGTGCAGGCCGGGGCCAAGT
ACCTGGCGATGATCCGCCGCAAGTTCTTCAACAGTCCGAAACTCAATGAGCGCGAGCGCATGGCGTTCACTCTGGCGGCT
TACAACATCGGCCCGGAACGGGTGCAAGGCATGCGTGCCGAGGCGCGGCGTCGCGGCCTGAATCCCAACCAATGGTTCTT
TCAGGTCGAGCGCATCGCCATGGAGCAGGTGGGAATGGGCGCCGTCAGCTATGTTAATAGCGTGAACAAGTATTACTTGG
CGTTCGACCGGGAGCGGGAGTCGTTGGAGCCCGGGGGGCAGAAAGTTGTCTCACGCAAATGA

Upstream 100 bases:

>100_bases
CAGTCTCTCGCACTTTATCAGCGCTCCAGGCGCTCTGTTTTCGTCGGTGTTTTGCGACCGCTCAGCGGTGAAAACGCCCC
GTATTCTTTCCGGAGAGTGG

Downstream 100 bases:

>100_bases
TCAAATAAGTTGATTGTTATGGCGGAATTTTTGCGCTTTTAACATGAAAATAACTGATTAATATAGCGGCCAACCAACAA
GCACTTCTGACAACAAGGAA

Product: lytic transglycosylase catalytic subunit

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MVRPSVLLLLCGSLLLPMTAVARLPGPLQAVPAAKVRDLTEIRSSRVLRVLVNQSRNSSGEVQGQAIGIEYHRLRAFEQY
LNGHARDGQEITLKIIPKAKDQLLGALQRGEGDLVAPGELLELQPGHAVASSEPIASNVPLVLVGIKGEKRYTKVEQLAG
KTLALPTGSAAGEAVSQLNQKLALHKLAPIRIEWVDPTLAVEDVLEMVQGGIFHLTIVEQPIAERWGKILPKLRFDRQVM
ISEPGEEYWFVRRDASMLRASIDRFLTGYKKPSNEDAAFLRIYRRLYQVHYPLAKADRQRLEKLRPTLQKHAEAQNMDWL
NLAALAFKESALQPNARSGSGPTGLMQITPSAAQRVGVNNIQNLDANVQAGAKYLAMIRRKFFNSPKLNERERMAFTLAA
YNIGPERVQGMRAEARRRGLNPNQWFFQVERIAMEQVGMGAVSYVNSVNKYYLAFDRERESLEPGGQKVVSRK

Sequences:

>Translated_473_residues
MVRPSVLLLLCGSLLLPMTAVARLPGPLQAVPAAKVRDLTEIRSSRVLRVLVNQSRNSSGEVQGQAIGIEYHRLRAFEQY
LNGHARDGQEITLKIIPKAKDQLLGALQRGEGDLVAPGELLELQPGHAVASSEPIASNVPLVLVGIKGEKRYTKVEQLAG
KTLALPTGSAAGEAVSQLNQKLALHKLAPIRIEWVDPTLAVEDVLEMVQGGIFHLTIVEQPIAERWGKILPKLRFDRQVM
ISEPGEEYWFVRRDASMLRASIDRFLTGYKKPSNEDAAFLRIYRRLYQVHYPLAKADRQRLEKLRPTLQKHAEAQNMDWL
NLAALAFKESALQPNARSGSGPTGLMQITPSAAQRVGVNNIQNLDANVQAGAKYLAMIRRKFFNSPKLNERERMAFTLAA
YNIGPERVQGMRAEARRRGLNPNQWFFQVERIAMEQVGMGAVSYVNSVNKYYLAFDRERESLEPGGQKVVSRK
>Mature_473_residues
MVRPSVLLLLCGSLLLPMTAVARLPGPLQAVPAAKVRDLTEIRSSRVLRVLVNQSRNSSGEVQGQAIGIEYHRLRAFEQY
LNGHARDGQEITLKIIPKAKDQLLGALQRGEGDLVAPGELLELQPGHAVASSEPIASNVPLVLVGIKGEKRYTKVEQLAG
KTLALPTGSAAGEAVSQLNQKLALHKLAPIRIEWVDPTLAVEDVLEMVQGGIFHLTIVEQPIAERWGKILPKLRFDRQVM
ISEPGEEYWFVRRDASMLRASIDRFLTGYKKPSNEDAAFLRIYRRLYQVHYPLAKADRQRLEKLRPTLQKHAEAQNMDWL
NLAALAFKESALQPNARSGSGPTGLMQITPSAAQRVGVNNIQNLDANVQAGAKYLAMIRRKFFNSPKLNERERMAFTLAA
YNIGPERVQGMRAEARRRGLNPNQWFFQVERIAMEQVGMGAVSYVNSVNKYYLAFDRERESLEPGGQKVVSRK

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=456, Percent_Identity=25, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 52778; Mature: 52778

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRPSVLLLLCGSLLLPMTAVARLPGPLQAVPAAKVRDLTEIRSSRVLRVLVNQSRNSSG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
EVQGQAIGIEYHRLRAFEQYLNGHARDGQEITLKIIPKAKDQLLGALQRGEGDLVAPGEL
CCCCEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEECCCHH
LELQPGHAVASSEPIASNVPLVLVGIKGEKRYTKVEQLAGKTLALPTGSAAGEAVSQLNQ
EEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHH
KLALHKLAPIRIEWVDPTLAVEDVLEMVQGGIFHLTIVEQPIAERWGKILPKLRFDRQVM
HHHHHHCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCHHCCCCEEE
ISEPGEEYWFVRRDASMLRASIDRFLTGYKKPSNEDAAFLRIYRRLYQVHYPLAKADRQR
ECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
LEKLRPTLQKHAEAQNMDWLNLAALAFKESALQPNARSGSGPTGLMQITPSAAQRVGVNN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHCCCCC
IQNLDANVQAGAKYLAMIRRKFFNSPKLNERERMAFTLAAYNIGPERVQGMRAEARRRGL
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEEECCCHHHHHHHHHHHHHCCC
NPNQWFFQVERIAMEQVGMGAVSYVNSVNKYYLAFDRERESLEPGGQKVVSRK
CCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHCCCCCHHHCCCC
>Mature Secondary Structure
MVRPSVLLLLCGSLLLPMTAVARLPGPLQAVPAAKVRDLTEIRSSRVLRVLVNQSRNSSG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
EVQGQAIGIEYHRLRAFEQYLNGHARDGQEITLKIIPKAKDQLLGALQRGEGDLVAPGEL
CCCCEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEECCCHH
LELQPGHAVASSEPIASNVPLVLVGIKGEKRYTKVEQLAGKTLALPTGSAAGEAVSQLNQ
EEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHHH
KLALHKLAPIRIEWVDPTLAVEDVLEMVQGGIFHLTIVEQPIAERWGKILPKLRFDRQVM
HHHHHHCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCHHCCCCEEE
ISEPGEEYWFVRRDASMLRASIDRFLTGYKKPSNEDAAFLRIYRRLYQVHYPLAKADRQR
ECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
LEKLRPTLQKHAEAQNMDWLNLAALAFKESALQPNARSGSGPTGLMQITPSAAQRVGVNN
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHCCCCC
IQNLDANVQAGAKYLAMIRRKFFNSPKLNERERMAFTLAAYNIGPERVQGMRAEARRRGL
HHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEEECCCHHHHHHHHHHHHHCCC
NPNQWFFQVERIAMEQVGMGAVSYVNSVNKYYLAFDRERESLEPGGQKVVSRK
CCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCHHHCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA