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The map label for this gene is ybiS [H]

Identifier: 77460233

GI number: 77460233

Start: 4537326

End: 4538297

Strand: Reverse

Name: ybiS [H]

Synonym: Pfl01_4012

Alternate gene names: 77460233

Gene position: 4538297-4537326 (Counterclockwise)

Preceding gene: 77460234

Following gene: 77460231

Centisome position: 70.49

GC content: 62.24

Gene sequence:

>972_bases
ATGTTGCCGCGTTTTCCTGCCGTCACCCGCTGCCTGTCTCTTGCCGCCCTGTGTGTGGCCGGTCCCGTTGCCGCACTGGA
GTTTCCACTGCCACCGCCTGGTGAAGACATCATCGGCCAGGTGCAGGTGATCAAGGCCAAGTACGAAGACACCTTTGCCG
ATCTGGGCACCACCTACGATCTGGGTTATTCGGAAATGGTCGCGGCCAACCCGGGCGTCGATGCCTGGTTGCCGGGCGCC
GGCACCGAAGTGGTCCTGCCGACCCGTTTCATCCTGCCGCCGGGGCCGCGTGAAGGCATCGTGATCAACCTGGCCGAGTA
CCGCCTCTATTACTACCCGAAAGGCCGGAACGTGGTGTACACCTTCCCGCTGGGTATCGGCCGTGAAGGCTGGGGCTCGC
CGATCGCTCACACCACGATTACCGCGAAGACGCCGAACCCGACCTGGACGCCGCCAGCTTCGATCAAGGCTGAACATGCT
GCCGATGGCGATCCGCTGCCGAACGTGGTGCCGGCCGGTCCGGACAACCCGCTGGGGCCGTTCAAGTTCACCCTGGGCAC
GCCGGGCTACCTGATCCACGGCTCGAACAAGAAGTTCGGTATCGGCATGCGCACCAGTCACGGCTGCTTCCGCATGTTCA
ACAACAACGTGCTGGAAATGGCCAGCATGGTTCCGGTGGGGACTTCGGTACGGATTCTCAACGACGCCTACAAGTTCGGT
CGCAGTGGCGGCAAGATCTATCTGGAGGCGCACACGCCGATCGATGACAAAGGCAACCCGTCGGTGGTCGACAAGCACAC
CGCCGTGATCAACGCGATGCTCAAGCGCGAAGACATCACCAACAACCTTCGCATGAACTGGGATGTGGTGCGTGATGTGG
TGGCGGCCGAAGATGGCCTGCCAACCGAAATCGGCACACCGAACACCTCGGCGCCGATGGTCTCCAGCGCACCGATCGAC
CTGCAGCAATAA

Upstream 100 bases:

>100_bases
AGAGTGATGCCAGCCCATCGGCGCATCTCCCCGAATTCTCTGGGCGCGGTGTACAATCGCGTTCTTATTACCCTTGTCGA
TCTTGCGAAGGATTCTCCGG

Downstream 100 bases:

>100_bases
GCTGGAAGACAAAACCCGCCGAGGCTTGCGCCGTGGCGGGTTTTTTATTGCCCGCAGAAAACTGCAGGCACAAAAAAGCC
GACCCGAAAAACGGGTCGGC

Product: ErfK/YbiS/YcfS/YnhG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MLPRFPAVTRCLSLAALCVAGPVAALEFPLPPPGEDIIGQVQVIKAKYEDTFADLGTTYDLGYSEMVAANPGVDAWLPGA
GTEVVLPTRFILPPGPREGIVINLAEYRLYYYPKGRNVVYTFPLGIGREGWGSPIAHTTITAKTPNPTWTPPASIKAEHA
ADGDPLPNVVPAGPDNPLGPFKFTLGTPGYLIHGSNKKFGIGMRTSHGCFRMFNNNVLEMASMVPVGTSVRILNDAYKFG
RSGGKIYLEAHTPIDDKGNPSVVDKHTAVINAMLKREDITNNLRMNWDVVRDVVAAEDGLPTEIGTPNTSAPMVSSAPID
LQQ

Sequences:

>Translated_323_residues
MLPRFPAVTRCLSLAALCVAGPVAALEFPLPPPGEDIIGQVQVIKAKYEDTFADLGTTYDLGYSEMVAANPGVDAWLPGA
GTEVVLPTRFILPPGPREGIVINLAEYRLYYYPKGRNVVYTFPLGIGREGWGSPIAHTTITAKTPNPTWTPPASIKAEHA
ADGDPLPNVVPAGPDNPLGPFKFTLGTPGYLIHGSNKKFGIGMRTSHGCFRMFNNNVLEMASMVPVGTSVRILNDAYKFG
RSGGKIYLEAHTPIDDKGNPSVVDKHTAVINAMLKREDITNNLRMNWDVVRDVVAAEDGLPTEIGTPNTSAPMVSSAPID
LQQ
>Mature_323_residues
MLPRFPAVTRCLSLAALCVAGPVAALEFPLPPPGEDIIGQVQVIKAKYEDTFADLGTTYDLGYSEMVAANPGVDAWLPGA
GTEVVLPTRFILPPGPREGIVINLAEYRLYYYPKGRNVVYTFPLGIGREGWGSPIAHTTITAKTPNPTWTPPASIKAEHA
ADGDPLPNVVPAGPDNPLGPFKFTLGTPGYLIHGSNKKFGIGMRTSHGCFRMFNNNVLEMASMVPVGTSVRILNDAYKFG
RSGGKIYLEAHTPIDDKGNPSVVDKHTAVINAMLKREDITNNLRMNWDVVRDVVAAEDGLPTEIGTPNTSAPMVSSAPID
LQQ

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=253, Percent_Identity=45.0592885375494, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1788299, Length=303, Percent_Identity=36.6336633663366, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1787356, Length=283, Percent_Identity=37.4558303886926, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1787968, Length=301, Percent_Identity=35.5481727574751, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 34705; Mature: 34705

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPRFPAVTRCLSLAALCVAGPVAALEFPLPPPGEDIIGQVQVIKAKYEDTFADLGTTYD
CCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHCEEEEEEEHHHHHHHHCCCEEC
LGYSEMVAANPGVDAWLPGAGTEVVLPTRFILPPGPREGIVINLAEYRLYYYPKGRNVVY
CCCHHEEECCCCCCEECCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEEEEEECCCCEEE
TFPLGIGREGWGSPIAHTTITAKTPNPTWTPPASIKAEHAADGDPLPNVVPAGPDNPLGP
EECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
FKFTLGTPGYLIHGSNKKFGIGMRTSHGCFRMFNNNVLEMASMVPVGTSVRILNDAYKFG
EEEEECCCCEEEECCCCEEEEECCCCCCHHHHHCCCHHHHHHHCCCCCEEEEEHHHHHHC
RSGGKIYLEAHTPIDDKGNPSVVDKHTAVINAMLKREDITNNLRMNWDVVRDVVAAEDGL
CCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCC
PTEIGTPNTSAPMVSSAPIDLQQ
CCCCCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MLPRFPAVTRCLSLAALCVAGPVAALEFPLPPPGEDIIGQVQVIKAKYEDTFADLGTTYD
CCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHCEEEEEEEHHHHHHHHCCCEEC
LGYSEMVAANPGVDAWLPGAGTEVVLPTRFILPPGPREGIVINLAEYRLYYYPKGRNVVY
CCCHHEEECCCCCCEECCCCCCEEEEEEEEECCCCCCCCEEEEEEEEEEEEEECCCCEEE
TFPLGIGREGWGSPIAHTTITAKTPNPTWTPPASIKAEHAADGDPLPNVVPAGPDNPLGP
EECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
FKFTLGTPGYLIHGSNKKFGIGMRTSHGCFRMFNNNVLEMASMVPVGTSVRILNDAYKFG
EEEEECCCCEEEECCCCEEEEECCCCCCHHHHHCCCHHHHHHHCCCCCEEEEEHHHHHHC
RSGGKIYLEAHTPIDDKGNPSVVDKHTAVINAMLKREDITNNLRMNWDVVRDVVAAEDGL
CCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCC
PTEIGTPNTSAPMVSSAPIDLQQ
CCCCCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]