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The map label for this gene is kefB [H]

Identifier: 77460188

GI number: 77460188

Start: 4491644

End: 4493356

Strand: Direct

Name: kefB [H]

Synonym: Pfl01_3967

Alternate gene names: 77460188

Gene position: 4491644-4493356 (Clockwise)

Preceding gene: 77460184

Following gene: 77460190

Centisome position: 69.76

GC content: 63.05

Gene sequence:

>1713_bases
GTGTTTGCCAATCTGCTGATCATTCTGGCGTCGTCCCTGGTGATGATTGCCCTGTTCCGACGCCTGCGCCTGCCGCCTGT
GCTGGGTTACCTGTGCGTGGGCTTGATGGTCGGGCCGAAGGCATTCGACTGGATCAATGAAAGCGAACATCTGCCGGATC
TTGCCGAACTGGGCGTGGTGTTTCTGCTGTTTTCCCTGGGGCTGGAGTTCTCCCTGTCGAAAATGATTGCCCTGCGCCAG
GTGGTTTTCCGTCTGGGCAGCCAGCAAGTGATGATCACCACACTCCTGCTGGGAGGCGTGCTTATGTTGCTGGGCATGCC
CGCCACACCAGCCTTGCTGCTGGGCGCCGGCCTCTCGCTATCATCCACGGCGATTGTGACCAAGGAGCTGGGCAGTCTCG
GCGAAATCTTCAGCAGCCACGGCCAGAACGCGGTAGGCGTCCTGTTGTTCCAGGACGTGGTGGCCGTGTTGCTGTTGACG
CTGGTGCCGGTATTTGCCGGGAGCAGTGATCAGGCCTGGTACTGGGCGCTGCCCCTGACCTTGTTGAAGACCGTATTGCT
GTTCTTCGGTTTGTTGCTGGCCAGCCGCTGGCTGCTGCCCCGCCTGTTCCAGGAAGTCGCGGCGTCGCGCTCGGCGGAGC
TGTTCGTGCTGCTGGCCCTGGTGATTGTGTTGCTGACGGCCTGGCTGACCCACCTGCTGGGCCTGTCTCCGGCCCTCGGC
GCGTTCCTCGCGGGCATGCTGCTGGGCGAGAGCCATTACCGGCATCAGATCGAGGCCGATATCCGGCCGTTTCGCGACAT
TCTGCTGGGACTGTTTTTCGTCAGCATCGGCATGCTGATCGACCTGCAGCTGTTCGCCAGCCACAGCCTGCTGATTATCG
GCATGACTCTGGGATTGCTGGTTATCAAAGGTCTGGTGGTGGCGCTGTTGGTGAAATGGCGCGGCAGCGACAGTGAAACG
GCCTGGCGCAGCGGCCTCGCCCTCGCCCAGGGCGGTGAGTTCTGCTTCGCCCTGATGGCGCAGATGCAGCAGACCCGGCT
GCTCCCAGCCGAACTCGGTGCTCTGCTGCTCGCCGCCACCTTCTGCTCGATGCTGCTGACGCCCCTGCTGTTACGCGCCG
CTCCGCGCATCGCCGCCGCCCTGCACCGCAAACCCAATCAGGAAGCACAGATCGAGGAAATCAGTGCCCTCAATGCCGGG
TTCGACGGGCATGTGGTGATCTGTGGTTACGGCCGCGTCGGCCAGTCGATCGGACGCTTCATGCGCAATGCCGGGCAATC
CTACGTCGCGCTGGACAACGATCCGGTGCGAATTCAGGAAGCCGCCACCGCCGAGCGCGACGTCCACTACGGCGATTCAA
CCCGCGGCGATCTGCTGACCGCCGTCGGTTTGCTGCGCGCCAGGTTGCTGGTGATTGCTGTGGATCAAGCCGACGTGGCC
CTGCGTGTTCTCAAGGAGGCTCGACGTCTCAACCCGACAGTGCCGATCCTGGTGCGCACCCGGGACGACAGCCAGTCGGC
CGAGCTGAAAGCAGCCGGCGCCAGCGAAGTGGTGCCGGAACTGCTGGAGTCGAGCCTGATGCTCGGTTCCCATGCCCTGA
TCCTGCTGGGGTTTCCCGCGCACCGGGTGCAGGAGAAAGTCGATCAGGTGCGCATCGATCGTTATCGCCTGCTTCACGGC
TTTTACCCCGGAGCCGACGATGAGGAAACCTAG

Upstream 100 bases:

>100_bases
AAACCCTGATGTAAATCAGCCCACAAAATCGCCATTTACGCGATGCTCCGGTGTTTTTTACCTCTACGCTTGAAGAGCCT
GACCTGTCCCGGAGTGTCTC

Downstream 100 bases:

>100_bases
TCCTGGCTCACCGCGCCGATCTTGTGCAGCGACAGGTCGGCGCCGTAGTACTCCTGTTCCTGGCTCAGGCGCAGACCATG
CAGCGCCTTGATCGAACCGT

Product: Sodium/hydrogen exchanger

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MFANLLIILASSLVMIALFRRLRLPPVLGYLCVGLMVGPKAFDWINESEHLPDLAELGVVFLLFSLGLEFSLSKMIALRQ
VVFRLGSQQVMITTLLLGGVLMLLGMPATPALLLGAGLSLSSTAIVTKELGSLGEIFSSHGQNAVGVLLFQDVVAVLLLT
LVPVFAGSSDQAWYWALPLTLLKTVLLFFGLLLASRWLLPRLFQEVAASRSAELFVLLALVIVLLTAWLTHLLGLSPALG
AFLAGMLLGESHYRHQIEADIRPFRDILLGLFFVSIGMLIDLQLFASHSLLIIGMTLGLLVIKGLVVALLVKWRGSDSET
AWRSGLALAQGGEFCFALMAQMQQTRLLPAELGALLLAATFCSMLLTPLLLRAAPRIAAALHRKPNQEAQIEEISALNAG
FDGHVVICGYGRVGQSIGRFMRNAGQSYVALDNDPVRIQEAATAERDVHYGDSTRGDLLTAVGLLRARLLVIAVDQADVA
LRVLKEARRLNPTVPILVRTRDDSQSAELKAAGASEVVPELLESSLMLGSHALILLGFPAHRVQEKVDQVRIDRYRLLHG
FYPGADDEET

Sequences:

>Translated_570_residues
MFANLLIILASSLVMIALFRRLRLPPVLGYLCVGLMVGPKAFDWINESEHLPDLAELGVVFLLFSLGLEFSLSKMIALRQ
VVFRLGSQQVMITTLLLGGVLMLLGMPATPALLLGAGLSLSSTAIVTKELGSLGEIFSSHGQNAVGVLLFQDVVAVLLLT
LVPVFAGSSDQAWYWALPLTLLKTVLLFFGLLLASRWLLPRLFQEVAASRSAELFVLLALVIVLLTAWLTHLLGLSPALG
AFLAGMLLGESHYRHQIEADIRPFRDILLGLFFVSIGMLIDLQLFASHSLLIIGMTLGLLVIKGLVVALLVKWRGSDSET
AWRSGLALAQGGEFCFALMAQMQQTRLLPAELGALLLAATFCSMLLTPLLLRAAPRIAAALHRKPNQEAQIEEISALNAG
FDGHVVICGYGRVGQSIGRFMRNAGQSYVALDNDPVRIQEAATAERDVHYGDSTRGDLLTAVGLLRARLLVIAVDQADVA
LRVLKEARRLNPTVPILVRTRDDSQSAELKAAGASEVVPELLESSLMLGSHALILLGFPAHRVQEKVDQVRIDRYRLLHG
FYPGADDEET
>Mature_570_residues
MFANLLIILASSLVMIALFRRLRLPPVLGYLCVGLMVGPKAFDWINESEHLPDLAELGVVFLLFSLGLEFSLSKMIALRQ
VVFRLGSQQVMITTLLLGGVLMLLGMPATPALLLGAGLSLSSTAIVTKELGSLGEIFSSHGQNAVGVLLFQDVVAVLLLT
LVPVFAGSSDQAWYWALPLTLLKTVLLFFGLLLASRWLLPRLFQEVAASRSAELFVLLALVIVLLTAWLTHLLGLSPALG
AFLAGMLLGESHYRHQIEADIRPFRDILLGLFFVSIGMLIDLQLFASHSLLIIGMTLGLLVIKGLVVALLVKWRGSDSET
AWRSGLALAQGGEFCFALMAQMQQTRLLPAELGALLLAATFCSMLLTPLLLRAAPRIAAALHRKPNQEAQIEEISALNAG
FDGHVVICGYGRVGQSIGRFMRNAGQSYVALDNDPVRIQEAATAERDVHYGDSTRGDLLTAVGLLRARLLVIAVDQADVA
LRVLKEARRLNPTVPILVRTRDDSQSAELKAAGASEVVPELLESSLMLGSHALILLGFPAHRVQEKVDQVRIDRYRLLHG
FYPGADDEET

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=380, Percent_Identity=28.9473684210526, Blast_Score=100, Evalue=6e-21,
Organism=Escherichia coli, GI1786232, Length=547, Percent_Identity=34.7349177330896, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1789749, Length=562, Percent_Identity=34.8754448398576, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1786685, Length=523, Percent_Identity=31.3575525812619, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6322367, Length=352, Percent_Identity=22.7272727272727, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 61851; Mature: 61851

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFANLLIILASSLVMIALFRRLRLPPVLGYLCVGLMVGPKAFDWINESEHLPDLAELGVV
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHH
FLLFSLGLEFSLSKMIALRQVVFRLGSQQVMITTLLLGGVLMLLGMPATPALLLGAGLSL
HHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
SSTAIVTKELGSLGEIFSSHGQNAVGVLLFQDVVAVLLLTLVPVFAGSSDQAWYWALPLT
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHH
LLKTVLLFFGLLLASRWLLPRLFQEVAASRSAELFVLLALVIVLLTAWLTHLLGLSPALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AFLAGMLLGESHYRHQIEADIRPFRDILLGLFFVSIGMLIDLQLFASHSLLIIGMTLGLL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VIKGLVVALLVKWRGSDSETAWRSGLALAQGGEFCFALMAQMQQTRLLPAELGALLLAAT
HHHHHHHHHHHHHCCCCCHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FCSMLLTPLLLRAAPRIAAALHRKPNQEAQIEEISALNAGFDGHVVICGYGRVGQSIGRF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
MRNAGQSYVALDNDPVRIQEAATAERDVHYGDSTRGDLLTAVGLLRARLLVIAVDQADVA
HHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEECHHHHH
LRVLKEARRLNPTVPILVRTRDDSQSAELKAAGASEVVPELLESSLMLGSHALILLGFPA
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCH
HRVQEKVDQVRIDRYRLLHGFYPGADDEET
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFANLLIILASSLVMIALFRRLRLPPVLGYLCVGLMVGPKAFDWINESEHLPDLAELGVV
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHH
FLLFSLGLEFSLSKMIALRQVVFRLGSQQVMITTLLLGGVLMLLGMPATPALLLGAGLSL
HHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
SSTAIVTKELGSLGEIFSSHGQNAVGVLLFQDVVAVLLLTLVPVFAGSSDQAWYWALPLT
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHH
LLKTVLLFFGLLLASRWLLPRLFQEVAASRSAELFVLLALVIVLLTAWLTHLLGLSPALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AFLAGMLLGESHYRHQIEADIRPFRDILLGLFFVSIGMLIDLQLFASHSLLIIGMTLGLL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VIKGLVVALLVKWRGSDSETAWRSGLALAQGGEFCFALMAQMQQTRLLPAELGALLLAAT
HHHHHHHHHHHHHCCCCCHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
FCSMLLTPLLLRAAPRIAAALHRKPNQEAQIEEISALNAGFDGHVVICGYGRVGQSIGRF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
MRNAGQSYVALDNDPVRIQEAATAERDVHYGDSTRGDLLTAVGLLRARLLVIAVDQADVA
HHHCCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHEEECHHHHH
LRVLKEARRLNPTVPILVRTRDDSQSAELKAAGASEVVPELLESSLMLGSHALILLGFPA
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCH
HRVQEKVDQVRIDRYRLLHGFYPGADDEET
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA