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The map label for this gene is caiD [H]

Identifier: 77460170

GI number: 77460170

Start: 4476661

End: 4477458

Strand: Direct

Name: caiD [H]

Synonym: Pfl01_3949

Alternate gene names: 77460170

Gene position: 4476661-4477458 (Clockwise)

Preceding gene: 77460169

Following gene: 77460171

Centisome position: 69.53

GC content: 65.66

Gene sequence:

>798_bases
ATGAGCGCCCTGCCCGATTGCCAGACGCTGCTGCTGGAACGGCACAACGGCGTGTTGCACATCACCCTCAATCGCCCGGA
AAGCCGCAACGCCATGAGCCTGCAAATGGTCGCCGAGTTGCGCGCCGTGCTGGCCAGCGTGCGCGATGATCGCAGCGTTC
GCGCACTGGTGCTCAGCGGTGCCGGCGGGCATTTCTGTGCCGGCGGCGACATCAAGGACATGGCCAACGCCCGCGCCCAG
GGCAGCGATGCCTATCGCGAATTGAACCGAGCGTTCGGGTTGTTGCTGGAAGAAGCGCAGCACACGCCGCAAGTGCTGAT
CACGGTGTTGCAGGGCGCGGTGCTCGGGGGCGGCTTCGGGCTGGCCTGTGTCAGCGATATCGCCATCGCCGATCATCAGG
CGCAATTCGGTTTGCCGGAGACCAGTCTCGGCCTGCTACCGGCGCAGATCGCGCCGTTTGTGGTGCAGCGCATCGGCCTG
ACCGAGACCCGACGCCTGGCCCTGACCGCCGCCCGTTTCGACGGCCATCAGGCCCGGCGTCTGGGGCTGGTGCATTTTGT
CGAGCAGGATCCACAGGCGCTCGCCGAGCGACTGGATGAAGTGCTCGACCATGTCCTCTGCTGCGCCCCCGAAGCGAATG
CCGTGACGAAAAAACTGCTGCTGGCCAGCGCCGGCCAGCCGTCGAGTGAACTGCTGGACGAAGCCGCGCAGTGGTTCAGC
GAAGCGGTGACCGGTGCCGAAGGCATCGAAGGCACGATGGCGTTTGTGCAAAAGCGCAAACCGAGGTGGGCCCCTTAG

Upstream 100 bases:

>100_bases
CCTACCGCGACGTGCGGCTGGTATCGATTGGCGGCGGCGCCGATGAAATCATGCTGGGGATCATCTGCAAACTGATGGGC
ATTCTGCCGGGGAAAAAGAA

Downstream 100 bases:

>100_bases
AAGCATCGCGGGCAAGCCCGCTCCCACAGTTTTTGTGAGCACCACTGAATCCTGTGGGAGCGGGCTTGCCCGCGATGAGG
CCCTTCAATCCACCGCCAAC

Product: enoyl-CoA hydratase/isomerase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSALPDCQTLLLERHNGVLHITLNRPESRNAMSLQMVAELRAVLASVRDDRSVRALVLSGAGGHFCAGGDIKDMANARAQ
GSDAYRELNRAFGLLLEEAQHTPQVLITVLQGAVLGGGFGLACVSDIAIADHQAQFGLPETSLGLLPAQIAPFVVQRIGL
TETRRLALTAARFDGHQARRLGLVHFVEQDPQALAERLDEVLDHVLCCAPEANAVTKKLLLASAGQPSSELLDEAAQWFS
EAVTGAEGIEGTMAFVQKRKPRWAP

Sequences:

>Translated_265_residues
MSALPDCQTLLLERHNGVLHITLNRPESRNAMSLQMVAELRAVLASVRDDRSVRALVLSGAGGHFCAGGDIKDMANARAQ
GSDAYRELNRAFGLLLEEAQHTPQVLITVLQGAVLGGGFGLACVSDIAIADHQAQFGLPETSLGLLPAQIAPFVVQRIGL
TETRRLALTAARFDGHQARRLGLVHFVEQDPQALAERLDEVLDHVLCCAPEANAVTKKLLLASAGQPSSELLDEAAQWFS
EAVTGAEGIEGTMAFVQKRKPRWAP
>Mature_264_residues
SALPDCQTLLLERHNGVLHITLNRPESRNAMSLQMVAELRAVLASVRDDRSVRALVLSGAGGHFCAGGDIKDMANARAQG
SDAYRELNRAFGLLLEEAQHTPQVLITVLQGAVLGGGFGLACVSDIAIADHQAQFGLPETSLGLLPAQIAPFVVQRIGLT
ETRRLALTAARFDGHQARRLGLVHFVEQDPQALAERLDEVLDHVLCCAPEANAVTKKLLLASAGQPSSELLDEAAQWFSE
AVTGAEGIEGTMAFVQKRKPRWAP

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594469, Length=198, Percent_Identity=32.8282828282828, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI37594471, Length=198, Percent_Identity=32.8282828282828, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=29.8850574712644, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI70995211, Length=233, Percent_Identity=29.6137339055794, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=31.1827956989247, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4502327, Length=261, Percent_Identity=27.2030651340996, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=22.0883534136546, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=27.7153558052434, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=28.6852589641434, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788597, Length=255, Percent_Identity=29.8039215686275, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788682, Length=177, Percent_Identity=32.2033898305085, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=26.9230769230769, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=29.8387096774194, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=202, Percent_Identity=31.6831683168317, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=202, Percent_Identity=31.6831683168317, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=183, Percent_Identity=31.6939890710383, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=282, Percent_Identity=26.241134751773, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=28.6821705426357, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=231, Percent_Identity=27.7056277056277, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=227, Percent_Identity=23.7885462555066, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=240, Percent_Identity=29.5833333333333, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=257, Percent_Identity=26.0700389105058, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=26.9430051813472, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653139, Length=279, Percent_Identity=30.8243727598566, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=29.0836653386454, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=29.0836653386454, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920382, Length=229, Percent_Identity=28.82096069869, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=21.5686274509804, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28571729, Length=254, Percent_Identity=25.5905511811024, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=254, Percent_Identity=25.5905511811024, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650670, Length=193, Percent_Identity=26.4248704663212, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28426; Mature: 28295

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALPDCQTLLLERHNGVLHITLNRPESRNAMSLQMVAELRAVLASVRDDRSVRALVLSG
CCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
AGGHFCAGGDIKDMANARAQGSDAYRELNRAFGLLLEEAQHTPQVLITVLQGAVLGGGFG
CCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHH
LACVSDIAIADHQAQFGLPETSLGLLPAQIAPFVVQRIGLTETRRLALTAARFDGHQARR
HHHHHHHHHHCCHHHCCCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHH
LGLVHFVEQDPQALAERLDEVLDHVLCCAPEANAVTKKLLLASAGQPSSELLDEAAQWFS
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EAVTGAEGIEGTMAFVQKRKPRWAP
HHHCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SALPDCQTLLLERHNGVLHITLNRPESRNAMSLQMVAELRAVLASVRDDRSVRALVLSG
CCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
AGGHFCAGGDIKDMANARAQGSDAYRELNRAFGLLLEEAQHTPQVLITVLQGAVLGGGFG
CCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHH
LACVSDIAIADHQAQFGLPETSLGLLPAQIAPFVVQRIGLTETRRLALTAARFDGHQARR
HHHHHHHHHHCCHHHCCCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHH
LGLVHFVEQDPQALAERLDEVLDHVLCCAPEANAVTKKLLLASAGQPSSELLDEAAQWFS
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EAVTGAEGIEGTMAFVQKRKPRWAP
HHHCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA