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The map label for this gene is flbD [H]

Identifier: 77460139

GI number: 77460139

Start: 4435317

End: 4436420

Strand: Direct

Name: flbD [H]

Synonym: Pfl01_3918

Alternate gene names: 77460139

Gene position: 4435317-4436420 (Clockwise)

Preceding gene: 77460138

Following gene: 77460141

Centisome position: 68.89

GC content: 62.23

Gene sequence:

>1104_bases
ATGCAACTGCTGACCCTGCCGCCTTCACCCGCCCTGGCCACCTCAATCCGCGCCACCGCTCAGGTTTTCGAAGACCCGAA
ATCCCAGGCCCTGCTCGCGCATTTGCAACAGGTCGCCCCGAGCGAGGCCAGCGTGCTGATCATCGGCGAAACCGGCACCG
GCAAAGAGTTGGTGGCGCGGCATATCCATAACCTGAGCAACCGGCGCAACCGGCCGTTCATCGCGGTCAACTGCGGGGCG
TTTTCCGAATCGCTGGTGGAAGCCGAACTGTTCGGCCACGAAAAAGGCGCCTTCACCGGCGCCCTCAGCGCCAAGGCCGG
ATGGTTCGAAGAGGCGGATGGCGGCACCTTGTTCCTCGATGAAATCGGCGATCTGCCGATGGCCATTCAGGTCAAATTGC
TGCGGGTGTTGCAGGAACGCGAAGTGGTGCGCCTCGGATCGCGCAAGAGCATTCCCATCGATGTGCGGGTGCTGGCGGCA
ACCAACGTGCAACTGGAGAAAGCCATCAACGCCGGGCATTTCCGCGAGGATCTGTATTACCGGCTGAACGTAGTGAATCT
GGAGCTGAGCCCGTTGCGCGACCGCCCCGGCGACATCCTGCCGCTGACCCGGCATTTCATCGAGGCCTACAGTCAGCGCC
TGGGTTACGGACGCGTCACCATCAGTCCCGGCGCCGAACACAAGCTGCGCGGCTACAGTTGGCCGGGCAATATCCGCGAA
CTGGAAAACGTCATCCATCACACGCTGCTGATCTGCCGTAACGGCGTGATCGAACGCGACGATCTGCGCCTGTCGAACCT
GCGCATCGACCGTCCCGACGATCAGCACGCAACCGCCGACGACTCACCGGAAGCGCTGCTGGAACGCGCCTTCCAGAAAC
TCTTCGAGCAACAGGCCGGCGCCCTGCATGAAAAAGTCGAAGACGCGTTGCTGCGCTCAGCCTATCGCTTCTGCCATTAC
AACCAGGTGCACACCGCGTCCCTGCTCGGCCTGAGTCGCAACGTCACCCGTACGCGGCTGATCAAGATCGGCGAACTGGC
GGTGAACAAGCGCCGGCCCACGGAAAACCTGCAAGGCGAGCGCTTGATCCAGCTGTCGATCTAA

Upstream 100 bases:

>100_bases
GCTGGATTACAAACTCCACGAGCTGGGCGACTGGGCGCTGAACCAGTCCCTGCCGGTGCCGACGTTCTATTCCTGATTTC
CTTTCGTGGAGCGAGACCCC

Downstream 100 bases:

>100_bases
CCCACCAGTTGGCAGTGCAAGCCGTCGTCATGACTGCGGGCGATGCTGCTCAGCACCTGGAATGAACCGAAGGTCACGGT
GCGCGCCTGATGGGTGCGGA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQLLTLPPSPALATSIRATAQVFEDPKSQALLAHLQQVAPSEASVLIIGETGTGKELVARHIHNLSNRRNRPFIAVNCGA
FSESLVEAELFGHEKGAFTGALSAKAGWFEEADGGTLFLDEIGDLPMAIQVKLLRVLQEREVVRLGSRKSIPIDVRVLAA
TNVQLEKAINAGHFREDLYYRLNVVNLELSPLRDRPGDILPLTRHFIEAYSQRLGYGRVTISPGAEHKLRGYSWPGNIRE
LENVIHHTLLICRNGVIERDDLRLSNLRIDRPDDQHATADDSPEALLERAFQKLFEQQAGALHEKVEDALLRSAYRFCHY
NQVHTASLLGLSRNVTRTRLIKIGELAVNKRRPTENLQGERLIQLSI

Sequences:

>Translated_367_residues
MQLLTLPPSPALATSIRATAQVFEDPKSQALLAHLQQVAPSEASVLIIGETGTGKELVARHIHNLSNRRNRPFIAVNCGA
FSESLVEAELFGHEKGAFTGALSAKAGWFEEADGGTLFLDEIGDLPMAIQVKLLRVLQEREVVRLGSRKSIPIDVRVLAA
TNVQLEKAINAGHFREDLYYRLNVVNLELSPLRDRPGDILPLTRHFIEAYSQRLGYGRVTISPGAEHKLRGYSWPGNIRE
LENVIHHTLLICRNGVIERDDLRLSNLRIDRPDDQHATADDSPEALLERAFQKLFEQQAGALHEKVEDALLRSAYRFCHY
NQVHTASLLGLSRNVTRTRLIKIGELAVNKRRPTENLQGERLIQLSI
>Mature_367_residues
MQLLTLPPSPALATSIRATAQVFEDPKSQALLAHLQQVAPSEASVLIIGETGTGKELVARHIHNLSNRRNRPFIAVNCGA
FSESLVEAELFGHEKGAFTGALSAKAGWFEEADGGTLFLDEIGDLPMAIQVKLLRVLQEREVVRLGSRKSIPIDVRVLAA
TNVQLEKAINAGHFREDLYYRLNVVNLELSPLRDRPGDILPLTRHFIEAYSQRLGYGRVTISPGAEHKLRGYSWPGNIRE
LENVIHHTLLICRNGVIERDDLRLSNLRIDRPDDQHATADDSPEALLERAFQKLFEQQAGALHEKVEDALLRSAYRFCHY
NQVHTASLLGLSRNVTRTRLIKIGELAVNKRRPTENLQGERLIQLSI

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=230, Percent_Identity=55.6521739130435, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1788550, Length=334, Percent_Identity=42.5149700598802, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1790437, Length=257, Percent_Identity=50.5836575875486, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789087, Length=244, Percent_Identity=50, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1789233, Length=313, Percent_Identity=41.5335463258786, Blast_Score=234, Evalue=4e-63,
Organism=Escherichia coli, GI1788905, Length=238, Percent_Identity=51.2605042016807, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=248, Percent_Identity=48.3870967741936, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI1790299, Length=327, Percent_Identity=39.7553516819572, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1786524, Length=241, Percent_Identity=46.8879668049792, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=39.3081761006289, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=41.5966386554622, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87081858, Length=255, Percent_Identity=34.9019607843137, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789828, Length=246, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 41036; Mature: 41036

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLTLPPSPALATSIRATAQVFEDPKSQALLAHLQQVAPSEASVLIIGETGTGKELVAR
CEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
HIHNLSNRRNRPFIAVNCGAFSESLVEAELFGHEKGAFTGALSAKAGWFEEADGGTLFLD
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEHH
EIGDLPMAIQVKLLRVLQEREVVRLGSRKSIPIDVRVLAATNVQLEKAINAGHFREDLYY
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHCCCCHHHCCEE
RLNVVNLELSPLRDRPGDILPLTRHFIEAYSQRLGYGRVTISPGAEHKLRGYSWPGNIRE
EEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHH
LENVIHHTLLICRNGVIERDDLRLSNLRIDRPDDQHATADDSPEALLERAFQKLFEQQAG
HHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALHEKVEDALLRSAYRFCHYNQVHTASLLGLSRNVTRTRLIKIGELAVNKRRPTENLQGE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHEEHHHHHHCCCCCCCCCCCC
RLIQLSI
EEEEEEC
>Mature Secondary Structure
MQLLTLPPSPALATSIRATAQVFEDPKSQALLAHLQQVAPSEASVLIIGETGTGKELVAR
CEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
HIHNLSNRRNRPFIAVNCGAFSESLVEAELFGHEKGAFTGALSAKAGWFEEADGGTLFLD
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEHH
EIGDLPMAIQVKLLRVLQEREVVRLGSRKSIPIDVRVLAATNVQLEKAINAGHFREDLYY
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHCCCCHHHCCEE
RLNVVNLELSPLRDRPGDILPLTRHFIEAYSQRLGYGRVTISPGAEHKLRGYSWPGNIRE
EEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHH
LENVIHHTLLICRNGVIERDDLRLSNLRIDRPDDQHATADDSPEALLERAFQKLFEQQAG
HHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALHEKVEDALLRSAYRFCHYNQVHTASLLGLSRNVTRTRLIKIGELAVNKRRPTENLQGE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHEEHHHHHHCCCCCCCCCCCC
RLIQLSI
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]