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The map label for this gene is citA [H]

Identifier: 77460085

GI number: 77460085

Start: 4365685

End: 4366977

Strand: Direct

Name: citA [H]

Synonym: Pfl01_3864

Alternate gene names: 77460085

Gene position: 4365685-4366977 (Clockwise)

Preceding gene: 77460082

Following gene: 77460087

Centisome position: 67.81

GC content: 62.03

Gene sequence:

>1293_bases
ATGTCCTCAACGACCGGCAAAGGCAAGGCGATTTTTCGCGTTGTCAGCGGCAACTTTCTCGAGATGTTCGACTTCATGGT
CTACGGTTTCTACGCCACGGCCATTGCCAAGACCTTCTTTCCCACCGACAGCGCCTTCGCTTCCCTGATGCTTTCTCTGG
CCACTTTCGGCGCAGGTTTCCTGATGCGTCCGCTGGGGGCGATTTTTCTCGGCGCCTACATAGACCGTCACGGCCGACGC
CAGGGCCTGATCATCACATTGGCCCTGATGGCGGCCGGCACCGTGCTGATTGCCTGCGTGCCGGGCTACGCCACCCTTGG
GGTTGCCGCGCCGCTGATCGTGCTGTTCGGCCGCTTGCTGCAAGGCTTCTCGGCTGGCGTCGAATTAGGCGGTGTGTCGG
TGTACCTGGCGGAAATCGCCACGCCGGGCCGCAAGGGCTTTTTCGTCAGTTGGCAGTCCGCCAGCCAGCAGGCGGCCGTG
GTGTTCGCCGGCCTGCTCGGTGTCGCCCTCAACCATTGGCTGAGCCCTGAGCAGATGGGCGAATGGGGCTGGCGGGTGCC
GTTCCTGATCGGCTGCATGATCGTCCCGGTGATTTTCGTCATCCGCCGCTCGCTGGAGGAAACCCCGGAGTTCCAGGCGC
GCAAACATCGCCCTACCCTGCAGGAAATTGTCCGCTCGATCGGTCAGAACTTTGGCATCGTCATCGCCGGCATGGCGCTG
GTGGTCATGACCACGGTGTCGTTCTACCTGATCACGGCCTACACACCGACGTTCGGCAAGGCGGAATTGCACCTGTCGGA
CTTCGATGCGTTGCTGGTGACCGTGTGCATCGGTCTGTCGAACTTCTTCTGGTTGCCGGTGATGGGCGCGGTGTCTGACA
AGGTCGGGCGTAAACCCCTACTGCTGGCGGCGACGATTCTGGCGATCCTGACCGCTTACCCGGCGCTGTCGTGGCTGGTG
GCGAACCCGAGCTTCAGCCATTTGCTGATCGTCGAGCTGTGGCTGTCGTTCCTGTACGGCTCGTACAACGGGGCAATGGT
GGTGGCACTCACCGAAATCATGCCGGTAGAAGTTCGTACGACTGGCTTCTCGCTGGCCTACAGCCTGGCGACCGCCACCT
TTGGTGGCTTTACCCCGGCGGCCTGCACTTATCTGATCCATGTGCTGGACAACAAGGCGGCACCGGGGATCTGGCTCAGT
GGTGCAGCGGTATTGGGCGTGATCGCCACGCTGGTGCTGTTTCGTGGCAACAGGCACGAACTGCGCACGGCACAAGCCGC
CATCCCCCGCTAA

Upstream 100 bases:

>100_bases
CGTCCAGTTAACAATTCTTCATCTCCCCGCAAAACCGCGCCGGCCATTATGGGCTACGAGCGCAACGCTTTCTTACAAGA
TTTAGAGTGAGTACCTACCC

Downstream 100 bases:

>100_bases
GGCAAATCGCGCACAAACAAAAACGCCCCGACCAGAGTCGGGGCGTTTTCATTTGCAGCTAAGGCTTAGCGCGGGAACGC
TGGCGGGTTTACACCGGCCA

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSSTTGKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPTDSAFASLMLSLATFGAGFLMRPLGAIFLGAYIDRHGRR
QGLIITLALMAAGTVLIACVPGYATLGVAAPLIVLFGRLLQGFSAGVELGGVSVYLAEIATPGRKGFFVSWQSASQQAAV
VFAGLLGVALNHWLSPEQMGEWGWRVPFLIGCMIVPVIFVIRRSLEETPEFQARKHRPTLQEIVRSIGQNFGIVIAGMAL
VVMTTVSFYLITAYTPTFGKAELHLSDFDALLVTVCIGLSNFFWLPVMGAVSDKVGRKPLLLAATILAILTAYPALSWLV
ANPSFSHLLIVELWLSFLYGSYNGAMVVALTEIMPVEVRTTGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLS
GAAVLGVIATLVLFRGNRHELRTAQAAIPR

Sequences:

>Translated_430_residues
MSSTTGKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPTDSAFASLMLSLATFGAGFLMRPLGAIFLGAYIDRHGRR
QGLIITLALMAAGTVLIACVPGYATLGVAAPLIVLFGRLLQGFSAGVELGGVSVYLAEIATPGRKGFFVSWQSASQQAAV
VFAGLLGVALNHWLSPEQMGEWGWRVPFLIGCMIVPVIFVIRRSLEETPEFQARKHRPTLQEIVRSIGQNFGIVIAGMAL
VVMTTVSFYLITAYTPTFGKAELHLSDFDALLVTVCIGLSNFFWLPVMGAVSDKVGRKPLLLAATILAILTAYPALSWLV
ANPSFSHLLIVELWLSFLYGSYNGAMVVALTEIMPVEVRTTGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLS
GAAVLGVIATLVLFRGNRHELRTAQAAIPR
>Mature_429_residues
SSTTGKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPTDSAFASLMLSLATFGAGFLMRPLGAIFLGAYIDRHGRRQ
GLIITLALMAAGTVLIACVPGYATLGVAAPLIVLFGRLLQGFSAGVELGGVSVYLAEIATPGRKGFFVSWQSASQQAAVV
FAGLLGVALNHWLSPEQMGEWGWRVPFLIGCMIVPVIFVIRRSLEETPEFQARKHRPTLQEIVRSIGQNFGIVIAGMALV
VMTTVSFYLITAYTPTFGKAELHLSDFDALLVTVCIGLSNFFWLPVMGAVSDKVGRKPLLLAATILAILTAYPALSWLVA
NPSFSHLLIVELWLSFLYGSYNGAMVVALTEIMPVEVRTTGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLSG
AAVLGVIATLVLFRGNRHELRTAQAAIPR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=435, Percent_Identity=30.8045977011494, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788942, Length=384, Percent_Identity=34.6354166666667, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1788292, Length=421, Percent_Identity=26.8408551068884, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789941, Length=431, Percent_Identity=30.3944315545244, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082404, Length=387, Percent_Identity=25.0645994832041, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082231, Length=208, Percent_Identity=29.8076923076923, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46229; Mature: 46098

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTTGKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPTDSAFASLMLSLATFGAGF
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHH
LMRPLGAIFLGAYIDRHGRRQGLIITLALMAAGTVLIACVPGYATLGVAAPLIVLFGRLL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHH
QGFSAGVELGGVSVYLAEIATPGRKGFFVSWQSASQQAAVVFAGLLGVALNHWLSPEQMG
HHHHCCCCCCCHHEEEEHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHC
EWGWRVPFLIGCMIVPVIFVIRRSLEETPEFQARKHRPTLQEIVRSIGQNFGIVIAGMAL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHH
VVMTTVSFYLITAYTPTFGKAELHLSDFDALLVTVCIGLSNFFWLPVMGAVSDKVGRKPL
HHHHHHHHHHHEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LLAATILAILTAYPALSWLVANPSFSHLLIVELWLSFLYGSYNGAMVVALTEIMPVEVRT
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHCEEEEEE
TGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLSGAAVLGVIATLVLFRGNRHE
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHCCCCHH
LRTAQAAIPR
HHHHHHCCCC
>Mature Secondary Structure 
SSTTGKGKAIFRVVSGNFLEMFDFMVYGFYATAIAKTFFPTDSAFASLMLSLATFGAGF
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHH
LMRPLGAIFLGAYIDRHGRRQGLIITLALMAAGTVLIACVPGYATLGVAAPLIVLFGRLL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHH
QGFSAGVELGGVSVYLAEIATPGRKGFFVSWQSASQQAAVVFAGLLGVALNHWLSPEQMG
HHHHCCCCCCCHHEEEEHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHC
EWGWRVPFLIGCMIVPVIFVIRRSLEETPEFQARKHRPTLQEIVRSIGQNFGIVIAGMAL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHH
VVMTTVSFYLITAYTPTFGKAELHLSDFDALLVTVCIGLSNFFWLPVMGAVSDKVGRKPL
HHHHHHHHHHHEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
LLAATILAILTAYPALSWLVANPSFSHLLIVELWLSFLYGSYNGAMVVALTEIMPVEVRT
HHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHCEEEEEE
TGFSLAYSLATATFGGFTPAACTYLIHVLDNKAAPGIWLSGAAVLGVIATLVLFRGNRHE
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHCCCCHH
LRTAQAAIPR
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]