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The map label for this gene is bkdA1 [H]

Identifier: 77459685

GI number: 77459685

Start: 3947327

End: 3948562

Strand: Direct

Name: bkdA1 [H]

Synonym: Pfl01_3463

Alternate gene names: 77459685

Gene position: 3947327-3948562 (Clockwise)

Preceding gene: 77459683

Following gene: 77459686

Centisome position: 61.31

GC content: 63.27

Gene sequence:

>1236_bases
ATGACCCAAGCGTATGAACCGCTGCGTCTGCACGTCCCTGAACCCTCGGGCCGCCCAGGCTGCAAAACCGATTTCTCCTA
CCTGCATCTGACCGATGCCGGCACGGTGCGCAAACCTTCCATCGACGTAGAACCCGCCGACACCGCCGACCTGGCCCGTG
GCCTGATCCGCGTGCTCGACGACCAGGGCAACGCCCTCGGCCCATGGGCTGAAAACGTTCCGGTCGAGATCCTGCGCAAA
GGCATGCGCGCCATGCTCAAGACCCGGATCTACGACAACCGCATGGTGGTTGCCCAGCGTCAGAAAAAAATGTCGTTCTA
CATGCAGAGCCTTGGCGAAGAAGCCATTGGCAGTGCCCAGGCCCTGGCCTTGAACATCGACGACATGTGCTTCCCCACCT
ACCGCCAGCAAAGCATTCTGATGGCCCGCGACGTACCGCTGGTCGACCTGATCTGCCAGTTGCTGTCCAACGAGCGCGAT
CCACTCAAGGGACGTCAGCTGCCGATCATGTACTCGGTCAAGGACGCCGGTTTCTTCACCATTTCCGGTAACCTCGCCAC
CCAGTTCATTCAGGGCGTGGGCTGGGGCATGGCCTCGGCGATCAAAGGCGACACCAAAATCGCCTCGGCCTGGATCGGCG
ACGGCGCCACCGCCGAATCGGACTTCCACACCGCCCTCACCTTCGCTCACGTCTATCGTGCGCCGGTGATCCTCAACGTG
GTCAACAACCAGTGGGCGATCTCGACCTTCCAGGCCATCGCCGGTGGTGAAGCCACCACCTTCGCCGGACGCGGTGTCGG
TTGCGGCATCGCCTCGCTGCGGGTGGATGGCAACGATTTTTACGCGGTCTACGCCGCCTCCGCCTGGGCTGCCGAACGCG
CCCGCCGCAACCTCGGCCCGACCATGATCGAATGGGTCACCTACCGCGCCGGCCCGCACTCGACCTCCGACGATCCGTCC
AAATACCGTCCTGCCGACGACTGGAGCCACTTCCCGCTGGGCGACCCGATTGCCCGCCTGAAACAGCACCTGATCAAAGT
CGGCCACTGGTCGGAAGAAGAGCACGCCGCCGTCAGCGCCGAGCTCGAAGCCGAAGTGATCGCCGCACAGAAACAGGCCG
AACAGTACGGCACCCTCGCCGGCGGCCAGATTCCGAGCGCCGCCACCATGTTCGAAGACGTCTACAAAGAGATGCCGGAG
CACTTGAAGCGCCAGCGTCAGCAGTTGGGGATCTGA

Upstream 100 bases:

>100_bases
TTCTTGCCCGGGCCTATGATTGTTGTAGGACAAGATTTCTTTTATCGAAGAATGACTGCCAGCTCACTACAAGAAATTCA
CAAGATCGAGCGTAGAAGCC

Downstream 100 bases:

>100_bases
CATGAACGATCACAACAACAATATTCAGTTGGAAACCGCCATGACCACGACCACCATGACCATGATCCAGGCCCTGCGCT
CGGCCATGGATGTGATGCTT

Product: branched-chain alpha-keto acid dehydrogenase E1 component

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTQAYEPLRLHVPEPSGRPGCKTDFSYLHLTDAGTVRKPSIDVEPADTADLARGLIRVLDDQGNALGPWAENVPVEILRK
GMRAMLKTRIYDNRMVVAQRQKKMSFYMQSLGEEAIGSAQALALNIDDMCFPTYRQQSILMARDVPLVDLICQLLSNERD
PLKGRQLPIMYSVKDAGFFTISGNLATQFIQGVGWGMASAIKGDTKIASAWIGDGATAESDFHTALTFAHVYRAPVILNV
VNNQWAISTFQAIAGGEATTFAGRGVGCGIASLRVDGNDFYAVYAASAWAAERARRNLGPTMIEWVTYRAGPHSTSDDPS
KYRPADDWSHFPLGDPIARLKQHLIKVGHWSEEEHAAVSAELEAEVIAAQKQAEQYGTLAGGQIPSAATMFEDVYKEMPE
HLKRQRQQLGI

Sequences:

>Translated_411_residues
MTQAYEPLRLHVPEPSGRPGCKTDFSYLHLTDAGTVRKPSIDVEPADTADLARGLIRVLDDQGNALGPWAENVPVEILRK
GMRAMLKTRIYDNRMVVAQRQKKMSFYMQSLGEEAIGSAQALALNIDDMCFPTYRQQSILMARDVPLVDLICQLLSNERD
PLKGRQLPIMYSVKDAGFFTISGNLATQFIQGVGWGMASAIKGDTKIASAWIGDGATAESDFHTALTFAHVYRAPVILNV
VNNQWAISTFQAIAGGEATTFAGRGVGCGIASLRVDGNDFYAVYAASAWAAERARRNLGPTMIEWVTYRAGPHSTSDDPS
KYRPADDWSHFPLGDPIARLKQHLIKVGHWSEEEHAAVSAELEAEVIAAQKQAEQYGTLAGGQIPSAATMFEDVYKEMPE
HLKRQRQQLGI
>Mature_410_residues
TQAYEPLRLHVPEPSGRPGCKTDFSYLHLTDAGTVRKPSIDVEPADTADLARGLIRVLDDQGNALGPWAENVPVEILRKG
MRAMLKTRIYDNRMVVAQRQKKMSFYMQSLGEEAIGSAQALALNIDDMCFPTYRQQSILMARDVPLVDLICQLLSNERDP
LKGRQLPIMYSVKDAGFFTISGNLATQFIQGVGWGMASAIKGDTKIASAWIGDGATAESDFHTALTFAHVYRAPVILNVV
NNQWAISTFQAIAGGEATTFAGRGVGCGIASLRVDGNDFYAVYAASAWAAERARRNLGPTMIEWVTYRAGPHSTSDDPSK
YRPADDWSHFPLGDPIARLKQHLIKVGHWSEEEHAAVSAELEAEVIAAQKQAEQYGTLAGGQIPSAATMFEDVYKEMPEH
LKRQRQQLGI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=357, Percent_Identity=38.0952380952381, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI258645172, Length=361, Percent_Identity=37.6731301939058, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4885543, Length=289, Percent_Identity=25.2595155709343, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4505685, Length=268, Percent_Identity=26.4925373134328, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI291084742, Length=268, Percent_Identity=26.4925373134328, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI291084744, Length=273, Percent_Identity=26.007326007326, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI86563357, Length=333, Percent_Identity=37.2372372372372, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI86563355, Length=333, Percent_Identity=37.2372372372372, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI32564172, Length=302, Percent_Identity=26.158940397351, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17536047, Length=302, Percent_Identity=26.158940397351, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321026, Length=339, Percent_Identity=28.023598820059, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21355903, Length=373, Percent_Identity=34.0482573726542, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24639748, Length=292, Percent_Identity=26.7123287671233, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24639740, Length=190, Percent_Identity=29.4736842105263, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24639744, Length=190, Percent_Identity=29.4736842105263, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28571106, Length=190, Percent_Identity=29.4736842105263, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24639746, Length=291, Percent_Identity=25.7731958762887, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 45169; Mature: 45038

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAYEPLRLHVPEPSGRPGCKTDFSYLHLTDAGTVRKPSIDVEPADTADLARGLIRVLD
CCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
DQGNALGPWAENVPVEILRKGMRAMLKTRIYDNRMVVAQRQKKMSFYMQSLGEEAIGSAQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCHHHCCCCE
ALALNIDDMCFPTYRQQSILMARDVPLVDLICQLLSNERDPLKGRQLPIMYSVKDAGFFT
EEEECCCCCCCCCHHCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEE
ISGNLATQFIQGVGWGMASAIKGDTKIASAWIGDGATAESDFHTALTFAHVYRAPVILNV
EECHHHHHHHHHHCCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEE
VNNQWAISTFQAIAGGEATTFAGRGVGCGIASLRVDGNDFYAVYAASAWAAERARRNLGP
CCCCCHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHCCCC
TMIEWVTYRAGPHSTSDDPSKYRPADDWSHFPLGDPIARLKQHLIKVGHWSEEEHAAVSA
HHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
ELEAEVIAAQKQAEQYGTLAGGQIPSAATMFEDVYKEMPEHLKRQRQQLGI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQAYEPLRLHVPEPSGRPGCKTDFSYLHLTDAGTVRKPSIDVEPADTADLARGLIRVLD
CCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
DQGNALGPWAENVPVEILRKGMRAMLKTRIYDNRMVVAQRQKKMSFYMQSLGEEAIGSAQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCHHHCCCCE
ALALNIDDMCFPTYRQQSILMARDVPLVDLICQLLSNERDPLKGRQLPIMYSVKDAGFFT
EEEECCCCCCCCCHHCCCCCEECCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEE
ISGNLATQFIQGVGWGMASAIKGDTKIASAWIGDGATAESDFHTALTFAHVYRAPVILNV
EECHHHHHHHHHHCCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEE
VNNQWAISTFQAIAGGEATTFAGRGVGCGIASLRVDGNDFYAVYAASAWAAERARRNLGP
CCCCCHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHCCCC
TMIEWVTYRAGPHSTSDDPSKYRPADDWSHFPLGDPIARLKQHLIKVGHWSEEEHAAVSA
HHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
ELEAEVIAAQKQAEQYGTLAGGQIPSAATMFEDVYKEMPEHLKRQRQQLGI
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3416875; 2211503; 8521848; 10426958 [H]