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The map label for this gene is ycbB [H]

Identifier: 77459598

GI number: 77459598

Start: 3850188

End: 3851801

Strand: Reverse

Name: ycbB [H]

Synonym: Pfl01_3376

Alternate gene names: 77459598

Gene position: 3851801-3850188 (Counterclockwise)

Preceding gene: 77459603

Following gene: 77459595

Centisome position: 59.83

GC content: 65.24

Gene sequence:

>1614_bases
TTGTTCAAAAAGTACGCATGCTACTTGAGCATTTGTTTGCTCGCTGCGCCGTTTGTCGCTTGCGCCGAAGAGCCGTTGCC
GGGACTCCCGACGCTGCCGCCCGTGCCGACGGAAATGCCCATCGAACCGCAGAGCCCGTTGCAGGCTGCGCTGATCAGCC
TGCCCCAGGCGTGCCCGACGATCGGCACCAGCCTCAGCGGCCCGGCGCTGGATCAGTTACGCGCGTTCTACCAGCAGCAG
GACTGGCTGCCGGTGTGGGCCGATGGCGGGCAATTGCCGGCCTTGCATGCGCAATTGCAGCTGTTGGCCGACGATGGCCT
GAACCCGAAACGCTATCCGGTGGCGCTCACCGCCCCGCAGGACGGCGAACTGTGCGCCGATATCGAGATCAGCCGTTACT
ACCTGCAAGCCCTGCAGGATCTGCATTACGGGCGCCTGCTGCAATCGCACTTCGAACCGCTGTGGCATTCCGACGAAACG
CCACGCGACCGGCAGGCCGAATTGCTCGCCATCGCCGTACCCGGCGTGCACAACGTTCCGGCCGCCTTCGATCTGGCCCG
CCCGCAGCTGGCGCAATATCAGAATCTGCGTCATCTGTATGCCGCGCAGCGCCTGAAAGCCTTGCCGCAGTGGACGTCGG
TGGGCAACGGTCCTTTGCTGCGTCCGGACATGGAAGACAAACGCGTGCCGGAACTGGCCCGCCGGCTGTACAGCGAAGGC
TATCTGAACAGCGTGGCCGGCACGCCGGACAACGCTTATCACGGCGTATTGGTGGAGGCGGTGAAGAGCTTTCAGGCCAA
CCATTCGTTGCAGGCCGACGGGGTGGTGGGGCCGGGGACGATTGCCGAACTCAACATCAGTCCGCTGACTCGCCGCGAAC
AGTTGCGGGTCAACCTTGAACGTTTCCGCTGGATGGCCCAGGACATGGAGCCGGACGGTCTGCTGGTCAACGTCGCCGCG
GCCGAGCTGACGCTTTACCAGGGTGGCCTGCCGGTGTGGCAGACCCGCACCCAGGTCGGGCGCGCTGAACGCCAGACCCC
GCTGCTCAAATCCCGGGTCACGCGCCTGACCCTGAACCCGACCTGGACCGTGCCGCCGACCATCTGGAAGGAAGACAAAC
TGCCGGAAATCCGCAAGGACCAGACGTTCCTCAGCCGCCAGAACCTGCAGGTGCTCGACGCCAACGGCCAGCCGCTGGCC
GCCGCCGACATCGACTGGGACAACCCCGGCAACATCCTCCTGCGCCAGGACGCCGGGCCGCGCAACCCGCTGGGGCAAAT
GGTCATTCGTTTCCCCAACCCGTTCTCGGTGTACCTGCACGACACGCCGAGCAAAGCGCTGTTCGACAAGGGACCACGGG
CGTTCAGCTCCGGCTGCGTACGGGTCGAGCATCCGATGAAGCTGCGCGATTTGCTGCTCAGCCCGGCGGAGAAGGCGCGC
ACCGACACCTTGCTCGCCACCGGCGTCACCCATGAATTCCGCCTGAGCAATCCCGTGCCGATCCTCATGACTTACTGGAC
GGCCCAGGTTGACGGCCAGGGCCGCGTGCTTTACGCGCCGGACATCTACAGCCGGGACAGTGCGTTGCTGGCGGGGCTGG
ACCGGGCGCATTGA

Upstream 100 bases:

>100_bases
CAGTCGTCCCCGGCAGGCCCGCGAATCGGGCCTGACTGTTCACCGCACCTGAGGTAAGGTTGACCGTCAATCAGGACGGC
CATGGATTGGAGTAAAGCAG

Downstream 100 bases:

>100_bases
TCCTCAGCGGCTGGCGCGTTTCCAGAAAAACCGCTCCAGCCACGCATCCACACTCAACTTGCCGGCACCGGCGAATACCA
GCGGCACCAGTGCGGCGAGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MFKKYACYLSICLLAAPFVACAEEPLPGLPTLPPVPTEMPIEPQSPLQAALISLPQACPTIGTSLSGPALDQLRAFYQQQ
DWLPVWADGGQLPALHAQLQLLADDGLNPKRYPVALTAPQDGELCADIEISRYYLQALQDLHYGRLLQSHFEPLWHSDET
PRDRQAELLAIAVPGVHNVPAAFDLARPQLAQYQNLRHLYAAQRLKALPQWTSVGNGPLLRPDMEDKRVPELARRLYSEG
YLNSVAGTPDNAYHGVLVEAVKSFQANHSLQADGVVGPGTIAELNISPLTRREQLRVNLERFRWMAQDMEPDGLLVNVAA
AELTLYQGGLPVWQTRTQVGRAERQTPLLKSRVTRLTLNPTWTVPPTIWKEDKLPEIRKDQTFLSRQNLQVLDANGQPLA
AADIDWDNPGNILLRQDAGPRNPLGQMVIRFPNPFSVYLHDTPSKALFDKGPRAFSSGCVRVEHPMKLRDLLLSPAEKAR
TDTLLATGVTHEFRLSNPVPILMTYWTAQVDGQGRVLYAPDIYSRDSALLAGLDRAH

Sequences:

>Translated_537_residues
MFKKYACYLSICLLAAPFVACAEEPLPGLPTLPPVPTEMPIEPQSPLQAALISLPQACPTIGTSLSGPALDQLRAFYQQQ
DWLPVWADGGQLPALHAQLQLLADDGLNPKRYPVALTAPQDGELCADIEISRYYLQALQDLHYGRLLQSHFEPLWHSDET
PRDRQAELLAIAVPGVHNVPAAFDLARPQLAQYQNLRHLYAAQRLKALPQWTSVGNGPLLRPDMEDKRVPELARRLYSEG
YLNSVAGTPDNAYHGVLVEAVKSFQANHSLQADGVVGPGTIAELNISPLTRREQLRVNLERFRWMAQDMEPDGLLVNVAA
AELTLYQGGLPVWQTRTQVGRAERQTPLLKSRVTRLTLNPTWTVPPTIWKEDKLPEIRKDQTFLSRQNLQVLDANGQPLA
AADIDWDNPGNILLRQDAGPRNPLGQMVIRFPNPFSVYLHDTPSKALFDKGPRAFSSGCVRVEHPMKLRDLLLSPAEKAR
TDTLLATGVTHEFRLSNPVPILMTYWTAQVDGQGRVLYAPDIYSRDSALLAGLDRAH
>Mature_537_residues
MFKKYACYLSICLLAAPFVACAEEPLPGLPTLPPVPTEMPIEPQSPLQAALISLPQACPTIGTSLSGPALDQLRAFYQQQ
DWLPVWADGGQLPALHAQLQLLADDGLNPKRYPVALTAPQDGELCADIEISRYYLQALQDLHYGRLLQSHFEPLWHSDET
PRDRQAELLAIAVPGVHNVPAAFDLARPQLAQYQNLRHLYAAQRLKALPQWTSVGNGPLLRPDMEDKRVPELARRLYSEG
YLNSVAGTPDNAYHGVLVEAVKSFQANHSLQADGVVGPGTIAELNISPLTRREQLRVNLERFRWMAQDMEPDGLLVNVAA
AELTLYQGGLPVWQTRTQVGRAERQTPLLKSRVTRLTLNPTWTVPPTIWKEDKLPEIRKDQTFLSRQNLQVLDANGQPLA
AADIDWDNPGNILLRQDAGPRNPLGQMVIRFPNPFSVYLHDTPSKALFDKGPRAFSSGCVRVEHPMKLRDLLLSPAEKAR
TDTLLATGVTHEFRLSNPVPILMTYWTAQVDGQGRVLYAPDIYSRDSALLAGLDRAH

Specific function: Responsible, at least in part, for generating a meso- diaminopimelyl-3-a meso-diaminopimelyl-3 cross-link [H]

COG id: COG2989

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787155, Length=283, Percent_Identity=37.4558303886926, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002477
- InterPro:   IPR005490 [H]

Pfam domain/function: PF01471 PG_binding_1; PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 59698; Mature: 59698

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKYACYLSICLLAAPFVACAEEPLPGLPTLPPVPTEMPIEPQSPLQAALISLPQACPT
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHH
IGTSLSGPALDQLRAFYQQQDWLPVWADGGQLPALHAQLQLLADDGLNPKRYPVALTAPQ
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCC
DGELCADIEISRYYLQALQDLHYGRLLQSHFEPLWHSDETPRDRQAELLAIAVPGVHNVP
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCC
AAFDLARPQLAQYQNLRHLYAAQRLKALPQWTSVGNGPLLRPDMEDKRVPELARRLYSEG
CHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
YLNSVAGTPDNAYHGVLVEAVKSFQANHSLQADGVVGPGTIAELNISPLTRREQLRVNLE
CHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
RFRWMAQDMEPDGLLVNVAAAELTLYQGGLPVWQTRTQVGRAERQTPLLKSRVTRLTLNP
HHHHHHHCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHCEEEEECC
TWTVPPTIWKEDKLPEIRKDQTFLSRQNLQVLDANGQPLAAADIDWDNPGNILLRQDAGP
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCEEEECCCCC
RNPLGQMVIRFPNPFSVYLHDTPSKALFDKGPRAFSSGCVRVEHPMKLRDLLLSPAEKAR
CCCHHHEEEECCCCEEEEEECCCCHHHHCCCCHHHHCCCEEECCCHHHHHHHHCCHHHHH
TDTLLATGVTHEFRLSNPVPILMTYWTAQVDGQGRVLYAPDIYSRDSALLAGLDRAH
CCCEEEECCCEEEECCCCCCEEEEEEEEEECCCCCEEECCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MFKKYACYLSICLLAAPFVACAEEPLPGLPTLPPVPTEMPIEPQSPLQAALISLPQACPT
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHH
IGTSLSGPALDQLRAFYQQQDWLPVWADGGQLPALHAQLQLLADDGLNPKRYPVALTAPQ
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCC
DGELCADIEISRYYLQALQDLHYGRLLQSHFEPLWHSDETPRDRQAELLAIAVPGVHNVP
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCC
AAFDLARPQLAQYQNLRHLYAAQRLKALPQWTSVGNGPLLRPDMEDKRVPELARRLYSEG
CHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
YLNSVAGTPDNAYHGVLVEAVKSFQANHSLQADGVVGPGTIAELNISPLTRREQLRVNLE
CHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
RFRWMAQDMEPDGLLVNVAAAELTLYQGGLPVWQTRTQVGRAERQTPLLKSRVTRLTLNP
HHHHHHHCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHCEEEEECC
TWTVPPTIWKEDKLPEIRKDQTFLSRQNLQVLDANGQPLAAADIDWDNPGNILLRQDAGP
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCEEEECCCCC
RNPLGQMVIRFPNPFSVYLHDTPSKALFDKGPRAFSSGCVRVEHPMKLRDLLLSPAEKAR
CCCHHHEEEECCCCEEEEEECCCCHHHHCCCCHHHHCCCEEECCCHHHHHHHHCCHHHHH
TDTLLATGVTHEFRLSNPVPILMTYWTAQVDGQGRVLYAPDIYSRDSALLAGLDRAH
CCCEEEECCCEEEECCCCCCEEEEEEEEEECCCCCEEECCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1989883 [H]