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The map label for this gene is cynT [H]

Identifier: 77459593

GI number: 77459593

Start: 3845977

End: 3846636

Strand: Direct

Name: cynT [H]

Synonym: Pfl01_3371

Alternate gene names: 77459593

Gene position: 3845977-3846636 (Clockwise)

Preceding gene: 77459592

Following gene: 77459596

Centisome position: 59.73

GC content: 62.42

Gene sequence:

>660_bases
ATGAAAAACCTGATCGAAGGCTTCCTGAAGTTCCAGAACGAAGCGTTTCCACAACGCACCGAGCTGTTCAAACACCTGGC
GACGACTCAAACCCCGGGCACCTTGTTCATCACCTGCTCCGACAGCCGCGTCGTCCCGGAACTGCTGACCCAGCAAGAGC
CCGGCGAGCTGTTCGTGATCCGTAACGCCGGCAATATCGTGCCGTCCTACAGCCCGCATCCGGGCGGGGTCTCGGCAACG
GTGGAATACGCGGTAGCCGTGCTCGGCGTGACCGACATCGTGATCTGCGGCCACTCCGATTGCGGCGCGATGACCGCCAT
CGCCCAGTGCAAATGCATGGATCACCTGCCCGCCGTCAGCGGCTGGCTGCAACACGCCGAGTCGGCGAAAGTGGTCAACG
AATCGCGGCCACACGCCAATGACGCCGCCAAACTGAGTTCGATGGTGCGGGAAAACGTGATCGCGCAACTGGCGAACATC
CAGACTCACCCGAGCGTGCGCCTGGCCCAGGAGAAAGGCCTGCTGAATCTGCATGGCTGGGTGTACGACATCGAGACCGG
CTCGATCGACGCGCTGAGTGCCGACCGTCGCACTTTCGTCTCGCTGGCCGAGCAACCGTCGACGTGCGCGGTGTACGGTC
AGACAGTCGATGCGGCTTGA

Upstream 100 bases:

>100_bases
AGTGCCGGTCGAGATGACTTACCTCGACAGCCACGGCGCAAAACACATTCTTCAATACAGCGAATTCGGCACCGGCTGTT
CGAACTGAAAGGACTACACC

Downstream 100 bases:

>100_bases
AAACACAACGCCGCGCCCGTCATTGCGACGGGCGCGGCGTTGTTCTGTTCAGCGTTTTACGCCCAGATCGATTTGCGTCA
TACGGCTGCGGACGGTGAAC

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase 1 [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKNLIEGFLKFQNEAFPQRTELFKHLATTQTPGTLFITCSDSRVVPELLTQQEPGELFVIRNAGNIVPSYSPHPGGVSAT
VEYAVAVLGVTDIVICGHSDCGAMTAIAQCKCMDHLPAVSGWLQHAESAKVVNESRPHANDAAKLSSMVRENVIAQLANI
QTHPSVRLAQEKGLLNLHGWVYDIETGSIDALSADRRTFVSLAEQPSTCAVYGQTVDAA

Sequences:

>Translated_219_residues
MKNLIEGFLKFQNEAFPQRTELFKHLATTQTPGTLFITCSDSRVVPELLTQQEPGELFVIRNAGNIVPSYSPHPGGVSAT
VEYAVAVLGVTDIVICGHSDCGAMTAIAQCKCMDHLPAVSGWLQHAESAKVVNESRPHANDAAKLSSMVRENVIAQLANI
QTHPSVRLAQEKGLLNLHGWVYDIETGSIDALSADRRTFVSLAEQPSTCAVYGQTVDAA
>Mature_219_residues
MKNLIEGFLKFQNEAFPQRTELFKHLATTQTPGTLFITCSDSRVVPELLTQQEPGELFVIRNAGNIVPSYSPHPGGVSAT
VEYAVAVLGVTDIVICGHSDCGAMTAIAQCKCMDHLPAVSGWLQHAESAKVVNESRPHANDAAKLSSMVRENVIAQLANI
QTHPSVRLAQEKGLLNLHGWVYDIETGSIDALSADRRTFVSLAEQPSTCAVYGQTVDAA

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=211, Percent_Identity=71.563981042654, Blast_Score=328, Evalue=1e-91,
Organism=Escherichia coli, GI1786318, Length=183, Percent_Identity=34.4262295081967, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25147564, Length=245, Percent_Identity=29.7959183673469, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI115532990, Length=244, Percent_Identity=26.2295081967213, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324292, Length=182, Percent_Identity=31.3186813186813, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24645213, Length=248, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23600; Mature: 23600

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLIEGFLKFQNEAFPQRTELFKHLATTQTPGTLFITCSDSRVVPELLTQQEPGELFVI
CHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEE
RNAGNIVPSYSPHPGGVSATVEYAVAVLGVTDIVICGHSDCGAMTAIAQCKCMDHLPAVS
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCHHHH
GWLQHAESAKVVNESRPHANDAAKLSSMVRENVIAQLANIQTHPSVRLAQEKGLLNLHGW
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCEEECCE
VYDIETGSIDALSADRRTFVSLAEQPSTCAVYGQTVDAA
EEEECCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKNLIEGFLKFQNEAFPQRTELFKHLATTQTPGTLFITCSDSRVVPELLTQQEPGELFVI
CHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEE
RNAGNIVPSYSPHPGGVSATVEYAVAVLGVTDIVICGHSDCGAMTAIAQCKCMDHLPAVS
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCCHHHH
GWLQHAESAKVVNESRPHANDAAKLSSMVRENVIAQLANIQTHPSVRLAQEKGLLNLHGW
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCEEECCE
VYDIETGSIDALSADRRTFVSLAEQPSTCAVYGQTVDAA
EEEECCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]