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The map label for this gene is phaD [H]

Identifier: 77459534

GI number: 77459534

Start: 3793921

End: 3795600

Strand: Direct

Name: phaD [H]

Synonym: Pfl01_3312

Alternate gene names: 77459534

Gene position: 3793921-3795600 (Clockwise)

Preceding gene: 77459533

Following gene: 77459535

Centisome position: 58.93

GC content: 64.11

Gene sequence:

>1680_bases
ATGACGGCGATGACTCACCTGATCGCCGCACCGATCCTGCTGCCGCTGCTGACCGCCGCCATCATGCTGATGCTCGGCGA
GAAGCACCGACCACTGAAGGCGAAAATCAACCTGTTCTCCAGCCTCCTCGGCCTCGGCATTTCAGTGATGTTGCTGCAAT
GGACCCAGACCACCGGCGTGCCCGGCTCCATCGGCGTGTACCTGCCGGGCAACTGGCAGGTGCCGTTCGGCATCGTGCTG
GTGGTCGATCGTCTGTCGGCATTGATGCTGGTGCTGACCGGGATCATCGGCGTCAGCGCCCTGCTGTTCGCCATGGCCCG
CTGGGACGGCGCCGGGTCGAGCTTCCACGCGTTGTTCCAGATTCAGTTGATGGGACTCTATGGCGCGTTCCTGACGGCGG
ACCTGTTCAACCTGTTCGTGTTCTTCGAGGTGCTGCTCGCTGCGTCCTACGGCCTGCTGCTTCACGGTTCGGGCCGGGCG
CGTGTGTCGTCGGGGCTGCATTACATCTCGATCAACCTGCTGGCCTCGTCGCTGTTTTTGATCGGCGCGGCATTGATCTA
CGGAGTGACCGGCACTCTGAACATGGCGGATCTGGCCCTGAAGATCCCGCTGGTGCCGGAGGCCGACCGTGGCCTGCTGC
ACGCCGGCGCGGGAATTCTGGCCGTGGCGTTCCTGGCCAAGGCCGGCATGTGGCCGCTGAACTTCTGGCTGGTGCCGGCC
TACTCGTCGGCCAGTGCGCCGGTAGCGGCGATGTTCGCGATCATGACTAAAGTCGGCGTCTACACCCTGCTACGTCTGTG
GACGCTGTTGTTCTCGGGCCAGGCCGGCGCTTCGGCGTATTTCGGCGGCGACTGGCTGATCTACGGCGGCATGGCCACCA
TGGCCGGCGCAGGTGTGGCGATCATCGCCGCGCAGCGCCTGGAACGCATGGCCAGCCTGAGCATTCTGGTGTCGGCGGGA
ATTCTGCTGTCTGCCGTGGGGTTCGCCCAGCCGAACCTGATCGGTGCGGCGCTGTTTTATCTGGTCAGCTCGACCCTGGC
GTTGAGCGCACTGTTTCTGCTGGCCGAGTTGATCGAGCGTTCGCGCACCGCCATCGAAGTGCCGCTGGAAGACGAGAACG
AACTGTTGCCGCGTCCGCAAGAGTGGCAACGACCGGTCAAGGGCATCAACCTCGACGACGATCAGAAAGCGCTGGTTGGT
CAGGTGATTCCGTGGACCATGGCTTTCCTCGGTCTGAGCTTCATTGCCTGCGCGTTGCTGATCATCGGCATGCCGCCGCT
GTCCGGGTTCATCGGCAAGCTCAGCCTGATCGGCGCCCTGCTCAATCCGCTGGGCCTCGGCGCCGGCGAACCGATCTCGA
ATGCTGCCTGGGCGTTGCTGGCCCTGCTGATCCTTACCGGCCTCGGCTCGTTGATGGCATTCTCGCGCCTGGGGATCCAG
CGCTTCTGGTCGCCAGAGGAGCGCCCGTCGCCCGTGCTGCGCAAACTGGAATGCACGCCGATTTTCCTGCTGCTCGGCCT
GAGCATCGCCCTGACCTTCAAGGCTGAACCGCTGCTGCGTTACACCCAGGCAGCAGCCGATGCACTGAACAATCCGCAGC
AATACGTAATGGCGGTGCTCGGCACCCGCGCCGTGCCGGGTCCGGAAGCCAAGGCTGCGCTGCTGGAGGTGCAACCATGA

Upstream 100 bases:

>100_bases
TCGTCATCAGCTTTGCCATGACCGCATTGTTCCTGGTGGTGTTGCTCGCCTCACGAGGCCTGACCGGTACCGACCACGTT
GACGGAAGGGAGCCCAAGGA

Downstream 100 bases:

>100_bases
AGCGTCTGTTTCCTGCTCCTTGGCTGTCGCTTGCGCTGTGGTTGCTCTGGCTGGTGCTGAACCTGTCGGTCAGCCCGGGC
AACCTGCTGCTGGGTGCCGT

Product: putative monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MTAMTHLIAAPILLPLLTAAIMLMLGEKHRPLKAKINLFSSLLGLGISVMLLQWTQTTGVPGSIGVYLPGNWQVPFGIVL
VVDRLSALMLVLTGIIGVSALLFAMARWDGAGSSFHALFQIQLMGLYGAFLTADLFNLFVFFEVLLAASYGLLLHGSGRA
RVSSGLHYISINLLASSLFLIGAALIYGVTGTLNMADLALKIPLVPEADRGLLHAGAGILAVAFLAKAGMWPLNFWLVPA
YSSASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAYFGGDWLIYGGMATMAGAGVAIIAAQRLERMASLSILVSAG
ILLSAVGFAQPNLIGAALFYLVSSTLALSALFLLAELIERSRTAIEVPLEDENELLPRPQEWQRPVKGINLDDDQKALVG
QVIPWTMAFLGLSFIACALLIIGMPPLSGFIGKLSLIGALLNPLGLGAGEPISNAAWALLALLILTGLGSLMAFSRLGIQ
RFWSPEERPSPVLRKLECTPIFLLLGLSIALTFKAEPLLRYTQAAADALNNPQQYVMAVLGTRAVPGPEAKAALLEVQP

Sequences:

>Translated_559_residues
MTAMTHLIAAPILLPLLTAAIMLMLGEKHRPLKAKINLFSSLLGLGISVMLLQWTQTTGVPGSIGVYLPGNWQVPFGIVL
VVDRLSALMLVLTGIIGVSALLFAMARWDGAGSSFHALFQIQLMGLYGAFLTADLFNLFVFFEVLLAASYGLLLHGSGRA
RVSSGLHYISINLLASSLFLIGAALIYGVTGTLNMADLALKIPLVPEADRGLLHAGAGILAVAFLAKAGMWPLNFWLVPA
YSSASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAYFGGDWLIYGGMATMAGAGVAIIAAQRLERMASLSILVSAG
ILLSAVGFAQPNLIGAALFYLVSSTLALSALFLLAELIERSRTAIEVPLEDENELLPRPQEWQRPVKGINLDDDQKALVG
QVIPWTMAFLGLSFIACALLIIGMPPLSGFIGKLSLIGALLNPLGLGAGEPISNAAWALLALLILTGLGSLMAFSRLGIQ
RFWSPEERPSPVLRKLECTPIFLLLGLSIALTFKAEPLLRYTQAAADALNNPQQYVMAVLGTRAVPGPEAKAALLEVQP
>Mature_558_residues
TAMTHLIAAPILLPLLTAAIMLMLGEKHRPLKAKINLFSSLLGLGISVMLLQWTQTTGVPGSIGVYLPGNWQVPFGIVLV
VDRLSALMLVLTGIIGVSALLFAMARWDGAGSSFHALFQIQLMGLYGAFLTADLFNLFVFFEVLLAASYGLLLHGSGRAR
VSSGLHYISINLLASSLFLIGAALIYGVTGTLNMADLALKIPLVPEADRGLLHAGAGILAVAFLAKAGMWPLNFWLVPAY
SSASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAYFGGDWLIYGGMATMAGAGVAIIAAQRLERMASLSILVSAGI
LLSAVGFAQPNLIGAALFYLVSSTLALSALFLLAELIERSRTAIEVPLEDENELLPRPQEWQRPVKGINLDDDQKALVGQ
VIPWTMAFLGLSFIACALLIIGMPPLSGFIGKLSLIGALLNPLGLGAGEPISNAAWALLALLILTGLGSLMAFSRLGIQR
FWSPEERPSPVLRKLECTPIFLLLGLSIALTFKAEPLLRYTQAAADALNNPQQYVMAVLGTRAVPGPEAKAALLEVQP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788827, Length=389, Percent_Identity=27.2493573264781, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788613, Length=333, Percent_Identity=29.7297297297297, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 59270; Mature: 59139

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAMTHLIAAPILLPLLTAAIMLMLGEKHRPLKAKINLFSSLLGLGISVMLLQWTQTTGV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGSIGVYLPGNWQVPFGIVLVVDRLSALMLVLTGIIGVSALLFAMARWDGAGSSFHALFQ
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IQLMGLYGAFLTADLFNLFVFFEVLLAASYGLLLHGSGRARVSSGLHYISINLLASSLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEEHHHHHHHHHH
IGAALIYGVTGTLNMADLALKIPLVPEADRGLLHAGAGILAVAFLAKAGMWPLNFWLVPA
HHHHHHHHCCCCCCHHHHEEECCCCCCCCCCHHHHCHHHHHHHHHHHCCCCEEEEEEEEC
YSSASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAYFGGDWLIYGGMATMAGAGVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEECCHHHHHCCCHH
IIAAQRLERMASLSILVSAGILLSAVGFAQPNLIGAALFYLVSSTLALSALFLLAELIER
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SRTAIEVPLEDENELLPRPQEWQRPVKGINLDDDQKALVGQVIPWTMAFLGLSFIACALL
CCCEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGMPPLSGFIGKLSLIGALLNPLGLGAGEPISNAAWALLALLILTGLGSLMAFSRLGIQ
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFWSPEERPSPVLRKLECTPIFLLLGLSIALTFKAEPLLRYTQAAADALNNPQQYVMAVL
HHCCCCCCCCHHHHHCCCCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCHHHHHHHHH
GTRAVPGPEAKAALLEVQP
HCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
TAMTHLIAAPILLPLLTAAIMLMLGEKHRPLKAKINLFSSLLGLGISVMLLQWTQTTGV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGSIGVYLPGNWQVPFGIVLVVDRLSALMLVLTGIIGVSALLFAMARWDGAGSSFHALFQ
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
IQLMGLYGAFLTADLFNLFVFFEVLLAASYGLLLHGSGRARVSSGLHYISINLLASSLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCEEEHHHHHHHHHH
IGAALIYGVTGTLNMADLALKIPLVPEADRGLLHAGAGILAVAFLAKAGMWPLNFWLVPA
HHHHHHHHCCCCCCHHHHEEECCCCCCCCCCHHHHCHHHHHHHHHHHCCCCEEEEEEEEC
YSSASAPVAAMFAIMTKVGVYTLLRLWTLLFSGQAGASAYFGGDWLIYGGMATMAGAGVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCEEEECCHHHHHCCCHH
IIAAQRLERMASLSILVSAGILLSAVGFAQPNLIGAALFYLVSSTLALSALFLLAELIER
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SRTAIEVPLEDENELLPRPQEWQRPVKGINLDDDQKALVGQVIPWTMAFLGLSFIACALL
CCCEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGMPPLSGFIGKLSLIGALLNPLGLGAGEPISNAAWALLALLILTGLGSLMAFSRLGIQ
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFWSPEERPSPVLRKLECTPIFLLLGLSIALTFKAEPLLRYTQAAADALNNPQQYVMAVL
HHCCCCCCCCHHHHHCCCCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCHHHHHHHHH
GTRAVPGPEAKAALLEVQP
HCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]