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The map label for this gene is sbcD [H]

Identifier: 77459438

GI number: 77459438

Start: 3695617

End: 3696861

Strand: Direct

Name: sbcD [H]

Synonym: Pfl01_3216

Alternate gene names: 77459438

Gene position: 3695617-3696861 (Clockwise)

Preceding gene: 77459435

Following gene: 77459439

Centisome position: 57.4

GC content: 63.61

Gene sequence:

>1245_bases
TTGCGTCTGTTTCACACCTCCGACTGGCACCTTGGGCAGAACCTCCACGGGCAGGAGCGCGATTTCGAGCACGCCTGTTT
TCTCGAGTGGCTGCTGCGCCAATTGAAGCTGGCGCAGCCGGACGTGCTGCTGATCGCCGGCGACATCTTCGACACCGTCA
ACCCGCCGGTCAAAGCCCAGGAACGCCTCTACGATTTCATCGTCAGCGCCCACGAACAACAGCCGTTGCTGACCATCGTG
ATGATCGCCGGCAACCACGATTCCGGCTCGCGGATCGAACTGCCGGCGCCGTTGATGCGGCGTTTGCGTACCCATGCGCT
GGGCCGGGTGTTGTGGCTGGATGACGGGCAGCTGGATGCGGAACGTCTGCTGCTGCCGCTGCCGAATGCTTCCGGCGAGA
TCGCCGCGTGGTGCCTGGCGCTGCCGTTCCTGCGGCCTGCCGAAGTGACCGGCGCGCATCTGGGCGATAACTATTTGCGC
GGGATCGGTCAGGTGCATGAATGGCTGATCGAAGCGGCGAACGCCAAACGCCAGCCGGGCCAGGCACTGGTCGCCATCAG
CCATGCGCACATGGCCGGCGGCTCGGTGTCGGAAGACTCCGAGCGCAGCCTGATCATCGGCAACGCCGAAGCCCTGCCTG
CCAGCCTGTTCGGGCCGAGCATCAGCTATGTCGCCCTCGGCCATTTGCACAAGCCGCAGAAGGTCAATGGCGAAGAAAGA
ATTCGCTACAGCGGCTCGCCGATCCCGTTATCGTTCTCGGAAATCGCCTATCAACATCAGATTCTCGACATCGTGCTCGA
CGGCGAAACCCTGGTCAGCGTCGAGCCAAAACTGATCCCTCGCGCGGTCAATCTGCAACGCCTCGGCCCTGCGCCGCTGG
CCGAAATCCTGCTGCAACTGGCGGATCTGCCGAACATCGACCTGCTGGCCGAAACCCAGCGTCAGCCGTGGCTGGAAGTG
CGGGTGCGCCTCGACGAACCGCAGCCGGATCTGCGCCATCAGGTCGAAAGCGCCTTGCAAGGCAAAGCCGTACGACTGGT
GCGGATCGCCGCCGAATACGCCGGCAATCGGGGCGCTGACGGCGCCGAGGACGGCAGCGCGCTGATCGAACTGGACCAGC
TCACGCCGCAGGAATTGTTCAGCCGCGCCTGGCTCGACAATTACGGCAACGAGGTCGACGAGCAAACCCTCAAGGACTTC
GCCGAGCTGCTGCAAGACGTACAAATGGAGGGCGAGCAGCCATGA

Upstream 100 bases:

>100_bases
CCGGACAAGCCTACCCGTCGCAATAATCTGTAAACTCTCCCCTCTTTCGCCGCCCTTCTCATTCCGGCGGCCACCACCTC
TTTATATTGCGGAGTCAGCC

Downstream 100 bases:

>100_bases
AGATTCTCGCCATTCGGTTGAAGAACCTCGCCTCGCTGGCCGGCCCGTTCGAGATCGACTTTACCGCCGAACCGCTGGCC
AGCGCCGGCCTGTTCGCGAT

Product: exodeoxyribonuclease I subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRLFHTSDWHLGQNLHGQERDFEHACFLEWLLRQLKLAQPDVLLIAGDIFDTVNPPVKAQERLYDFIVSAHEQQPLLTIV
MIAGNHDSGSRIELPAPLMRRLRTHALGRVLWLDDGQLDAERLLLPLPNASGEIAAWCLALPFLRPAEVTGAHLGDNYLR
GIGQVHEWLIEAANAKRQPGQALVAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSISYVALGHLHKPQKVNGEER
IRYSGSPIPLSFSEIAYQHQILDIVLDGETLVSVEPKLIPRAVNLQRLGPAPLAEILLQLADLPNIDLLAETQRQPWLEV
RVRLDEPQPDLRHQVESALQGKAVRLVRIAAEYAGNRGADGAEDGSALIELDQLTPQELFSRAWLDNYGNEVDEQTLKDF
AELLQDVQMEGEQP

Sequences:

>Translated_414_residues
MRLFHTSDWHLGQNLHGQERDFEHACFLEWLLRQLKLAQPDVLLIAGDIFDTVNPPVKAQERLYDFIVSAHEQQPLLTIV
MIAGNHDSGSRIELPAPLMRRLRTHALGRVLWLDDGQLDAERLLLPLPNASGEIAAWCLALPFLRPAEVTGAHLGDNYLR
GIGQVHEWLIEAANAKRQPGQALVAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSISYVALGHLHKPQKVNGEER
IRYSGSPIPLSFSEIAYQHQILDIVLDGETLVSVEPKLIPRAVNLQRLGPAPLAEILLQLADLPNIDLLAETQRQPWLEV
RVRLDEPQPDLRHQVESALQGKAVRLVRIAAEYAGNRGADGAEDGSALIELDQLTPQELFSRAWLDNYGNEVDEQTLKDF
AELLQDVQMEGEQP
>Mature_414_residues
MRLFHTSDWHLGQNLHGQERDFEHACFLEWLLRQLKLAQPDVLLIAGDIFDTVNPPVKAQERLYDFIVSAHEQQPLLTIV
MIAGNHDSGSRIELPAPLMRRLRTHALGRVLWLDDGQLDAERLLLPLPNASGEIAAWCLALPFLRPAEVTGAHLGDNYLR
GIGQVHEWLIEAANAKRQPGQALVAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSISYVALGHLHKPQKVNGEER
IRYSGSPIPLSFSEIAYQHQILDIVLDGETLVSVEPKLIPRAVNLQRLGPAPLAEILLQLADLPNIDLLAETQRQPWLEV
RVRLDEPQPDLRHQVESALQGKAVRLVRIAAEYAGNRGADGAEDGSALIELDQLTPQELFSRAWLDNYGNEVDEQTLKDF
AELLQDVQMEGEQP

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=395, Percent_Identity=31.3924050632911, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 45938; Mature: 45938

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFHTSDWHLGQNLHGQERDFEHACFLEWLLRQLKLAQPDVLLIAGDIFDTVNPPVKAQ
CCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHH
ERLYDFIVSAHEQQPLLTIVMIAGNHDSGSRIELPAPLMRRLRTHALGRVLWLDDGQLDA
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHCEEEEECCCCCCC
ERLLLPLPNASGEIAAWCLALPFLRPAEVTGAHLGDNYLRGIGQVHEWLIEAANAKRQPG
CCEEEECCCCCCHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
QALVAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSISYVALGHLHKPQKVNGEER
CEEEEEEEHHHCCCCCCCCCCCEEEEECCCCCCHHHCCCCCHHEEECCCCCCCCCCCHHH
IRYSGSPIPLSFSEIAYQHQILDIVLDGETLVSVEPKLIPRAVNLQRLGPAPLAEILLQL
EEECCCCCCCCHHHHHHHHEEEEEEECCCEEEEECHHHCCCCCCHHHCCCCHHHHHHHHH
ADLPNIDLLAETQRQPWLEVRVRLDEPQPDLRHQVESALQGKAVRLVRIAAEYAGNRGAD
HCCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
GAEDGSALIELDQLTPQELFSRAWLDNYGNEVDEQTLKDFAELLQDVQMEGEQP
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLFHTSDWHLGQNLHGQERDFEHACFLEWLLRQLKLAQPDVLLIAGDIFDTVNPPVKAQ
CCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHH
ERLYDFIVSAHEQQPLLTIVMIAGNHDSGSRIELPAPLMRRLRTHALGRVLWLDDGQLDA
HHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHCEEEEECCCCCCC
ERLLLPLPNASGEIAAWCLALPFLRPAEVTGAHLGDNYLRGIGQVHEWLIEAANAKRQPG
CCEEEECCCCCCHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
QALVAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSISYVALGHLHKPQKVNGEER
CEEEEEEEHHHCCCCCCCCCCCEEEEECCCCCCHHHCCCCCHHEEECCCCCCCCCCCHHH
IRYSGSPIPLSFSEIAYQHQILDIVLDGETLVSVEPKLIPRAVNLQRLGPAPLAEILLQL
EEECCCCCCCCHHHHHHHHEEEEEEECCCEEEEECHHHCCCCCCHHHCCCCHHHHHHHHH
ADLPNIDLLAETQRQPWLEVRVRLDEPQPDLRHQVESALQGKAVRLVRIAAEYAGNRGAD
HCCCCCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
GAEDGSALIELDQLTPQELFSRAWLDNYGNEVDEQTLKDFAELLQDVQMEGEQP
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]