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The map label for this gene is gacS [H]

Identifier: 77459359

GI number: 77459359

Start: 3598963

End: 3599838

Strand: Direct

Name: gacS [H]

Synonym: Pfl01_3137

Alternate gene names: 77459359

Gene position: 3598963-3599838 (Clockwise)

Preceding gene: 77459358

Following gene: 77459360

Centisome position: 55.9

GC content: 61.3

Gene sequence:

>876_bases
ATGACTGAGTCCCCGACCTTGAACAACGCCGAACAGGCTGCGTTGATCGCCCAGTTGCAGAGCGAAACCGCTGCGTTGCG
CGAAGAGCTCGACGAAACCAATCAGGGCGTGCTGGCCCTGTACGCCGAACTCGACACTCAGGCCGAACAACTGCGCCAGG
CATCCGACCTGAAAAGCCGCTTCCTGTCGTACATGAGTCACGAGTTCCGCACGCCGCTGGGCTCGATCCTGAGCATCAAC
AGCCTGCTCGCCGACGAACTCGACGGCCCCCTCAGCCCCGAGCAACACAAGCAGGTGGCGTTTGTCAGCACCGCCGCCCG
GGAACTCAGCGACATGGTCGACGACCTGCTCGATCTGGCGAAGATCGAGGCCGGACGCATCAGCATTTCTGCGGCGTGGT
TCGACATGTTCGACCTGTTCGCCGCACTGCGGGGGATGTTCCGGCCGATTGTCGATGCCACGGCCGTAGACCTGATTTTC
GAAGAGCCGGTAGGCCTCCCGCGTCTTTACACCGACGACAAGAAGCTGGCGCAGATCCTGCGCAACTTCATTTCCAACTC
GCTGAAGTTCACCACCCGTGGCGAGGTCCGGATCTCGGCGCGTCTTGAGGGTGAGGGCAAGGTGCGGTTCGCCGTCAGCG
ACACCGGCATCGGTATCGCTGCGGAGCTGCACGGCACCTTGTTTGAAGACTTTTCCCAGGTCGACTCACCGCTGCAAAAA
CGCCTGCGCGGCACCGGGCTCGGCCTGTCGCTGTGCAAACGCTTCGCTGAATTGCTCGGTGGTGAGGTTGGTGTCGAAAG
TACGCCGGGCGTGGGCTCAAGCTTTTTCGTGATCATTCCGCTGGCACTTGCCCAGGAGAACGTCGATGAAACATGA

Upstream 100 bases:

>100_bases
GTGCATCGCCATCCCGCCGTGATTGCCACCGTCCTGCACCGCGACTTCTGCCGCGGGCGCGACGATGTCACCGTGCTGGT
GGTTGCCCTGGAGTCCGCCC

Downstream 100 bases:

>100_bases
CATCCGTCTGTTGATCGTCGATGACAACGTCGCCACTCGCTATGCCTTGCGCCGACGACTGGAGCGCCACGGTTACGAAG
TGCTCGAGGCCGGCACCGGC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTESPTLNNAEQAALIAQLQSETAALREELDETNQGVLALYAELDTQAEQLRQASDLKSRFLSYMSHEFRTPLGSILSIN
SLLADELDGPLSPEQHKQVAFVSTAARELSDMVDDLLDLAKIEAGRISISAAWFDMFDLFAALRGMFRPIVDATAVDLIF
EEPVGLPRLYTDDKKLAQILRNFISNSLKFTTRGEVRISARLEGEGKVRFAVSDTGIGIAAELHGTLFEDFSQVDSPLQK
RLRGTGLGLSLCKRFAELLGGEVGVESTPGVGSSFFVIIPLALAQENVDET

Sequences:

>Translated_291_residues
MTESPTLNNAEQAALIAQLQSETAALREELDETNQGVLALYAELDTQAEQLRQASDLKSRFLSYMSHEFRTPLGSILSIN
SLLADELDGPLSPEQHKQVAFVSTAARELSDMVDDLLDLAKIEAGRISISAAWFDMFDLFAALRGMFRPIVDATAVDLIF
EEPVGLPRLYTDDKKLAQILRNFISNSLKFTTRGEVRISARLEGEGKVRFAVSDTGIGIAAELHGTLFEDFSQVDSPLQK
RLRGTGLGLSLCKRFAELLGGEVGVESTPGVGSSFFVIIPLALAQENVDET
>Mature_290_residues
TESPTLNNAEQAALIAQLQSETAALREELDETNQGVLALYAELDTQAEQLRQASDLKSRFLSYMSHEFRTPLGSILSINS
LLADELDGPLSPEQHKQVAFVSTAARELSDMVDDLLDLAKIEAGRISISAAWFDMFDLFAALRGMFRPIVDATAVDLIFE
EPVGLPRLYTDDKKLAQILRNFISNSLKFTTRGEVRISARLEGEGKVRFAVSDTGIGIAAELHGTLFEDFSQVDSPLQKR
LRGTGLGLSLCKRFAELLGGEVGVESTPGVGSSFFVIIPLALAQENVDET

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=34.4262295081967, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=33.4710743801653, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1788713, Length=251, Percent_Identity=30.6772908366534, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081816, Length=291, Percent_Identity=34.7079037800687, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789149, Length=267, Percent_Identity=31.0861423220974, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786912, Length=247, Percent_Identity=26.3157894736842, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082128, Length=260, Percent_Identity=27.6923076923077, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788393, Length=243, Percent_Identity=27.1604938271605, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=26.7857142857143, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786600, Length=244, Percent_Identity=27.0491803278689, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=258, Percent_Identity=24.8062015503876, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790346, Length=260, Percent_Identity=26.1538461538462, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786783, Length=225, Percent_Identity=25.3333333333333, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=79, Percent_Identity=40.5063291139241, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 31782; Mature: 31651

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESPTLNNAEQAALIAQLQSETAALREELDETNQGVLALYAELDTQAEQLRQASDLKSR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
FLSYMSHEFRTPLGSILSINSLLADELDGPLSPEQHKQVAFVSTAARELSDMVDDLLDLA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIEAGRISISAAWFDMFDLFAALRGMFRPIVDATAVDLIFEEPVGLPRLYTDDKKLAQIL
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RNFISNSLKFTTRGEVRISARLEGEGKVRFAVSDTGIGIAAELHGTLFEDFSQVDSPLQK
HHHHCCCCEEEECCCEEEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHH
RLRGTGLGLSLCKRFAELLGGEVGVESTPGVGSSFFVIIPLALAQENVDET
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TESPTLNNAEQAALIAQLQSETAALREELDETNQGVLALYAELDTQAEQLRQASDLKSR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
FLSYMSHEFRTPLGSILSINSLLADELDGPLSPEQHKQVAFVSTAARELSDMVDDLLDLA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIEAGRISISAAWFDMFDLFAALRGMFRPIVDATAVDLIFEEPVGLPRLYTDDKKLAQIL
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RNFISNSLKFTTRGEVRISARLEGEGKVRFAVSDTGIGIAAELHGTLFEDFSQVDSPLQK
HHHHCCCCEEEECCCEEEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHH
RLRGTGLGLSLCKRFAELLGGEVGVESTPGVGSSFFVIIPLALAQENVDET
HHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]