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The map label for this gene is yqiR [H]

Identifier: 77459299

GI number: 77459299

Start: 3520782

End: 3522209

Strand: Direct

Name: yqiR [H]

Synonym: Pfl01_3077

Alternate gene names: 77459299

Gene position: 3520782-3522209 (Clockwise)

Preceding gene: 77459298

Following gene: 77459300

Centisome position: 54.68

GC content: 61.9

Gene sequence:

>1428_bases
ATGGACGCCGCCATGAACACCACCGAAAGCCTCAAGGATTACCAACGGGTTCGCGCCCTGGCGATCCGTTCGTTGTTCGA
GATCATCGAGCAATCCAGTGAGGGCACGGTGATTGTCGACCGCGACGCGAACATCGTCTGGATGAACGAGCGCTACGCTC
GGCGCTTCGGGCTCGACTCCGCCGCCGGGGCCATCGGCAAGCCTTGCGAAAGCGTGATCCCCGGCAGCCTGTTGCGTGAA
GTGGTACGCACCGGTCGGCCGATTCTGCTGGACATGCAGGACACCCCGAAAGAACCGCTGGTGGTGATGCGCCTGCCGAT
CCATGACGACGCCGGCGCGGTGATCGGCGCCATCGGTTTTGCCCTGTTCGACGAACTGCGCACGCTGTCGCCGATGCTCA
AGCGCTACCTGAGCATGCAGGAAGAACTGGCCTCGACCCGTTCACTGCTGCGCGCCCGGCAGACCAAATACAACTTCGCG
CACTTCATCGGCACCAGCGCTGCCAGCCTGGAAGTCAAACGCCGTGCCCGTCGCAGCGCCAGTGCCGAGTCGCCGGTTCT
GTTGCTGGGCGAAACCGGCAGCGGCAAGGAGTTGCTGGCGCAAGCGATCCACGGCGCTTCGCCTCGGGCGCACAAGGCGT
TCGTCAGCATCAACAGCGCAGCGATTCCCGAGGCGCTGCTCGAGGCCGAGTTCTTCGGCACCGCGCCCGGCGCCTTCACC
GGCGCCGACCGCAAGGGCCGCACCGGCAAGTTGCAGATTGCTCAGGGCGGGACGCTGTTTCTCGACGAGATCGGCGACAT
GCCGCTGCCCTTGCAAAGCAAATTGTTGCGGGTGTTGCAGGAAAAGGAGTTCGAACCTGTAGGCTCCAACGACGTGATCC
AGAGCGATGTGCGGGTGATTGCGGCGACCTCGACGGATCTGGAGGCGGCGATCAAGCGCGGCGAATTCCGCGCCGACTTG
TATTACCGCCTCAACGTATTGCCAATTCAGGTGCCGCCACTGCGTGAACGACTGGACGATCTGCCGGCCCTCAGTGAGGC
GATTCTTGAAGAACTGCGCAGTCAACATGAACTGAACCGCGAAGCGCTGGAGCTGCTGGGTCAGCATGCCTGGCCAGGCA
ATATCCGCGAGCTGCGCAATGTATTGGAGCGGGCAGCGTTGCTCAGCGATGACTTGATGCTGACGGCGGATGACATTCGC
TCTGCAATTGGAACGTTTACACCGGTTGAGCGAACGCTGGCACCCTTGCCTGAACCGGCAATCAACGAGACCTTCAGTCA
AGCCCGGGAGCGTTTTGACCGGCAGTTGATTCAATCCACCCTCGCGCAATGCGGGGGCAAGGTGATTGAGGCGGCTGAGC
GGTTGGGACTGGGCCGTTCGACGCTTTACAAGAAAATGATTGCGCTGGGGATCGCAGAGTCTCAATAA

Upstream 100 bases:

>100_bases
AGCCATGACGTGTTCGATTCACCGCAGCATCCCTATACCAAAGAGCTGTTGGCCGCGGCGCATCCGGGATAGGCATAATC
GCGCCACCTGACTTACGCCA

Downstream 100 bases:

>100_bases
GGAGACACAGCTCTCCAAATCAAGACAACATCAAAAGCATCGCGGGCAAGCCCGCTCCCACAGGGGAATGCGTTCAACTG
TGGGAGCGGGCTTGCCCGCG

Product: sigma54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MDAAMNTTESLKDYQRVRALAIRSLFEIIEQSSEGTVIVDRDANIVWMNERYARRFGLDSAAGAIGKPCESVIPGSLLRE
VVRTGRPILLDMQDTPKEPLVVMRLPIHDDAGAVIGAIGFALFDELRTLSPMLKRYLSMQEELASTRSLLRARQTKYNFA
HFIGTSAASLEVKRRARRSASAESPVLLLGETGSGKELLAQAIHGASPRAHKAFVSINSAAIPEALLEAEFFGTAPGAFT
GADRKGRTGKLQIAQGGTLFLDEIGDMPLPLQSKLLRVLQEKEFEPVGSNDVIQSDVRVIAATSTDLEAAIKRGEFRADL
YYRLNVLPIQVPPLRERLDDLPALSEAILEELRSQHELNREALELLGQHAWPGNIRELRNVLERAALLSDDLMLTADDIR
SAIGTFTPVERTLAPLPEPAINETFSQARERFDRQLIQSTLAQCGGKVIEAAERLGLGRSTLYKKMIALGIAESQ

Sequences:

>Translated_475_residues
MDAAMNTTESLKDYQRVRALAIRSLFEIIEQSSEGTVIVDRDANIVWMNERYARRFGLDSAAGAIGKPCESVIPGSLLRE
VVRTGRPILLDMQDTPKEPLVVMRLPIHDDAGAVIGAIGFALFDELRTLSPMLKRYLSMQEELASTRSLLRARQTKYNFA
HFIGTSAASLEVKRRARRSASAESPVLLLGETGSGKELLAQAIHGASPRAHKAFVSINSAAIPEALLEAEFFGTAPGAFT
GADRKGRTGKLQIAQGGTLFLDEIGDMPLPLQSKLLRVLQEKEFEPVGSNDVIQSDVRVIAATSTDLEAAIKRGEFRADL
YYRLNVLPIQVPPLRERLDDLPALSEAILEELRSQHELNREALELLGQHAWPGNIRELRNVLERAALLSDDLMLTADDIR
SAIGTFTPVERTLAPLPEPAINETFSQARERFDRQLIQSTLAQCGGKVIEAAERLGLGRSTLYKKMIALGIAESQ
>Mature_475_residues
MDAAMNTTESLKDYQRVRALAIRSLFEIIEQSSEGTVIVDRDANIVWMNERYARRFGLDSAAGAIGKPCESVIPGSLLRE
VVRTGRPILLDMQDTPKEPLVVMRLPIHDDAGAVIGAIGFALFDELRTLSPMLKRYLSMQEELASTRSLLRARQTKYNFA
HFIGTSAASLEVKRRARRSASAESPVLLLGETGSGKELLAQAIHGASPRAHKAFVSINSAAIPEALLEAEFFGTAPGAFT
GADRKGRTGKLQIAQGGTLFLDEIGDMPLPLQSKLLRVLQEKEFEPVGSNDVIQSDVRVIAATSTDLEAAIKRGEFRADL
YYRLNVLPIQVPPLRERLDDLPALSEAILEELRSQHELNREALELLGQHAWPGNIRELRNVLERAALLSDDLMLTADDIR
SAIGTFTPVERTLAPLPEPAINETFSQARERFDRQLIQSTLAQCGGKVIEAAERLGLGRSTLYKKMIALGIAESQ

Specific function: Required For Induction Of Expression Of The Formate Dehydrogenase H And Hydrogenase-3 Structural Genes. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=346, Percent_Identity=44.7976878612717, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1788550, Length=367, Percent_Identity=39.7820163487738, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1788905, Length=300, Percent_Identity=44.3333333333333, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082117, Length=306, Percent_Identity=46.078431372549, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=40.625, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1790437, Length=340, Percent_Identity=40.2941176470588, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=34.6153846153846, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786524, Length=342, Percent_Identity=42.1052631578947, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87082152, Length=322, Percent_Identity=40.9937888198758, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1787583, Length=349, Percent_Identity=32.9512893982808, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI87081872, Length=312, Percent_Identity=37.8205128205128, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI87081858, Length=464, Percent_Identity=28.8793103448276, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789828, Length=287, Percent_Identity=33.7979094076655, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR002197
- InterPro:   IPR016040
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52228; Mature: 52228

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAMNTTESLKDYQRVRALAIRSLFEIIEQSSEGTVIVDRDANIVWMNERYARRFGLDS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECHHHHHHHCCCH
AAGAIGKPCESVIPGSLLREVVRTGRPILLDMQDTPKEPLVVMRLPIHDDAGAVIGAIGF
HCCCCCCCHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHH
ALFDELRTLSPMLKRYLSMQEELASTRSLLRARQTKYNFAHFIGTSAASLEVKRRARRSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
SAESPVLLLGETGSGKELLAQAIHGASPRAHKAFVSINSAAIPEALLEAEFFGTAPGAFT
CCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHEECCHHHHHHHHHHHHHHCCCCCCCC
GADRKGRTGKLQIAQGGTLFLDEIGDMPLPLQSKLLRVLQEKEFEPVGSNDVIQSDVRVI
CCCCCCCCCEEEEECCCEEEHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEE
AATSTDLEAAIKRGEFRADLYYRLNVLPIQVPPLRERLDDLPALSEAILEELRSQHELNR
EECCHHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHCCH
EALELLGQHAWPGNIRELRNVLERAALLSDDLMLTADDIRSAIGTFTPVERTLAPLPEPA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHCCCCCHH
INETFSQARERFDRQLIQSTLAQCGGKVIEAAERLGLGRSTLYKKMIALGIAESQ
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDAAMNTTESLKDYQRVRALAIRSLFEIIEQSSEGTVIVDRDANIVWMNERYARRFGLDS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECHHHHHHHCCCH
AAGAIGKPCESVIPGSLLREVVRTGRPILLDMQDTPKEPLVVMRLPIHDDAGAVIGAIGF
HCCCCCCCHHHHCCHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHH
ALFDELRTLSPMLKRYLSMQEELASTRSLLRARQTKYNFAHFIGTSAASLEVKRRARRSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
SAESPVLLLGETGSGKELLAQAIHGASPRAHKAFVSINSAAIPEALLEAEFFGTAPGAFT
CCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHEECCHHHHHHHHHHHHHHCCCCCCCC
GADRKGRTGKLQIAQGGTLFLDEIGDMPLPLQSKLLRVLQEKEFEPVGSNDVIQSDVRVI
CCCCCCCCCEEEEECCCEEEHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCEEE
AATSTDLEAAIKRGEFRADLYYRLNVLPIQVPPLRERLDDLPALSEAILEELRSQHELNR
EECCHHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHCCH
EALELLGQHAWPGNIRELRNVLERAALLSDDLMLTADDIRSAIGTFTPVERTLAPLPEPA
HHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHCCCCCHH
INETFSQARERFDRQLIQSTLAQCGGKVIEAAERLGLGRSTLYKKMIALGIAESQ
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]