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The map label for this gene is topB [H]

Identifier: 77459223

GI number: 77459223

Start: 3442472

End: 3444439

Strand: Direct

Name: topB [H]

Synonym: Pfl01_3001

Alternate gene names: 77459223

Gene position: 3442472-3444439 (Clockwise)

Preceding gene: 77459222

Following gene: 77459224

Centisome position: 53.47

GC content: 58.64

Gene sequence:

>1968_bases
ATGCGTCTCTTCCTCTGCGAGAAACCATCCCAAGGTCGTGACATCGCCAAGGTTCTCGGGGCCACTCGGCGTGGTGACGG
TTGCCTGATCGGCACCGACTCAACCGTCACCTGGTGTATCGGTCACCTGCTGGAGACGGCCCCACCTGAAGCTTATGGTG
CTCAGTACAAATCCTGGTCGCTGGATCATCTACCGATCATTCCCGCGCAGTGGCAGGTCGAGGTCAAATCCAAGACCGCC
GCGCAGTTCAAAATCATCAAACGCCTGCTGAGTGAAGCCTCAAGTGTTGTCATCGCGACCGACGCCGATCGCGAAGGTGA
AATGATTGCCCGCGAGCTGCTTGAACTCTGCAGGTATCGAGGCCCCGTTCTGCGCCTCTGGTTGTCCGCCCTCAACGAGG
CGTCTATTCGCAAAGCCTTGTCCTCGCTGAAGTCCGGTCAGGTGACTTTCCCGCTCTATCACTCGGCGCTCGCCCGCAGC
CGTGCCGACTGGCTGATCGGCATGAACCTCAGTCGTTTGTTTACCCTTCTCGGTCGGCGAGCAGGTTACGACGGCGTTTT
GTCAGTGGGGCGCGTCCAGACACCCACTTTACGTCTGGTGGTCGATCGGGATCGTGCGATTGCCAGCTTCGTCCCGGTGC
CTTACTGGACCGTTGACGTGCACCTATCGTCGATGGGCCAGCGATTCATCGCGTCGTGGTTACCACCACGTTCAGGACGG
GACGAAGCGGGGCGTTGCCTGCAACAGGCGCTCGCCCATCACGCGGTCCAAACAATCACCGGCGAGAAGTCCGCCACGGT
ACTCTCGTTGCAAACTGAGCAGCTCCGAGAACCGCCGCCCCTGCCCTTCGACCTGAGCACGTTGCAAGAAGTCTGCTCGC
GTAAGCTGGGGCTCGGTGTTCAGGAAACCCTGAACATCGCCCAAGCCCTGTATGAAACCCACAAAGCCACCACCTACCCG
CGCAGCGATTGCCGCTATTTGCCAGAGAGCATGTTCAACGAGGCACCCGCGGTACTCGACGCCCTGCTCAAAACGGATCC
TGCACTCAGACCTGCATTCGGGAGCCTCGATCGATCATTGCGCGCCCGCGTATGGAACAATGCTAAGGTCACCGCGCACC
ACGGCATCATTCCCACCACCGAGCCCGCAAATTTGGTGCGGATGTCCGAACAAGAACGCCAAGTCTACGAACTGATCCGC
AGCCACTACCTGGCGCAGTTTCTTCCGCATCATGAGTTTGATCGAACCCAGGTCGAACTGGAATGTAGCAAAGAACGTTT
GACCGCTGTCGGCAAACAGATCCTGATCCAGGGCTGGAAAGGCTTGCTCACCGAAAACACCGAGGACGATGAGCCCAGTC
AAAAGCACCAAGTATTGCCAGTCCTGCAACAGGGTACTCAGTGCGCCGTAAACGACGTCGAACTCAAGTCAATGCGCACC
GTCGCTCCCAAACCGCTCACCGAGGGCGATCTGATCAAGGCGATGAAAAACGTCGCCAAGCTGGTCAACGACCCACGCCT
GAAACAGAAACTTCGGGACACCACCGGTATCGGTACCGAAGCCACGCGAGCCGGCATTATCAAAGGGTTGGTTGATCGCG
GTTATTTGCTGAAGAAAAAACGCGCCTTAATGGCCTCCGCCGCGGCCCATACCCTGATCGAGGCGGCACCGACAGCAGTC
GCCGATCCGGGTATGACCGCAATCTGGGAACAGGCGCTGGACGAAATCGAAGCGGGACGCCTGACCCTGGATGCGTTCGT
TGCCAAGCAGGCGAACTGGATTGCGCAGTTGGTCGCACACTACCGCTCGCACACTTTGGCAATAGCGGTCGAAACGAGCC
CGAGCTGCCCTATGTGCAACGCCTCAATGCTCAGGCGAAAGGGAAAATCAGGGCCGTTCTGGTCGTGCTCCTGCTACCCG
GATTGCAAGGGCACTGTGTCGATCAGTAAAGATACGCGTCGCTCATGA

Upstream 100 bases:

>100_bases
GCCCTCACCGACTATATGAGGAACCTGCCATTCAGGTTTCTCATATCTCGCCCGCGCTACTTCGAACAACGCTGCTCCTA
AATCAATCCGGAGCAGCTCT

Downstream 100 bases:

>100_bases
TGGTCCTGGGGCGATCATCATGAGAATGGTGCGCTCTCTGCATTGACCTCGTCCAATCCTCTCTGCTTTAGTACCTCAGG
ATCATCGCCAACGGCGACCC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MRLFLCEKPSQGRDIAKVLGATRRGDGCLIGTDSTVTWCIGHLLETAPPEAYGAQYKSWSLDHLPIIPAQWQVEVKSKTA
AQFKIIKRLLSEASSVVIATDADREGEMIARELLELCRYRGPVLRLWLSALNEASIRKALSSLKSGQVTFPLYHSALARS
RADWLIGMNLSRLFTLLGRRAGYDGVLSVGRVQTPTLRLVVDRDRAIASFVPVPYWTVDVHLSSMGQRFIASWLPPRSGR
DEAGRCLQQALAHHAVQTITGEKSATVLSLQTEQLREPPPLPFDLSTLQEVCSRKLGLGVQETLNIAQALYETHKATTYP
RSDCRYLPESMFNEAPAVLDALLKTDPALRPAFGSLDRSLRARVWNNAKVTAHHGIIPTTEPANLVRMSEQERQVYELIR
SHYLAQFLPHHEFDRTQVELECSKERLTAVGKQILIQGWKGLLTENTEDDEPSQKHQVLPVLQQGTQCAVNDVELKSMRT
VAPKPLTEGDLIKAMKNVAKLVNDPRLKQKLRDTTGIGTEATRAGIIKGLVDRGYLLKKKRALMASAAAHTLIEAAPTAV
ADPGMTAIWEQALDEIEAGRLTLDAFVAKQANWIAQLVAHYRSHTLAIAVETSPSCPMCNASMLRRKGKSGPFWSCSCYP
DCKGTVSISKDTRRS

Sequences:

>Translated_655_residues
MRLFLCEKPSQGRDIAKVLGATRRGDGCLIGTDSTVTWCIGHLLETAPPEAYGAQYKSWSLDHLPIIPAQWQVEVKSKTA
AQFKIIKRLLSEASSVVIATDADREGEMIARELLELCRYRGPVLRLWLSALNEASIRKALSSLKSGQVTFPLYHSALARS
RADWLIGMNLSRLFTLLGRRAGYDGVLSVGRVQTPTLRLVVDRDRAIASFVPVPYWTVDVHLSSMGQRFIASWLPPRSGR
DEAGRCLQQALAHHAVQTITGEKSATVLSLQTEQLREPPPLPFDLSTLQEVCSRKLGLGVQETLNIAQALYETHKATTYP
RSDCRYLPESMFNEAPAVLDALLKTDPALRPAFGSLDRSLRARVWNNAKVTAHHGIIPTTEPANLVRMSEQERQVYELIR
SHYLAQFLPHHEFDRTQVELECSKERLTAVGKQILIQGWKGLLTENTEDDEPSQKHQVLPVLQQGTQCAVNDVELKSMRT
VAPKPLTEGDLIKAMKNVAKLVNDPRLKQKLRDTTGIGTEATRAGIIKGLVDRGYLLKKKRALMASAAAHTLIEAAPTAV
ADPGMTAIWEQALDEIEAGRLTLDAFVAKQANWIAQLVAHYRSHTLAIAVETSPSCPMCNASMLRRKGKSGPFWSCSCYP
DCKGTVSISKDTRRS
>Mature_655_residues
MRLFLCEKPSQGRDIAKVLGATRRGDGCLIGTDSTVTWCIGHLLETAPPEAYGAQYKSWSLDHLPIIPAQWQVEVKSKTA
AQFKIIKRLLSEASSVVIATDADREGEMIARELLELCRYRGPVLRLWLSALNEASIRKALSSLKSGQVTFPLYHSALARS
RADWLIGMNLSRLFTLLGRRAGYDGVLSVGRVQTPTLRLVVDRDRAIASFVPVPYWTVDVHLSSMGQRFIASWLPPRSGR
DEAGRCLQQALAHHAVQTITGEKSATVLSLQTEQLREPPPLPFDLSTLQEVCSRKLGLGVQETLNIAQALYETHKATTYP
RSDCRYLPESMFNEAPAVLDALLKTDPALRPAFGSLDRSLRARVWNNAKVTAHHGIIPTTEPANLVRMSEQERQVYELIR
SHYLAQFLPHHEFDRTQVELECSKERLTAVGKQILIQGWKGLLTENTEDDEPSQKHQVLPVLQQGTQCAVNDVELKSMRT
VAPKPLTEGDLIKAMKNVAKLVNDPRLKQKLRDTTGIGTEATRAGIIKGLVDRGYLLKKKRALMASAAAHTLIEAAPTAV
ADPGMTAIWEQALDEIEAGRLTLDAFVAKQANWIAQLVAHYRSHTLAIAVETSPSCPMCNASMLRRKGKSGPFWSCSCYP
DCKGTVSISKDTRRS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=707, Percent_Identity=25.7425742574257, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI4507635, Length=647, Percent_Identity=24.4204018547141, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1788061, Length=608, Percent_Identity=41.9407894736842, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1787529, Length=597, Percent_Identity=25.7956448911223, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32563869, Length=571, Percent_Identity=25.7443082311734, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=679, Percent_Identity=23.2695139911635, Blast_Score=113, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=504, Percent_Identity=27.3809523809524, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24585251, Length=661, Percent_Identity=26.7776096822995, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24640096, Length=590, Percent_Identity=25.9322033898305, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72558; Mature: 72558

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFLCEKPSQGRDIAKVLGATRRGDGCLIGTDSTVTWCIGHLLETAPPEAYGAQYKSWS
CEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHCCCCCCCC
LDHLPIIPAQWQVEVKSKTAAQFKIIKRLLSEASSVVIATDADREGEMIARELLELCRYR
CCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCC
GPVLRLWLSALNEASIRKALSSLKSGQVTFPLYHSALARSRADWLIGMNLSRLFTLLGRR
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCEEECCCHHHHHHHHHHC
AGYDGVLSVGRVQTPTLRLVVDRDRAIASFVPVPYWTVDVHLSSMGQRFIASWLPPRSGR
CCCCHHHCCCCCCCCEEEEEEECCCHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCCCC
DEAGRCLQQALAHHAVQTITGEKSATVLSLQTEQLREPPPLPFDLSTLQEVCSRKLGLGV
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHCCCH
QETLNIAQALYETHKATTYPRSDCRYLPESMFNEAPAVLDALLKTDPALRPAFGSLDRSL
HHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHH
RARVWNNAKVTAHHGIIPTTEPANLVRMSEQERQVYELIRSHYLAQFLPHHEFDRTQVEL
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
ECSKERLTAVGKQILIQGWKGLLTENTEDDEPSQKHQVLPVLQQGTQCAVNDVELKSMRT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VAPKPLTEGDLIKAMKNVAKLVNDPRLKQKLRDTTGIGTEATRAGIIKGLVDRGYLLKKK
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHH
RALMASAAAHTLIEAAPTAVADPGMTAIWEQALDEIEAGRLTLDAFVAKQANWIAQLVAH
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YRSHTLAIAVETSPSCPMCNASMLRRKGKSGPFWSCSCYPDCKGTVSISKDTRRS
HCCCEEEEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MRLFLCEKPSQGRDIAKVLGATRRGDGCLIGTDSTVTWCIGHLLETAPPEAYGAQYKSWS
CEEEEECCCCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHCCCCCCCC
LDHLPIIPAQWQVEVKSKTAAQFKIIKRLLSEASSVVIATDADREGEMIARELLELCRYR
CCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCC
GPVLRLWLSALNEASIRKALSSLKSGQVTFPLYHSALARSRADWLIGMNLSRLFTLLGRR
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCEEECCCHHHHHHHHHHC
AGYDGVLSVGRVQTPTLRLVVDRDRAIASFVPVPYWTVDVHLSSMGQRFIASWLPPRSGR
CCCCHHHCCCCCCCCEEEEEEECCCHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCCCC
DEAGRCLQQALAHHAVQTITGEKSATVLSLQTEQLREPPPLPFDLSTLQEVCSRKLGLGV
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHCCCH
QETLNIAQALYETHKATTYPRSDCRYLPESMFNEAPAVLDALLKTDPALRPAFGSLDRSL
HHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHH
RARVWNNAKVTAHHGIIPTTEPANLVRMSEQERQVYELIRSHYLAQFLPHHEFDRTQVEL
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
ECSKERLTAVGKQILIQGWKGLLTENTEDDEPSQKHQVLPVLQQGTQCAVNDVELKSMRT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VAPKPLTEGDLIKAMKNVAKLVNDPRLKQKLRDTTGIGTEATRAGIIKGLVDRGYLLKKK
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHH
RALMASAAAHTLIEAAPTAVADPGMTAIWEQALDEIEAGRLTLDAFVAKQANWIAQLVAH
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YRSHTLAIAVETSPSCPMCNASMLRRKGKSGPFWSCSCYPDCKGTVSISKDTRRS
HCCCEEEEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]