BacMap

Definition	Pseudomonas fluorescens Pf0-1 chromosome, complete genome.
Accession	NC_007492
Length	6,438,405

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The map label for this gene is benD [H]

Identifier: 77459190

GI number: 77459190

Start: 3402571

End: 3403332

Strand: Reverse

Name: benD [H]

Synonym: Pfl01_2965

Alternate gene names: 77459190

Gene position: 3403332-3402571 (Counterclockwise)

Preceding gene: 77459191

Following gene: 77459189

Centisome position: 52.86

GC content: 59.45

Gene sequence:

>762_bases
ATGAAAAACAGATTCGAAAACAAGGTTGCCTTGGTGACTGGCGCGGCTCAAGGCATCGGTCGACGCGTGTGCGAGCAACT
GCGGGCCGAGGGCGCGCGGGTTGTAGCGGTGGATCGATCGGAGCTGGTGCATGAGCTCAAACAAGACGACGTATTGACCC
TCGTCGCCGATCTGGAAATGCATACCGAATGCATGTCGGTGATGGCAGCCGCCGTCGAGGCTTTCGGCAGCCTGCACATA
TTGATCAACAACGTGGGCGGGACTATCTGGACCAAACCGTTCGAGCGTTACGAGGCCGCGCAAATCGAGTCGGAAGTGCG
TCGGTCGTTGTTCCCGACCCTCTGGTGCTGTCACGCGGCGTTGCCCTACATGCTCGAACAAGGGCACGGCGCGATCGTCA
ACGTCTCGTCAATCGCGACACGGGGCGTCAACCGTGTTCCGTATGGCGCAGCCAAAGGTGGCGTTAACGCCTTGACCGCC
TGCTTGGCGTTCGAAAATGCCGAACGTGGTATCCGGGTCAATGCGATCGCGACCGGGGGCACCGAGGCGCCACCGCGACG
TATCCCCCGCAACACCGCGCAGCCAACCGAAGAAGAGAAGGTTTGGTATCAGCAAATCGTCGACCAGACGATTCAAAGCA
GTTTGATGAAGCGTTACGGAACGATTGACGAACAGGTCGCCGCGATCTTGTTCCTGGCCTCGGATGACGCGTCCTATATC
ACTGGCGTAACCCTGCCAGTCAGTGGCGGTGACCTCGGCTGA

Upstream 100 bases:

>100_bases
ACATCCGCGAGCAAGGGATTAACCCTGCCAATTTCTATTTCGAGAAGTTCGCGCCTGCCGCGGCCTGAATCGTTACGCAA
AGACTCCGTTCGAGGTTGTC

Downstream 100 bases:

>100_bases
CCAGGAAAACTTGTCGCATCAGGCGCGACAGATTGCATCGCCGATGTAGTTCCCAAAATAAAAACAAGAGTAACGACGCC
ATGCGAGTAATCGACGTTAA

Product: 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase

Products: NA

Alternate protein names: 2-hydro-1,2-dihydroxybenzoate dehydrogenase; DHB dehydrogenase; Cis-1,2-dihydroxy-3,4-cyclohexadiene-1-carboxylate dehydrogenase; Cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKNRFENKVALVTGAAQGIGRRVCEQLRAEGARVVAVDRSELVHELKQDDVLTLVADLEMHTECMSVMAAAVEAFGSLHI
LINNVGGTIWTKPFERYEAAQIESEVRRSLFPTLWCCHAALPYMLEQGHGAIVNVSSIATRGVNRVPYGAAKGGVNALTA
CLAFENAERGIRVNAIATGGTEAPPRRIPRNTAQPTEEEKVWYQQIVDQTIQSSLMKRYGTIDEQVAAILFLASDDASYI
TGVTLPVSGGDLG

Sequences:

>Translated_253_residues
MKNRFENKVALVTGAAQGIGRRVCEQLRAEGARVVAVDRSELVHELKQDDVLTLVADLEMHTECMSVMAAAVEAFGSLHI
LINNVGGTIWTKPFERYEAAQIESEVRRSLFPTLWCCHAALPYMLEQGHGAIVNVSSIATRGVNRVPYGAAKGGVNALTA
CLAFENAERGIRVNAIATGGTEAPPRRIPRNTAQPTEEEKVWYQQIVDQTIQSSLMKRYGTIDEQVAAILFLASDDASYI
TGVTLPVSGGDLG
>Mature_253_residues
MKNRFENKVALVTGAAQGIGRRVCEQLRAEGARVVAVDRSELVHELKQDDVLTLVADLEMHTECMSVMAAAVEAFGSLHI
LINNVGGTIWTKPFERYEAAQIESEVRRSLFPTLWCCHAALPYMLEQGHGAIVNVSSIATRGVNRVPYGAAKGGVNALTA
CLAFENAERGIRVNAIATGGTEAPPRRIPRNTAQPTEEEKVWYQQIVDQTIQSSLMKRYGTIDEQVAAILFLASDDASYI
TGVTLPVSGGDLG

Specific function: Degradation of 2-hydro-1,2-dihydroxy benzoate (DHB) to catechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=30.5019305019305, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI32483357, Length=271, Percent_Identity=30.2583025830258, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI7705925, Length=250, Percent_Identity=30.8, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI59889578, Length=231, Percent_Identity=30.3030303030303, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI31542939, Length=250, Percent_Identity=30.8, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI126723750, Length=273, Percent_Identity=27.1062271062271, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=27.2, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI10190704, Length=268, Percent_Identity=28.3582089552239, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI66933014, Length=252, Percent_Identity=29.7619047619048, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787335, Length=252, Percent_Identity=32.1428571428571, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789208, Length=254, Percent_Identity=29.1338582677165, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367175, Length=265, Percent_Identity=30.188679245283, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787905, Length=258, Percent_Identity=28.6821705426357, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=27.734375, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790717, Length=254, Percent_Identity=26.3779527559055, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789378, Length=261, Percent_Identity=28.3524904214559, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789057, Length=264, Percent_Identity=25.3787878787879, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788459, Length=260, Percent_Identity=25.3846153846154, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=262, Percent_Identity=32.4427480916031, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17561402, Length=270, Percent_Identity=31.1111111111111, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71994604, Length=262, Percent_Identity=31.2977099236641, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71994600, Length=263, Percent_Identity=30.7984790874525, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560676, Length=253, Percent_Identity=29.2490118577075, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=30.3370786516854, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560332, Length=274, Percent_Identity=27.7372262773723, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531453, Length=195, Percent_Identity=32.3076923076923, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=269, Percent_Identity=28.996282527881, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=278, Percent_Identity=28.4172661870504, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=260, Percent_Identity=26.5384615384615, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=27.9527559055118, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=277, Percent_Identity=27.4368231046931, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=272, Percent_Identity=30.1470588235294, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=269, Percent_Identity=27.1375464684015, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=189, Percent_Identity=29.1005291005291, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=190, Percent_Identity=26.8421052631579, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=195, Percent_Identity=29.2307692307692, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=265, Percent_Identity=26.4150943396226, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=196, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=193, Percent_Identity=30.0518134715026, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=266, Percent_Identity=26.6917293233083, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=194, Percent_Identity=29.3814432989691, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=268, Percent_Identity=26.1194029850746, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=261, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=189, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=7e-11,
Organism=Drosophila melanogaster, GI28571526, Length=264, Percent_Identity=31.0606060606061, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24644339, Length=264, Percent_Identity=31.0606060606061, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI23397609, Length=259, Percent_Identity=31.2741312741313, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357041, Length=262, Percent_Identity=29.3893129770992, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24639444, Length=253, Percent_Identity=30.8300395256917, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24644337, Length=260, Percent_Identity=32.3076923076923, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21355319, Length=277, Percent_Identity=28.8808664259928, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24643142, Length=259, Percent_Identity=27.4131274131274, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364892, Length=168, Percent_Identity=30.3571428571429, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.25 [H]

Molecular weight: Translated: 27427; Mature: 27427

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRFENKVALVTGAAQGIGRRVCEQLRAEGARVVAVDRSELVHELKQDDVLTLVADLEM
CCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCHHHHHHHHHH
HTECMSVMAAAVEAFGSLHILINNVGGTIWTKPFERYEAAQIESEVRRSLFPTLWCCHAA
HHHHHHHHHHHHHHHCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPYMLEQGHGAIVNVSSIATRGVNRVPYGAAKGGVNALTACLAFENAERGIRVNAIATGG
HHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCC
TEAPPRRIPRNTAQPTEEEKVWYQQIVDQTIQSSLMKRYGTIDEQVAAILFLASDDASYI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEECCCCCEE
TGVTLPVSGGDLG
EEEEEECCCCCCC
>Mature Secondary Structure
MKNRFENKVALVTGAAQGIGRRVCEQLRAEGARVVAVDRSELVHELKQDDVLTLVADLEM
CCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCHHHHHHHHHH
HTECMSVMAAAVEAFGSLHILINNVGGTIWTKPFERYEAAQIESEVRRSLFPTLWCCHAA
HHHHHHHHHHHHHHHCCEEEEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPYMLEQGHGAIVNVSSIATRGVNRVPYGAAKGGVNALTACLAFENAERGIRVNAIATGG
HHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCC
TEAPPRRIPRNTAQPTEEEKVWYQQIVDQTIQSSLMKRYGTIDEQVAAILFLASDDASYI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEECCCCCEE
TGVTLPVSGGDLG
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1740120 [H]