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The map label for this gene is catB [H]

Identifier: 77459188

GI number: 77459188

Start: 3399964

End: 3401085

Strand: Reverse

Name: catB [H]

Synonym: Pfl01_2963

Alternate gene names: 77459188

Gene position: 3401085-3399964 (Counterclockwise)

Preceding gene: 77459189

Following gene: 77459187

Centisome position: 52.82

GC content: 57.58

Gene sequence:

>1122_bases
ATGAGCCAAGTCCTGATCCACAGCCTGTCGGCGATCATCGTCGACCTGCCGACTATTCGTCCGCACAAGCTCGCCATGCA
CACGATGCAAAAGCAAACCTTGGTCATTCTGCGGCTTCAATGCAGTGACGGCATCGAAGGTATCGGCGAAGCCACCACCA
TCGGCGGTTTGTCCTATGGCTATGAAAGCCCGGAGAGCATCAAGGCCAACGTTGACGCTCACTTGGCGCCCGCGCTGGTA
GGCATGGCAGCGGACAACATTAATGCCGCGATGCAAAAACTCGACAAGATTGCCAAGGGCAATACCTTTGCCAAATCCGG
TATCGAAAGCGCTCTACTCGATGCCCAGGGCAAACGAGTTGGAATGGCCGTCAGCGAGCTGCTTGGCGGTCGGGTACGCG
ACAGTCTGGAAGTCGCCTGGACGCTTGCCAGCGGCGATACCGCCCGGGACATTGCCGAGGCCGAGCAGATGTTGGAGCTG
CGCCGTCATCGCATCTTCAAACTGAAGATCGGCGCCAATCCTTTGGAGCAGGATCTCAAGCATGTCGTTGCGATCAAAAA
GGCCCTTGGCGATCGTGCCAGTGTAAGGGTCGACGTCAACCAATATTGGGACGAGTCTCAGGCAATACGTGGCTGCCAGG
TGCTTGGCGAGAACGGCATTGATCTGATCGAGCAACCCATCTCACGGGTTAATCGATCAGGCCAGGTTCGGCTGAACCAG
CGAGGCCCGGCACCCATCATGGCCGATGAGTCAATTGAAAGCGTCGAAGACGCGTTCAGCCTGGCGGCGGATGGCGCAGC
CAGCGTCTTCGCGCTAAAAATCGCAAAAAATGGTGGCCCTCGCGCTGTGCTGCGTACGGCACAGATTGCCGAAGCGGCGG
GCATCGCCCTGTACGGGGGGACGATGCTGGAAGGTGCCATCGGCACTTTAGCTTCAGCTCATGCATTCCTCACGCTTAAA
CACCTGACCTGGGACACAGAGCTGTTTGGTCCGCTCTTGTTGACCGAAGACATCGTGGTCGAGCGACCACAGTACCGTGA
CTTCCAGCTTCTTGTTCCATCCACGCCAGGCTTGGGATTGGCATTGGATGAAGATCGCCTGGCTCATTTTCGCCGTTCCT
GA

Upstream 100 bases:

>100_bases
GCCGATTCGAGTGTTTTGATGGCCGCCAAACCGTAACCAATCTTGCCTTGTTCCTTGAGCGGAACCAGGTGTCTCTCAGT
TCACGTATCGGAATATTGTC

Downstream 100 bases:

>100_bases
ACCCGCAACCCTATAAGGAGAGCACCATGCTATTCCACGTCAAGATGACCGTGAACCTCCCACTCGATATGGATGCGAGC
AGGGCAGCGCAGCTCAAAGC

Product: muconate cycloisomerase

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I; MLE; Muconate cycloisomerase I [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSQVLIHSLSAIIVDLPTIRPHKLAMHTMQKQTLVILRLQCSDGIEGIGEATTIGGLSYGYESPESIKANVDAHLAPALV
GMAADNINAAMQKLDKIAKGNTFAKSGIESALLDAQGKRVGMAVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQMLEL
RRHRIFKLKIGANPLEQDLKHVVAIKKALGDRASVRVDVNQYWDESQAIRGCQVLGENGIDLIEQPISRVNRSGQVRLNQ
RGPAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIALYGGTMLEGAIGTLASAHAFLTLK
HLTWDTELFGPLLLTEDIVVERPQYRDFQLLVPSTPGLGLALDEDRLAHFRRS

Sequences:

>Translated_373_residues
MSQVLIHSLSAIIVDLPTIRPHKLAMHTMQKQTLVILRLQCSDGIEGIGEATTIGGLSYGYESPESIKANVDAHLAPALV
GMAADNINAAMQKLDKIAKGNTFAKSGIESALLDAQGKRVGMAVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQMLEL
RRHRIFKLKIGANPLEQDLKHVVAIKKALGDRASVRVDVNQYWDESQAIRGCQVLGENGIDLIEQPISRVNRSGQVRLNQ
RGPAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIALYGGTMLEGAIGTLASAHAFLTLK
HLTWDTELFGPLLLTEDIVVERPQYRDFQLLVPSTPGLGLALDEDRLAHFRRS
>Mature_372_residues
SQVLIHSLSAIIVDLPTIRPHKLAMHTMQKQTLVILRLQCSDGIEGIGEATTIGGLSYGYESPESIKANVDAHLAPALVG
MAADNINAAMQKLDKIAKGNTFAKSGIESALLDAQGKRVGMAVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQMLELR
RHRIFKLKIGANPLEQDLKHVVAIKKALGDRASVRVDVNQYWDESQAIRGCQVLGENGIDLIEQPISRVNRSGQVRLNQR
GPAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIALYGGTMLEGAIGTLASAHAFLTLKH
LTWDTELFGPLLLTEDIVVERPQYRDFQLLVPSTPGLGLALDEDRLAHFRRS

Specific function: Catalyzes a syn cycloisomerization [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=260, Percent_Identity=27.3076923076923, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 40133; Mature: 40001

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVLIHSLSAIIVDLPTIRPHKLAMHTMQKQTLVILRLQCSDGIEGIGEATTIGGLSYG
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEECCCCCC
YESPESIKANVDAHLAPALVGMAADNINAAMQKLDKIAKGNTFAKSGIESALLDAQGKRV
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHH
GMAVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQMLELRRHRIFKLKIGANPLEQDLK
HHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHH
HVVAIKKALGDRASVRVDVNQYWDESQAIRGCQVLGENGIDLIEQPISRVNRSGQVRLNQ
HHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECC
RGPAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIALYGG
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEECC
TMLEGAIGTLASAHAFLTLKHLTWDTELFGPLLLTEDIVVERPQYRDFQLLVPSTPGLGL
HHHHHHHHHHHHHHHEEEEEEEECCHHHHCHHHCCCHHHHCCCCCCCEEEEECCCCCCCE
ALDEDRLAHFRRS
EECHHHHHHHHCC
>Mature Secondary Structure 
SQVLIHSLSAIIVDLPTIRPHKLAMHTMQKQTLVILRLQCSDGIEGIGEATTIGGLSYG
CHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEECCCCCC
YESPESIKANVDAHLAPALVGMAADNINAAMQKLDKIAKGNTFAKSGIESALLDAQGKRV
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHH
GMAVSELLGGRVRDSLEVAWTLASGDTARDIAEAEQMLELRRHRIFKLKIGANPLEQDLK
HHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHH
HVVAIKKALGDRASVRVDVNQYWDESQAIRGCQVLGENGIDLIEQPISRVNRSGQVRLNQ
HHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECC
RGPAPIMADESIESVEDAFSLAADGAASVFALKIAKNGGPRAVLRTAQIAEAAGIALYGG
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEECC
TMLEGAIGTLASAHAFLTLKHLTWDTELFGPLLLTEDIVVERPQYRDFQLLVPSTPGLGL
HHHHHHHHHHHHHHHEEEEEEEECCHHHHCHHHCCCHHHHCCCCCCCEEEEECCCCCCCE
ALDEDRLAHFRRS
EECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3609743; 2449420; 2215699; 3612800; 7500361 [H]