Click here to switch to the map view.

The map label for this gene is gloA [H]

Identifier: 77459148

GI number: 77459148

Start: 3368558

End: 3369079

Strand: Direct

Name: gloA [H]

Synonym: Pfl01_2923

Alternate gene names: 77459148

Gene position: 3368558-3369079 (Clockwise)

Preceding gene: 77459146

Following gene: 77459152

Centisome position: 52.32

GC content: 59.58

Gene sequence:

>522_bases
ATGAGCCTGCAAGAACTGAACACCTTCCCTGGCGTCACCGCCATCCCGGACAGCGCAACCCGTCACTTCGTGTTCAACCA
CACCATGCTGCGCGTCAAAGACATCACCAAGTCGCTGGATTTCTACACCCGCGTACTGGGTTTCTCGCTGGTAGAGAAGC
GTGATTTCCCGGAAGCGGAATTCAGCCTGTACTTCCTCGCCTTGGTGGACAAATCGCAGATCCCGGCCGACGCCGCTGCT
CGCACCGAGTGGATGAAGTCGATTCCCGGCATTCTGGAACTGACCCACAACCATGGCACCGAGAACGACGCAGACTTCGC
CTACCACAACGGCAACACCGACCCGCGCGGTTTCGGCCACATCTGCATTTCGGTACCGGACATCGTCGCTGCCTGCGCGC
GCTTTGAAGAGCTGGGCTGCGACTTCCAGAAGCGTCTGACCGATGGCCGCATGAAGAGCCTGGCGTTCATCAAGGATCCG
GATGGCTACTGGGTCGAGATCATCCAGCCGGCGCCGCTGTAA

Upstream 100 bases:

>100_bases
GCCCGCTTGCAGGGCAAGCCCGCGCTTTCGGTTTGGCGATGCCGCAAGGCATGCGTATCATTGCGCCCATTCACTCGCCC
CAATTTGTTACGGAGGGCCC

Downstream 100 bases:

>100_bases
AGCTGAACGCAGGTAGAAAGCATCACGCAAAGAAAAACCCCCATCATCACTGATGGGGGTTTTCTTTTTCAGACCCTGGA
TTTACGCCGGAGCCGACGTG

Product: glyoxalase I

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSLQELNTFPGVTAIPDSATRHFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAEFSLYFLALVDKSQIPADAAA
RTEWMKSIPGILELTHNHGTENDADFAYHNGNTDPRGFGHICISVPDIVAACARFEELGCDFQKRLTDGRMKSLAFIKDP
DGYWVEIIQPAPL

Sequences:

>Translated_173_residues
MSLQELNTFPGVTAIPDSATRHFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAEFSLYFLALVDKSQIPADAAA
RTEWMKSIPGILELTHNHGTENDADFAYHNGNTDPRGFGHICISVPDIVAACARFEELGCDFQKRLTDGRMKSLAFIKDP
DGYWVEIIQPAPL
>Mature_172_residues
SLQELNTFPGVTAIPDSATRHFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAEFSLYFLALVDKSQIPADAAAR
TEWMKSIPGILELTHNHGTENDADFAYHNGNTDPRGFGHICISVPDIVAACARFEELGCDFQKRLTDGRMKSLAFIKDPD
GYWVEIIQPAPL

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=154, Percent_Identity=60.3896103896104, Blast_Score=202, Evalue=2e-52,
Organism=Escherichia coli, GI1787940, Length=145, Percent_Identity=31.7241379310345, Blast_Score=81, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323639, Length=147, Percent_Identity=48.9795918367347, Blast_Score=146, Evalue=2e-36,
Organism=Drosophila melanogaster, GI20129743, Length=152, Percent_Identity=61.8421052631579, Blast_Score=200, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 19482; Mature: 19351

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQELNTFPGVTAIPDSATRHFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAE
CCHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FSLYFLALVDKSQIPADAAARTEWMKSIPGILELTHNHGTENDADFAYHNGNTDPRGFGH
HEEEEEEEECCCCCCCHHHHHHHHHHHCCHHEEEECCCCCCCCCCEEEECCCCCCCCCCE
ICISVPDIVAACARFEELGCDFQKRLTDGRMKSLAFIKDPDGYWVEIIQPAPL
EEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHHEEEEECCCCCEEEEECCCCC
>Mature Secondary Structure 
SLQELNTFPGVTAIPDSATRHFVFNHTMLRVKDITKSLDFYTRVLGFSLVEKRDFPEAE
CHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
FSLYFLALVDKSQIPADAAARTEWMKSIPGILELTHNHGTENDADFAYHNGNTDPRGFGH
HEEEEEEEECCCCCCCHHHHHHHHHHHCCHHEEEECCCCCCCCCCEEEECCCCCCCCCCE
ICISVPDIVAACARFEELGCDFQKRLTDGRMKSLAFIKDPDGYWVEIIQPAPL
EEEEHHHHHHHHHHHHHHCCCHHHHHCCCHHHHEEEEECCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959071 [H]