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The map label for this gene is ghrB [H]

Identifier: 77459129

GI number: 77459129

Start: 3348396

End: 3349364

Strand: Direct

Name: ghrB [H]

Synonym: Pfl01_2904

Alternate gene names: 77459129

Gene position: 3348396-3349364 (Clockwise)

Preceding gene: 77459128

Following gene: 77459131

Centisome position: 52.01

GC content: 61.61

Gene sequence:

>969_bases
ATGAAAAAGCAGGTTGTGTTGTACAAGAAACTGTCGCCGGCGCTGATGGCGCGCCTTGAGGAACAGGTCGATGTGACGCT
GATCGACAGCCTCGACGCCGACGGCCTGATGAAACTGCGCGACGCCCTCCCCGGTGCCCACGGCCTGCTCGGTGCGAGCC
TGAAACTGGACGCGGCGCTGCTCGATCTGGCGCCGAACCTTGAAGCGATTTCCAGCGTCTCGGTGGGTGTCGACAACTAC
GACATCGACTACCTGACCCAACGCAAGGTCATGCTGACCAACACCCCGGACGTGCTCACCGAAACCACCGCCGACACCGG
TTTCGCACTGATCCTGGCCACCGCCCGGCGCGTGGTTGAGCTGGCGAACATGGTGCGCGGCGGCCACTGGCACCGCAGCA
TCGGCCCGGCGCATTTCGGTACCGATGTGCACGGCAAGACCCTGGGCATCATCGGTATGGGGCGGATCGGTGAGGCGCTG
GCCCAGCGTGGACATTTCGGGTTCGGGATGCCGGTGATCTATCACAGCCAGTCGCGCAAACCGGCGGTTGAAGAACGTTT
CGATGCACAGTACCGCAGCCTTGAAGACTTGTTGCAGCAGGCGGATTTCATCTGCCTGACATTGCCGCTGACGGCGCAGA
CTGAAGGTCTGATTGGCGCCGAACAGTTTGCGTTGATGCGACCGGAGAGCATCTTCATCAACATTTCACGAGGCAAAGTT
GTGGATGAGGCCGCGCTGATCGACACCTTGCGCCATAACCGGATTCGCGCGGCGGGGCTGGATGTGTTCGAGCGTGAACC
GTTGAATCATGACTCGCCGTTGTTGCAGTTGAACAACGTGGTGGCGACGCCGCACATGGGCTCGGCAACCCATGAAACGC
GCGAAGCGATGGCGCGGTGTGCGGTGGAGAATCTGTTGGCGGCGCTGGCGGGACAAAGGCCCGCCAATCTGGTGAATCCC
CCACTCTGA

Upstream 100 bases:

>100_bases
CATTTTTCTCAAGCCCGGCGCGAGCGACCGGGTGACGGCCAAGCGCGTCGCACCGCGTCCGCTGCCGGCCCATTCCTGAA
TTGATAGAGAGATGACTGCG

Downstream 100 bases:

>100_bases
TCTTGGTCAGGCGCTGCGCACCTGGAGAAGCTGCGCAGCGCACAGCGCAATCCGCGAACACGCATCCGCCAGCTTCACCC
GATCCACCACCAACCCGATG

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKKQVVLYKKLSPALMARLEEQVDVTLIDSLDADGLMKLRDALPGAHGLLGASLKLDAALLDLAPNLEAISSVSVGVDNY
DIDYLTQRKVMLTNTPDVLTETTADTGFALILATARRVVELANMVRGGHWHRSIGPAHFGTDVHGKTLGIIGMGRIGEAL
AQRGHFGFGMPVIYHSQSRKPAVEERFDAQYRSLEDLLQQADFICLTLPLTAQTEGLIGAEQFALMRPESIFINISRGKV
VDEAALIDTLRHNRIRAAGLDVFEREPLNHDSPLLQLNNVVATPHMGSATHETREAMARCAVENLLAALAGQRPANLVNP
PL

Sequences:

>Translated_322_residues
MKKQVVLYKKLSPALMARLEEQVDVTLIDSLDADGLMKLRDALPGAHGLLGASLKLDAALLDLAPNLEAISSVSVGVDNY
DIDYLTQRKVMLTNTPDVLTETTADTGFALILATARRVVELANMVRGGHWHRSIGPAHFGTDVHGKTLGIIGMGRIGEAL
AQRGHFGFGMPVIYHSQSRKPAVEERFDAQYRSLEDLLQQADFICLTLPLTAQTEGLIGAEQFALMRPESIFINISRGKV
VDEAALIDTLRHNRIRAAGLDVFEREPLNHDSPLLQLNNVVATPHMGSATHETREAMARCAVENLLAALAGQRPANLVNP
PL
>Mature_322_residues
MKKQVVLYKKLSPALMARLEEQVDVTLIDSLDADGLMKLRDALPGAHGLLGASLKLDAALLDLAPNLEAISSVSVGVDNY
DIDYLTQRKVMLTNTPDVLTETTADTGFALILATARRVVELANMVRGGHWHRSIGPAHFGTDVHGKTLGIIGMGRIGEAL
AQRGHFGFGMPVIYHSQSRKPAVEERFDAQYRSLEDLLQQADFICLTLPLTAQTEGLIGAEQFALMRPESIFINISRGKV
VDEAALIDTLRHNRIRAAGLDVFEREPLNHDSPLLQLNNVVATPHMGSATHETREAMARCAVENLLAALAGQRPANLVNP
PL

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=293, Percent_Identity=40.9556313993174, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI23308577, Length=252, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI145580578, Length=259, Percent_Identity=32.4324324324324, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI4557499, Length=259, Percent_Identity=32.4324324324324, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI145580575, Length=259, Percent_Identity=32.4324324324324, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI4557497, Length=259, Percent_Identity=32.8185328185328, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI61743967, Length=259, Percent_Identity=32.8185328185328, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI87082289, Length=320, Percent_Identity=56.25, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789279, Length=263, Percent_Identity=32.6996197718631, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787645, Length=254, Percent_Identity=25.9842519685039, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081824, Length=282, Percent_Identity=28.3687943262411, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=263, Percent_Identity=32.6996197718631, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25147481, Length=318, Percent_Identity=24.2138364779874, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=248, Percent_Identity=37.0967741935484, Blast_Score=159, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6320925, Length=255, Percent_Identity=32.5490196078431, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=282, Percent_Identity=30.4964539007092, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321253, Length=281, Percent_Identity=28.1138790035587, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325144, Length=180, Percent_Identity=31.1111111111111, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324964, Length=240, Percent_Identity=23.75, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28574286, Length=314, Percent_Identity=35.6687898089172, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45552429, Length=301, Percent_Identity=36.2126245847176, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45551003, Length=301, Percent_Identity=36.2126245847176, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28574284, Length=301, Percent_Identity=36.2126245847176, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24585514, Length=301, Percent_Identity=36.2126245847176, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28574282, Length=301, Percent_Identity=36.2126245847176, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI28571528, Length=261, Percent_Identity=40.2298850574713, Blast_Score=174, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24585516, Length=278, Percent_Identity=34.1726618705036, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24646446, Length=261, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646448, Length=261, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646452, Length=261, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646450, Length=261, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921140, Length=253, Percent_Identity=32.4110671936759, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI62472511, Length=261, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35045; Mature: 35045

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQVVLYKKLSPALMARLEEQVDVTLIDSLDADGLMKLRDALPGAHGLLGASLKLDAAL
CCCCEEEHHHCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCHHCCCCHHHHHHH
LDLAPNLEAISSVSVGVDNYDIDYLTQRKVMLTNTPDVLTETTADTGFALILATARRVVE
HHHCCCHHHHHHEECCCCCCCCCHHCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHH
LANMVRGGHWHRSIGPAHFGTDVHGKTLGIIGMGRIGEALAQRGHFGFGMPVIYHSQSRK
HHHHHCCCCCCCCCCCHHCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCEEEECCCCC
PAVEERFDAQYRSLEDLLQQADFICLTLPLTAQTEGLIGAEQFALMRPESIFINISRGKV
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHEECCCEEEEEECCCCC
VDEAALIDTLRHNRIRAAGLDVFEREPLNHDSPLLQLNNVVATPHMGSATHETREAMARC
HHHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCCEEEECCEEECCCCCCCHHHHHHHHHHH
AVENLLAALAGQRPANLVNPPL
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKQVVLYKKLSPALMARLEEQVDVTLIDSLDADGLMKLRDALPGAHGLLGASLKLDAAL
CCCCEEEHHHCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCCCHHCCCCHHHHHHH
LDLAPNLEAISSVSVGVDNYDIDYLTQRKVMLTNTPDVLTETTADTGFALILATARRVVE
HHHCCCHHHHHHEECCCCCCCCCHHCCCEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHH
LANMVRGGHWHRSIGPAHFGTDVHGKTLGIIGMGRIGEALAQRGHFGFGMPVIYHSQSRK
HHHHHCCCCCCCCCCCHHCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCEEEECCCCC
PAVEERFDAQYRSLEDLLQQADFICLTLPLTAQTEGLIGAEQFALMRPESIFINISRGKV
CHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHEECCCEEEEEECCCCC
VDEAALIDTLRHNRIRAAGLDVFEREPLNHDSPLLQLNNVVATPHMGSATHETREAMARC
HHHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCCEEEECCEEECCCCCCCHHHHHHHHHHH
AVENLLAALAGQRPANLVNPPL
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA