BacMap

Definition	Pseudomonas fluorescens Pf0-1 chromosome, complete genome.
Accession	NC_007492
Length	6,438,405

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The map label for this gene is fadB1 [H]

Identifier: 77459091

GI number: 77459091

Start: 3306362

End: 3307135

Strand: Reverse

Name: fadB1 [H]

Synonym: Pfl01_2866

Alternate gene names: 77459091

Gene position: 3307135-3306362 (Counterclockwise)

Preceding gene: 77459092

Following gene: 77459076

Centisome position: 51.37

GC content: 62.4

Gene sequence:

>774_bases
ATGAGTTACGAAACGATTTTGCTGGAAACCCATGGCCGCGTCGGCCTGATTACCCTCAATCGCCCGCAGGCGCTGAATGC
CCTGAATGCGCAACTGGTCAGCGAAGTGAATCGCGCGCTCGATGGATTTGAAGCCGACGCGAACATCGGCTGCATCGTCA
TCACCGGCTCGACCAAGGCCTTCGCCGCCGGCGCCGACATCAAGGAAATGGCCGATCTGACCTACCCGCAGATCTACATG
GACGACCTGTTCAGCGACAGCGATCGCGTGGCCAACCGGCGCAAGCCGATCATCGCCGCGGTCAACGGTTTTGCCCTCGG
CGGCGGCTGTGAGCTGGCGCTGATGTGCGACTTCATCCTGGCCGGCGACAACGCCAAATTCGGCCAGCCGGAAATCAACC
TCGGCGTGCTGCCGGGCATGGGCGGCACCCAGCGCCTGACCCGCGCGGTGGGCAAGGCCAAGGCCATGGAAATGTGCCTG
AGCGGCCGCATGATCGACGCGGTGGAAGCCGAGCGCTGCGGCATCGTCGCGCGAATCGTGCCGAGTGCCGAGTTGCTGGA
TGAGGCGCTGAAAGTCGCCGCAGTGATCGCCAGCAAATCGCTGCCGATTACGATGATGGTCAAGGAAAGCGTCAACCGCG
CGTTTGAAGTGAATCTGACCGAAGGCGTGCGCTTCGAGCGCCGGGTGTTCCATGCGGCGTTTGCGACGCAGGATCAGAAG
GAAGGGATGGCGGCGTTTGTGGCCAAGCGTGCGCCTGAATTTCAGGGCAAGTAA

Upstream 100 bases:

>100_bases
AGCGCATCTACCGCGACGTGCGGGTCTGCCAGATCTACGAAGGCACCAGCGACATACAGCGCATGGTCATTTCGCGCAAT
CTTTGAGAAGGAATCTGCAC

Downstream 100 bases:

>100_bases
AACGGCAAAATCAAGAGCCCCTCACCCTAACCCTCTCCCAAAGGGAGAGGGGACTGACCGGGGGATATTGAAGAGATACG
CCGACTTGAAAGATCGCCTG

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSYETILLETHGRVGLITLNRPQALNALNAQLVSEVNRALDGFEADANIGCIVITGSTKAFAAGADIKEMADLTYPQIYM
DDLFSDSDRVANRRKPIIAAVNGFALGGGCELALMCDFILAGDNAKFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCL
SGRMIDAVEAERCGIVARIVPSAELLDEALKVAAVIASKSLPITMMVKESVNRAFEVNLTEGVRFERRVFHAAFATQDQK
EGMAAFVAKRAPEFQGK

Sequences:

>Translated_257_residues
MSYETILLETHGRVGLITLNRPQALNALNAQLVSEVNRALDGFEADANIGCIVITGSTKAFAAGADIKEMADLTYPQIYM
DDLFSDSDRVANRRKPIIAAVNGFALGGGCELALMCDFILAGDNAKFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCL
SGRMIDAVEAERCGIVARIVPSAELLDEALKVAAVIASKSLPITMMVKESVNRAFEVNLTEGVRFERRVFHAAFATQDQK
EGMAAFVAKRAPEFQGK
>Mature_256_residues
SYETILLETHGRVGLITLNRPQALNALNAQLVSEVNRALDGFEADANIGCIVITGSTKAFAAGADIKEMADLTYPQIYMD
DLFSDSDRVANRRKPIIAAVNGFALGGGCELALMCDFILAGDNAKFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCLS
GRMIDAVEAERCGIVARIVPSAELLDEALKVAAVIASKSLPITMMVKESVNRAFEVNLTEGVRFERRVFHAAFATQDQKE
GMAAFVAKRAPEFQGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=53.6964980544747, Blast_Score=278, Evalue=3e-75,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=34.4961240310078, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI31542718, Length=243, Percent_Identity=30.4526748971193, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=36.1256544502618, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI20127408, Length=218, Percent_Identity=34.4036697247706, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=31.7073170731707, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=32.8205128205128, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI37594471, Length=195, Percent_Identity=32.8205128205128, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=30.4347826086957, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI213417737, Length=228, Percent_Identity=25.4385964912281, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI157694516, Length=228, Percent_Identity=25.4385964912281, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI45643119, Length=217, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI260275230, Length=217, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI260274832, Length=217, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI261878539, Length=97, Percent_Identity=41.2371134020619, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4757968, Length=261, Percent_Identity=23.7547892720307, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI49355787, Length=261, Percent_Identity=23.7547892720307, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI25453481, Length=260, Percent_Identity=23.8461538461538, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI51538573, Length=260, Percent_Identity=23.8461538461538, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI157694520, Length=199, Percent_Identity=26.1306532663317, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=240, Percent_Identity=25.4166666666667, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=240, Percent_Identity=25.4166666666667, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=240, Percent_Identity=25.4166666666667, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4757966, Length=196, Percent_Identity=25.5102040816327, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI51538581, Length=196, Percent_Identity=25.5102040816327, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI62530384, Length=221, Percent_Identity=24.8868778280543, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=44.6153846153846, Blast_Score=213, Evalue=8e-57,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=36.5384615384615, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=34.9809885931559, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=33.4677419354839, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=30.5343511450382, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1790281, Length=178, Percent_Identity=32.5842696629214, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788682, Length=207, Percent_Identity=32.8502415458937, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=53.6964980544747, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=51.8672199170125, Blast_Score=256, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=29.5964125560538, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=220, Percent_Identity=32.7272727272727, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=244, Percent_Identity=30.327868852459, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=29.1666666666667, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=216, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=213, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=213, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144157, Length=191, Percent_Identity=29.3193717277487, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=191, Percent_Identity=29.3193717277487, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=32.579185520362, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=275, Percent_Identity=28, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=25.78125, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=258, Percent_Identity=27.906976744186, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25141363, Length=230, Percent_Identity=24.7826086956522, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=195, Percent_Identity=28.7179487179487, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=57.8125, Blast_Score=297, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=57.8125, Blast_Score=297, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=33.8645418326693, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=27.6923076923077, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=30.1204819277108, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21357171, Length=234, Percent_Identity=31.6239316239316, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24650670, Length=211, Percent_Identity=34.1232227488152, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28571730, Length=239, Percent_Identity=26.7782426778243, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571729, Length=239, Percent_Identity=26.7782426778243, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654903, Length=258, Percent_Identity=24.8062015503876, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583077, Length=175, Percent_Identity=34.8571428571429, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583079, Length=175, Percent_Identity=34.8571428571429, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921000, Length=175, Percent_Identity=34.8571428571429, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921018, Length=196, Percent_Identity=29.5918367346939, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583165, Length=182, Percent_Identity=28.021978021978, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27586; Mature: 27455

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYETILLETHGRVGLITLNRPQALNALNAQLVSEVNRALDGFEADANIGCIVITGSTKA
CCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCE
FAAGADIKEMADLTYPQIYMDDLFSDSDRVANRRKPIIAAVNGFALGGGCELALMCDFIL
EECCCCHHHHHHCCCCHHHHHHHCCCCHHHHHCCCCEEEEECCEEECCCCEEEEEEEEEE
AGDNAKFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCLSGRMIDAVEAERCGIVARIV
ECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEC
PSAELLDEALKVAAVIASKSLPITMMVKESVNRAFEVNLTEGVRFERRVFHAAFATQDQK
CCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCHH
EGMAAFVAKRAPEFQGK
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYETILLETHGRVGLITLNRPQALNALNAQLVSEVNRALDGFEADANIGCIVITGSTKA
CCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCE
FAAGADIKEMADLTYPQIYMDDLFSDSDRVANRRKPIIAAVNGFALGGGCELALMCDFIL
EECCCCHHHHHHCCCCHHHHHHHCCCCHHHHHCCCCEEEEECCEEECCCCEEEEEEEEEE
AGDNAKFGQPEINLGVLPGMGGTQRLTRAVGKAKAMEMCLSGRMIDAVEAERCGIVARIV
ECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEC
PSAELLDEALKVAAVIASKSLPITMMVKESVNRAFEVNLTEGVRFERRVFHAAFATQDQK
CCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCHH
EGMAAFVAKRAPEFQGK
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]